급성 심근허혈에 의해 윤발된 승모판 폐쇄부전의 기전에 관한 실험적 연구

강덕현,송현,송재관,김현숙,이재환,임희영,이재원,박성욱,박승정,송명근 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.8

판막 질환에서의 심근 섬유화 : 심근 조직을 이용한 형태학적 연구 및 혈청 생화학적 지표의 분석

강수진,박덕우,송재관,박경민,송종민,강덕현,한기훈,송현,이재원,주석중,송명근,김정선 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

정상 인체 멜라닌세포 및 B-16 흑색종 세포주에서 상지(桑枝) 추출물의 티로시나제 활성 억제 효과에 관한 연구

송무현,황재영,박영립,황규왕 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

Background : Recently, the undesirable effects of UV exposure have been increasing because of destruction of ozone layer and excessive solar exposure in enjoying leisure. Therefore, the chance to have uneven skin pigmentations has been increasing. To keep away from the unwanted skin pigmentation, melanogenesis inhibitors have been developed for use in cosmetic preparations for the purpose of skin whitening. Plant extracts having an inhibitory effect in melanin synthesis may be a good choice as a cosmetic ingredient because they have relatively fewer side effects. Objective : In this study, the inhibitory effects of ramulus mori (young twigs of Morus alba L) on tyrosinase activity were investigated cultured normal human melanocytes and B-16 melanoma cells by using enzyme assay and RT-PCR. Methods : Tyrosinase activity was determined by spectrophotometry. The effects of whitening agents (kojic acid, arbutin, licorice extracts and ramulus mori extracts) on mushroom tyrosinase was compared by measuring the IC_(50), the concentration of the compound at which half of the original tyrosinase activity is inhibited. Normal human melanocytes taken from neonatal foreskin and B-16 melanoma cells, tyrosinase activity inhibition was measured by spectrophotometry. We observed tyrosinase volume in B-16 melanoma cells by using PT-PCR. Results : Ramulus mori extracts showed strong inhibitory effect on tyrosinase activity in both normal human melanocytes and B-16 melanoma cells. We also confirm that they have a inhibitory effect on tyrosinase expression in the RT-PCR. Conclusion : This study showed that ramulus mori extracts had strong inhibitory effect against tyrosinase activity. The results suggests that ramulus mori extracts can be used as a new whitening agent.

농촌지역 노인들의 우울증상에 관련된 요인들의 특성

송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2

This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.

Helicobacter pylori의 생육과 각종 유기산의 식품 보존료로서의 효과

송재철,조은경,박현정,신완철,최석영 울산대학교 2002 생활과학논문집 Vol.3 No.2

Helicobacter pylori의 생육과 각종 유기산의 식품보존료로서의 효과를 검토하였다. 우선 보존료로 현재 식품첨가물로 사용되고 있는 소르빈산(sorbic acid), 프로피온산(propionic), 안식향산(benzoic acid) 등의 H. pylori에 대한 항균능력을 검토한 결과 소르빈산, 프로피온산, 안식향산의 H. pylori에 대한 항균효과가 관찰되었으며 안식향산은 소르빈산이나 프로피온산과 비교했을 때는 별로 큰 효과를 나타내지 못하였다. 보존료의 조합에 대한 H. pylori의 생육억제효과는 0.5% 소르빈산과 0.5% 프로피온산을 조합하는 경우 나타났으며 다른 조합군과 비교할 때 큰 차이를 나타내지 못하였다. 유기산과 H. pylori의 생육관계에서는 단독 첨가시에는 항균력이 크게 나타나지 않고 조합하는 경우에만 좋은 항균력을 나타내었다. 유기산, 보존료와 H. pylori 생육관계는 유기산류는 어떤 보존료와 혼합하여도 항균력에 상승효과가 있는 것으로 나타났다. 특히 구연산의 경우 소르빈산과 조합한 경우에는 H. pylori생육이 많이 억제되었으며 그 다음이 구연산+안식향산, 구연산+프로피온산 순으로 균에 대한 항균력이 감소되었다. 또 사과산과 숙실산은 구연산과 비슷한 경향을 나타내었다. The purpose of this study was to examine the effect of various organic acids as food preservatives on the growth of Helicobacter pylori(H.pylor). The antimicrobial effects of sorbic acid, propionic acid and benzoic acid currently used as food preservatives were examined in light of the growth of Helicobacter pylori. Each of them was observed to have positive antimicrobial effect on Helicobacter pylori. However, benzoic acid wasn't higher than sorbic acid and propionic acid in antimicrobial effect. As for the combination of preservatives, antimicrobial activity of H. pylori was shown in the combination of sorbic acid(0.5%) and propionic acid(0.5%). However, its effect was not significantly different in comparison with other combinations. In the relationship of organic acids and H. pylori's growth, organic acid had no effect on antimicrobial activity when it was used alone, whereas antimicrobial effect was shown to be better in case of combination. If intermix of organic acids and preservatives and addition to the medium for suppression of H. pylori growth were achieved, organic acid exhibited synergistic effect on suppression of H. pylori's growth, even in combination of any other preservatives. In particular, in combination of citric acid and sorbic acid, the growth of H. pylori was shown to be remarkable suppression. And the effectiveness on suppression of H. pylori's growth was lowered in order of citric acid + bezoic acid and citric acid + propionic. acid. It was also found that the antimicrobial effects of malic acid and succinic acid were similar to those of citric acid.

AGT 시스템의 동적 증가계수 시뮬레이션

송재필,김기봉,정인근,김현호 中央大學校 建設環境硏究所 2002 環境科學硏究 Vol.13 No.2

무인궤도운전열차(Automated Guide-way Transit, AGT) 시스템의 접지력 및 교량-차량의 동적 응답을 구하기 위해 노면조도가 있는 주행로를 운행하는 AGT 시스템에 대한 동적 운동방정식을 유도하였다. AGT 시스템의 차량은 3차원 11 자유도 모델로 이상화하였고, 교량은 3차원 프레임 모델로 단순화하였다. 동적 운동방정식의 해를 구하기 위하여 컴퓨터 프로그램을 개발하였고, 해석된 결과는 실험자료의 결과치와 비교 분석하였다. 본 연구에서 개발된 프로그램의 해석결과 AGT 교량, 차량 그리고 노면요철의 상호 작용에 의한 교량과 차량의 동적 응답을 구하여 정밀한 교량의 동적증가계수를 산정할 수 있었다. The equations of motion for an automated guide-way transit(AGT) system running on a path with roughness have been derived to investigate dynamic responses and wheel loads of moving vehicles of the AGT system. A vehicle of the AGT system is idealized as three-dimensional model with 11 degree-of-freedom, and a bridge is modelized with three F.E. method. The computer program is developed to solve the dynamic equations, and analytical results are verified by comparing the results with experimental ones. As a result, the computer program which developed in this study can be used for estimate the dynamic responses of bridge and vehicle due to dynamic interaction of bridge, vehicle and surface roughness, and with these results the dynamic increment factor of bridge can be obtained.

열처리 및 보상이온의 주입에 의한 α-수정내의 S₁,S₁',S₁˝ 공명중심들의 변화

송광섭,최재현,최 덕,최옥천,이재훈 명지대학교 자연과학연구소 1995 자연과학논문집 Vol.12 No.-

수정시편을 300℃, 400℃,500℃,530℃에서 각각 20시간씩 열처리한 후에 X밴드 전자 공명장치를 사용하여 α-수정내의 S?(1).S?',S?'' 세 공명중심들에 대한 열처리 조건에 따른 공명 신호들의 세기의 변화를 상온에서 조사하였다.열처리 효과로 S?'과 S?'' 공명중심들이 일부 S?(1)으로 전환되었다. 480℃에서 수정시편의 c축 방향으로 15ooV/cm의 전기장을 가하여 시편내에서 +1가의 보상이온들을 빼내거나, 500V/cm 와 1000V/cm의 전기장으로 Li+ 이온과 Na+이온을 각각 주입시킨 후에 공명신호들의 세기를 서로 비교하였다.한편 시편내에서 보상이온들을 빼내지 않고 400℃에서 20V/cm 와 50 V/cm의 약한 전기장으로La+이온을 주입시킨 경우와 200V/cm의 전기장으로 Na+이온을 주입시킨후에 공명신호들의 세기가 변화하는 모습을 조사하였다.Li+ 이온이 시편에 주입됨에 따라 세 공명중심들 모두 공명신호들의 세기가 증가하였으며, Na+이온이 주입된 경우에는 공명신호를의 세기가 크게 감소하였다. 열처리 효과와 전기장 처리의 결과로부터 세 공명중심들 모두 Li+이온에 의하여 전하가 보상되는 것으로 판명되었다. After heating a quartz sample for 20 hours at 300℃, 400℃, 500℃, and 530℃, respectively, the changes of resonances signal intensities of the centers S?(1), S?', and S?''in α-quartz according to the conditions of heat treatments were investigated at room temperature by employing an X-band electron spin resonance spectrometer. The centers S?' and S1'' were partly converted into S?(1) by the effect of heat treatments. After extracting positively monovalent ions from quartz samples by applying an electric field of 1500V/cm along the crystal c-axis at 480℃ and after electrodiffusing Li+ ions and Na+ ions into the samples with applied electric fields of 500 and 1000 V/cm, respectively, the intensities of resonance spectra were compared with each other. On the other hand a quartz sample was electrodiffused with Li+ ions at 400℃ with weak electric fields of 20 and 50V/cm and then implanted with Na+ions with an electric field of 200V/cm.As Li+ ions diffused into the sample, the intensities of all the three centers increased. But in the case of electrodiffusion with Na+ ions the resonance signals decreased by considerable. From the effect of heat treatment and the result of electrodiffusions it is proved that all the three centers are compensated by the Li+ion.

Helicobacter pylori의 생육에 영향을 미치는 성분과 식품첨가물에 관한 연구

송재철,정혜진,박현정,조은경,신완철 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.5

본 연구에서는 일반적으로 섭취하고 있는 식품재료나 음식 중에 공통적으로 함유한 성분 또는 가공중에 많이 첨가되는 성분을 선정하여 이들이 Helicobacter pylori 생육에 어떤 영향을 미치는지를 검토하였다. 첫째, 일반식품의 주요 성분인 탄소원, 질소원, 무기질을 대상으로 Helicobacter pylori 생육에 미치는 영향을 검토하였다. 그 결과 식품성분 중 탄소원의 종류에 따라 균일 생육활성은 다르게 나타났다. 우선 단당류가 이당류나 다당류에 비교하여 생육을 촉진하였고 특히 단당류 중 포도당은 가장 균의 생육에 큰 영향을 미치는 것으로 나타났다. 그러나 만노오스는 균의 생육을 오히려 억제시키는 것으로 나타났다. 당알코올류인 솔비톨, 만니톨, 말티톨, 크리시톨 등은 균의 생육에 아무런 영향을 미치지 않은 것으로 나타났다. 질소원과 Helicobacter pylori의 생육과의 관계는 평소 식생활에서 많이 섭취된다고 생각되는 일반 음식에 많이 함유되어 있는 유기형 아미노산 성분고 무기형 질소화합물을 대상으로 균의 생육 정도를 비교하였다. 리신, 메티오닌, 시스테인, 트립토판 등은 균의 생육을 촉진하고 아르기닌, 황산암모늄, 염화암모늄 등은 균의 생육에 도움이 되지 않고 암노니아, 요소등은 균의 생육에 도움이 되지 않고 암모니아, 요소 등은 균의 생육에 조금 영향을 미침을 관찰할 수 있었다. 특히 리신과 메티오닌은 가장 균의 생육에 영향을 미치는 것으로 나타났다. 무기염류의 영향에서는 황산마그네슘, KH-2PO_4, 염화마그네슘 등은 큰 영향을 미치지 못하였고 황산철, 염화제일철, 염화제이철은 생육을 억제하는 것으로 나타났다. 식품첨가물의 Helicobacter pylori의 생육 정도를 검토한 것으로, 그 다음이 질화나트륨, 염화나트륨 순이었으며 토코페롤은 전혀 생육에 영향을 미치지 못하였다. The purpose of this study was to choose principal food components contained in diet foods and food additives used for manufacturing processed foods, and elucidate their in vivo effects on the growth pattern of Helicobacter pylori. To do this, the antibacterial effects of various sources of carbon, nitrogen and mineral as an effect agent on Helicobacter pylori were first assessed based upon bacterial growth degree. Results show that the source of carbon tested had different effects on bacterial growth of Helicobacter pylori. It was revealed that a promotional effect of monosaccharides resulted in enhanced growth of Helicobacter pylori compared with disaccharides and polysaccharides. In particular, glucose was observed to be most effective in growth of Helicobacter pylori among monosaccharides tested, whereas mannose to hinder the growth of Helicobacter pylori. Polyols such as sorbitol, mannitol, maltitol and xylitol was, however, observed to show no promotion or suppression effect on growth of Helicobacter pylori. Apart from these, the sources of amino acid and inorganic nitrogen were chosen and tested to assess the promotion or suppression effect of nitrogen sources on growth of Helicobacter pylori. It was found that amino acid such as lysine showed its promotion effect on the growth of Helicobacter pylori, while arginine, (NH_1)_2SO_4 and NH_4Cl showed no effect on its growth. Ammonia and urea were, however, observed to have a positive effect on the growth of Helicobacter pylori. Among these effect agents, lysine and methionine were revealed to show the most positive effect on growth of Helicobacter pylori. Minerals such as MgSO_4, KH_2PO_4, and MgCl_2 appeared to exert their positive growth effects, whereas CaCl_2, and CaSO_4 had a little effect. In addition FeSO_4, FeCl_2, and FeCl_3 brought suppression on the growth of Helicobacter Pylori. In studies of the growth of Helicobacter pylori by food additives, ascorbic acid showed extreme suppression on its growth. Sodium nitrate and sodium chloride were also found to be of negative effect on the growth of Helicobacter pylori in order of degree, whereas tocopherol had nothing to do with microbial growth.

Plant Regeneration and Bulblet Formation of Allium wakegi Araki

Song,Won-seob,Yang,Deok-Chun,Yoon,Jae-Ho,Ryu,Sang-Hyun 한국자원식물학회 2004 Plant Resources Vol.7 No.1

Allium wakegi was cultured shoot tip in the condition of light culture. The Allium wakegi added plant growth regulator was observed of plant regeneration and bulblet formation. Callus Induction and growing rate was the best of 78% when added alone 2,4-D 0.5mg/L. In the formation of shoot, its regeneration rate was 96% when added BA 0.5mg/L in the light culture condition. When BA 0.5mg/L and NAA 0.5mg/L mixed and BA 0.5 mg/L and NAA 1.0mg/L mixed, the rates were 99% and 97% respectively, and these conditions were suitable for forming shoot. In the formation of roots, when added NAA 2.0mg/L in the light culture condition, the regeneration rate was 90.6 % and the roots were abnormal. When added NAA 1.0mg/L, the rate was 82 % and the highest. In the formation of bulbs, when BA 05mg/L and NAA 1.0mg/L mixed, the root generantion and its size in the bulbs was the best compare to other treatment experiments.

Sialic Acid 의 감소가 탈지분유의 단백 용해도에 미치는 영향

송재철,박현정,이재영 成均館大學校 科學技術硏究所 1987 論文集 Vol.38 No.2

The milk protein portion contains sialic acid which may be used as an index for k-casein, In comparison with non fat dry milk(NFDM) and calcium caseinate, the solubility of NFDMprotein isolates at the various pH was inferior. Another consideration for the low solubility relates to the possibility that k-casein may have been extracted or possibly modified by the action of alcohol. The k-casein is the principal stabilizing fraction for casein micelle and any changes which result in its destruction or removal would be expected to have potent results. During solvent treatment, 16.5% of sialic acid was extracted in the first extraction solvent, whereas the second treatment caused only a 4.0% loss, based on sialic acid analysis. A study was conducted on the effect of concentration of methanol on loss of sialic acid. The loss of sialic acid decreased as the concentration of methanol increased to above 62% in bath the first and second extracted solvent. it appears that loss of sialic acid is attributed to the water fraction rather than the methanol fraction. The effect of addition of the dried extracted solids on solubility was investigated. Protein solubility of NFDM protein isolates was sharply increased by 10% addition of the extracted solids and, thereafter, decreased. Efforts to restore solubility by feeding-back the extracted material supported the concept that removal of surface k-casein may have been a possible factor.