경도관 동맥색전술을 이용한 비정상 자궁출혈의 치료

양승부,최교창,이상진,정영진,임한혁,한효상,황인철,조환성 순천향의학연구소 2005 Journal of Soonchunhyang Medical Science Vol.11 No.1

Objective : Our purpose of this study is to evaluate the effectiveness of the transcatheter arterial embolization for the management of abnormal uterine bleeding. Materials and Methods : 14 patients with massive or recurrent uterine bleeding underwent percutaneous transcatheter arterial embolization between February 2003 and September 2004. We reviewed 14 cases of uterine artery or internal iliac artery embolization using gelfoam or PVA particles. Results : Good management of uterine bleeding was achieved in 13 of 14(93%) cases. The cause of abnormal uterine bleeding was myoma(5 case), post D & E bleeding(3), acquired vascular malformation(3), pseudoaneurysm(1), adenomyosis(1), and endometrial hyperplasia(1). Conclusion : Transcatheter artery embolization is an effective and life-saving procedure in massive or recurrent uterine bleeding. Early diagnosis and prompt transcatheter arterial embolization is a useful mangement of uterine bleeding.

Vibrio vulnificus 박테리오 파아지의 특성

오양효,윤소겸,공은진,김민정,김영부,박영민 대한감염학회 1995 감염 Vol.27 No.5

목 적 : 이미 보고되어 있는 파아지들의 경우, 일반적인 형태, 숙주역과 몇 가지의 성질만 연구되어 있으므로 보다 상세한 특성에 대한 연구가 필요한 상태이다. 본 연구에서는 해양으로부터 분리한 V. vulnificus를 숙주균으로 하여 투명한 용균반을 형성하는 파아지를 분리해 낸 다음, 분리된 V. vulnificus 파아지의 특징을 연구하고자하였다. 방 법 : V. vulnificus 를 숙주균으로 하여 해산물로부터, 숙주균에 특이적인 파아지를 분리하고, 농축 정제과정을 거쳐 파이지입자를 순수분리하였다. 파아지의 형태를 전자현미경으로 관찰하였으며, 분리된 파아지의 숙주특이성을 조사하고, 파아지 입자의 물리적 특성을 검토하기 위해, 온도 안정성시험, pH 안정성 시험을 실시하였다. 파아지의 흡착율, 파아지의 흡착율에 미치는 무기염 이온의 영향, 온도의 영향과 pH의 영향을 관찰하였다. 파이지의 일단증식곡선을 구하였으며 토끼 면역과 교차 중화 시험, 파아지 핵산의 특성, 구조단백질의 특성을 조사하였다. 결 과 : 전자현미경관찰결과 본 연구에서 분리한 파아지는 두부와 미부를 가지는 형태로 미부에 꼬리판과 꼬리섬유를 가지고 있었다. 파아지의 물리적성질시험 결과 파아지는 50℃이하의 온도에서 안정하였으며 중성pH영역에서 안정하고 산성과 알칼리 영역에서는 활성을 잃었으며 자외선에는 35초 조사에 50% 정도 불활성화 되었다. 파아지는 약 65%의 흡착율을 나타내었으며 흡착에 ??, ??을 요구하였다. 파아지입자가 안정한 온도와 pH영역에서는 흡착에 영향을 받지 않았으며 일단증식시험 결과 108 PHU/ml의 파아지 입자를 생산하였다. 정제한 파아지를 항원으로 하여 토끼에 면역한 항혈청에 파아지가 중화되었으며 파아지 핵산은 이중나선 DNA였다. 파아지의 구조단백질은 SDS-PAGE polyacrylamide gel 전기영동 결과 2개의 밴드를 나타내었다. 그리고 Western blotting 결과 파아지의 전기영동 결과와 동일한 위치의 밴드에서 항원성을 나타내었다. 결 론 : 인체병원균으로 중요성이 인식되고 있는 V. vulnificus는 해산물의 위생과 국민건강에 직접적인 영향을 미칠 수 있는 세균으로 이에 대한 활발한 연구가 요구되는 실정이다. 파아지의 분리율은 숙주균의 분리율에 의존하므로 숙주균의 해수내 분포에 영향을 미친다고 추측되며, 파아지는 숙주균에 감염되어 숙주균을 용균시키므로 숙주균의 개체수를 조절하여 생태학적 측면에서 중요한 기능을 담당하는 것으로 여겨진다. Background : Vibrio vulnificus causes severe wound infection and life-threatening septicemia. In this study we present the characteristics of V. vulnificus bacteriophage and the basis of pathogenicity study of V. vulnificus related on bacteriophage. Methods : We isolated bacteriophage for V.vulnificus from marine products and examined its physiological and biochemical characteristics, and the ultrastructure by electron microscopy (TEM,SEM). And also, we investigated host ranges, temperature stability, pH stability, inactivation by UV irradiation, DNA isolation and analysis and structural protein analysis of the bacteriophage. Results : The adsorption rate of bacteriophage was above 80% and had a little influence at 10-45℃ and pH5-10. Isolated bacteriophage was used to produce antiserum against rabbits and its antigenicity was investigated by cross-neutralization. Western blot analysis showed the capsid protein of bacteriophage had antigenicity. The bacteriophage had double-strand DNA. In restriction analysis, the phage DNA was digested HinfI. The structural protein of phage showed two bands of 34.7kDa and 18.4kDa on SDS-PAGE. Conclusion : The specificity of bacteriophage may be due to the specific binding site that be possessed by host strain surface. Therefore, it seems that virulent bacteriophage controls the number of host strain and then takes a role on natural marine environment.

고정화 세포를 이용한 황화수소 제거공정 최적화

박양호,손효진,김경주,권규혁,이중헌 한국공업화학회 2003 응용화학 Vol.7 No.1

In this study, the hybitd reactor has used to remove H_2S from the toxic gas. Since the oxidation and reduction reactors were combined, theri sizes were important to stabilize developed process. The optimal sizes of oxidation and reduction reactors were determined considering iron oxidation rate and H_2S removal rate. The optimal ratio of reactor size was 4 L for oxidation reactor to 1 L for reduction reactor.Immobilized Thiobacillus ferrooxidans has been developed to minimize the size of oxidation reactor. Since the immobilized cell enhanced the oxidation rate, it was possible to incrase H_2S removal efficiency. It was possible to incerase operation time because immobilized cell can survie lger than free cell.

철 산화균(Thiobacillus ferrooxidans)을 이용한 황화수소 제거-온도변화에 따른 황화수소 제거효율변화

박양호,손효진,김려화,권규혁,이중헌 한국공업화학회 2002 응용화학 Vol.6 No.2

Effects of temperature on hydrogen sulfide removal efficiency has been studied in this research. Two reactors which combined iron oxidization reactor and reduction reactor were manufactured. Iron oxidation reactor was used to oxidize Fe(II) to Fe(III) with Thiobacillus ferrooxidans and the hydrogen sulfide was oxidized to make element sulfur with the reduction of iron. The optimal temperature for the iron oxidation was 30℃ however that of iron reduction was 40℃. From this result, the optimal continuous operation conditions has been determined.

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

Epidermoid Cyst in an Intrapancreatic Accessory Spleen in the Pancreas Head: A Case Report

Hyo Jung Ko,Kwangho Yang,Jae Ryong Sim,Tae Beom Lee,Byung Hyun Choi,Je Ho Ryu,Jung-Hee Lee 대한외과학회 2021 대한외과학회 학술대회 초록집 Vol.2021 No.11

한국의 라미부딘 내성 B형간염 환자에서 아데포비어 내성 발생의 위험 인자에 관한 연구

Hyo Joon Yang,Jeong Hoon Lee,Won Kim,Yoon Jun Kim,Jung Hwan Yoon,Hyo Suk Lee 대한간학회 2008 Clinical and Molecular Hepatology(대한간학회지) Vol.14 No.3(S)

Role of Ganglioside GD1a on the Differentiation of Human Mesenchymal Stem Cell into Osteoblast

Hyo-Jung Yang,Jae-Sung Ryu,Jung-Woo Jin,Jin-Hyoung Cho,Eun-Jeong Jeong,So-Dam Lee,So-Hyun Lee,Young-Kug Choo 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

We isolated human mesenchymal stem cells (hMSC) from dental pulp. These cells are multipotent and serve as precursors for various mesoderm-type cells such as osteoblasts, chondroblasts and adipocytes, as well as several other types of cells. This study investigated the possible role of gangliosides in osteoblast differentiation of hMSCs. Gangliosides, which exist in glycosphingolipid-enriched domains on the cell membrane The role of gangliosides that play in osteoblastogenesis is not yet clearly understood. Therefore, in this study, We investigated the relationship between gangliosides and osteoblast differentiation of human mesenchymal stem cells (hMSCs). The results of high-performance thin-layer chromatography (HPTLC) showed that ganglioside GD1a expression was increased during the differentiation of hMSCs into osteoblasts. Furthermore, we showed that involvement of alkaline phosphate (ALP) with GD1a by immunostaining. Taken together, these results suggest that gangliosides GD1a may play a role in the osteoblast differentiation process of hMSCs.

Down-regulation of GD1a decreases osteoblast differentiation from human dental pulp-derived stem cells

Hyo-Jung Yang,Jung-Woo Jin,So-Hyun Lee,Jae-Sung Ryu,Jin-Hyoung Cho,So-Dam Lee,Young-Kug Choo 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Human dental pulp-derived stem cells (hDPSCs) have been considered alternative sources of adult stem cells because of their potential to differentiate into osteoblasts, chondroblasts and adipocytes. The osteoblast is a mononucleate cell that is responsible for bone formation. Gangliosides are key lipid molecules that are required for the regulation of cellular processes such as proliferation and differentiation. The role of gangliosides that play in osteoblastogenesis is not yet clearly understood. Therefore, in this study, we demonstrated the clearly role of gangliosides in the osteoblast differentiation of hDPSCs by shRNA. The results of high-performance thin-layer chromatography (HP-TLC) showed that ganglioside GD1a expression was increased during the differentiation of hMSCs into osteoblasts. We showed a localization of ganglioside GD1a with ALP by immunofluorescence staining. Biosynthesis of ganglioside GD1a synthesize with the formation of sialic acid from GM1a by ST2 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3Gal2). We used lentivirus-mediated shRNA expression for knockdown of ST3Gal2 gene expression. Down-regulation of ganglioside GD1a was decreased GD1a expression and ALP activity. Furthermore, these data indicate that the knockdown of ST3Gal2 by shRNA is inhibition of ERK1/2-phosphorylation. Taken together, these results suggest that ganglioside GD1a may play a role in the osteoblast differentiation process of hDPSCs.

Genome-Wide Association Study of Ulcerative Colitis in Koreans Suggests Extensive Overlapping of Genetic Susceptibility With Caucasians :

Yang, Suk-Kyun,Hong, Myunghee,Zhao, Wanting,Jung, Yusun,Tayebi, Naeimeh,Ye, Byong Duk,Kim, Kyung-Jo,Park, Sang Hyoung,Lee, Inchul,Shin, Hyoung Doo,Cheong, Hyun Sub,Kim, Lyoung Hyo,Kim, Hyo-Jong,Jung, Oxford University Press 2013 Inflammatory bowel diseases Vol.19 No.5

<P>Recent genome-wide association studies and meta-analyses have identified 47 susceptibility loci for ulcerative colitis (UC) in Caucasian populations. A previous genome-wide association study of UC in a Japanese population suggested marginal sharing of susceptibility loci between Caucasian and Asian populations. We performed a genome-wide association studies to identify UC susceptibility loci in a Korean population and further comparative study.</P>