2G-Bayer CFA 패턴을 위한 칼라 영상 보간 방법 ( A Color Interpolation Technique for 2G-Bayer CFA Pattern )

한효상,김춘우 대한전자공학회 1998 대한전자공학회 학술대회 Vol.1998 No.1

참당귀, 중국당귀, 일당귀 열수 추출물의 RAW 264.7 대식세포에서 IL-1β, TNF-α, iNOS 유전자 차등 발현 연구

한효상,홍성균,Han, Hyo-Sang,Hong, Seong-Gyun 한국디지털정책학회 2017 디지털융복합연구 Vol.15 No.11

We tried to analyze the inflammation reactions by treatment of AG, AS and AA in murine RAW 264.7 cells. To investigate the effect of AG, AS and AA on cell viability of RAW 264.7 cells, AG, AS and AA were treated for 24 h and MTS assay was performed. Cell viabilities were increased in $1,600{\mu}g/ml$ concentration by AS, AA and AG treatments, respectively. The mRNA expression levels of $IL-1{\beta}$, $TNF-{\alpha}$ and iNOS were increased by AG and AA treatment at a concentration of $200{\mu}g/ml$ in RAW 264.7 cells without Lipopolysaccharide (LPS) treatment. The mRNA expression levels of $IL-1{\beta}$, $TNF-{\alpha}$ and iNOS were increased by AG and AA 6 h treatment at a concentration of $200{\mu}g/ml$ with LPS treatment. In this study, we observed that AG, AS and AA show various activities on inflammation reaction depend on their treatment time. In the future, studies should be conducted to investigate the effects of AG, AS and AA on the various inflammatory responses of macrophages. Murine macrophage cell line인 RAW 264.7 대식세포에 참당귀(AG), 중국당귀(AS), 일당귀(AA)를 6시간과 24시간을 반응시킨 뒤 시간별로 나타나는 inflammation 반응을 확인하고자 하였다. LPS-induced RAW 264.7 대식세포에 AG, AS, AA를 6시간 반응 시킨 경우 $IL-1{\beta}$, $TNF-{\alpha}$, iNOS의 mRNA 발현량을 증가시켰고, AA에서 더 높은 Anti-inflammatory effect를 확인하였다. AG, AS, AA가 RAW 264.7 대식세포의 cell viability에 미치는 영향을 확인하기 위해 MTS Assay(24시간)를 수행한 결과 $1,600{\mu}g/ml$ 농도에서 모두 증가시켰다. AG, AS, AA가 LPS를 처리하지 않은 RAW 264.7 대식세포에 6시간동안 면역반응에 미치는 영향을 확인한 결과 AG와 AA를 $200{\mu}g/ml$ 농도로 처리한 RAW 264.7 대식세포에서 $IL-1{\beta}$, $TNF-{\alpha}$, iNOS의 발현이 증가되었다. 이 연구를 통해 AG, AS, AA는 RAW 264.7 대식세포에 LPS를 처리하였을 때 inflammation 반응을 촉진하며, 24시간 뒤 inflammation의 억제를 유도한 결과를 얻을 수 있었다. 향후 참당귀, 중국당귀, 일당귀 열수 추출물이 대식세포의 다양한 염증 반응 메커니즘에 미치는 작용을 확인하는 연구가 필요하다.

Effect of Sparasis crispa Extract on 5α-Reductase Gene in Dermal Papilla Cells

한효상 사단법인 미래융합기술연구학회 2021 아시아태평양융합연구교류논문지 Vol.7 No.7

Sparasis crispa (Cauliflower Mushroom) is widely used as a cosmetic product to promote hair growth, and most research on mouse hair growth has been reported. However, the study of the effects of Sparasis crispa on dermal papilla (DP) cells, which play an important role in hair growth, is not well known. For this reason, the researchers studied the effect of Sparasis crispa on DP cells. The strong antioxidant activity of Sparasis crispa was confirmed by ABTS analysis. In MTS analysis, 2 mg/mL of Sparasis crispa was treated, reducing cell survival to 160.2% in DP cells and no cytotoxicity was observed at 1 mg/mL concentration. The level of mRNA expression of the BMP6, FGF7, FGF10, CTNNB1, SRD5A1, SRD5A2 involved in hair cycle and hair loss induction was measured as quantitative RT-PCR after the Sparasis crispa treatment. Significant change in mRNA expression of type Ⅰ 5 alpha-reductase could be identified if Sparasis crispa with a concentration of 0.5 mg/ml was treated in DP cells. Additionally, type Ⅱ 5 alpha-reductase mRNA expression was found to have an effect increasing to 1.38 fold. It is not yet known that the treatment of Sparasis crispa increases the expression of type Ⅰ and type Ⅱ 5 alpha-reductase mRNA in the DP cells, which can have an excellent treatment effect for Sparasis crispa male hair loss. However, Sparasis crispa increased the mRNA expression of BMP6, FGF7 and CTNNB1 to promote hair production. For this reason, the effect of Sparasis crispa treatment can help in the action, which the final hair generation cycle requires.

자화지정 추출물이 LPS로 유발된 대식세포의 염증인자에 미치는 영향

한효상 한국디지털정책학회 2018 디지털융복합연구 Vol.16 No.7

본 연구는 지질다당류에 의해 유도된 대식세포주인 RAW 264.7 세포에서 자화지정 추출물이 염증 매개체 생성에 미치는 영향을 조사하고자 하였다. 자화지정 추출물이 대식세포주인 RAW 264.7 세포의 세포 생존 능력에 미치는 영향을 조사 하기 위하여 MTT assay를 실시하였다. 또한, Bio-Plex 사이토카인 분석(cytokine assay)을 통하여 NO, 인터루킨 (IL) -1β, 종양 괴사 인자(TNF-α) 및 IL-6와 같은 다양한 사이토카인(cytokine)의 농도에 의한 자화지정 추출물의 항염증 효과를 조사 하였다. 자화지정 추출물은 LPS로 유도 된 대식세포에서 NO, IL-1β, TNF-α 및 IL-6의 농도를 25㎍ / mL 이상으로 유의하게 저해하였으며 세포 생존율에는 변화가 없었다. 이러한 결과는 자화지정 추출물이 LPS로 유도된 대식세포에서 IL-1β, TNF-α 및 IL-6와 같은 염증성 사이토카인(cytokine)의 억제와 관련된 항염증 효과를 갖는다는 것을 시사한다. 앞으로 자화지정을 이용한 염증질환에 관련된 치료제개발에 새로운 연구가 더 필요한 바이다. The purpose of this study was to investigate the effects of Violae Herba Water Extract (VH) on the proinflammatory factors of lipopolysaccharide (LPS)-induced on the production of inflammatory mediators in RAW 264.7 mouse macrophages cells. We examined effect of Violae Herba Water Extract on the cell viability of RAW 264.7 mouse macrophages cells. Futhermore, After 24 hours treatment we investigated anti-inflammatory effect of Violae Herba Water Extract by the production of Bio-Plex cytokine assay, concentrations of various cytokines such NO, interleukin(IL)-1β, tumor necrosis factor α(TNF-α) and IL-6. The water extract of Violae Herba significantly inhibited the production of NO, IL-1β, TNF-α and IL-6 at the concentration of 25, 50, 100 and 200 ㎍/mL in the LPS-induced RAW 264.7 mouse macrophages cells with no changes in the cell viability of them. These results suggest that water extract of Violae Herba has anti-inflammatory effect related with its inhibition of proinflammatory cytokines such as IL-1β, TNF-α and IL-6 in the LPS-induced RAW 264.7 mouse macrophages cells. Further research is needed to develop therapeutic agents for inflammatory diseases using Violae Herba.

육두구의 形態 鑑別에 관한 硏究

韓孝尙,吉基正,李暎鍾 대한본초학회 2005 大韓本草學會誌 Vol.20 No.1

Objectives : Myristicae Semen is the seed of Myristica fragrans Houttuyn. Recently, the seed of Myristica argentea Warburg is also sold as a fake of Myristicae Semen. Thus, in order to distinguish Myristica fragrans Houttuyn and Myristica argentea Warburg, their external, internal, and flour states were observed via microscopic examination. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and the flour of it was mounted by the liquid made by the same ratio of each of glycerin, acetic acid, and water, and then observed and photographed by olymphus-BHT. Result 1. The seed of Myristica fragrans Houttuyn is oval with a length of 2~3cm and diameter of 1.5~2.5 cm, while the seed of Myristica argentea Warburg is of a longer oval shape with a length of 2.5?3.5cm and diameter of 1.5cm. 2. There was no difference between the internal parts of the cross-sections of Myristica fragrans Houttuyn and Myristica argentea Warburg. 3. There was no difference between the flour states of Myristica fragrans Houttuyn and Myristica argentea Warburg. Conclusion : There was a distinctive difference between the external forms of Myristica fragrans Houttuyn and Myristica argentea Warburg, while there was no significant difference in their internal and flour states. This makes it difficult to distinguish them via microscopic examination, when cut by cross-sections or grounded into flour states. Therefore, they should be distinguished through TLC, or some research on the characterising compound is needed.

조기수유 활성화를 위한 활동

한효상,추은희,백형숙,최진아,황태희,김진아,권보라,이선영 한국의료QA학회 2007 가을학술대회 Vol.2007 No.3

충청남도지역 민간임대주택과 공공임대주택간 주거환경 만족도 영향요인 연구

한효상 사단법인 한국융합기술연구학회 2023 아시아태평양융합연구교류논문지 Vol.9 No.11

본 연구의 목적은 충청남도 내 민간임대주택과 공공임대주택의 주거환경 만족도에 영향을 미치는 요인의 차이와 민간임대주택과 공공임대주택 거주자의 주거환경 만족도 차이를 살펴보는 것이다. 주거실태조사는 가구 특성, 주거환경 등 주거생활에 대한 내용을 국토교통부에서 매년 조사하여 제공하고 있고 본 자료는 마이크로데이터 통합서비스의 가입을 통해 제공받아 분석을 진행하였다. 민간임대주택과 공공임대주택 간 요인 차이를 살펴보기 위해 독립 t 검정을 사용하였으며, 민간임대주택과 공공임대주택 거주자의 주거환경 만족도에 영향을 미치는 요인을 살펴보기 위해 다중회귀분석을 실시하였다. 민간임대주택과 공공임대주택 간 설문 요인의 차이는 ‘공공임대주택 입주 기회’, ‘주택 보안 상태’, ‘의료시설 접근성’, ‘치안 및 방범 상태’ 영역에서 나타났으며, 주거환경 만족도에 영향을 미치는 요인은 민간임대주택과 공공임대주택 모두 ‘교육환경’, ‘치안 및 방범 상태’, ‘대기오염 수준’이 민간임대주택은 ‘의료시설 접근성’이 긍정적인 것으로 조사되었다. 충청남도 지역 민간임대주택과 공공임대주택 거주자의 주거환경 만족도에 영향을 미치는 요인은 "의료시설 접근용이성" 외 요인이 유사하므로 민간임대주택과 공공임대주택의 건설 및 도시개발 시 고려할 수 있는 근거 및 참고 자료로 제시할 수 있다. The purpose of this study is to examine the differences in factors affecting residential environment satisfaction between private and public rental housing in Chungcheongnam-do and the differences in residential environment satisfaction between private and public rental housing residents. The Residential Survey is an annual survey conducted by the Ministry of Land, Infrastructure, and Transport on residential life, including household characteristics and living environment, and this data was provided through a subscription to a microdata integration service. Independent t-tests were used to examine the differences in factors between private and public rental housing, and multiple regression analyses were conducted to examine the factors affecting residential environment satisfaction with the living environment in private and public rental housing. Differences in survey factors between private and public rental housing were found in the areas of ‘opportunity to move into public rental housing’, ‘housing security status’, ‘access to medical facilities’, and ‘security and crime prevention status’, while factors affecting residential environment satisfaction were found to be ‘educational environment’, ‘security and crime prevention status’, and ‘air pollution level’ for both private and public rental housing, with ‘access to medical facilities’ being positive for private rental housing. Since the factors affecting the residential environment satisfaction of residents of private rental housing and public rental housing in Chungcheongnam-do are similar except for “accessibility to medical facilities,” this study can be presented as a basis and reference for consideration in the construction and urban development of private rental housing and public rental housing.

서비스이익사슬 안에서 서비스접점인의 감정노동이 고객만족도에 미치는 영향에 관한 연구

한효상,안문현 글로벌경영학회 2011 글로벌경영학회지 Vol.8 No.4

This study is to explore empirically whether there is any positive difference within the existing structure of the service profit chain incorporating internal marketing from employee satisfaction to customer satisfaction in relation to the inclusion/exclusion of the concept of 'emotional labor'. To empirically examine the effect of the service encounters' emotional labor to customer satisfaction,the researchers used the sample from the service encounters of six companies in three different industries. The results of this study can be summarized as follows;it is confirmed for the first time that emotional labor has a statistically positive effect on customer satisfaction according to the types of industry and company in the structure of the service profit chain. Specifically, the impact of emotional labor on customer satisfaction was relatively high in the life insurance industry, but overall, the degree of impact tails off in the order of pharmaceutical and banking industries. This study examines the effect of emotional labor on customer satisfacion with internal marketing, customer orientation, service quality, and employee satisfaction. This study is academically meaningful in that the concept of emotional labor which has been studied in the area of personnel management, comes to be adopted to the marketing area. 본 연구에서는 내부마케팅(internal marketing)을 통한 종업원만족에서 고객만족에 이르는 기존의 서비스이익사슬(service profit chain) 구조 내에 ‘감정노동(emotional labor)’의 개념을 포함한 경우와 그렇지 않은 경우에 유의한 차이가 있는지 여부를 실증분석코자 하였다. 이를 위해 보험업, 제약업, 은행업등 3개 업종에 걸쳐 총 6개 회사의 서비스접점인(service encounter)을 대상으로 설문조사를 실시하였다. 본 연구의 결과, 감정노동이 서비스이익사슬 구조 내에서 업종별․회사별로 고객만족도(customer satisfaction)에 통계적으로 유의한 영향을 미치고 있다는 점을 확인하였다. 실증분석 결과를 회사별로 살펴보면 A생명보험, B생명보험, C제약, D제약은 감정노동이 회귀식에 포함될 경우 대체로 고객만족도를 설명하는 능력이 유의적으로 증가하였다. 그러나 은행업종에 속한 2개회사(E은행, F은행)의 경우 감정노동이 서비스제공시스템 내의 다른 개념들과 더불어 고객만족도에 영향을 주는지 여부를 설명할 수 없었다. 생명보험업의 경우 감정노동이 고객만족도에 영향을 주는 정도가높았으며, 대체로 생명보험업, 제약업, 은행업 순으로 감정노동이 고객만족도에 영향을 주는 정도가 떨어졌다. 이 연구에서는 내부마케팅, 고객지향성, 서비스품질, 종업원만족도를 포함한 서비스이익사슬 구조 내의 서비스제공시스템(service delivery system) 틀 안에서 감정노동이 고객만족에 미치는 영향에 대해살펴봄으로써, 그동안 인사관리 영역에서 주로 다루어져 왔던 감정노동 연구를 마케팅 영역으로 확대했다는데 학술적 의미를 부여할 수 있다.

세복수초(Adonis multiflora) 추출물의 항암 활성

한효상 한국자원식물학회 2015 한국자원식물학회지 Vol.28 No.5

This study aims to evaluate the antitumor effect of Adonis multiflora, one of the plants in the Ranunculaceae, on mice to which hepatoma cells were transplanted and to suggest its possibility as a candidate natural substance to replace antitumor drugs. We performed the MTT assay to assess the extract had a decrease in the growth rate of hepatoma cells depending on concentration. In particular, 100 ㎍/㎖ of the extract showed 40% of growth retardation rate. We assessed the autophagy activity to identify the inhibitory autophagy mechanism of tumor cells in the extract. This proved that the activity increases more as the concentration of the extract is higher. We conducted the Western blot test to confirmed the expression of two proteins LC3 and p62. The expression of p62 was in inverse proportion to the concentration of the extract whereas LC3-Ⅱ increased more as the concentration of the extract was higher. This showed that an increase in the autophagy relies on the conentration of the extract. We performed a test to discover the influence of the extracts on hepatoma cells transplanted to mice. The test proved that the extract triggers a significant decrease in the growth rate of tumor cells. Compared to the start of the test, the size of tumor cells with 50, 100 and 200 ㎎/㎏ of the extract respectively increased by 4, 3.7 and 3.5 times whereas in the controlling group by 6.3 times. The size of tumor cells in benign tumor controlling group increased by 3.1 times. This showed a significant decrease in the growth rate of tumor cells compared to the controlling group. We carried out the experiment of influence of the extract on the expression of two proteins LC3 and p62 in the tumor tissue transplanted into mice. The experiment showed that LC3-II increases more as the concentration of the extract is higher. However, there was a rapid decrease in p62 with 200 ㎎/㎏ of the extract compared to the controlling group. In this study, we proved that the autophagy activity of Adonis multiflora extract inhibits the growth of hepatoma cells by in vitro and in vivo experiments. In conclusion, the inhibitory autophagy mechanism of tumor cells in the extract can be used as a new treatment of antitumor. 본 연구에서는 세복수초 추출물에 대한 항암활성을 평가하고자 간암세포주인 SK-Hep1 세포주에서 MTT를 통한 세포독성을 평가하고 자가포식(autophagy) 형성정도를 확인하였다. 또한, 종양형성능 측정(Xenograft assay)를 통하여 세복수초추출물에 대한 항암활성평가를 수행하였다. 그 결과 in vivo 및in vitro에서 모두 항암활성이 뛰어나게 나타났으며, 세복수초추출물의 항암작용은 자가포식(autophagy)을 증가시키는 것으로 나타났다. 세복수초 추출물은 in vitro 및 in vivo에서 모두LC3의 발현을 농도의존적으로 증가시켜며 p62의 발현을 억제시키는 것으로 확인되었으며, 따라서 세복수초 추출물은 자가포식(autophagy) 활성을 증가시켜 암세포의 세포사멸을 유도하는 것으로 판단되어 간암치료제 개발 및 간암치료제와의 병용요법 등 새로운 작용기전의 항암신약개발 소재로서의 가능성이 있음을 제시한다.

애엽(艾葉) 발효 추출물의 면역활성에 관한 연구

한효상 ( Hyo Sang Han ),박완수 ( Wan Su Park ),이영종 ( Young Jong Lee ) 대한본초학회 2008 大韓本草學會誌 Vol.23 No.3