Platycodon grandiflorum root-derived saponins attenuate atopic dermatitis-like skin lesions via suppression of NF-kB and STAT1 and activation of Nrf2/ARE-mediated heme oxygenase-1

( Jae Ho Choi ),( Sun Woo Jin ),( Eun Hee Han ),( Bong Hwan Park ),( Hyung Gyun Kim ),( Tilak Khanal ),( Yong Pil Hwang ),( Minh Truong Do ),( Hyun Sun Lee ),( Young Chul Chung ),( Hee Suk Kim ),( Tae 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Purpose: The consequences of precipitously rising allergic skin inflammation rates worldwide have accelerated the risk of atopic dermatitis (AD). Natural product-based agents with good efficacy and low risk of side effects offer promising prevention and treatment strategies for inflammation-related diseases. We have already reported that Platycodon grandiflorum root-derived saponins (Changkil saponins, CKS) have many pharmacological effects, including anti-inflammatory and anti-allergic effects, but its influence on AD remains unclear. Therefore, we evaluated the inhibitory effect of CKS, mainly platycodin D, on AD-like skin symptoms in mice and the possible mechanisms in cells. Methods: Mice were sensitized and challenged with 2,4-dinitrochlorobenzene (DNCB). Four weeks after challenge, mice were treated with oral administration of CKS for 4 weeks. In addition, cells were used to evaluate the effect of CKS, mainly platycodin D, on the TARC expression regulated mechanism. Results: CKS attenuated DNCB-induced dermatitis severity, serum levels of IgE and TARC, and mRNA expression of TARC, TNF-α, IFN-γ, IL-4, IL-5, and IL-13 in mice. Histopathological examination showed reduced thickness of the epidermis/dermis and dermal infiltration of inflammatory cells and mast cells in the ears. Moreover, CKS and platycodin D inhibited TNF-α/IFN-γ-induced TARC expression through the suppression of NF-κB and STAT1 and induction of Nrf2/ARE-mediated hemeoxygenase-1 (HO-1) expression in cells. Conclusion: We suggest that CKS and platycodin D inhibited the development of AD-like skin symptoms by regulating cytokine mediators and may be an effective alternative therapy for AD-like skin symptoms.ⓒ2014 Elsevler Gmbh. All rights reserved.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

Stain improvement in the white button mushroom ‘Seolgang’ and its varietal characteristics in Agaricus bisporus

Byung-Joo Lee,Mi-Ae Lee,Yong-Gyun Kim,Kwang-Won Lee,Yong-Pyo Lim,Byung-Eui Lee,Ho-Yeon Song 한국버섯학회 2012 한국버섯학회지 Vol.10 No.4

The button mushroom (Agaricus bisporus) is one of the most widely cultivated important edible mushroom species. In the breeding of new button mushroom, ‘Seolgang’ was developed by crossing two monokaryons ‘CM020913-27’ and ‘SSU423-31’. Because of the secondarily homothallism, only a small percentage of the basidia produce 3 or 4 spores, which are mostly haploid (n) and do not fruit. Single spore cultures derived from these types of spores produce a vegetative mycelium that also contain a variable number of genetically identical nuclei per cell called monokaryon. The lack of clamp connections between monokaryon and dikaryon required a series of mycelial culture and fruiting test. After crossing, hybrids were cultivated on a small scale and on a commercial scale at a farm. For this, the spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of ‘Seolgang’ on CDA was better at 20℃ and 25℃ when it was compared with that of ‘505 Ho’. The mature cap shape of new strain ‘Seolgang’ is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 41.2 mm on average. In comparison with white strain ‘505 Ho’, the strain had a yield that was 9% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 19% firmer with a good shelf life. Days of fruiting body were 3-4 days later than those of ‘505 Ho’. The physical characteristics such as elasticity, chewiness, adhesiveness were better than that of ‘505 Ho’. Genetic analysis of the new strain ‘Seolgang’ showed different profiles compared to ‘505 Ho’, CM02913-27, SSU413-31, when RAPD primers A02 and O04 were used.

이소성 타액선에 의한 경부 누공 1예

김병균,김문범,이재봉,오창근,장호선,권경술 대한피부과학회 2003 대한피부과학회지 Vol.41 No.3

Ectopic salivary tissue is an uncommon etiology of a neck mass in an infant. It is due to anomalous embryologic development of salivary tissue. We have experienced a case of cervical fistula due to ectopic salivary gland in 3-year-old male. The discharge is saliva-like and related to meals. Histopathologically, mucinous acini are located in lower dermis and subcutaneous fat. When a cystic neck mass or fistula is present on cervical area especially in children, an ectopic salivary gland should be considered as one of the causes. (Korean J Dermatol 2003;41(3) : 394∼396)

財閥對策에 관한 硏究

金昊均 明知大學校 經濟硏究所 1994 경영연구 Vol.11 No.1

Sliding Mdde Control을 이용한 대체에너지용 연계형 인버터 시스템

안호균,김태엽 國立 昌原大學校 産業技術硏究所 1996 産技硏論文集 Vol.10 No.-

This paper proposes the instantaneous control method of a utility interactive inverter with unknown R-L series load. Sliding mode control and adaptive parameter estimation are applied to this system, which realize the following funtion: (1) synchronization of phase, frequency, and amplitude between inverter voltage and utility voltage at stand-alone mode. (2) phase synchronization between inverter current and utility voltage at interactive mode. A DSP-based(TMS320C31)PWM inverter controller is implemented to verify the effectiveness of the proposed control scheme. Simulation and experiment based on the secomd order model are done and the results show good dynamic response according to each mode and low THD.

Modified Pulse pattern을 이용한 대체에너지용 인버터의 순시제어

안호균,허재영 國立 昌原大學校 産業技術硏究所 1995 産技硏論文集 Vol.9 No.-

This paper proposes a modified deadbeat controlled-PWM inverter system for the alternative energy. The proposed PWM system utilizes the instantaneous and real time control scheme. Two-pulse PWM switching pattern is used to increase the fundamental component of the output voltage. It was verified through simulation and experiments that the proposed control scheme provided an excellent transient response, increased output voltage amplitude and accurate referance waveform tracking. In this paper, proposed algorithm is computed by the DSP(Digital Signal Processor: TMS320C25) and realized by IGBTs for the high frequency switching. Also PWM generating circuit is simplified by replacing hardware with software.

제조물책임비용을 고려한 신뢰도 최적설계 모형

김호균,배창옥 東義大學校産業技術開發硏究所 2003 産業技術硏究誌 Vol.17 No.-

Product liability (PL) law has been in force since July 2002 in Korea. PL burdens manufacturer with additiuonal cost and this PL cost depends on product reliability. Product reliability is influenced by the decisions made during the design and manufacturing of the product. In this paper, PL cost considering injury intensity, customer claim and product reliability is quantified and a reliability design model is presented. The optimal reliability and development period to minimize total cost which includes manufacturing cost, PL cost and product development cost are determined. Numerical experiments are illustrated to evaluate the model and sensitivity analysis is performed.

國家競爭力 강화를 위한 勞使關係 發展戰略

김호균 한국노동경제학회 2003 勞動經濟論集 Vol.26 No.2

韓國經濟의 知識經濟化를 촉진하여 국가경쟁력을 강화하기 위해서는 새로운 勞使關係가 확립되어야 한다. 과거 대량생산경제에서는 혁신의 기조가 노동을 실물자산으로 대체하는 것이었기 때문에 노사간의 이해대립은 물론 혁신에 대한 노동자의 저항도 불가피한 것이었다. 그러나 知識經濟p서는 知識勞動과 實物資産이 서로 보완관계에 있을 뿐만 아니라 교육과 숙련에 대한 투자는 收穫遞增을 보이고 있으므로 知識經濟에서는 知識과 숙련을 증진시키는 데 노사가 공동의 이해관계를 갖게 된다. 그러므로 知識經濟에서는 協力的 勞使關係가 가치창출을 극대화하기 위해서 필수적이다. 한국에서 協力的 勞使關係를 정착시키기 위해서는 '勞使政委員會'의 적극적인 역할이 필요하다. In order to enhance the national competitiveness of the korean knowledge economy it is necessary to establish new, cooperative industrial relations. In the past mass production econ omy the technological innovations generally substitute the real assets for the labour so that the interest conflict between employers and employees was inherent and the resistance of the labourers to innovations was unavoidable. In the knowledg e economy, however, the real assets and the labour are complementa ry to each other and the investments in skills and education bring increasing returns. These are the most important reasons for the common interest of employ ers and employees in improved skills and advanced education. In the knowledg e economy, therefore, establishing and maintaining the cooperative industr ial relations is a necessary condition to maximize the value creation, where in Kor ea the 'Tripartite Commission' can and has to play a active role.

자궁내막에 발생한 점막 연관 림프조직 (MALT) 림프종 1예

전균호,조현진,박성균,김천복,김대연,김종혁,김용만,김영탁,목정은,남주현 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.4

Non-Hodgkin's 림프종의 약 40%는 결절외(extranodal) 림프종으로 대개 위장관에서 발생하며 이들 중 점막 연관 림프조직(mucosa-associated lymphoid tissue: MALT)에서 기원한 림프종은 MALT 림프종으로 분류된다. 위 외의 부위에서 원발성으로 발생하는 MALT 림프종은 매우 드물지만 실제 거의 모든 신체 장기에 발생하는 것으로 알려져 있는데, 가장 흔한 부위인 위 외에도 폐, 갑상선, 침샘, 눈물샘에 발생하고, 드물게 안구, 유방, 방광, 신장, 흉선 등에도 보고된 바 있다. MALT 림프종은 오랜 기간동안 원격 전이하지 않고 국소 병변으로 남아 있는 특징이 있어 치료 방향 또한 국소 병변의 치료에 집중되며, 예후도 림프절 기원의 림프종에 비해 양호한 것으로 알려져 있다. 본 저자 등은 자궁 내막에 원발성으로 발생한 MALT 림프종 1예를 경험하였기에 문헌 고찰과 함께 이를 보고하고자 한다. Mucosa associated lymphoid tissue (MALT) lymphoma can rarely develop primarily in extranodal sites other than stomach which is the most common site for it. Other rare primary sites are small intestine, colorectum, esophagus, lung, thyroid, salivary gland, lacrymal gland, breast and skin. MALT lymphoma represents a distinct clinicopathologic features: it is usually localized to their original site for a long time and shows much more favorable prognosis than lymphoma at other site, but some MALT lymphoma can arise simultaneously or successively in different organ or give rise to another MALT lymphoma of other organ and can be multifocally disseminated or recurred. We report a very rare case of high grade B-cell lymphoma of mucosa associated lymphoid tissue (MALT) of the uterine endometrium, which was confirmed by immunohistochemical assay.