아세틸 콜린 유발 검사시 허혈성 심전도 변화와 흉통의 발생이 내피세포 기능장애에 미치는 영향

최철웅(Cheol Ung Choi),나승운(Seung-Woon Rha),김선원(Sun Won Kim),나진오(Jin Oh Na),임홍의(Hong Euy Lim),김진원(Jin Won Kim),김응주(Eung Ju Kim),한성우(Seong Woo Han),박창규(Chang Gyu Park),서홍석(Hong Seog Seo),오동주(Dong Joo Oh) 대한임상노인의학회 2010 대한임상노인의학회지 Vol.11 No.4

연구배경: 아세틸 콜린 유발 검사시 발생하는 흉통과 허혈성 심전도 변화가 아세틸 콜린에 의해 발생하는 관상동맥의 내피세포 기능에 어떤 영향을 주는지 알아보고자 했다. 방법: 흉통으로 내원하여 관상동맥 조영술과 아세틸 콜린 유발검사를 시행받은 1,085명의 환자를 대상으로 하였다. 아세틸 콜린 유발 검사를 하는 동안 허혈성 심전도 변화 및 흉통의 발생 유무에 따른 관상동맥 내피세포 기능의 특징을 비교하였다. 결과: 539명의 환자에서 아세틸 콜린 검사시 내피세포 기능 장애가 발생 하였다. 허혈성 심전도 변화가 있었던 그룹이 심전도 변화가 없었던 그룹보다 흉통이 더 많았고(78.1% vs. 60.8%, P=0.007), 관상동맥 조영술상 다발성 관상동맥 경련이 빈번하였고(59.4% vs. 40.6%, P=0.004), 관상동맥의 경련 정도도 더 심하였다(64.1% vs. 46.5%, P=0.006). 흉통이 있었던 그룹이 흉통이 없었던 그룹보다 허혈성 심전도 변화의 빈도가 더 높았다(14.7% vs. 7.5%, P=0.007). 또한 흉통이 있었던 그룹이 관상동맥 경련이 다발성으로 발생하는 경우가 많았고 (50.7% vs. 29.5%, P<0.001), 미만성으로 발생하는 경우가 많았고(87.1% vs. 75%, P<0.001), 관상동맥의 경련의 정도도 더 심하였다(51.6% vs. 43.5%, P=0.041). 결론: 아세틸 콜린 유발검사시 발생하는 허혈성 심전도 변화와 흉통은 관상동맥 경련의 정도와 경련이 발생하는 관상동맥 개수와 연관이 있다. Background: ECG changes and chest pain during acetylcholine (Ach) provocation tests may constitute important clinical parameters for Ach-induced endothelial dysfunction. We investigated the association between ischemic ECG changes and chest pain during the Ach-provocation test and angiographic characteristics of Ach-induced endothelial dysfunction. Methods: A total of 1,085 patients with anginal symptoms underwent diagnostic coronary angiography (CAG) and Ach-provocation tests. We compared angiographic characteristics of Ach-induced endothelial dysfunction according to the presence of ECG change and chest pain. Results: A total of 539 patients experienced Ach-induced endothelial dysfunction. Patients who experienced ECG change group suffered more frequent chest pain (78.1% vs. 60.8%, P=0.007), angiographically more frequent multiple coronary artery spasm (59.4% vs. 40.6%, P=0.004), and more severe coronary artery spasm (64.1% vs. 46.5%, P=0.006) than patients without ECG change. However, there was no difference in the length of endothelial dysfunction between the groups. The incidence of ischemic ECG changes in patients with chest pain was higher than in patients without chest pain (14.7% vs. 7.5%, P=0.007). Patients who experienced chest pain more often experienced multiple (50.7% vs. 29.5%, P<0.001), diffuse (87.1% vs. 75%, P<0.001), and severe coronary artery spasm (51.6% vs. 43.5%, P=0.041) than patients without chest pain. Conclusion: Ischemic ECG changes and chest pain during the Ach-provocation test are associated with multi-vessel involvement and severe coronary artery spasm. Chest pain is associated with the length of endothelial dysfunction, but ischemic ECG change is not.

A Comparative Proteomics Survey of Proteins Responsive to Phosphorous Starvation in Roots of Hydroponically-grown Rice Seedlings

Kim, Sang-Gon,Wang, Yiming,Lee, Chang-Hoon,Mun, Bong-Gyu,Kim, Pil-Joo,Lee, Sang-Yeol,Kim, Yong-Chul,Kang, Kyu-Young,Rakwal, Randeep,Agrawal, Ganesh Kumar,Kim, Sun-Tae The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.5

Rice takes up phosphorous (P) as major nutrient source for its growth and development when grown under anaerobic water-logged soil conditions. To better understand the underlying mechanisms and to develop potential protein biomarkers of P-starvation, hydroponically-grown rice seedlings in the complete media and phosphorus absence (P-starvation) of phosphorous nutrient solutions were investigated for physiological and proteome changes. The P-starvation manifested significant reduction in root growth in three-week-old seedlings compared to respective complete media. Furthermore, P-starvation also showed increased activity of acid phosphatase in roots of one- and three-week-old seedlings, suggesting that experimental design is suitable for proteomics survey of P-starvation responsive proteins. Two-dimensional gel electrophoresis coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis of total root protein from three-week-old seedlings identified 10 P-starvation responsive protein spots out of 140 high-quality protein spots. Identified 10 proteins were involved in metabolism and defense/stress response. Out of 10, 2 and 8 protein spots were found to be up- and down-regulated, respectively. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis of corresponding genes of four randomly selected proteins, including putative glyceraldehydes-3-phophate dehydrogenase (G3PDH, spot R1), S-adenosyl-L-methionine synthetase (SAMS, spot R4), ATP synthase subunit alpha (spot R6), and root-specific pathogenesis-related protein 10 (PR-10, spot R8), showed that just as protein abundance, these proteins are also regulated at the transcript level. Results suggest identified P-starvation responsive proteins are involved in maintaining nutrient homeostasis and/or associated with changes in root physiology under the absence of P.

무증상 갑상선기능저하증 환자에서 Thyroxine 치료에 따른 혈청 지질 및 아포지단백의 변화

조보연,신찬수,김원배,고창순,김성연,박혜영,박건상,이홍규,박형규,김숙경 대한내분비학회 1996 Endocrinology and metabolism Vol.11 No.1

Background: Subclinical hypothyroidism(SCH) is a common biochemical abnormality which can be found in routine screening tests of thyroid function. We are increasingly faced with the question of whether its an indication for thyroxine replacement therapy. The effect of thyroxine replacement on lipid profile in SCH has aroused a great interest because of an association of overt hypothyroidism(OVH) with hyperlipidemia and increased risk of coronary artery disease. Method: We prospectively evaluated the changes in lipids and apoproteins before and after thyroxine replacement therapy in 23 patients with SCH and in 37 patients with OVH. We measured serum total cholesterol and triglyceride using autoanalyzer, high density lipoprotein(HDL) chole-sterol by dextran sulfate method, Apo A1 and Apo B by immunonephelometric assay. Results: Thyroxine replacement therapy significantly decreased total cholesterol, low density lipoprotein(LDL) cholesterol and apo B levels, but did not affect the level of triglyceride, HDL cholesterol or apo AI in patients with OVH. In SCH, thyroxine replacement therapy with the doses to normalize serum TSH concentrations also decreased significantly the level of cholesterol and LDL cholesterol albeit apo B levels did not change. Moreover, in most of patients with OVH (11 of 12) and in all of patients with SCH(5 of 5) who had had hyperchlesterolemia before treatment, thyroxine replament normalized their cholesterol and LDL cholesterol levels. Conclusion: In regard to the beneficial changes in blood lipid levels, patients with SCH should be treated, especially in cases who have other risk factors for the development of atherosclerosis. If thyroxine replacement only will reduce the incidence of coronary artery disease in SCH remains to be elucidated by long-term prospective studies(J Kor Soc Endocrinol 11:41-51, 1996).

日中時間 變動에 따른 白鼠의 生理變化에 對한 補肝湯 瀉肝湯의 影響에 關한 硏究

金成圭,洪茂昌,金完熙 慶熙大學校韓醫科大學韓醫學硏究所 1986 慶熙韓醫大論文集 Vol.9 No.-

A Comparative Proteomics Survey of Proteins Responsive to Phosphorous Starvation in Roots of Hydroponically-grown Rice Seedlings

( Sang Gon Kim ),( Yi Ming Wang ),( Chang Hoon Lee ),( Bong Gyu Mun ),( Pil Joo Kim ),( Sang Yeol Lee ),( Yong Chul Kim ),( Kyu Young Kang ),( Randeep Rakwal ),( Ganesh Kumar Agrawal ),( Sun Tae Kim ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.5

Rice takes up phosphorous (P) as major nutrient source for its growth and development when grown under anaerobic water-logged soil conditions. To better understand the underlying mechanisms and to develop potential protein biomarkers of P-starvation, hydroponically-grown rice seedlings in the complete media and phosphorus absence (P-starvation) of phosphorous nutrient solutions were investigated for physiological and proteome changes. The P-starvation manifested significant reduction in root growth in three-week-old seedlings compared to respective complete media. Furthermore, P-starvation also showed increased activity of acid phosphatase in roots of one- and three-week-old seedlings, suggesting that experimental design is suitable for proteomics survey of P-starvation responsive proteins. Two-dimensional gel electrophoresis coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis of total root protein from three-week-old seedlings identified 10 P-starvation responsive protein spots out of 140 high-quality protein spots. Identified 10 proteins were involved in metabolism and defense/stress response. Out of 10, 2 and 8 protein spots were found to be up and down-regulated, respectively. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis of corresponding genes of four randomly selected proteins, including putative glyceraldehydes-3-phophate dehydrogenase (G3PDH, spot R1), S-adenosyl-L-methionine synthetase (SAMS, spot R4), ATP synthase subunit alpha (spot R6), and root-specific pathogenesisrelated protein 10 (PR-10, spot R8), showed that just as protein abundance, these proteins are also regulated at the transcript level. Results suggest identified P-starvation responsive proteins are involved in maintaining nutrient homeostasis and/or associated with changes in root physiology under the absence of P.

農民技術受容에 影響을 주는 要因의 比較分析 : 關係機關과 情報傳達媒體를 中心으로

鄭址雄,崔敏浩,金性洙,徐圭善,李昶植 서울大學校 農科大學 1984 서울대농학연구지 Vol.9 No.2

The purpose of the study was to analyze the variables affecting farmers' adoption process of agricultural innovations in Korea. Specific objectives of the study were to ; (1) review the adoption process and the variables associated with this process, (2) investigate the rate of adoption of selected agricultural innovations in the fields of rice production, animal husbandary and horticulture, (3) analyze communication media and change agencies or institutions which affected the adoption of agricultural innovations, and (4) identify the relations of the farmers' adoption and communication exposure behavior. Data for the study were collected from 393 cluster sampled farmers out of selected 17 rural villages in Korea. Five graduate students and three faculty members of Seoul National University participated in conducting interview with pre-tested questionnairs. The data were analyzed by HP 3,000 computer in the College of Agriculture, Seoul National University. Major statistical techniques used for the study were frequency distribution, chi-square and discriminant analysis. Statistical significance was tested at .05 level. The major findings of the study were as follows : 1. Variables associated with the agricultural adoption process were classified into the following six categories ; (1) perceived characteristics of agricultural innovations, (2) personality and characteristics of adopters, (3) the patterns of communication media or channels, (4) efforts of change agents. (5) priorities in agricultural development policies, and (6) characteristics of social system. 2. Rats of adoption were about 83% in application of silicate fertilizers on rice seed bed, 41% in feeding calf starter known as artificial milk to calves, 56% vinyl mulching practices in vegetable cultivation, and 67% in spreading raw rice straw on paddy land to improve soil conditions. 3. Time lag between awareness and adoption appeared to be about seven months in application of silicate fertilizers on rice seed bed, and about a year in feeding calf starter. 4. Change agent interpersonal communication revealed to be the most important factor affecting farmers' awareness in application of silicate fertilizers on rice seed bed, in feeding calf starter, and in spreading raw rice straw on paddy land, while localite interpersonal communication in vinyl mulching practices in vegetable cultivation. 5. Change agent interpersonal communication appeared to be the most important factor affecting farmers' adoption in application of silicate fertilizers on rice seed bed and in feeding calf-starter, Localite interpersonal communications were more important in vinyl mulching practices of vegetable cultivation and in spreading raw rice straw. Localite interpersonal communications were more important factor at adoption stage than at awarenss stage. 6. Agricultural extension agencies appeared to be the most important factor affecting farmers' adoption in application of silicate fertilizers on rice seed bed, in feeding calf starter, and in vinyl mulching practices. Based on the results of this study, the following are recommended for further development of agricultural extension services and effective adoption process of agricultural innovation. 1. The variables associated with the agricultural innovation decision process should be taken into consideration to improve effectiveness of change agencies or agricultural extension services. 2. Effective use of communication media along with the feedback messages from the change agency as well as client system be considered to increase effectiveness and meaningfulness of both systems. 3. Innovative agricultural research be continually sharpen its focus on the highest priority needs of client system by utilizing feedback from farmers through the communication link of change agents. 4. A long-term educational approach by using various communication media be emphasized, and regular field survey on innovation-decision process will prevent discontinuance of innovations.

신축학교 실내공기질이 초등학생들의 인지기능에 미치는 영향

김태섭,김선주,박시영,전만중,김규태,김창윤,정종학,백성옥,사공준 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.1

목적: 신축초등학교와 대조학교를 선정하여 교실 내 포름알데히드와 TVOCs를 측정하고 컴퓨터 신경행동검사를 이용하여 실내공기 오염물질이 학생들의 인지기능에 미치는 영향을 평가하였다. 방법: 신축초등학교 5학년 71명을 실내공기 오염물질 노출군으로 하고, 개교한지 12년째인 대구시의 일개 초등학교 5학년 2학급 63명을 대조군으로 하여 실내공기 오염물질농도 측정 및 컴퓨터 신경행동검사를 실시하였다. 결과: 신축학교에서 의도적으로 창문을 개방한 학급에서 1교시 포름알데히드 농도는 34.6 ㎍/㎥, 4교시에는 27.3 ㎍/㎥, 창문을 개방하지 않은 학급에서 1교시 80.2 ㎍/㎥, 4교시 127.1 ㎍/㎥, 대조학교에서는 2학급 각각 1교시에 12.6 ㎍/㎥, 7.5 ㎍/㎥, 4교시에 19.8 ㎍/㎥, 25.2 ㎍/㎥였다. TVOCs의 경우 신축학교의 의도적으로 창문을 개방한 학급에서 1교시에 농도는 487.2 ㎍/㎥, 4교시에 428.2 ㎍/㎥, 창문을 개방하지 않은 학급에서 1 교시에 농도는 1,283.5 ㎍/㎥, 4교시에 1,715.4 ㎍/㎥, 대조학교의 경우 2학급에서 각각 1교시에 농도는 240.9 ㎍/㎥, 150.8 ㎍/㎥, 4교시에 농도는 371.9 ㎍/㎥, 448.0 ㎍/㎥였다. 부호숫자 짝짓기의 평균반응시간은 대조학급이 1교시에 2,585 msec에서 4교시에 2,459 msec으로 감소한 반면 신축학교의 창문을 의도적으로 개방한 학급은 2,343 msec에서 2,510 msec으로, 평소대로 수업한 학급은 2,340 msec에서 2,563 msec으로 반응시간이 각각 167 msec, 223 msec 증가하였다. 1교시의 부호숫자 짝짓기 의 반응시간을 100%로 했을 때 창문을 의도적으로 개방 한 학급은 4교시에 5.9% 증가하였고(p<0.01), 평소대로 수업한 학급은8.3% 증가하였다(p<0.01). 반면에 대조학급은 4교시에 5.0% 감소하였다(p<0.01). 결론: 향후 학교의 실내공기질의 안전성 평가는 샘플링 된 공기의 오염수준의 측정뿐만 아니라 학생들의 건강영향도 함께 고려되는 것이 바람직할 것이다. Objectives: Most studies on the indoor air quality of newly built schools have focused only on the number of schools exceeding a standard or on subjective symptoms, such as sneezing, irritated eyes and an irritated nose. However, there has been no report on how poor indoor air quality affects children's academic performance. This study evaluated the effects of indoor air quality on the neurobehavioral performance of elementary school children. Methods: This study measured the indoor air pollutants and compared children's performance, using a computerized neurobehavioral test, between a newly built and a 12-year-old school. In addition, a questionnaire was administered regarding the indoor air quality related symptoms of children in a newly built and a 12-year-old school. Results: In the newly built school, the formaldehyde levels in the 1^(st) and 4^(th) classes with an open window were 34.6 and 27.3 μg/㎥, respectively. The levels in the 1^(st) and 4^(th) classes with the window closed were 80.2 and 127.1 μg/㎥, respectively. In the 12-year-old school, the formaldehyde levels in 1^(st) and 4^(th) class with open and closed window were 12.6 and 7.5 μg/㎥, and 19.8 and 25.2 μg/㎥, respectively. With respect to total volatile organic compounds, the levels in the 1^(st) and 4^(th) classes with the window open in the newly built school were 87.2 and 428.2 μg/㎥, respectively. In the 1^(st) and 4^(th) classes with the window closed, the total volatile organic compounds were 1,283.5 and 1,715.4 μg/㎥, respectively. In the 12-year-old school, the total volatile organic compounds were 240.9 and 150.8 μg/㎥, and 371.9 and 448.0 μg/㎥ in the 1^(st) and 4^(th) classes, respectively. In the case of a newly built school, the performance of the students in the neurobehavioral test in the 4^(th) class with the window closed was 8.3% lower than for those in the 1^(st) class with the window closed, but was only 5.9% lower when the windows were open. There was a statistical significant difference in the total numbers of symptoms between the two schools. Conclusions: These results suggest a proper evaluation of the indoor air quality within schools is required into both the level of contamination and the health effects of these contaminants toward children.

A Longitudinal Study on the Prevalence of Major Adult Chronic Disease in Korean Employees between 2001 and 2010

( Gyu-jang Lee ),( Soon-ki Kim ),( Hye-young Kim ),( Chang-kyou Lee ),( Seung-gwan Lee ),( Kyung-jin Cho ) 대한임상검사과학회 2012 대한임상검사과학회지(KJCLS) Vol.44 No.1

The Korean GDP per capita doubled during the last 10 years. As the GDP increases, Korean health behaviors such as, eating, drinking, smoking, and exercise habits changed as well. Compared to the remarkably advanced diagnostic technologies and therapeutics during the last decade, the leading causes of death in Korea scarcely changed. Among the leading causes of death, cerebrovascular disease, heart disease, diabetes, liver disease and hypertension are closely related to health behaviors. Using the database of medical check-up between 2001 and 2010, the authors performed a longitudinal study for the prevalence rates and the trends of major chronic adult diseases among the 2,721 workers along with their health behavior. Among the diagnostic test items, fasting blood sugar, cholesterol and BMI levels showed steady increase and these three test items all showed linear trends (p<0.001). The average blood sugar and cholesterol levels were near worrying levels. Moreover, the average BMI of males was foundto be at the overweight level, intimating the possibility reaching thee Obese Class I level soon if there are no aggressive counter measures. Fasting blood sugar and cholesterol levels were higher in a drinking group as compared to a non-drinking group, and the BMI levels were also higher in the drinking group and the smoking group as compared to the non-drinking and the non-smoking group (p<0.001). Finally, the authors believe that the fasting blood sugar, cholesterol and BMI levels should be designated as target monitoring test items in the medical check-up for Korean employees.

Label-free DNA detection with a nanogap embedded complementary metal oxide semiconductor

Kim, Chang-Hoon,Jung, Cheulhee,Lee, Kyung-Bok,Park, Hyun Gyu,Choi, Yang-Kyu IOP Pub 2011 Nanotechnology Vol.22 No.13

<P>A nanogap embedded complementary metal oxide semiconductor (NeCMOS) is demonstrated as a proof-of-concept for label-free detection of DNA sequence. When a partially carved nanogap between a gate and a silicon channel is filled with charged biomolecules, the gate dielectric constant and charges are changed. When the gate oxide thickness reduces, the threshold voltage is significantly affected by a change of the charges, whereas it is scarcely influenced by a change of the dielectric constant. In the case of DNA, those two factors act on the threshold voltage oppositely in an n-channel NeCMOS but collaboratively in a p-channel NeCMOS because of the negative charges of DNA. Hence, a p-channel NeCMOS with a thin gate oxide is more attractive for DNA detection because it enhances the shift of threshold voltage; that is, it improves the sensitivity of DNA detection. In addition, the shift of threshold voltage according to the nanogap length is also investigated and the longer nanogap shows more shift of the threshold voltage.</P>

신경섬유종증-제 2형의 진단과 치료 : 16례의 임상경험 Experience of 16 Cases

김정은,백선하,김종수,이상형,오창완,김동규,정희원,김현집,조병규,한대희,최길수 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.12

Neurofibromatosis type 2(NF-2) is a dominantly inherited disorder characterized by the occurrence of bilateral acoustic neurinomas and the frequent association of other central nervous system tumors. We present a retrospective review of 16 patients with NF-2 who were treated at our hospital from 1984 to 1995 in 13 cases, the diagnoses of Nf-2 were based on the criteria developed at the Consensus Development Conference of National Institute of Health in the United States, and in another 3 cases. the criterias of NF-2 were not fully satisfied, but the diagnoses of NF-2 were highly suspected. The average age of the patients was 27.6 years, ranging from 13 years to 56 years. The most common symptom was hearing difficulty : intervals between symptom onset and deafness ranged from 8 months to 6 years(mean : 2.9 years) One family of NF-2 was documented consisting of a sister a brother and their mother. Nine patients underwent operations on unilateral acoustic neurinomas : these were subtotally removed in eight patients and totally in one patient Among these patients five were deaf on the ipsilateral side at surgery. Among the other four patients with useful hearing before surgery, hearing was preserved to preoperative status in two patients. Four patients with diagnoses of meningioma, received operations to relieve mass effect with subtotal removal in two patients and total removal in the other two. Early diagnosis and treatment are the most important in the management planning of patients with NF-2 for reasons of early manifestation and rapid progression of the disease.