산악자전거 훈련이 크로스컨트리 스키 선수의 체력에 미치는 영향

윤병하,오수일 江原大學校 附設 體育科學硏究所 1998 江原大學校附設體育科學硏究所論文集 Vol.- No.22

The purpose of thins study is to examine the effect on physical fitness of MTB training in cross-country skier. The subjects are experiment-trained 10 students. and contrast 5 students. By analysing the recored of MTB training after 12-week-training. the results are follows: 1.In leg muscle strength. experiment group(63.44±22.43-89.44±25.88) was significantly higher than contrast group. 2.In abdominal muscle strength. experimental group(65.67±22.38-105.44±14.13) and contrast group(87.00±7.97-108.80±10.16) were increased significantly 3.In abdominal endurance muscle strength. experiment group(108.2±16.03-134.50±11.30) was significantly higher than contrast group.

배양된 래트의 임파구에서 펜타크로로페놀에 의한 hprt 유전자의 돌연변이율

윤병수 경기대학교 1996 論文集 Vol.38 No.2

래트의 혈액과 비장에서 T-세포를 분리 배양하여, 그 hprt(hypoxanthine phosphoribosyl transferase)유전자에서 pentachlorophenol(PCP)의 돌연변이효과를 측정하였다. 이 세포들은 dimethyl sulfoxide에 녹인 PCP를 최종농도 0-100 ppm되게 함유한 초기배양액에서 24시간 배양하여 돌연변이를 유도하였다. 처리된 세포들은 같은 시간 동안 concanavalin A와 interleukin 2에 의해 증식이 자극되었으며, 이 후 hprt 결핍 돌연변이 세포만을 분리하기 위하여 6-thioguanine이 함유된 배지에서 성장시켰다. PCP는 hprt유전자를 돌연변이 시키어, 50ppm의 PCP처리군은 무처리군에 비하여 약 5.5배의 돌연변이율을 높이는 효과를 보여 주었다. hprt유전자에 대한 50ppm의 PCP처리군은 그 돌연변이율이 3.74×10^(­6)로 나타났고, 반면에 대조군의 돌연변이율은 7.00×10^(-7)으로 측정되었다. The mutagenic potential of pentachlorophenol was measured at the hprt (hypoxanthine phosphoribosyl transferase) locus in Blood and splenic T-cells from rats. Cells were exposed for 24 hours at priming culture to 0-100 ppm (weigh/volume) pentachlorophenol in dimethyl sulfoxide. Treated cells were allowed at the same time to stimulate by concanavalin and Interleukin 2 and then seeded in medium containing 6-thioguanine to select for hprt mutants. Pentachlorphenol were mutagenic at hprt locus, with 50 ppm pentachlorophenol exhibiting activity at mutagenicity approximately 5.5-fold higher than background hprt- mutation frequency. At the hprt locus, an induced mutation frequency of 3.74×10^(-6) was produced by treatment with 50 ppm pentachlorophenol (Mutation frequency of control was 7.00×10^(-7)).

정신장애인 치료에 중요 역할 담당자의 정신질환에 대한 태도조사

김병후,정선욱,강연화,김윤,김창엽,홍진표,한오수,조맹제,김용익 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.5

Objectives : Attitudes of families of mentally ill, health personnels and community leaders to the mentally ill after the development and utilization of the community mental health program and reintegration of the chronically ill patients into society. This study was designed to compare the attitudes toward the mentally ill patient of the persons who have important role in treating the mentally ill and to find the variables influencing the attitudes. Methods : We measured attitudes toward mental illness in psychotic patients' families(N=139), community leaders(N=180) and health personnels(N=324) including mental health personnels before the implementation of community mental health services in Yonchon county and Seodaemun district area. The instrument used to measure attitudes was Opinion about Mental Illness(O. M. I.) scale. Results : 1) Health personnel showed lower Authoritarianism(Factor A) and higher Benevolence(Factor B) scores on the average than community leaders and psychotic patients families. 2) Current residence affect the attitudes in psychotic patients' families, not in community leaders and health personnels. Families in urban area showed lower Authoritarianism(Factor A) and Social Restrictiveness(Factor D) and higher Benevolence(Factor B) scores than in the rural areas. 3) Many patient variables were correlated to the attitudes of families. Duration of illness, number of recurrence, number of admission, age of patient and family burden were positively correlated mainly to Social Restrictiveness(Factor D). 4) Sex and religion of respondents showed no or weak relationship with the factor scores. Conclusions : Education for community leaders and families is required to achieve successful community mental health services. Community support program should be developed for chronic, recurrent and burdensome psychiatric patients in the first place.

흰쥐에서 paraquat독성에 미치는 tumor necrosis factor-α의 영향

차종희,박정수,양성훈,박재윤,이병래 조선대학교 2000 The Medical Journal of Chosun University Vol.25 No.2

Background : The toxicity of paraquat is due to the oxygen-derived radicals formed by the reaction of oxygen with bipyridylium radical cations. Tumor necrosis factor (TNF)-α induces the synthesis of mitochondrial manganese superoxide dismutase (Mn-SOD) and other protective proteins in a variety of tumor cells and these protein contribute to a protect tumor cells from free radical injury. In this study, the effects of TNF-α on paraquat-induced toxicity were investigated in rats. Methods : Experimental animal used was Sprague-Dawley male rat, body weight 150-200g. The paraquat toxicity was determined by survival rate. The SOD, catalse, and glutathione peroxidase(GSHPx) activity were spectrophtomerically determined by Crapo method, Aebi method and Flohe method, respectively. Results : The survival rate of paraquat-intoxicated rats was decreased by subctaneous injection of TNF-α. However, the survival rate of paraquat injected rats were increased by pretreatment of TNF-α, comparing to saline pretreatment. The liver SOD activity was increased significantly by TNF-α treatment, but kidney and lung enzyme was unaltered in TNF-α treated-rats, comparing to normal control rats. The increment of liver SOD activity by TNF-α treatment was dose- and time-dependant SOD activity of kidney and lung of rats were not changed by TNF-α injection. The activities of catalase and glutathione peroxidase and contents of thiobarbituric acid reactive substance(TBARS) in liver were not changed by TNF-α treatment. Conclusion : From these result, TNF-α potentiate paraquat toxicity in rats but TNF-α pretreatment reduce paraquat induced toxicity. Protective effect may be resulted from the increased liver SOD activities by TNF-α.

저염김치제조에 관한 기초적 연구 : 무우의 소금절이 과정중 소금의 침투와 칼슘의 용출 Movement of salt and calcium in the processing of salting

김순동,박병윤,강명수 효성여자대학교 새마을연구소 1986 새마을硏究論文集 Vol.6 No.-

The relation between salt penetration and elution of moisture,calcium during salting process of radish were investigated at differentsalt concentration as a basic research for the fermentation of lowsalted radish kimchi. And also, measured to the most desirable saltconrentration of kimchis from houses and department stores by sensoryassessment. Almong 20 kimchis, 8 kinds were selected by sensory assessment andtheir salt concentration were at range of 1.2~2.2%. The result mayinvolve increasing in preference for low salted kimchis. Moisture audcalcium of radish were lossed in proportion to increasing the saltconcentration. Desirable salting times were estimated to 2 hours at3%, 1 hour at 5% and 30 minutes at 10% salt concentration. Thetreatment of high concentration of salt shortened the salting time,but brought a great nutritional loss.

골수구성 백혈병 환자에게 발생한 결핵성 림프절염

이창섭,송진수,최평균,조재현,방지환,박경화,박완범,김홍빈,김남중,윤성수,박선양,김병국,오명돈,최강원 대한감염학회 2006 감염과 화학요법 Vol.38 No.5

혈액질환 가운데 결핵 발생이 증가한다고 알려진 것은 호지킨 림프종을 포함한 림프증식성질환 그리고 모양세포성 백혈병 등이 있다. 또한 동종 골수이식을 받은 환자에서도 결핵은 증가한다고 알려져있다. Kaplan 등은 골수구성 백혈병 환자에서 결핵 발생이 증가한다고 보고하였다. 그러나 골수구성 백혈병 환자에서 결핵의 발생이 증가하는 이유에 대해서는 아직까지 정확하게 밝혀지지 않았다. 저자들이 2년 동안 후향적으로 조사한 180명의 성인 골수구성 백혈병 환자들 가운데 결핵이 발생한 환자는 4명이었고, 발생 부위는 모두 림프절이었다. 결핵은 골수구성 백혈병의 특정 아형에만 국한되어 발생하지 않았지만, 50%에서 FAB 분류에 의한 M4였다. 림프절이 종대된 골수구성 백혈병 환자에서 특히, 결핵의 유병율이 높은 나라에서는 결핵성 림프절염도 감별진단에 포함시켜야 한다. During the neutropenic phase, leukemia patients receiving chemotherapy are prone to bacterial and, fungal infections; occasionally mycobacterial, viral and protozoal organisms may also cause infections. Mycobacterium tuberculosis infection was reported very rarely in these patients. This report describes four patients with M. tuberculosis infection identified from 185 adult patients who were diagnosed myelogenous leukemia between January 2003, and December 2004. There was no patient with M. tuberculosis infection from 44 lymphoid leukemia and 11 acute biphenotypic leukemia patients. Sites of infection were all lymph nodes. Three among four patients were presented with lymphadenopathy at initial diagnosis of leukemia, and the other one presented with lymphadenopathy after induction chemotherapy. There was no patient presented with lymphadenopathy during the neutropenic phase. Tuberculous lymphadenitis was presented in a patient with three acute myelogenous leukemia (FAB class 2 M4, 1 M2) and a chronic myelogenous leukemia, accelerated phase. An acute myelogenous leukemia patient had a leukemic cell and tubercle bacilli in the same lymph node. Tuberculosis should also be included as a differential diagnosis in myelogenous leukemia patient with lymphadenopathy, especially in the countries in which the disease is endemic.

버들치(Rhynchocypris oxycephalus)의 vitellogenin 생성량 측정을 이용한 수중 estrogen휴 화합물의 정량분석법에 관한 연구

김을환,이도부,한상훈,윤병수 경기대학교 부설 기초과학연구소 2005 경기대학교 기초과학논문집 Vol.18 No.1

Vitellogenin(Vtg), a phospholipoglycoprotein precursor of egg yolk, is synthesized and secreted from the liver in response to estrogens in female fish. Vtg is normally undetectable in the blood of male fish, but can be induced by exposure to chemicals possessing estrogenic activity. Thus, the presence of Vtg in blood of male fish can serve as a useful biomarker for assessing previous exposure to estrogenic compounds. In the present study, Vtg was abnormally expressed in Rhynchocypris oxycephalus treatment of Estradiol Benzoate (E_(2)). As the result, it was found that the level of Vtg in blood from R. oxycephalus was increased by treated quantity of E_(2) with dose-effect manner.

Chlamydia psittaci strain fransis의 plasmid pCpA1과 C. psittaci strain 6BC의 plasmid의 염기서열 상동성 분석

한상훈,정규회,G. V. Stokes,윤병수 경기대학교 1999 論文集 Vol.43 No.2

In Chlamydia, there is not efficient transformation vector system. This defect makes hard to study of Chlamydia. As a first process for E. coli-to-chlamydia shuttle vector construction, we executed DNA sequencing analysis of Chlamydia psittaci 6BC plasmid whole origine and parts of ORFs. We compared them with already known Chlamydia psittaci francis pCpA1 whole origine and parts of ORFs sequences using Computer DNA sequence analysis program PCGENE and demonstrated that partially analyzed origins and ORFs on two plasmids matched perfectly each other.

콘크리트 표면성능개선에 의한 중성화억제방안에 관한 실험적 연구

강석표,김경덕,이병기,길배수,홍성윤,김무한 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

Penetration of concrete by aggressive materials in solution may cause an undesirable effect on concrete durability, for instance when Ca(OH)₂ is being leached out or an attack by aggressive liquids takes place. In particular, carbonation occurs in concrete because the calcium bearing phases present are easily attacked by carbon dioxide and converted to calcium carbonate from surface of concrete, It has been known that the durability of reinforced concrete structure is concerned with the surface performance of concrete which affects on the carbonation occurred by invasion of CO₂ gas and the corrosion of steel bar occurred by O₂ In this paper, the effects of carbonation resistance by improvement of surface performance for concrete are investigated according to the accelerated carbonation test. It is believed that the carbonation resistance of concrete has been significantly improved by the application of penetrative hardening agent due to the formation of a dense surface.

Species-specific identification of various pathogens against honeybee by using PCR, real-time PCR and ultra-rapid PCR

Byoung-Su Yoon 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

The infectious pathogens against honeybee (Apis mellifera) comprise a heterogeneous group of bacterial, viral, and fungal organisms including Paenibacillus larvae, Deformed Wing Virus (DWV) and Nosema apis. Many species like Paenibacillus larvae, Deformed Wing Virus (DWV) and Nosema apis have been isolated from a number of different continents, e.g. America, Asia and Europe, indicating its wide spread in whole nature. Little is known about the occurrence and distribution in the environment of these pathogens. For a more rapid, systematic and efficient monitoring of each pathogenic species against honeybee in the environment, PCR-based detection systems were developed that allows species-specific identifications of various pathogenic species with one reaction. These could be achieved by selecting specific primers from conserved regions of each species with speciesspecific DNA sequence variations. For the detection of any already known pathogen, well-developed PCR-detection system allows the specific detection of expected pathogenic species based on its specific nucleic acid sequence. Since each pathogenic species delivers a specific PCR-product of different size, bands can be distinguished very easily by simple gel electrophoresis. After the development of real-time PCR system, PCR-based specific detections of honeybee pathogens were dramatically improved their applications, from just detection to quantification of pathogens. These systems, quantitative PCR (qPCR) for the detection of honeybee pathogens, could be distinguished from previous PCR detection on the points of “real-time”, “easy” and “quantitative”. Moreover, very rapid PCR, so-called “Ultra-Rapid Real-Time PCR” were developed recently in field of pathogen-detection. Typical Honeybee pathogens such as Paenibacillus larvae, Israelli acute paralysis virus (IAPV) were successfully detected inner 7 minutes using 30 cycled Ultrarapid PCR. According to development of more rapid apparatus, even 30 cycled, 1 minute PCR seems to be possible. Ultla-Rapid PCR was currently attempted to apply for the direct detection system of all viral pathogens against honeybee from bee-samples and different environmental probes.