제지 폐수의 질소·인산 동시 제거를 위한 생물학적 처리기술 개발

권기석,노윤숙 안동대학교 환경연구소 2001 환경연구 Vol.1 No.-

주변에서 원유로 오염된 토양과 난분해성 화합물을 포함하고 있는 폐수 등에서 균주를 분리하여 40여종의 분해균주 중 가장 분해력이 우수한 분해균주 4종을 LB(Luria Bertani)배지로 최종분리하였다. 자연계의 균원 시료로부터 분리된 균주 중 점성을 갖는 균주를 선별하여 그 중 표준 점토광물인 kaolin clay에 대한 응집활성이 뛰어난 34종류이 균주를 선택하였으며, 폐수 내에서 응집활성이 높은 응집균주 4종을 최종 분리하였다. 균주 #46(Bacillus circulans), O24(Pseudomonas sp.)2종을 제외한 나머지 균주들의 동정결과는 #24T3, #24P3, #24B3는 Bacillus sp.로 동정되었고 #81, #152, 29은 미동정 되었다. 분해균주의 경우 TOC가 84%, T-N이 98%, T-P가 68%의 처리효율을 보였다. 응집균주의 경우 TOC가 80%, T-N 98%, T-P가 71%의 처리효율을 보였다. 분해+응집균주가 microcosom을 형성한 경우 TOC가 85%, T-N의 경우 98%이상, T-P의 경우 70%을 동시에 처리할 수 있다. From the enrichment culture, 40 strains were isolated, Among them, four strains were screend as having significant biodegradation activity of crude oil and toxic compounds containing wastewater. Also, for the screening of the microorganims, 34 bacterial strains were selected. Among them, four strains were screened as producing the most excellent flocculating material. The strains were identified that 46 was Bacillus circulates, 024 was Pseudomonas sp., 24T3, 24B3, 24P3 were identified Bacillus sp., respectively, And 81, 151, 29 were non-identification. The optimum cultural conditions were as follows; Bioflocculant producing strains : Glucose 20.0g, NH_(4)NO_(3) 2.0g, K_(2)HPO_(4) 0.8g, KH_(2)PO_(4) 0.6g, MgSO_(4)·7H_(2)O 0.05g, MnSO_(4)·4H_(2)O 0.05g, Soytone 0.lg, Tryptone 0.lg Yeast extract 0.lg, CaCO_(3) 0.3g in 1 liter of distilled water, 30℃ for temperature and pH 7.0. Biodegrade strains : LB(Luria Bertani) medium, 30℃ for temperature and pH 6.8∼7.5. The removal rates of total nitrogen and total phosphate by bioflocculant producing strains against pulp wastewater were T-N 98% and T-P 71%, respectively. In the case of biodegrade strains shown that the removal rates were T-N 98% and T-P 68%, respectively. In the case of mixed biodegrade strains and bioflocculant producing strains shown that the removal rates were T-N 99% and T-P 76%, respectively. The strains will be apply simultaneously T-N 98% and T-P 70% from pulp waserwater.

제지폐수처리용 생물고분자응집제를 생산하는 Bacillus sp. K-111의 배양특성조사

권기석,손용호,최선택,정석관,송숙희,김동걸 7개 국립대학교 환경연구 논문집 공동발행 위원회 2002 환경연구논문집 Vol.2 No.1

Flocculant-producing microorganisms were screened from obtained strains in the laboratory using a pulp-wastewater treatment as the flocculating agent. K-111 strain that had high flocculating activity among them was selected and identified as Bacillus sp. K-111 16s rDNA sequencing. The favorable medium for the production of flocculant was glucose 1.5%, NH_4NO_3 0.2%, tryptone 0.01%, K_2HPO_4 0.08%, KH_2PO_4 0.06%, CaCO_3 0.03%, MgSO_4·7H_2O 0.005%, MnSO_4 0.005% in 1 liter of D.W. at initial pH 7.0. The optimum culture temperature and pH were 30℃ and pH 7.0, respectively. the flocculating activity was observed most highly after 36 to 48 hr of cultivation at the optimum conditions. the flocculating activity of produced biopolymer on optimum conditions was about 2.5-fold higher than that of screening medium.

忠州市 地域特性을 反暎한 共同住宅 計劃硏究 : 충주지역 거주자의 APT 선호특성을 중심으로

朴碩鉉,朴義權,柳顯紀,尹勝照,孫泰鎭,金基洙 충주대 2003 産業科學論文集 Vol.11 No.-

The purpose of this research is to analyze the correlation of the characteristics of dwellers and residential preference in apartment housing through the case study of 340 samples in 25 housing estates which were built in Chung-ju city The contents of this research consist of two main parts. The first part is to identify characteristics of dwellers, which might have an influence on residential preference in apartment housing. The second part is to identify the main physical elements of residential preference that can be influenced by the personal characteristics. Thus, this research will contribute to the establishment of the planning methods which increase residential preference in apartment housing, while promoting the quality of residential environment in Chung-ju city.

共同住宅 居住者 特性과 住居環境滿足度의 相關關係에 관한 硏究

金基洙,朴碩鉉,梁富弘,崔生吉,朴義權,孫泰鎭,成基文 충주대 2004 産業科學論文集 Vol.12 No.-

The purpose of this research was to analyze the correlation of the characteristics of dwellers and residential satisfaction in apartment housing. The contents of this research is to identify characteristics of dwellers, which might have an influence on residential satisfaction in apartment housing. In conclusion, the number of a family, the floor level, the size of housing unit and tenure had statistically significant correlations with the residential satisfaction in apartment housing.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

운전시간이 폐수처리에 미치는 영향

최충식,하청근,권기석 7개 국립대학교 환경연구 논문집 공동발행 위원회 2002 환경연구논문집 Vol.2 No.1

The aim of the thesis is to value the features of Sequencing Batch Reactor and to discover the optimum condition by using synthetic wastewater. So as to develop the economical, efficient wastewater treatment technology which can remove substrate and nitrogen compounds by SBR Fill and Reaction time control. The results are summarized as fallows. 1. COD and T-N removal efficiency was below 95% in case of volumetric organic loading of 3㎏ BOD/㎥ day by Sequencing Batch Reactor. 2. Almost COD removed in the Fill time. 3. Various DO concentration are maximum in the Reaction time, and minimum in the Settle and Idle time. 4. COD and T-N were almost removed in Fill and Reaction time, the most suitable period was 5-3-2hr(Fill-Reaction-Settle). Removal efficiency of COD and T-N were over 98%.

페놀분해 효모 Candida tropicalis PW-51의 분리 및 분해특성

김성빈,김치경,김희식,이창호,신기선,권시석,윤병대,오희목 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

본 연구는 페놀과 포름알데히드를 포함하는 페놀계수지 산업폐수의 생물학적 처리에 이용할 목적으로 포름알데히드의 존재하에서 페놀분해능이 우수한 효모를 sludge로부터 분리하여, 형태적 및 생리 생화학적 특징을 조사하여 Candida tropicalis PW-51로 동정하였다. 회분식 배양에서 C. tropicalis PW-51의 페놀 분해한계는 2,000 mg/ℓ이며, 58시간내에 완전히 분해하였다. C. tropicalis PW-51은 초기접종량이 9×10 exp (6) cells/㎖, 배양온도는 30℃, pH는 7.0에서 페놀 분해효율이 높았으며, 500~2,000 mg/ℓ의 페놀농도에서 조사된 페놀에 대한 평균 분해율은 페놀 1,000 mg/ℓ에서 45.5 mg/ℓ/h 가장 높았다. C. tropicalis PW-51의 페놀분해시 catechol 1,2-dioxygenase의 활성이 크게증가하므로 ortho-path-way에 의해 페놀을 분해하는 것으로 판단되었다. 따라서, C. tropicalis PW-51는 페놀계 수지 산업폐수의 생물학적 처리에 효과적으로 이용될 수 있는 균주로 사료된다. For the biological treatment of phenolic resin wastewater containing phenol and formaldehyde, a phenol-degrading yeast was isolated from the papermill sludge, and then identified as Candida tropicalis PW-51 according to morphological, physiological and biochemical properties. The strain was able to degrade high phenol concentrations up to 2,000 mg/ℓ within 58 hours in batch cultures. Phenol-degrading efficiency by the strain was maximum at the culture conditions of a final concentration of 9×10 exp (6) cells/㎖ 30℃ and pH 7.0. The mean degradation rate of phenol was highest at 45.5 mg/ℓ/h in 1,000 mg/ℓ phenol from 500 mg/ℓ to 2,000 mg/ℓ phenol. Because the enzyme activity of catechol 1,2-dioxygenase increased in the course of degradation of phenol, it seems that this strain degrades phenol via the ortho-cleavage of benzene ring. The isolate C. tropicalis PW-51 could be effectively used for the biological treatment of phenolic resin wastewater.

안동호 상류 운곡천의 이화학적 수질특성과 식물플랑크톤 군집 특성

이중복,이희무,이건주,박정원,박재충,김동걸,권기석 7개 국립대학교 환경연구 논문집 공동발행 위원회 2002 환경연구논문집 Vol.2 No.1

This investigation is about the characteristics of phytoplankton community and physciochemical water quality of specific 6-point the Woon-kog stream system in upsteam of the Andong Lake. DO value was showed over 8.1㎎/L at each site and COD_Mn, BOD, T-N, T-P tend to increase as they stream down and that the existence and dominance of phytoplankton was low and it was difficult to conclude the definite correlation of water quality and phytoplankton community. Finally, it seemed to be desirable that alternatives for pollutional reduction should be made and performed on the basis of the continuous monitoring of the inflow to preserve the Andong Lake.

[P6-59] Optimal culture condition for producing the red pigment by Monascus sp. MMK2

Gi-Seok Kwon,Ji-Won Shin,Chung-Sig Choi,Se-Won Lee,Jung-Bok Lee,Chun-Pyo Jeon 한국식품영양과학회 2005 한국식품영양과학회 학술대회발표집 Vol.2005 No.10

Biodegradation of Organochlorine pesticide, Endosulfan by isolated strain, Klebsiella oxytoca KE-8

Kwon Gi-Seok,Kim Jang-Eok,Kim Taek-Kyum,Shin Kee-Sun,Kim Dong-Geol 한국생명과학회 2002 한국생명과학회 심포지움 Vol.35 No.-