서울시에서 산발적으로 분리된 Salmonella enterica Serotype Typhi 임상분리균주들의 분자역학적 분석

이희무,김철현,손장욱,김민자,박미선,이복권 대한감염학회 2000 감염 Vol.32 No.5

Fusarium Graminearum이 Choline Esterase에 미치는 영향

李喜茂 慶北專門大學 (영주경상전문대학) 1983 慶北專門大學 論文集 Vol.4 No.-

Scabby barley collected in kyung Buk, Yung Poong Gun district fed to healthy 2 year cows, and detcermination of Choline-Esterase Activity in Blood of cows. The results obtained in this investigation are Summarized as follows. 1. Choline-Esterase Activity in the blood of cows fed scabby barley has beenn decreased. 2 The poisonous component of the Scabby barley thought to be Anticholinesterase and hyocy-amine, atropin, Scopolamine.

한국산 노랑초파리 자연집단의 Hybrid Dysgenesis에 관한 연구 : P factor의 분포 및 P-M system에서의 type conversion

이희무,허미경 안동대학교 기초과학연구소 1990 基礎科學 硏究論文集 Vol.1 No.1

Gonadal dysgenesis (GD) sterility associated with P-M system in Drosophila melanogaster from Cheju, Sokch'o and Pusan were examined in order to determine the distribution of the chromosomal P factors and the extrachromosomal cytotypes. And type conversion among the three types, P, Q and M, of the P-M system were investigated. True P elements for potential GD sterility were practically null or nearly absent in these populations. The few exceptional males showed very weak P activity of 10-20% F1 female sterility on crossing with Canton-S females. Most of the isofemale lines from the three populations showing intermediate and different levels of GD sterility. This suggests the existence of P factors and cytotype polymorphism in these populations. 61-69% of the isofemale lines from Cheju and Sokch'o populations were typical Q strain, and most of the remaining lines were M' type. But M' type is predominant in Pusan population. Among 16 isofemale lines determined the P, Q, M' and M types at the time of the first generation, about 69% (11/16)of the lines have shown type conversions during the laboratory culture for 15 generations, and 5 lines (31%) have retained their original types. These type change were P→Q, Q→M', M-M' and M'→Q. Changes from the other types to the P type were never found.

가두리 양식장이 안동댐 수질오염에 미치는 영향

이희무 安東大學 1994 安東大學 論文集 Vol.16 No.1

영남권 일대의 용수로서 중요한 위치를 차지하고 있는 안동댐의 가두리 양식장 수역의 미생물 오염과 이들의 생화학적, 생리적 특성은 1994년 4월, 6월, 8월 3회에 걸쳐 13개 정점에서 조사, 연구하였다. 우점종을 나타낸 13개 균속, 18개 종은 Enterobacteriaceae과의 Citrobacter diversus, C. freundii, Klebsiella axytoca, K.terrigena, Enterobacter. Cloacae, E. agglomerance, Erwinia herbicola, Serratia plymuthica, Hafnia alvei. Vibrionaceae과의 Aeromonas hydropyila. Micrococcaceae과의 Staphylococcus xylosus. 타속의 Bacillus subtilis, B.megaterium. Pseudomonadaceae과의 Pseudomonas aeruginosa, Pseudo. Putida. Neisseriaceae과의 Acinetobacter caleoaceticus. 타속의 Flavobacterium breve, Alcaligenes faecalis로 나타났으며, 안동댐 수질의 미생물 오염중 대장균과 분원성 대장균 분포가 높았다. The bacterial contamination and biochemical, physiological characteristics were studied in Andong Dam by artificial fishes cultivate, during April, June, August, 1994. The bacterial dominant species were 18 species of 13 genus. The results was obtain as follows: The species of the genus Citrobacter were C. diversus and C. freundii, klebsiella were K. oxytoca and K. terrigenu, Enterobacter were E. agglomerance, Erwiniaviere E. herbicola, Serratia were S. plymuthica, Hafnia were H. alvei of the Enterobacteriaceae. Aeromonas were A. hydrophila of the Vibrionaceae. Micrococcus were Staphylococcus xylosus of the Micrococcaceae. other genus were Bacillus subtilis and B. megaterium. Pseudomonas were P. aeruginosa and P.Pulida of the Pseuiomonadaceae. Other genus were Flavobacterium breve and Alcaligenes faecalis. The bacterial contamination of the water quality in Andong Dam showed higher coliform and fecal coliform.

Affect of Temperature and Gamma-Irradiation on Production of A flatoxin on Rice by Aspergillus flavus

李喜茂 慶北專門大學 (영주경상전문대학) 1984 慶北專門大學 論文集 Vol.5 No.-

Aspergillus flavus A-124G(ATCC 15517) 에 의한 쌀의 mycotoxin 생성에 대한 온도의 영향과 분생포자의 생존에 미치는 방사선조사와 가열의 영향을 검토하였다. Aflatoxin생성의 최적온도는 30℃였다. 수분함량이 9%, 온도 1O℃일때는 aflatoxin을 검출 할 수 없었고, 수분함량 15%에서는 미량이 생성되었다. 감마선과 가열처리에서 뚜렷한 상승영향을 나타내며 D_(10)값과 유도선량이 감소함을 보였다. The affect of temparature and gamma-irradiation,heat on the survival of conidia on mycotoxin-producing on rice by Aspergillus flavus A-124G(ATCC 15517) has been studied. The optimum temparature for producing of aflatoxin was 30℃. No aflatoxin was detected at the moisture levels of 9%. and at the temparature levels of lO℃, only traces at 15% moisture. Treatment of gamma- radiation and heat (10 minutes at 50℃) showed a synergistic effect, remarkable decreases in D_(10) values and induction doses in Asp. flavus

쌀의 油脂成分이 酸化過程에 미치는 영향

李喜茂 慶北專門大學 (영주경상전문대학) 1977 慶北專門大學 論文集 Vol.1 No.-

Hexane과 ethanol을 利用하여 米粒에서 油出한 쌀 油脂를 Folch's method에 依해 精製하고, 이들을 紫外線 照射 또는 40˚C 暗祈에 저장하여 쌀 抽脂의 酸化樣相을 살펴 본바, 다음과 같은 結論을 얻을 수 있었다. 1. 酸素吸收에 依한 重量增加現象은 紫外線 照射에 의하여 3日以內에 뚜렷한 重量增加 (0.6-0.8 以上)을 보였으나, 40˚C 暗所에서는 45日 以後에 비로서 현저한 重量增加의 商을 볼 수 있었다. 各處理別 誘導期間은 hexane 抽出油脂가 紫外線照射에서는 1日, 40 辯所에서는 30脚이있고, ethanol摘出油指는 紫外練照財예서는 25, 40˚C 暗所에서는 40日 이었으며, hexane抽出油脂가 ethanol抽出油脂보다 그 安定性이 多少 낮은 것으로 나타났다. 또한 各處理別 變敗臭는 誘導期間보다 약간늦게 볼 수 있었다. 2. 各處理別로 總 Carbonyl 化合物 및 malonaldehycle(M. A)의 변화를 살펴 본 바, 紫外線 照射지역에서는 Carbonyl價가 2-4 日째 M.A.는 7日頃에 그 生成量이 各各 頂點을 형성하다가 천천히 減少되었고, 40˚C 暗所에서는 Carbonyl價 및 M.A. 生成量 共히 55日까지 徐徐히 增加하였다. 3. 全般的으로 hexane抽出油脂가 ethanol抽出油脂보다 酸化反應이 더 빨랐으며 이는 脂肪酸組成 및 未確認된 抗酸化物質의 存在에 依한 可能性으로 생각된다. 그리고 紫外線 照射는 기지의 油脂의 酸化를 급격히 促進시킨다. 4. Hexane에 依해 抽出된 쌀油脂를 油出直後와 40˚C 55日間 저장처리한 후의 脂肪酸組成을 살펴 본 바 linoleic 및 linolenic acid가 뚜렷이 감소함을 볼 수 있고, 相對的으로 Oleic, Stearic, Palmitic acid 等이 증가하였다. Oxidative changes in the rice lipids under accelerated condition were studied by measurine the changes in weight gain, total carbonyl compound, malonaldehyde, and fatty acid composition. Rice lipids were prepared by extraction with either n-hexane or ethanol from polished rice grain and purified by Folch's method. The lipid preparations were either incubated in dark at 40˚C or irradiated with ultra-violet light for a period of 60 days. Weight gain by oxygen absorbed sharply increased within 3 days in the rice lipids under UV light irradiation. However, with the rice lipids at 40˚C incubation a moderate incrcase in weight was observed only after 45 days storage. Their induction periods were one day(hexane extracted, under UV light), 2 days(ethanol extracted, under UV light), 30days (hexane extracted, at 40˚C), and 40 days (ethanol extracted, at 40˚C) respectively. Oxidative rancid odor appeared at the end of the induction period. Total carbonyl compound and malonaldehyde markedly increased within 7 days, and decreased in the rice lipids under ultra violet light irradiation, while at 40˚C incubation they were continued to increase slowly throughout the storage. The hexane extracted lpid were less stable than ethanol extracted lipid on the basis of oxygen absorption, malonaldehyde and other carbonyl compound for-mation. With the hexane extracted lipid during 55days incubation at 40˚C, the contents of linoleic and linolenic acids decreased, while the oleic, Palmitic and stearic acids increased.

合成洗劑(음이온계 LAS)의 微生物的 分解

이희무,최선택 安東大學 1988 安東大學 論文集 Vol.10 No.1

LAS계의 합성세제가 유입되는 하상의 오니 및 토양으로부터 LAS분해능이 우수한 세균을 분리한 후 균학적 성질을 조사하여 Azotobacter chroococcum S41로 동정하였다. Azotobacter chroococcum S41은 탄소원으로서 대부분의 탄수화물을 잘 이용하였으며 특히 raffinose와 sucrose를 잘 이용하였다. LAS분해를 위한 질소원으로는 (NH2)2CO와 (NH4)2SO4를 가장 잘 이용하였다. LAS를 4,000ppm되게 첨가한 NB배지에서도 생육이 비교적 양호하였다. LAS분해를 위한 최적온도는 30℃, 초기 최적 PH는 6.0부근이었다. LAS를 첨가한 경우 분해율은 약 65%이었다. A bacterium which degrade linear alkylate sulfonate (LAS) was isolated from the sludge of industrial areas and identified as a strain of Azotobacter chrocooccum S41. The bacteria utilized well as a carbon source all carbohydrates used in this experiment, specially raffincse and sucrose. Alto the bacteria utilized (NH_(2))_(2)CO and (NH_(4))_(2)SO_(4) as a nitrogen source best for the bicdegradation of LAS. The optimum temperature and initial pH for the bicdegradation of LAS was 30℃ and 6.0 respectively. When the bacteria were cultured at 30℃ for 8 days in LAS medium containing LAS 40ppm, the bicdegradation rate of LAS was about 65%

腸毒素生産 大腸菌의 放射線照射와 熱處理에 미치는 영향

이희무 安東大學 1986 安東大學 論文集 Vol.8 No.1

Present work was conducted to investigate the effects of gamma-radiation and heat. Irradiated at doses of 10krad using indoor gamma room of approximately at 10,000ci of ^(60)Co and heat 10 minutes at 47℃, 52℃, their sterilizing effect was revealed differently depending on of treatments. Irradiation heating showed a synergistic effect wheareas Pre-irradiation heating revealed the opposite effect and the effect differed slightly with heating temperature.

임하댐의 위생학적 수질조사 연구 ( Study on the Hygenic Water Quality in Imha Dam )

이희무 대한환경위생공학회 1995 대한환경위생공학회지 Vol.10 No.1

우유 단백질 Casein의 알콜침전 시험에 관한 연구

이희무 건국대학교 1976 論文集 Vol.3 No.1

The alcohol precipitation test (APT) is widely used in the inspection of casein in masure milk, whereas. The APT in masure milk inspection is not specially known. The APT is used to determine the precipitating ability of milk by heat used in sterilization and evaporating process at the milk plant. The APT may also be used to detect abnormal milks such as acid milk colostrum, and any milk in which the salt balance is disturbed so that it may be more subject to precipitation than normal milk. In the experiments the applicability of the ATP of milk was studied. The results obtained by using several samples of milk are as follows : 1.As all the fresh samples (100%) were APT positive by using 70% ethanol which is used in the practice of milk and 3 put of several samples were positive by using 70% ethanol, it is suggested that 70% ethanol may be applied in the APT of manure milk. 2.The distribution of natural acidity (apparent acidity) was between 0.12% 0.30% and the amount of natural acidity did not significantly affect the precipitating ability of masure milk by APT. 3.The freshness of milk cannot be detected sharply by APT even though 70% ethanol is applied.