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This study was conducted to investigate the Microbial Antagonism in Plant Disease Control. In the Control of Griseofulvin, Cycloheximide and Streptocycline was carried out in various combination with strains of Xanthomonas citri and Helminthosporium Oryzae in order to observe the Synergistic action of these drugs. The results obtained wers Summarized as follows; On the GF plus SC media, Antifungal action augmented Significantly, especially this Characteristic was apparent on X. Citri and H.Oryzae and Conidia formation. CL plus SC media exhibited a Slightly additive action in the early stage of incubation but in Combination of GF and CL Synergistic action was inapparent, and when GF combined with chloramphenicol its action was slightly elavated.
These experiments were conducted to investigated the conditions of the phospholipid and fatty acid composition of Micrococcus halobius as a funtion of growth temperature (5 to 35℃) and NaCl content (0 to 2.0M) of the growth medium. When the growth temperature was decreased, the relative amount of mono-unsaturated branched-chain fatty acids increased. When Micrococcus halobius strain A was grown in media containing high NaCl concentrations, the relative amount of the major fatty acid, Ca 15 : 0, increased. The relative amount of anionic phospholipid also increased when the NaCl concentration of the growth medium was increased. The increase in anionic phospholipid content resulted from a decrease in the relative mole percent content of Phosphatidylethanolamine and an increase in the relative mole percent content of cardiolipin.
Present work was conducted to investigate the effects of gamma-radiation and heat. Irradiated at doses of 10krad using indoor gamma room of approximately at 10,000ci of ^(60)Co and heat 10 minutes at 47℃, 52℃, their sterilizing effect was revealed differently depending on of treatments. Irradiation heating showed a synergistic effect wheareas Pre-irradiation heating revealed the opposite effect and the effect differed slightly with heating temperature.
A Study on environmntal investigation on the well in Andong Dam for the finding the degree of Microbial distribution and Heavy metals, Inorganic ions. For this study was carried out from July, 1986 to June, 1987. The following results were obtained: The concentrations of F-, Cl-, NO_(3)- and SO_(4)^(-2) were measured 1.485, 2.375, 4.993, 12.88 (Total averge ppm) and heavy metals of Cd, Cu, Hg, Mn, Pb, Zn were ND, 2.3t-0.3~ND, ND, 0198~ND, 0135~ ND. 1555 (Total average ppm). In the microbial distribution, site A-l, A2 basin showed Enterobacter hafniae, A-3~A-6, and B-l~B-4, Aeromonas hydrophilia, B-5, E. hafniae, B-6, A.caviae and C-l~C-2, Serratia liquefaciens, C-3~C-4, A. hydrophilia, C-5~C-6, E.hainiae Antibiotic sensitivity and assay tests were resistance of Ampicillin, Carbenicillin and Cephalothin, Most of other antibiotics susceptible.
Gonadal dysgenesis (GD) sterility associated with P-M system in Drosophila melanogaster from Cheju, Sokch'o and Pusan were examined in order to determine the distribution of the chromosomal P factors and the extrachromosomal cytotypes. And type conversion among the three types, P, Q and M, of the P-M system were investigated. True P elements for potential GD sterility were practically null or nearly absent in these populations. The few exceptional males showed very weak P activity of 10-20% F1 female sterility on crossing with Canton-S females. Most of the isofemale lines from the three populations showing intermediate and different levels of GD sterility. This suggests the existence of P factors and cytotype polymorphism in these populations. 61-69% of the isofemale lines from Cheju and Sokch'o populations were typical Q strain, and most of the remaining lines were M' type. But M' type is predominant in Pusan population. Among 16 isofemale lines determined the P, Q, M' and M types at the time of the first generation, about 69% (11/16)of the lines have shown type conversions during the laboratory culture for 15 generations, and 5 lines (31%) have retained their original types. These type change were P→Q, Q→M', M-M' and M'→Q. Changes from the other types to the P type were never found.
유류(油類)를 탄소원으로 하는 토양 미생물의 순수 분리 및 분해 활성에 따른 결과는 다음과 같다. 1. 토양 미생물 중 원유 성분 중에 PAHs 물질을 탄소원으로 이용하는 균주를 혼합 균주로 분리하여 유일 탄소원으로 crude oil 29%가 포함된 평판배와 액체배지에서 단일 균주로 성장을 확인하여 분리된 35번 균주를 동정한 결과 Acinetobacter lwoffii 근연종로 동정되었다. 2. 35번 균주의 원유 분해능은 GC 측정결과를 보면 배양시간이 지날수록 원유 성분이 분해되어 peak가 감소되었으며, crude oil내의 화합물의 분해양상을 보면 탄소수가 많은 물질은 시간이 지남에 따라 서서히 감소하는 경향을 보였다. 탄소수가 적은 화합물질은 배양시간이 지남에 따라 감소하다 증가하는 경우도 생기며, 다시 감소하는 경향을 보이는데, 이는 탄소수가 많은 물질이 분해가 되어 탄소구가 적은 화합물질이나 전 물질의 중간 유도체 물질로 축적되었을 경우로 추정된다. 3. 원유성분 중 탄소수가 적은 화합물질 (저분자 PAHs)을 미생물이 쉽게 분해한다. 따라서 탄소구가 많은 화합물질 (고분자 PAHs)을 쉽게 분해하는 미생물을 복합적으로 이용하여 탄소수가 적은 물질로 만든다면 PAHs 물질을 분해하는 효과가 더욱 클 것으로 사료된다. 4. 본 연구를 통해 토양 시료로부터 분리된 미생물이 탄소원으로 이용되는 물질 중, PAHs 물질을 이용하여 증식할 수 있다는 것을 알게 되었다. 이와 같이 유류계 유사물질을 탄소원으로 이용하는 Acinetobacter sp.와 유사한 미생물을 자연계로부터 탐색하여 응용함으로써 오염된 환경의 정화 및 환경오염 방제를 유도할 수 있을 것으로 사료되며, 환경학적으로 안정된 생물학적 처리 기술 개발을 위한 기초연구로서 활용 가치가 높다. This study was carried out to isolate soil microorganisms, which can emulsify crude oil by using liquid culture including crude oil (2% v/v). The strain 35 of them was screened to have the most significant biodegradable activity and oil conversion rate. Strain 35 was identified morphological, biological and physiological characteristics with 35, as Acinetobacter lwoffii. The strain 35 showed the crude oil emulsified activity, crude oil degradable activity, respectively. As a result of cell growth and emulsifying activity as a function of time were also determined. Crude oil degradation and the reduction of product peaks were identified by the analysis of resident oil by gas chromatograph and mass spectrometry. Thus it is belived that Acinetobacter lwoffii 35 use a variety of hydrogen carbonate as carbon resource.
영남권 일대의 용수로서 중요한 위치를 차지하고 있는 안동댐의 가두리 양식장 수역의 미생물 오염과 이들의 생화학적, 생리적 특성은 1994년 4월, 6월, 8월 3회에 걸쳐 13개 정점에서 조사, 연구하였다. 우점종을 나타낸 13개 균속, 18개 종은 Enterobacteriaceae과의 Citrobacter diversus, C. freundii, Klebsiella axytoca, K.terrigena, Enterobacter. Cloacae, E. agglomerance, Erwinia herbicola, Serratia plymuthica, Hafnia alvei. Vibrionaceae과의 Aeromonas hydropyila. Micrococcaceae과의 Staphylococcus xylosus. 타속의 Bacillus subtilis, B.megaterium. Pseudomonadaceae과의 Pseudomonas aeruginosa, Pseudo. Putida. Neisseriaceae과의 Acinetobacter caleoaceticus. 타속의 Flavobacterium breve, Alcaligenes faecalis로 나타났으며, 안동댐 수질의 미생물 오염중 대장균과 분원성 대장균 분포가 높았다. The bacterial contamination and biochemical, physiological characteristics were studied in Andong Dam by artificial fishes cultivate, during April, June, August, 1994. The bacterial dominant species were 18 species of 13 genus. The results was obtain as follows: The species of the genus Citrobacter were C. diversus and C. freundii, klebsiella were K. oxytoca and K. terrigenu, Enterobacter were E. agglomerance, Erwiniaviere E. herbicola, Serratia were S. plymuthica, Hafnia were H. alvei of the Enterobacteriaceae. Aeromonas were A. hydrophila of the Vibrionaceae. Micrococcus were Staphylococcus xylosus of the Micrococcaceae. other genus were Bacillus subtilis and B. megaterium. Pseudomonas were P. aeruginosa and P.Pulida of the Pseuiomonadaceae. Other genus were Flavobacterium breve and Alcaligenes faecalis. The bacterial contamination of the water quality in Andong Dam showed higher coliform and fecal coliform.
Scabby barley collected in kyung Buk, Yung Poong Gun district fed to healthy 2 year cows, and detcermination of Choline-Esterase Activity in Blood of cows. The results obtained in this investigation are Summarized as follows. 1. Choline-Esterase Activity in the blood of cows fed scabby barley has beenn decreased. 2 The poisonous component of the Scabby barley thought to be Anticholinesterase and hyocy-amine, atropin, Scopolamine.