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      • KCI등재후보

        Growth and Amino Acid Contents of Spirulina platensis with Different Nitrogen Sources

        오희목,김성건,윤병대,Aeran Choi 한국생물공학회 2003 Biotechnology and Bioprocess Engineering Vol.8 No.6

        The growth and amino acid contents of the cyanobacterium, Spirulina platensis strain NIES 46, were investigated using ammonium, nitrate, nitrite, or urea as the sole nitrogen source in a batch culture. Chlorophyll a concentration was highest at 2,096 mg/L in the nitrate group after 10 days of cultivation, while the dry weight of S. platensis was highest at 4.5 g/L in the ammonium group after 30 days of cultivation. The total amino acid content was highest at 174 mg/g dry weight of S. platensis in the urea group at the end of the cultivation period, yet the amino acid patterns for S. platensis were similar for all the experimental groups. Therefore, it seemed that the growth and amino acid composition of S. platensis varied depending on the type of nitrogen sources, while the amino acid patterns were not changed. Also, the most efficient harvesting time for S. platensis seemed to be approximately 10 days after cultivation.

      • KCI등재

        Kinetic correlation between degradation and dechlorination of perchloroethylene in the Fenton reaction

        오희목,Hee-Sik Kim,Wan-Suk Lee,Chi-Yong Ahn,Byung-Hyuk Kim,Jang-Eok Kim 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.6

        In the Fenton reaction, degradation and dechlorination are directly affected by the concentrations of hydrogen peroxide and Fe3+. Although there is considerable research on the biodegradation of chlorinated compounds combined with the Fenton reaction, the kinetics of degradation and dechlorination of the reaction, with various concentrations of hydrogen peroxide and Fe3+, have been rarely investigated. Therefore, we investigated the degradation and dechlorination of PCE with various concentrations of hydrogen peroxide and Fe3+. The initial concentration of PCE (10 μM) decreased from a value of 8.9 μM (with 0.1 mM of hydrogen peroxide and 5 mM of Fe3+) to 1.1 μM (with 10 mM of hydrogen peroxide and 5 mM of Fe3+); the respective values for chloride ions produced were 0.9 and 21.6μM. Also, the initial 10 μM of PCE decreased from 8.9 (with 0.1 mM of Fe3+ and 5 mM of hydrogen peroxide) to 2.2μM (with 10mM of Fe3+ and 5 mM of hydrogen peroxide); the respective chloride ions produced were 0.7 and 14.5μM. The logarithmic correlations between the degradation and dechlorination coefficients were 0.7682 and 0.7834for concentrations of hydrogen peroxide and Fe3+, respectively. Both coefficients were used, from all possible cases,to derive six models which displayed both the ratio of degradation and dechlorination and the hydrogen peroxide and Fe3+ concentrations. The dechlorination of PCE could then be predicted with the model obtained by the coefficient with the concentration of hydrogen peroxide and Fe3+. The models could be applied to various Fenton reactions for optimization of degradation or dechlorination, such as biodegradation of PCE which is scarcely degraded by aerobic bacteria.

      • SCOPUSKCI등재

        반연속배양의 혼합균주에 의한 Benzene, Toluene 및 Phenol 혼합물 분해

        오희목,김서인,이창호,서현효,이문호,고영희,윤병대 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.4

        Benzene, toluene 및 phenol을 각기 250 ㎎/ℓ 포함한 합성폐수에 혼합균주인 GE1을 접종하고 반연속배양에 의하여 군집의 안정을 이룬 후 방향족화합물의 생분해에 대하여 조사하였다. 미생물 무처리구인 대조구에서 75 ㎖/ℓ/min의 폭기에 의해 배양 24시간에 phenol은 37%, benzene은 83%가 휘발되고, toluene은 배양 12시간에 모두 휘발되었다. Benzene과 toluene의 생분해는 섬모상 고정화 담체를 설치한 SB(strains+biofilm) 처리구에서 효과적이며, phenol은 glucose를 첨가한 SG(strains+glucose) 처리구에서 보다 신속히 분해되었다. Benzene, toluene 및 phenol을 250 ㎎/ℓ 또는 1000 ㎎/ℓ 첨가하였을 때 SG 처리구는 16시간이나 32시간에 이들 화합물을 완전히 제거하였다. COD 제거율은 SG 처리구에서 초기에 80 ㎎/ℓ/h로 가장 높았으나, 증식된 균체량에 의하여 배양 12시간 이후에는 116∼140 ㎎/ℓ의 일정한 값을 보였다. 결론적으로 혼합균주 GE1은 benzene, toluene 그리고 phenol 등의 방향족화합물이 혼합된 산업폐수의 처리에 폭 넓게 사용될 수 있는 가능성을 보였다. The biodegradation of aromatic compounds by a mixed culture GE1 was investigated in an artificial wastewater containing 250 ㎎/ℓ of benzene, toluene, and phenol in semicontinuous culture. In the control group (no strains) with an aeration rate of 75 ㎖/ℓ/min, 37% of phenol and 83% of benzene were volatilized during early 24 hrs and toluene was disappeared form the medium within 12 hrs. The biodegradation of benzene and toluene was effective in SB (strains+biofilm) treatment, while phenol was degrade more quickly in SG (strains+glucose) treatment including glucose as an additional carbon source. Aromatic compounds added at a concentration of 250 ㎎/ℓ or 1000 ㎎/ℓ were completely removed by SG treatment after 16 hrs or 32 hrs, respectively. The removal rate of COD was high as much as 80 ㎎/ℓ/h in SG treatment during early period, but COD revealed a stable value of 116∼140 ㎎/ℓ after 12 hrs caused by increased biomass. Therefore, it is concluded that the mixed culture GE1 could be used for the wastewater treatment including aromatic compounds such as benzene, toluene, and phenol.

      • SCOPUSKCI등재

        Naphthalene 분해균주 Alcaligenes sp. A111 분리 및 특성

        오희목,강정현,이창호,박찬선,안성구,윤병대,고영희 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.4

        Naphthalene을 증기상으로 공급하고 배양하면서 세균집락 형성이 우수하고, 액체배양에 의한 균체증식이 탁월한 균주를 선별하여 Alcaligenes sp. A111로 동정하였다. Alcaligenes sp. A111의 생육을 위한 최적 온도는 30℃, 최적 pH는 7.0이며, 배양 후 12시간부터 급속한 균생장을 보여 배양 48시간에는 거의 정지기에 도달하였다. Naphthalene 농도에 따른 상대생장율은 영양염류의 농도에 대한 전형적인 미생물생장의 유형을 따르는 것으로서, 최대 상대생장율은 2.8/day이며 500∼4000 ppm의 농도에서 더 이상의 증가가 없었다. 무기질소원으로는 NH_4Cl과 NH_4NO_3에서 그리고 인과 질소의 질량비는 약 6 : 1에서 균생장이 우수하였다. Alcaligenes sp. A111는 catechol, gentisic acid, salicylic acid 등을 효과적으로 이용하며, benzene, toluene 및 octane에 대하여 내성을 보임으로서 이들 물질이 naphthalene과 혼합된 산업폐수의 처리에 효과적으로 이용될 수 있는 균주로 판단되었다. A bacterial strain which formed a distinct colony on agar plate containing naphthalene as a vapor phase and grew well in a liquid minimal medium was isolated and identified as Alcaligenes sp. A111. Optimum temperature and pH for the cultivation of Alcaligenes sp. A111 were 30℃ and 7.0, respectively. Cell growth increased dramatically from 12 hours after inoculation and revealed a stationary phase at about 48 hours. Relative growth rate (μ’) increased hyperbolically depending on the concentration of naphthalene up to 500 pm and reached to the maximum value of 2.8/day, but μ’ didn’t change within a range of 500∼4000 ppm naphthalene. NH_4Cl or NH_4NO_3 was preferred as a nitrogen source and a P : N ratio by weight of 6 : 1 was favorable to cell growth. Alcaligenes sp. A111 utilized the intermediates of degradation of naphthalene and showed tolerance to benzene, toluene, and octane. Therefore, it is suggested that Alcaligenes sp. A111 could be effectively used for the biological treatment of wastewater containing naphthalene in the presence of some aromatic compounds.

      • 부영양 수질에서 남조류의 일주성 수직이동

        오희목,맹주선 인제대학교 낙동강환경연구소 1995 심포지움(인제대학교 부설 환경연구소 발표논문집) Vol.- No.4

        부영양 수질에서 남조류의 우점과 수직이동의 원인을 규명하고져, 대청호에서 수화현상이 나타나는 시기에 수심에 따른 이화학적 수질, 생체량, 그리고 우점 조류종등을 분석하였으며, 실험실에서 Anabaena flos-aquae의 연속배양에 의한 인의 축적에 대하여 조사하였다. 용존 무기인과 총인은 표층과 심층간의 농도구배를 보였으며, 우점 조류종은 Microcystis sp.로 여름에 전체 식물플랑크톤 개체수의 95%이상을 차지하였다. Microcystis sp.는 10 - 14시에 약 40%가 수심 0.1 m의 표층에 분포하고 14시 이후에는 점차 심층으로 하강하여 6시에는 수심 4 m에 약 60%로 가장 많이 분포하며, 다시 표층으로 상승하는 수직이동의 양상을 보였다. 인-충분조건의 turbidostat에서 질소고정하는 A. flos-aquae는 여분의 인을 sugar-P로 저장하였다. 광포화에서 광제한 상태로 전환되면서 Q_p는 일시적으로 18 fmol/cell로 크게 증가하였다. 이것은 성충화된 부영양 수체에서 남조류가 수직하강에 의하여 심층에 풍부한 인을 흡수하여 축적할수 있음을 시사하는 것이다.

      • SCOPUSKCI등재

        Biodegradation of Benzne,Toluene, and Phenol by a Mixed Culture in Semicontinuous Culture

        오희목,김성빈,이창호,서현효,이문호,고영희,윤병대,Oh, Hee-Mock,Kim, Seong-Bin,Lee, Chang-Ho,Suh, Hyun-Hyo,Lee, Moon-Ho,Kho, Yung-Hee,Yoon, Byung-Dae 한국미생물 · 생명공학회 1994 한국미생물·생명공학회지 Vol.22 No.4

        The biodegradation of aromatic compounds by a mixed culture GE1 was investigated in an artificial wastewater containing 250 mg/l of benzene, toluene, and phenol in semicontinuous culture. In the control group (no strains) with an aeration rate of 75 ml/l/min, 37% of phenol and 83% of benzene were volatilized during early 24 hrs and toluene was disappeared from the medium within 12 hrs. The biodegradation of benzene and toluene was effective in SB (strains + biofilm) treatment, while phenol was degraded more quickly in SG (strains + glucose) treatment including glucose as an additional carbon source. aromatic compounds added at a concentration of 250 mg/l were completely removed by SG treatment after 16 hrs or 32 hrs, respectively. The removal rate of COD was high as much as 80 mg/l/h in SG treatment during early period, but COD revealed a stable value of 116~140 mg/l after 12 hrs caused by increased biomass. Therefore, it is concluded that the mixed GE1 could be used for the wastewater treatment including aromatic compounds such as benzene, toluene, and phenol.

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