임상연구병동 자동병상배정 전문가시스템 개발

송승미,김종명,김종률,신재국,김은영,Song, Seung-Mi,Kim, Jong-Myoung,Ghim, Jong-Lyul,Shin, Jae-Gook,Kim, Eun-Young 대한임상약리학회 2012 臨床藥理學會誌 Vol.20 No.1

Background: Demands for complicated and long-term administration clinical trials have been increased since investigators actively involved in early stage clinical trials including first-in-human (FIH) trials. Research wards in our clinical trial center were mainly used for phase 1 trials. In order to perform several clinical trials simultaneously during a short period with a minimum number of rooms, beds, and equipment, staffs have to spend a lot of time for efficient operation of limited numbers of facilities. In this study, automated bed-allocation system was developed for efficient scheduling of the research ward based on clinical trial condition and status like experts. Methods: The system was developed based on clinical trial design, schedule, and the information on research bed and availability stored and updated in database (DB). Automatic assignment system was designed to find an optimal schedule according to the given information using expert rules and algorithms. The optimal solution can be visualized on Gantt chart using C# and Chart FX API. Results: The system was developed to demonstrate the schedule on color chart. It turned out to be well-designed to find an optimal schedule for bed allocation. The system also allows automatic updating of the schedule and information in the DB. Conclusion: Automated bed-allocation system developed in this study could save time and improve the efficiency for using space and equipment in clinical trial center. The system can be also applied to similar works or tasks in other fields.

리소좀 교통 이상을 초래하는 뮤코지방증 2형과 3형 환자의 섬유아세포를 이용한 신규 유전자 탐색 및 돌연변이에 대한 연구

송승미,장수희,백경훈,진동규,Song, Seng Mi,Chang, Soo Hee,Paik, Kyung Hoon,Jin, Dong-Kyu 대한유전성대사질환학회 2005 대한유전성대사질환학회지 Vol.5 No.1

목적: 뮤코지방증 유형 II와 III은 리소좀 효소인N-acetylglucosaminyl-1-phosphotransferase (UDP-N-acetylglucosamine, GlcNAc-phospho-transferase)의 결손에 의해 초래되며, 상염색체 열성으로 유전되는 질환이다. ${\alpha}/{\beta}/{\gamma}$ subunit로 구성되는 이 효소의 결핍으로 인해 리소좀으로 운반되는 수십 종류의 효소들에 mannose-6-phosphate(M6P)를 부착하는 과정에 장애가 생겨 분해되지 않은 물질이 축적되어 질병이 초래된다. 이 질환에 있어서 산전 진단과 유전 상담을 위해서는 상기 효소의 해당 유전자가 밝혀져 있어야 하나, 현재 이 효소의 ${\gamma}$ subunit를 암호화하는 유전자가 GNPTAG에 해당된다고 밝혀져 있을 뿐 ${\alpha}/{\beta}$ subunit을 암호화하는 GNPTA에 해당하는 유전자는 밝혀져 있지 않고 돌연변이 역시 보고된 적이 없다. 본 연구는 리소좀 효소의 인산화에 관여하는 N-acetylglucosamine-1-phospho-transferase의 결함이 있는 환자의 섬유아세포를 이용하여 리소좀 연관 신규 유전자를 찾아내고, 그 유전자를 대상으로 돌연변이를 규명하고자 하였다. 방법: 이를 위해 5명의 환자와5명의 연령, 성별이 일치하는 정상아의 섬유아세포를 계대 배양하여 이 세포를 이용하여 수행한 subtractive hybridization을 통해 신규 유전자를 탐색하고, 신규 유전자를 대상으로 돌연변이 분석을 수행하였다. 결과: 연구 결과 환자에서 발현이 증가된 유전자 73개와 발현이 감소된 유전자 50개를 밝혀냈다. 분석된 유전자 중에서 MGC4170이환자에서는 발현되지 않으나 정상인에서는 발현됨을 발견하였고, 이 유전자가 아직 밝혀지지 않은 GNPTA로 확인되어 환자를 대상으로 돌연변이 분석을 시행하였다.분석 결과 기존에 돌연변이가 보고되었던 GNPTAG에는 돌연변이가 없었으나, MGC4170에는 7개의 돌연변이가 발견되었다. 본 연구는 GlcNAc-phosphotransferase의 ${\alpha}/{\beta}$ subunit에 해당되는 MGC4170의 최초 돌연변이 보고이다. 결론: 본 연구를 통해 뮤코지방증 II형과 III형에서 발현되는 유전자 군을 파악할 수 있었으며, 동시에 MGC4170 돌연변이를 규명함으로써 이 질환의 병리 기전 연구와 산전 진단에 기여하고자 한다. Purpose: To understand genetic differences and similarities between mucolipidosis and control. Methods: Using the fibroblast of the mucolipidosis II and control, forward and reverse subtracted libraries were constructed. Among these clones, we investigated mutations in the GNPTA (MGC4170) gene, which codes for the ${\alpha}/{\beta}$ subunits of phosphotransferase, and in the GNPTAG gene, which codes for the ${\gamma}$ subunits in 5 Korean patients with mucolipidosis type II or IIIA. Result: Several differentially expressed cDNAs were cloned and their sequences were determined. Mutation analysis of the interested gene, GNPTA was performed and we identified 7 mutations in the GNPTA gene, but none in the GNPTAG gene. The mutations in type II patients included p.Q104X(c.310C>T), p.R1189X(c.3565C>T), p.S1058X(c.3173C>G), p.W894X(c.2681G>A) and p.H1158fsX15(c.3474_3475delTA), all of which are non-sense or frame shift mutations. However, a splicing site mutation, IVS13+1G>A (c.2715+1G>A) was detected along with a non-sense or a frame shift mutation (p.R1189X or p.E858fsX3(c.2574_2575delGA)) in two mucolipidosis type IIIA patients. Conclusion: This report shows that mutations in the GNPTA gene coding for the ${\alpha}{\beta}$subunits of phosphotransferase, and not mutations in the GNPTAG gene, account for most of mutations found in Korean patients with mucolipidosis type II or IIIA.

임상연구병동 자동병상배정 전문가시스템 개발

송승미,김종명,김종률,신재국,김은영 대한임상약리학회 2012 Translational and Clinical Pharmacology Vol.20 No.1

Background: Demands for complicated and long-term administration clinical trials have been increased since investigators actively involved in early stage clinical trials including first-in-human (FIH) trials. Research wards in our clinical trial center were mainly used for phase 1 trials. In order to perform several clinical trials simultaneously during a short period with a minimum number of rooms, beds, and equipment, staffs have to spend a lot of time for efficient operation of limited numbers of facilities. In this study, automated bed-allocation system was developed for efficient scheduling of the research ward based on clinical trial condition and status like experts. Methods: The system was developed based on clinical trial design, schedule, and the information on research bed and availability stored and updated in database (DB). Automatic assignment system was designed to find an optimal schedule according to the given information using expert rules and algorithms. The optimal solution can be visualized on Gantt chart using C# and Chart FX API. Results: The system was developed to demonstrate the schedule on color chart. It turned out to be well-designed to find an optimal schedule for bed allocation. The system also allows automatic updating of the schedule and information in the DB. Conclusion: Automated bed-allocation system developed in this study could save time and improve the efficiency for using space and equipment in clinical trial center. The system can be also applied to similar works or tasks in other fields.

STANAG 4586 기반 DLI(Data Link Interface)프로토콜 적용방안 연구

송승미(Song Seung Mi) 한국통신학회 2013 한국통신학회 학술대회논문집 Vol.2013 No.11

온톨로지를 이용한 표 머리와 몸체 분리 방법

송승미(Seungmi Song),권혁철(Hyukchul Kwon) 한국정보과학회 2008 한국정보과학회 학술발표논문집 Vol.35 No.2

본 논문은, 웹상의 표를 표 머리와 몸체로 분리하는 방법으로 온톨로지 사용을 제안한다. 웹 상에서 정보 전달을 위한 목적으로 사용한 표는 표 머리와 표 몸체로 나눌 수 있다. 표 머리는 표에서 나타내고자 하는 속성이며, 그에 해당하는 실제 속성값은 표 몸체가 된다. 이를 분리하고자 기구축 되어 있는 IT Device 온톨로지를 사용하여, 온톨로지에서 class로 표현된 부분이 표에서는 표 머리 부분일 가능성이 크다는 가정하여 표 머리와 표 몸체를 분리하였다. 사용한 온톨로지의 도메인이 IT Device이므로, 실험에 사용한 표도 전자제품 관련 웹 페이지에서 추출한 표로 제한하였다.

스마트워치를 활용한 헬스케어 시스템 구성 방안에 관한 연구(K-헬스케어 시스템 사례를 중심으로)

김박철(Park-Chul Kim),송승미(Sung-Mi Song) 대한전기학회 2022 대한전기학회 학술대회 논문집 Vol.2022 No.7

인슐린 의존성 당뇨병 환아와 부모들이 호소하는 저혈당 증상에 관한 임상고찰

이병을,박화영,천정미,권은경,송승미,김정심,진동규 대한소아청소년과학회 2001 Clinical and Experimental Pediatrics (CEP) Vol.44 No.4

광 가상사설망에서 우선순위기반 최소간섭 경로 멀티캐스트 라우팅 알고리즘 연구

서상보(Sang-Bo Seo),송승미(Seung-Mi Song),이종근(Jong-Geun Lee),김성운(Sung-Un Kim) 대한전자공학회 2007 대한전자공학회 학술대회 Vol.2007 No.7

OVPN(Optical Virtual Private Network) based on DWDM (Dense Wavelength Division Multiplexing) backbone framework is considered as a promising approach for the future VPN. This paper proposes a new routing algorithm, called PMIPMR(Priority-based Minium Interference Path Multicast Routing) algorithm which finds an alternate route considering node priorities when the congestion is occurred in a network. The PMIPMR algorithm tries to improve blocking probability and wavelength utilization by avoiding congested path for potential future connection requests.

효소제 및 호르몬제의 품질관리에 관한 연구(Ⅰ) : 데옥시리보뉴클레아제 제제 Deoxyribonuclease (DNase) preparations

공학수,최돈웅,김도훈,김미정,김희성,정기숙,송승미,조수열 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-

데옥시리보?트렐이제(DNase)는 분자량 약 62kDa의 핵산(DNA)분해효소로서 소염작용이 있어 기관지염, 요도염, 낭포성섬유종등의 화농성질환의 채료에 사용되고 잇다. 이 약의 정량법으로는 효소-기질반응에 의하여 기질의 점도변화를 측정하는 효소학적 정량법이 사용되고 있으나, 숙련된 실험자를 요구하며, 시험자간의 오차가 크고, 정확성 및 재현성이 떨어지는 문제점이 제기되어 왔다.따라서 본 연구에서는 모세관전기영동분석기(Capillary Electropho-resis, CE)를 이용하여 데옥시리보뉴클레아제 및 그 함유제제에 대한 재현성과 정확성이 높은 분석법을 확립하고자 하였다. 데옥시리보뉴크레아제 원료 및 그 ㅏㅁ유제제를 가지고 모세관, Run buffer, 검출기, 표준액 및 검액 전처리법, 내부표준물질을 검토하여 모세관전기영동분석기그이 분석조건을 검토하고, 검액 및 표준액을 가지고 검량선작성, 재현성 시험, 시판품의 정량 등을 실시한 결과, 데옥시리보뉴크레아제 및 그 함유제제의 CE 분석조건으로 Capillary:50μm×40㎝,fused silica;검출기:자외부흡광광도계(측정파장 200nm);Run buffer:75mM potassium phosphate buffer (pH 2.5);Run voltage:15kV를 사용하였을 때 inter, intra-day 재현성(%RSD)이 모두 1% 이하로 양호하였다. 이 방법은 조직이 간편하고 정확하게 정량할 수 잇어 데옥시리보뉴클레아지 제제? 품질 평가법으로 활요될 수 있을 것이다. Deoxyribonuclease (DNase) is the lytic enzyme used for the treatment of purulent diseases such as broncopulmontary infections, cystic fibrosis etc. For separation, identification and deterraination of deoxyribonuclease in final drug preparations, a capillary electrophoresis (CE) method has been developed. The influeuces of the buffer pH, electrolyte composition, temperature and voltage were investigated to determine the best analytical conditions. Separation of Deoxyribonuclease was achieved with a 75mM potassium phosphate buffer (pH2.5) and UV detection (200nm). Efficient separations occur in less than 15min with good repeatability giving a relative standard deviation of less than 1%. The precision of the method was evaluated from inter-and intra-day replicate injection of deoxyribonuclease standard solutions. The products from two manufacturers were analyzed and qualitative differences between two products were compared.

한국인에서 21-hydroxylase 결핍에 의해 초래되는 선천성 부신피질 과형성의 유전자형과 임상형에 관한 연구

김흥식,박성준,이진성,이동환,진동규,오필수,김덕희,유한욱,송승미,김정심,황혜진,온화영,고시환,엄미령,신재훈,한헌석,고철우 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.2

Background : Congenital adrenal hyperplasia(CAH) results from an inherited defect in enzymatic steps required to synthesize cortisol from cholesterol. 21-hydroxylase deficiency accounts for 95% cases of CAH. It appears that the frequency and the type of the responsible mutations differ according to the ethnic background and the type of mutation can predict the clinical outcomes such as salt losing type(SL), simple virilizing type(SV) and non-classic type(NC). Methods : We have analyzed CYP21 genes in 55 Korean cases(110 chromosomes) of CAH by Southern blotting, PCR-dot hybridization and PCR amplification-created restriction site method. The patients include 43 cases of SL and 12 of SV. None of the NC was found. Results : We found the mutations in 94%(103/110) of the examined chromosomes. A total of 10 types of mutations were discovered. The mutations include aberrant splicing of intron 2(i2, 35%), CYP21 gene deletion(32%) and I172N(11%) in order. When the relationship between the clinical types and genotypes were correlated, most of the SL patients have either i2(42%) or CYP21 gene deletion(41%), while SV patients have I172N(33%) or P30L(21%). The parents' mutation was investigated in 20 cases. In 4 families, one of the parents was not the obligatory heterozygote carrier i.e. did not have a mutation. The results suggest the high incidence of de novo mutation. Conclusion : We have identified the frequency of mutations of the CYP21 in Korean AGS patients. Our results shows that the clinical type of AGS can be predicted from the genotypes of CYP21. Also the high incidence of de novo mutation of CYP21 confirmed the genetic instability of major histocompatibility III region where the CYP21 is located(J Kor Soc Endocrinol 15:237-247, 2000).