Apoptosis and necroptosis‑inducing effects of arctigenin on nasal septum carcinoma RPMI‑2650 cells in 2D and 3D culture

Yoon‑Jin Lee,Kwan‑Sik Park,Byoung Joon Baek,Kyong‑Ae Lee,Sang‑Han Lee 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Backgrounds Arctigenin derived from the seeds of Arctium lappa Linnaeus is known as an anticancer drug candidate by targeting various pathways involved in anticancer therapy. Methods Using 2D monolayer and 3D spheroid culture systems in nasal septum carcinoma RPMI-2650 cells, the effects of arctigenin and dexamethasone on cell viability, ROS levels, ATP level, mitochondrial function, apoptosis and necroptosis were examined. Results The combination treatment of both compounds induced strong cytotoxicity, accompanied by increases of sub-G0/ G1 peak, annexin V-PE-positive cells, and ROS levels, loss of mitochondrial membrane potential, and decrease of cellular ATP content. These changes were observed as simultaneous induction of DNA damage, apoptosis, and necroptosis. A series of changes by arctigenin and dexamethasone were efficiently restored by decreasing ROS levels or supplementing ATP. Treatment of 3D spheroids with arctigenin and dexamethasone decreased cell viability in the spheroids, but it was slightly resistant than cells under 2D conditions. In addition, this phenomenon was accompanied by an increase in mediators for both apoptosis and necroptosis. Conclusion Results of this study suggest that the apoptosis and necroptosis-inducing effects of arctigenin are associated with ATP depletion due to oxidative mitochondrial dysfunction.

공공기관 인력총원의 적정성 분석 : 근로복지공단 사례를 중심으로 The Case of Korea Labor Welfare Corporation

이창원,전주상,윤경준 한국정책학회 2002 韓國政策學會報 Vol.11 No.1

우리나라에서 공공기관의 인력충원방식은 해당기관이 추가업무수요에 따른 일정수의 인력충원을 요구하면 정부에서 이를 일정부분 삭감하면서 승인하는 방식을 취하여 왔기 때문에 인력충원의 적정성 여부에 대하여 논란이 일어나는 경우가 빈번하다. 따라서 본 연구에서는 이러한 문제의식을 갖고 최근에 산재보험대상 확대 조치에 따라 인력증원결정이 이루어진 근로복지공단 사례를 분석함으로써 공공기관 인력충원 적정성 판단기준의 예를 살펴보고, 이를 기초로 추가인력 예측의 방법론과 효율적인 인력활용방안을 제시하고자 한다. 이를 위하여 본 연구에서는 근로복지공단의 업무성과에 영향을 미치는 요인들을 파악하고 이를 기초로 인력충원의 적정성을 분석하였다. 분석결과 다음과 같은 연구결과를 얻었다. 첫째, 면적 대비 직원 수가 각종 업무성과에 뚜렷한 정(+)의 효과를 나타내고 있다. 둘째, 외근의 정도(외근시간비율과 여비비율)는 일관되게 업무성과에 부(-)의 영향을 미치고 있다. 셋째, 기존의 업무여건에 변화가 없다고 가정할 때, 업무확대에 따른 추가증원수치는 다소 부족한 것으로 평가된다. 이러한 결과들을 기초로 할 때 근로복지공단의 효율적인 인력관리방안으로 다음과 같은 사항들이 제시될 수 있다. 첫째, 지사 및 지역본부 인력의 최소한의 범위의 보강은 불가피한 것으로 보인다. 둘째, 특히 전체 근무 시간 중 외근의 비율이 높은 지사의 경우 외근시간을 효율적으로 관리한다는 차원에서 센타(출장소) 등을 설치하여 직원들을 현장에 전진배치시키는 것을 고려할 필요가 있다. 마지막으로, 이러한 인력보강과 센타(출장소) 등의 설치는 업무처리간소화, 정보통신기술 및 전산화 등을 통한 사무혁신 등의 수단과 동시에 진행되어야 할 것이다. This study investigates the organizational outcomes associated with the Korea Labor Welfare Corporations new legislation requiring Korean public corporations to use manpower supplements. The methods of pre-estimating additional manpower and efficient alternatives in utilizing manpower are described. The results are as follows. First, the number of the personnel in contrast with an area covered had the positive effects on the various outcome variables strikingly. Second, the extent of the outdoor service had the negative effects on the outcome variables consistently. Third, the manpower supplement was estimated to be insufficient, assuming that there was no change in business conditions. Based upon these results, preferred alternatives to those provided by the Korea Labor Welfare Corporation may be presented as follows. First, the minimum supplement of the personnel at the branch offices seems to be inevitable. Second, the establishment of a local office to manage the time of outdoor service efficiently needs to be considered, especially in the case of the branch office with high extent of the outdoor service. Third, the above two alternatives have to be implemented in combination with the means of business reforms. Such reforms include simplifying business processes and introducing both effective information technologies and computerization.

초·중등 및 대학교재 중 물질특성에 대한 연계성 연구

李京玧,文正大,文星培 釜山大學校 師範大學 1998 교사교육연구 Vol.36 No.-

Material Characteristics contents in the chemistry textbooks from elementary school to college were investigated to consider the effectiveness of curricular articulation. For this purpose, the eleven subjects of chemistry contents were selected from chemistry textbooks, and the learning purpose and contents were analyzed through the classification rules. The results of analysis showed that total articulation were relatively effective. But the gaps partly appeared in case of comparing with the level of intellectural development of student. On the based of this results a new concept diagrams were proposed through the structure diagrams considered both macro and micro aspect. To develop the curricular articulation between the school units it is important that textbooks should be with the consistent plan after the discussion with the actual teachers of the school units.

백서의 우울모형에서의 칼슘통로 길항제 에타베린(Ethaverine)의 항우울효과

이상경,김선희,김록우,윤성환,김용관,김경태,김영훈 대한신경정신의학회 2000 신경정신의학 Vol.39 No.2

연구목적: 본 전임상연구에서는 우울증의 두가지 동물모형을 통해 에타베린이 단독 혹은 이미프라민과 병용처치 되었을 때의 효과를 알아보고자 하였다. 방법: 표준화된 검사법인 강제수영검사를 사용하여 옹성백서에게 에타베린(20mg/kg) 및 이미프라민 (20mg/kg)을 단독 혹은 병용 처치하였을 때의 부동시간의 변화를 관찰하였고, 경도의 만성 스트레스를 3주간 가한 후 4주간 에타베린과 이미프라민을 단독 혹은 병용 처치하였을때의 자당섭취량의 변화를 관찰하였다. 결과: 약물을 1회 단기처치 및 7일간 장기처치한 강제수영검사에서 이미프라민 및 에타베린 단독처치는 대조군에 비하여 부동시간의 단축을 보였고 에타베린은 이미프라민의 부동시간 감소에 대한 증강작용을보였다. 경도의 만성스트레스 실험에서도 3주간의 스트레스로 인하여 감소된 백서의 자당섭취량을 이미 프라민 및 에타베린이 회복시켰으며, 치료초기에 두 약물의 병용처치효과가 두드러졌다. 결론: 본 연구의 결과는 에타베린이 향우울 효과를 가지고 있음을 시사하며, 삼환계 항우울제인 이미프라민의 항우울효과를 증강시키므로 에타베린의 임상적 활용 가능성을 보여준다. Objectives: This pre-clinical study was performed to assess the effects of ethaverine in the two kinds of behavioral models of depression in rats. Methods: We observed the changes of the immobility time in the froced swimming test and the quantity of sucrose consumed in the chronic mild stress model, using ethaverine(20mg/kg) alone, imipramine(20mg/kg) alone, or ethaverine and imipramine concomitantly. Results: In the forced swimming test, both single treatment and chronic treatment(for 7days) with imipramine or ethaverine significantly reduced the immobility time, and concomitant chronic treatment with ethaverine potentiated the effect of imipramine. In the chronic mild stress model, both imipramine and ethaverine reversed the decreased sucrose consumption induced by 3-week stress and concomitantly treated ethaverine potentiated the effect of imi-pramine in the early phase of treatment. Conclusions: The data suggest that ethaverine can be used alone or concomitantly with other antidepressants in the clinical situation.

웹마이닝을 이용한 M-Commerce 추천시스템 설계 및 구현

이경호,윤창현,박두순 한국컴퓨터교육학회 2003 컴퓨터교육학회 논문지 Vol.6 No.3

추천 시스템들은 고객들이 E-Commerce 사이트에서 상품들을 사는 것을 도와주기 위해서 지속적인 증가추세로 사용되었다. 추천시스템들은 다양한 고객들의 선호도에 따라 유사성과 비유사성에 대한 정보의 기초위에서 고객들의 잠재적인 관심 항목들에 대해 개인의 취향에 맞게 추천하는 기술들은 제공한다. 그러나 추천시스템에 많은 관심을 가짐에도 불구하고 그들의 성능에 대한 공개된 기술이나 정보는 매우 제한적이다. 본 논문에서는, 과거 고객들의 구매행동, 고객정보, 데이터마이닝의 연관규칙을 이용한 M-Commerce 추천시스템을 설계하고 구현하였다. Recommender systems are being used by an ever-increasing number of E-Commerce sites to help consumers find products to purchase. Recommender Systems offer a technology that allows personalized recommendation, of items of potential interest to users based on information about similarities and dissimilarities among different user’ tastes. However, despite enormous interest in recommender systems, both the number of available published techniques and information about their performance arc limited. In this paper, we design and implement an M-Commerce recommendation systems using the past buying behavior of the consumer, consumer information, and association rule mining.

프락토올리고당이 스폰지 케�揚�물리적, 관능적 특성에 미치는 영향

이경애,이윤진 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

Effects of replacement of sucrose with fructooligosaccharide(FO) on the sponge cake quality were investigated. As FO level increased, the specific gravity of batter decreased. Adding FO increased the specific volume and expansion ratio. Especially, the 40% FO replacement cake showed the highest specific volume and expansion ratio. A higher degree of FO replacement for sucrose made cakes darker and more moist. The 40% FO replacement cake showed good acceptability.

특1급 호텔과 특2급 호텔 간 호텔정보시스템 품질 비교

이병원,윤병섭,이경구 대한경영학회 2003 大韓經營學會誌 Vol.16 No.7

The purpose of this study is to analyse the critical differences of the hotel information system's EDP(electronic data processing) internal control and quality between 1st 5star hotels and 2nd 5star hotels. This paper examines the relationships of the hotel information system's internal control and quality with that of user satisfactions. This study sets out the research model of influencing factors and the performance of the hotel information system through theoretical studies based on Management Information Systems, and then empirically tests hypotheses related to the model. The model was tested by multiple regression analysis using SPSS 10.0 on a sample of 618 respondents who have worked with the hotel information system in 5star hotels. The major results of this study are as follows: First, differences between 1st 5star hotels and 2nd 5star hotels are statistically significant influences on EDP internal control. Second, information quality, system quality and service quality of 1st 5star hotels are more statistically significant influences on the hotel information system's quality than that of 2nd 5star hotels. Third, EDP internal control has a statistically significant influence on the hotel information system's quality. The study's findings also indicate that EDP internal control of hotel information system affects differently on the quality of hotel information system according to grade of hotels.

ECAP 공정을 거친 1050 Al 합금 판재의 전기화학 특성에 관한 연구

정민경,최윤석,김정구,김영만,이재철,석현광 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.8

The effect of equal channel angular pressing (ECAP) pass number on the electrochemical properties of AA 1050 (UNS A91050) in 0.1M Na₂SO₄ solution containing 100 ppm chloride were investigated by electrochemical techniques (potentiodynamic test, potentiostatic test, electrochemical impedance spectroscopy (EIS) and surface analyses (OM, SEM, EDS). The ECAPed specimens that have a various numbers of passes from N=l to N=5 were selected for electrochemical experiments in this study. The results of potentiodynamic test indicated that the breakdown potential increased with increasing the ECAP pass number. EIS measurements also showed that the corrosion resistance of the AA 1050 increased with increasing the ECAP pass number at the passivation and breakdown states. The results of surface analyses (SEM, EDS) showed that silicon-containing impurities were observed on the surface of the alloy. It was found that the size of these Si-containing impurities decreased with increasing the ECAP pass number. The Si-containing impurities induced the microgalvanic reaction(i) between the Al matrix and the Si-containing mixed oxide and (ii) between the Al matrix and the Si-containing impurities. Due to its ultrafine-grained microstructure, ECAPed AA 1050 had smaller Si-containing impurities, and reduced microgalvanic currents. Thus, in the case of ECAPed AA 1050, it has increasing corrosion resistance with increasing ECAP pass number because of its small cathodic impurities.

폐경기 여성에서의 Cyclofenil의 효능에 관한 연구 : 갱년기 장애 및 Ca 장흡수에 관하여

노흥규,윤상임,성기양,이경숙,이강욱 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

In postmenopausal syndrome, most common symptoms are those of vasomotor irritability such as hot flush and palpitation, irritable bladder symptoms and dyspareunia according to atrophy of urogenital epithelium, anxiety, nervousness, depression and insomnia. Approximately 40 percent of women in postmenopausal period develop symptoms serious enough to seek medical assistance. Among non-steroid agents, cyclofenil(4, 4' cyclohexidene methlyene diphenoacetate) is known to be a quite interesting substance according to the action at the level of the hypophyseal and hypothalamic centers by reducing the incretion of FSH and restoring normal endorphin, dopamine and norepinephrine activities, thus favorably affecting the whole postmenpausal neurovegutative symptomatology. In order to evaluate effect of cyclofenil on postmenopausal symptomatology and calcium metabolism, we examined 32 patients with postmenopausal syndrome before and after 200mg/day of oral cyclofenil treatment for 30-60 days. The results were as follows: 1. With 30 days of cyclofenil administration, subjective symptoms such as facial flush, palpitation, anxiety, nervousness and dyspareunia were improved significantly (p<0.001, p<0.001, p<0.01, p<0.005, respectively). But insomnia and depression were improved significantly after 60 days of cyclofenil administration (p<0.05, p<0.001, respectively). 2. Intestinal calcium absorption rate disclosed tendency of improvement without statistical significance 30 days after cyclofenil treatment. 3. Serum calcium and phosphorus level were not changed significantly before and after cyclofenil. 4. There was no definite evidence of side effects such as uterine bleeding due to cyclofenil. In conclusion, we thought that cycofenil is effective in management of postmenopausal syndrome but for the effect on calcium metabolism, further evaluation should be needed.

오염된 완충용액으로 인한 Alcaligenes xylosoxidans의 가유행성 발생

김옥선,윤성원,백경란,박선영,이남용,송재훈 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.1