Cariporide Enhances the DNA Damage and Apoptosis in Acid-tolerable Malignant Mesothelioma H-2452 Cells

이윤진,배진호,김수아,김성호,우기민,남해선,조문균,이상한 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.8

The Na+/H+ exchanger is responsible for maintaining the acidic tumor microenvironment through its promotion of the reabsorption of extracellular Na+ and the extrusion of intracellular H+. The resultant increase in the extracellular acidity contributes to the chemoresistance of malignant tumors. In this study, the chemosensitizing effects of cariporide, a potent Na+/H+-exchange inhibitor, were evaluated in human malignant mesothelioma H-2452 cells preadapted with lactic acid. A higher basal level of phosphorylated (p)-AKT protein was found in the acid-tolerable H-2452AcT cells compared with their parental acid-sensitive H-2452 cells. When introduced in H-2452AcT cells with a concentration that shows only a slight toxicity in H-2452 cells, cariporide exhibited growth-suppressive and apoptosis-promoting activities, as demonstrated by an increase in the cells with pyknotic and fragmented nuclei, annexin V-PE(+) staining, a sub-G0/G1 peak, and a G2/M phase-transition delay in the cell cycle. Preceding these changes, a cariporide-induced p-AKT down-regulation, a p53 up-regulation, an ROS accumulation, and the depolarization of the mitochondrial-membrane potential were observed. A pretreatment with the phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002 markedly augmented the DNA damage caused by the cariporide, as indicated by a much greater extent of comet tails and a tail moment with in-creased levels of the p-histone H2A.X, p-ATMSer1981, p-ATRSer428, p-CHK1Ser345, and p-CHK2Thr68, as well as a series of pro-apoptotic events. The data suggest that an inhibition of the PI3K/AKT signaling is necessary to enhance the cytotoxicity toward the acid-tolerable H-2452AcT cells, and it underlines the significance of proton-pump targeting as a potential therapeutic strategy to overcome the acidic-microenvironment-associated chemotherapeutic resistance.

독일 보육 정책, 변화와 시사점

이윤진 육아정책연구소 2019 육아정책포럼 Vol.60 No.-

독일은 동·서독 통일의 과정 이후 현재까지 돌봄 정책의 형식과 내용에 있어 많은 변화를 거듭하여 오고 있다. 특히 보육정책으로 대표되는 돌봄 정책의 경우 가정 내 돌봄이 주로 이루어졌던 서독과 공보육시설이 발달했던 동독이 많은 차이를 내포하고 있었다고 보인다. 최근 독일은 저출산을 극복하고 있는 모습을 보이고 있는데 이는 관련 돌봄 정책의 변화와도 무관치 않아 보인다. 일부 주를 시작으로 전국적으로 확대될 조짐을 보이는 무상보육의 시행 및 보육시설의 운영과 관련한 수많은 논의들은 이를 뒷받침한다. 통일 한국이라는 과업 달성이 필연적 운명인 한국에게 이러한 독일의 돌봄 정책 변화는 서비스 지원과 비용지원, 시간지원으로 이루어지는 돌봄 정책의 정합성을 고려하여 향후 관련 정책 수립에 있어 다양한 정책적 시사점을 제공한다.

관계대명사 That과 보문자 That에 관한 논쟁

이윤진,강문구 사단법인 한국융합기술연구학회 2023 아시아태평양융합연구교류논문지 Vol.9 No.1

That in a relative clause is grammatically regarded as a relative pronoun. Compared with wh-relatives, it has been acknowledged that the relative clause does not have all the roles of relative pronouns through previous research. Since relative pronouns act as conjunctions and pronouns, they play a syntactic role as a noun, but they do not apply in some parts. So, for a long time, transformational grammarians have been insisting that that is not a relative pronoun. In this paper, from a neutral standpoint, in is revealed that the relative clause that has the characteristics of a pronoun, but cannot be considered a complete pronoun. 관계절에서 사용되는 that을 문법적으로 관계대명사라고 보통 지칭한다. 하지만 wh-관계사와 비교하면 관계절 that은 관계대명사의 역할을 모두 갖추고 있다고 볼 수가 없다는 사실을 선험 논문들을 통해 인정하게 된다. 관계대명사란 접속사와 대명사 역할을 하기 때문에 명사적인 통사적 역할을 하는데 몇 가지 부분에서 해당되지가 않는다. 그래서 오래전부터 변형 문법가들은 that을 관계대명사가 아니라고 계속 주장해오고 있다. 본 논문에서는 중립적인 입장에서 관계절 that은 대명사의 특징을 갖고는 있으나 완전하게 대명사라고 볼 수는 없다고 밝혔으며 현재 관계대명사 that은 고대영어 때 접속사에서 시작하여 대명사로 점점 확립되고 있는 과정 중이기에 아직 끝나지 않는 상태로 볼 수 있겠다.

이산화염소에 의한 수돗물의 살균효과

이윤진,최종헌,우달식,남상호 대한환경위생공학회 1998 대한환경위생공학회지 Vol.13 No.3

Temporal lobe epilepsy surgery in children versus adults: from etiologies to outcomes

이윤진,이준수 대한소아청소년과학회 2013 Clinical and Experimental Pediatrics (CEP) Vol.56 No.7

Temporal lobe epilepsy (TLE) is the most common type of medically intractable epilepsy in adults and children, and mesial temporal sclerosis is the most common underlying cause of TLE. Unlike in the case of adults, TLE in infants and young children often has etiologies other than mesial temporal sclerosis,such as tumors, cortical dysplasia, trauma, and vascular malformations. Differences in seizure semiology have also been reported. Motor manifestations are prominent in infants and young children,but they become less obvious with increasing age. Further, automatisms tend to become increasingly complex with age. However, in childhood and especially in adolescence, the clinical manifestations are similar to those of the adult population. Selective amygdalohippocampectomy can lead to excellent postoperative seizure outcome in adults, but favorable results have been seen in children as well. Anterior temporal lobectomy may prove to be a more successful surgery than amygdalohippocampectomy in children with intractable TLE. The presence of a focal brain lesion on magnetic resonance imaging is one of the most reliable independent predictors of a good postoperative seizure outcome. Seizure-free status is the most important predictor of improved psychosocial outcome with advanced quality of life and a lower proportion of disability among adults and children. Since the brain is more plastic during infancy and early childhood, recovery is promoted. In contrast, long epilepsy duration is an important risk factor for surgically refractory seizures. Therefore, patients with medically intractable TLE should undergo surgery as early as possible.

Moyamoya syndrome occurred in a girl with an inactive systemic lupus erythematosus

이윤진,연규민,남상욱,김수영 대한소아청소년과학회 2013 Clinical and Experimental Pediatrics (CEP) Vol.56 No.12

We report the case of a 17-year-old Korean girl with systemic lupus erythematosus (SLE) who presented with sudden weakness of the right-sided extremities and dysarthria. Oral prednisolone was being taken to control SLE. Results of clinical and laboratory examinations did not show any evidence of antiphospholipid syndrome or thromboembolic disease nor SLE activity. Cerebral angiography showed stenosis of the left internal carotid artery and right anterior cerebral artery with accompanying collateral circulation (moyamoya vessels). After the patient underwent bypass surgery on the left side, she recovered from the neurological problems and did not experience any additional ischemic attack during the 14-month follow-up period. This case represents an unusual association between moyamoya syndrome and inactive SLE (inactive for a relatively long interval of 2years) in a young girl.

Acute disseminated encephalomyelitis in children: differential diagnosis from multiple sclerosis on the basis of clinical course

이윤진 대한소아청소년과학회 2011 Clinical and Experimental Pediatrics (CEP) Vol.54 No.6

Acute disseminated encephalomyelitis (ADEM) is a demyelinating disease of the central nervous system (CNS) that typically presents as a monophasic disorder associated with multifocal neurologic symptoms and encephalopathy. ADEM is considered an autoimmune disorder that is triggered by an environmental stimulus in genetically susceptible individuals. The diagnosis of ADEM is based on clinical and radiological features. Most children with ADEM initially present with fever, meningeal signs, and acute encephalopathy. The level of consciousness ranges from lethargy to frank coma. Deep and subcortical white-matter lesions and gray-matter lesions such as thalami and basal ganglia on magnetic resonance imaging (MRI)are associated with ADEM. In a child who presents with signs of encephalitis, bacterial and viral meningitis or encephalitis must be ruled out. Sequential MRI is required to confirm the diagnosis of ADEM, as relapses with the appearance of new lesions on MRI may suggest either multiphasic ADEM or multiple sclerosis (MS). Pediatric MS, defined as onset of MS before the age of 16, is being increasingly recognized. MS is characterized by recurrent episodes of demyelination in the CNS separated in space and time. The McDonald criteria for diagnosis of MS include evidence from MRI and allow the clinician to make a diagnosis of clinically definite MS on the basis of the interval preceding the development of new white matter lesions,even in the absence of new clinical findings. The most important alternative diagnosis to MS is ADEM. At the initial presentation, the 2disorders cannot be distinguished with certainty. Therefore, prolonged follow-up is needed to establish a diagnosis.

上下關係ファクタ-における敬語行動- 謙讓表現を中心に -

이윤진 한국일본어학회 2007 日本語學硏究 Vol.0 No.18

본고는 인간관계 안에서 언어사용에 관한 가장 기본적이 요소로서 상하관계를 채택하여 그 상하 관계요소를 중심으로 겸양어의 양상에 대하여 고찰하였다. 연구방법으로서는 여러 인간관계가 등장하는 드라마, 소설의 회화용례에 나타나는 화자와 청자, 화제의 인물 사이의 관계를 중심으로 사회적 관계에서 성립되는 대우행동인 본질에 입각하여, 상관계를「지위의 상하․입장의 상하․ 경험의 상하․연령의 상하․친족의 상하」로 나누어 보았다. 현대 일본어의 경어행동을 보면 다양한 언어상의 배려 위에 이루어지고 있다. 「주위의 인간관계로의 배려」가 현재 경어행동에 강하게 작용되고, 언어주체의 대상에 대한 배려, 화자의 언어주체 자신에 대해서 겸양어로 이루어지는 것으로 밝혀졌다. 현대경어가 배려 표현이라고도 불리고 있는 것은 행위자 측에 시점을 둔 언어사용이며, 언어주체 대 상에대한 배려와, 언어주체 행위자 자신에 대한 겸양표현 이라고 생각할 수 있으며 현대경어 행동은 인간관계의 배려차원을 무엇보다 우선시하여 이와 같은 경어행동에서 겸양어를 사용하는 빈도가 많아지고 있다고 생각한다.

社會的ファクタ-における敬語表現の一考察

이윤진 단국대학교 일본연구소 2007 일본학연구 Vol.21 No.-

社會的ファクターにおける敬語表現の一考察 本稿では、敬語の役割を考える際、特に社會的ファクターの中の話し手․聞き手․話題の人物との人間關係に關わる內部關係の変數として作用する諸ファクターの上下․立場(恩惠授受/强弱)․內外․親疎關係と切り放して考えることができないことに着目して、話し手と聞き手․話題の人物のそれぞれの關係を社會的ファクターの中で考察した。 話し手は聞き手に對して常に身分や上下關係を意識して待遇表現を行わなければならなかった。聞き手を認識して位置づけ身分の序列や上下關係のファクターに応じて敬語表現を使い分けていた。話し手は聞き手に對して立場關係や親疎關係を優先する傾向が强くなった。 會社の上下關係で大いに影響されるのは、職の肩書であるが、內關係では年齡の上下が何よりも優先されことから考えると、會社の同じ部署の話し手と聞き手は「身內關係」になるため、身內關係の血緣關係でもっとも優先される年齡の上下ファクターが重視されている。 外關係ファクターでは、年齡や地位の上下關係よりも、立場(恩惠授受․强弱)關係․親疎關係ファクターに優先される傾向を見せる。立場(恩惠授受․强弱)關係ファクターは以前も今後も持續されると思う。 社會的ファクターから相違はみられるが、話し手の發話が、「聞き手側待遇」と「話題者側待遇」とに二分化した場合、現代の敬語体系は「聞き手側待遇」の配慮が優先されるとみられる。

돌봄노동자의 근로자성과 사회보장: 영유아 아이돌보미를 중심으로

이윤진 한국사회보장학회 2018 한국사회보장학회 정기학술대회 Vol.2018 No.1