In vitro Mammalian Chromosomal Aberration Test of Fullerene-C60

Soo Jin Kim(김수진),Kyung Taek Rim(임경택),Hae Won Cho(조해원),Jeong Hee Han(한정희),Hyeon Yeong Kim(김현영),Jeong Sun Yang(양정선) 환경독성보건학회 2009 환경독성보건학회지 Vol.24 No.1

Fullerene의 유전독성을 평가하기 위하여 Chinese hamster유래의 난소유아세포(CHO-K1 cell)를 이용하여 직접법(-S9)과 대사활성화법(+S9 mix)의 염색체이상시험을 실시하였다. 시험물질은 1% CMC 나트륨염의 현탁액(l% CMC 용액)에 희석하여 조제하였다. 대사활성화를 시키지 않은 직접법의 염색체이상시험에서 24시간 투여군은 8단계의 농도(0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10mM)로 투여하여 실시하였다. 투여 농도 증가에 따른 염색체이상의 빈도가 증가하는 양상이 나타나지 않았다. 48시간의 투여군에서는 8단계의 농도(0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10mM)로 투여하여 실시하였는데 투여 농도 증가에 따른 염색체이상의 빈도가 증가하는 양상이 나타나지 않았다. 배수체의 염색체이상은 직접법에서 관찰되지 않았다. 대사활성화법을 이용하여 6시간 시험물질을 투여한 시험에 있어서는 8단계의 용량단계 (0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10mM)를 설정하였는데 투여 농도가 증가함에 따른 염색체이상빈도의 증가양상이 관찰되지 않았다. 이상의 결과를 종합할 때 본 시험물질은 본 시험 조건하에서 CHO-K1세포에서 대사활성화를 시켰을 때 염색체 이상을 유발하지 않는 것으로 판단된다.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

肝吸蟲症에 있어서 間接螢光抗體反應(IFAT)을 이용한 血淸 및 濾紙吸着血液의 抗體價에 관한 比較硏究

趙秉洙,嚴基善,林漢鍾 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.3

The indirect fluorescent antibody test (IFAT) for clonorchiasis was performed with sectioned adult worm antigen of Clonorchis sinensis using sera and blood collected on filter paper. Total 277 sera were tested which were obtained from 171 confirmed cases of clonorchiasis, 33 cases of parasitic infection other than Clonorclzis sinensis and 70 cases of healthy individuals from endemic or nonendemic areas of clonorchiasis. The serum IgG titer 1:16 or over was regarded as positive. Thirty one out of thirty three (93.9%) sera from persons with parasitic infections other than Clonorchis sinensis were negative. Serum IgG titers of 70 healthy controls showed 1:8 or below. IFAT of clonorchiasis sera with sectioned antigen of adult worm showed specific fluorescence on the tegumental syncytiuni and cecal epithelium most significantly in positive sera, but negatively counter stained in negative sera. The use of the blood collected on filter paper showed simiiar results. Comparison of the sensitivity between the filter paper technique and the method using sera showed similar positive rates by IFAT. 142 out of 174 (81.6%) clonorchiasis sera were positive when the blood collected on filter paper were used; 140 out of 174 (80.3%) were positive when sera were used. The titers (log₂ units) of the four classified groups increased with the intensity of the infection by E.P.G.: 4.12 in light infection, 4.43 in moderate infection, 5.00 in heavy infection and 6.30 in very infection group. The relation of the titers (log₂ units) between the blood collected on filter paper(y) and sera(x) made the regression equation: y= 0.4x+1.85. showing relatively high correlation(r= 0.60. n = 52. p < 0.01).

분산 네트워크 관리 시스템 지원을 위한 멀티캐스트 프로토콜의 설계 및 구현

조현주,이광휘,김한수 國立昌原大學校 基礎科學硏究所 1994 基礎科學硏究所論文集 Vol.6 No.-

This paper presents a new model for group communication facility which is gradually magnified. This model has been implemented in LAN environment and can get the enhancement in performance because a new manager can be dynamically created determined depending on the type of receiving message. As an object oriented approach has been introduced in designing the system, it provides an application programmer with the means to develop a new application facility without having to build the detail functions. As the members in a group are only identified by the index in a table, the overhead for the address referencing can be minimized. In this paper, some requirements for multicast communication, the structure of the system, and service primitives will be describe in detail. As the number of manager can be dynamically created and destroyed relying on the requirement, the system can efficeintly operated. Multicast facility designed in this paper is for supporting a distributed network management system.

정신지체 학생의 구강실태 및 우식활성도에 관한 연구

한수경,김남순,조홍규,송호준,최충호,최남기 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

본 연구의 목적은 정신지체 학생의 구강건강 실태 및 치아우식 활성도를 평가하여, 학생들의 구강건강 증진을 위해 필요한 교육 방안을 모색하고, 실제적인 구강건강 관리 향상을 위한 기초 자료를 마련하고자 하는 것이었다. 광주광역시 소재 S 정신지체특수학교 유아부부터 고등부까지의 학생을 대상으로 조사를 실시하였다. 구강건강실태를 평가하기 위한 구강검사는 213명에 대해 시행하였으며, 치아우식활성도 검사는 197명에 대해 시행하였다. 치아우식활성도 평가를 시행한 197명의 정신지체 학생 중 우식활성이 고도인 학생은 33명(16.77%)으로 나타났으며, 13세 이하가 그 이상의 연령에 비해 치아우식활성도가 높았다. 치아우식활성도의 경중도와 치은염 유무와는 관련성이 없는 것으로 나타났다. 정신지체 장애학생의 치아우식활성도 경중과 치아우식경험지수와의 상관성을 평가한 결과, 치아우식활성도가 증가할수록 우식경험치율과 우식경험치지수가 높은 양상을 보였으며, 특히 유치의 경우 우식경험유치율 및 우식경험유치지수는 치아우식활성도와 높은 상관성을 보였다. The purpose of this study is to evaluate oral health status, caries-inducing bacterial activity of the mental retardation students, and developing motivation method for improving oral hygiene management by clarifying the relationship between caries- inducing bacterial activity and oral health status of mental retardation students. Caries experience indices, caries susceptibility test, gingival health evaluation, and oral hygiene management and oral health survey were performed. Results were as follows: 1. The DMFT index of disabled students(12 years) was 2.07. 2. The gingival inflammation was occurred more frequently in older ages. 3. There also was a high positive correlation between caries incidences and the results of caries-inducing bacterial activity test especially in deciduous teeth. This result suggested that the Dentocult SM mutans test as a caries activity test is a reliable method for measuring the status of dental caries in mental retardation students. Because it would motivate the mental retardation students to care more actively for their oral hygiene if they learn how much dental caries-inducing bacteria are living in their mouth by observing the bacterial colony on the cultured test strip, it can become a possibly efficient educational tool for the mental retardation students.

레미콘 타설 작업 중 발생한 피부 궤양 1례

조수헌,한상환 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.1

The case was described of a 43 year old male who developed burns of the both shins as a result of handling wet cement. After wet cement was spilled into the rubber boots he was wearing, he experienced painful erythema of both lower legs. Several weeks later, medical examination revealed dirty and granulated ulceration of the both shins. The largest ulcer, about 18 by 10 centimeters, was located on the anterior aspect of left lower leg. We concluded that this skin problem resulted from the extreme alkalinity of calcium hydroxide in wet cement, combined with the effects of pressure and occlusion against the skin.

임상재료에서 분리된 녹농균(綠膿菌)의 혈청형과 약제감수성(藥劑感受性)에 대하여

조양자,한옥수,이동후 대한미생물학회 1976 大韓微生物學會誌 Vol.11 No.1

Two hundred and fourty eight strains of Pseudomonas aeruginosa isolated from clinical materials at Department of Bacteriology in National Medical Center and Han-iI Hospital during January to November in 1973, were typed serologically by Hommos agglutination method utlizing a routine set of 13 standard sera. In addition, their susceptiblity to several kinds of antibiotics were determind. The following results were obtained; One hundred seventy eight strains (71,77%) were typable with an occurrence of type T5 in 41 strains (16. 58%), type T5, in 36 (14.52%), type T3, in 24 strains (9. 68%) and smalI numbers of strains were distributed in other types. Seventy strains (28. 23%) were nontypable. The rate of isolation of Pseudomonas by clinical meterials was shown as 49. 19% in ous, 16. 53% in sputum and 8. 87% in urine; the isolation rate of 1. 21-3. 15% was shown in other clinical meteraIs and the definite distribution rate could not be observed in the serotype by different materials. Majorities of strains used in this experiment of isolates were resistant to common antibiotics but Gentamycin and Carbenicillin, known relativeIy as sensitive antibiotics to Pseudomonas aeruginosa, were observed resistance of 2. 44-10. 5% and 16. 69-57. 8%. Moreover any particular relationship between serotype and the sensitivity of antibiotics was not identified.

가스크로마토그래피를 이용한 요중 마뇨산 측정시 새로운 시료 전처리 방법의 검증

조수현,임용현,김선민,권호장,하미나,한상환 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.1

Urinary hippuric acid was analyzed by gas chromatography/mass spectrometry method. A simple and economical derivatization procedure using excessive methanol in acidic condition was adopted to esterify hippuric acid. Quantitative analysis was performed with two internal standards, heptadecanoic acid and hydrocinnamic acid, to evaluate the derivatization procedure as an analytical method. Using the standard addition method, linear and reproducible calibration curves were obtained for both internal standards. Calibration experiment showed that good quantitative result could also be obtained with the calibration curve obtained without urine matrix. Recovery rates for the urine samples were in the range of 95.6∼111.4%. Using hydrocinnamic acid as internal standard, analysis time could be reduced.

유기용제 사용 소규모사업장의 체계적 건강관리 프로그램 개발에 관한 연구1) : 신경행동학적 검사법의 적용 가능성 Application of Neurobehavioral Core Test Battery(NCTB)

한상환,하미나,권호장,윤덕로,조수헌 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.2

It is known that there appear some nonspecific and delicate changes in neuropsychiatric aspect on the workers when they are exposed for a long period to low concentrated organic solvents in the work places. Therefore, it gives rise to the necessity of developing a program for the health supervisor stationed in the work place to supervise these neurobehavioral health affection. In addition to the questionnaire posed to the workers exposed to organic solvents, the study team conducted NCTB(Neurobehavioral Core Test Battery) respectively on an exposed group and a non-exposed group to organic solvents. The study team compared the results and decided whether or not to apply the results to the evaluation program of health affection. The study team divided 132 workers in 6 car repairing factories and 2 textile printing factories into exposed group and non-exposed group, pairing them off into 66 research objects according to their age, sex, and job. Neurobehavioral test was conducted on 6 items, except the POMS test, of NCTB developed by the World Health Organization(WHO). The study team conducted examinations of working environment on the exposed and non-exposed groups of each work place. The analysis of the collected materials for examinations were conducted with GC-MS. The concentration of the mixture in the air, relative to their respective threshold limit values(TLV) was calculated according to the formula of the ACGIH and the Korean threshold limits. The results indicated that for two of eight factories samples the recommended concentration limits for mixtures was exceeded. Of the six tests, the 'digit span forward', 'digit span backward' and 'digit symbol' produced conspicuous statistical differences between the two groups(p<0.1) as a result of compairing the test values by means of paired t-test. The results of respective analysis of the car repairing factories and textile printing factories revealed that the textile printing factories had greater differences between the exposed and the non-exposed than in the car repairing factories. It is presumably because the textile printing workers are continuously exposed to the organic solvents during working hours, whereas the car repairing workers are exposed only when they are doing painting works, The NCTB is assessed very useful in examining the neurobehavioral health affection under the exposure to organic solvents, and, therefore, the study team expects that the NCTB will play an important part in the course of developing a health program for the workers exposed to organic solvents.

LQ서보제어기를 이용한 천정크레인 시스템의 위치 제어

조영호,이한석,신승권,안두수 成均館大學校 科學技術硏究所 1998 論文集 Vol.49 No.1

An overhead crane is commonly used in factories and dockyards. An overhead crane operation is manually accomplished by skilled operators. Usually, the skill of the operators is relied on to bring the load to its destination exactly and have it stationary at the end of the journey. Recently, the concept of automation is widely introduced in shipping and unloading operation using the overhead crane for the enhanced producity. In this paper, we reduced overhead cranes scale and objection for experimental purpose in lab. The controller for an overhead crane was designed using LQ-servo technicques and simulated by MATLAB. The performance of the controller was verified through the experiment results and the simulation walls by MATLAB.