Effects of Phenytoin and Diazepam on the Seizure Activity in the Cortical Dysplasia Animal Models

Kim, Si-Hyung,Choi, In-Sun,Cho, Jin-Hwa,Park, Eun-Ju,Jang, Il-Sung,Choi, Byung-Ju,Kim, Hyun-Jung,Kim, Young-Jin,Nam, Soon-Hyeun The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injucted control animals. Field potential recordings were used to monitor amplitude and numbers population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5mg/kg;phenytoin 40, 60mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

조혈모세포이식 환자에서 침습성 진균 감염에 대한 Micafungin의 예방 효과 및 안전성

김시현,이동건,최수미,권재철,박선희,최정현,유진홍,이성은,조병식,김유진,이석,김희제,민창기,조석구,김동욱,이종욱,민우성,박종원 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: Micafungin, a potent inhibitor of 1,3-β-D-glucan synthase, is a novel antifungal agent of the echinocandin class. In vitro study showed that micafungin was effective against Aspergillus species as well as Candida species, but clinical data on the prophylactic efficacy against invasive fungal infections (IFIs) other than candidiasis are still lacking. Materials and Methods: We identified 60 consecutive adult hematopoietic stem cell transplantation (HSCT) recipients who received at least 3 doses of micafungin during neutropenic period. Micafungin was started as an alternative in patients who were intolerant or had adverse events (AEs) to primary prophylactic antifungal agents. We retrospectively reviewed the medical records and analyzed the efficacy and safety of micafungin for prophylaxis against IFIs. Results: The patients either had autologous (n=9) or allogeneic (n=51: 1 syngeneic, 24 sibling, 26 unrelated donor) HSCT. Itraconazole oral solution (n=58) was the most frequently used first line antifungal agent for prophylaxis and was administered for median 11 days. The most frequent cause of switch to micafungin was vomiting (n=42). The duration of neutropenia and micafungin administration was median 13 and 12 days, respectively. A successful outcome was achieved in 45 (75%) patients. Empirical antifungal therapy was initiated in 13 (22%) patients. There were 2 cases (3.3%) of breakthrough fungal infections which comprised a probable invasive pulmonary aspergillosis and a possible invasive fungal sinusitis. There was no case of invasive candidiasis. A total of 53 (88%) patients experienced at least one AE regardless of causality during micafungin administration. The most frequent AEs were hypokalemia, vomiting, diarrhea, and elevated serum aspartate aminotransferase or alanine aminotransferase. Among the aforementioned AEs, only 1 case of diarrhea could be classified as a probable relation with micafungin when causality was assessed. There was no AEs that caused discontinuation of micafungin. Conclusions: Micafungin seems to be a safe and effective agent for prophylaxis of IFIs including aspergillosis as well as candidiasis in HSCT recipients. However, further large, prospective, and randomized comparative studies are warranted for aspergillosis.

수원 일월저수지에서 Microcystis aeruginosa (K$\ddot{u}$tzing) K$\ddot{u}$tzing 수화현상 시 환경요인들의 변화

김지은,박정원,조기안,김시균,Kim, Ji-Eun,Park, Jung-Won,Jo, Ki-An,Kim, Si-Kyoon 한국하천호수학회 2013 생태와 환경 Vol.46 No.2

본 연구는 수원 일월저수지에서 수화현상 시 환경요인들의 변화와 한국형 부영양화 지수를 이용하여 영양단계를 평가하였다. 수화현상 종은 남조류의 Microcystis aeruginosa Kutz.였으며 조사기간 내내 우점하였다. 수온은 평균 $25.7{\sim}28.4^{\circ}C$, pH는 8.4~11.17, CODcr는 4.25~$72.0mg\;L^{-1}$, 전기전도도는 333.1~$749.0{\mu}S\;cm^{-1}$, Chl-a는 22.0~$185.0mg\;L^{-1}$였다. 정체 수역인 정점 2에서 CODcr는 다른 정점에 비해 2.9배 정도로 높아 내부기원 유기물의 기여도가 높은 것으로 판단된다. 영양염인 TN은 평균 28.86~$56.75mg\;L^{-1}$, TP는 0.2~$1.24mg\;L^{-1}$로 매우 높았고, Fe는 0.11~$1.05mg\;L^{-1}$이었고, Si는 3.13~$7.46mg\;L^{-1}$이었다. TN과 TP는 일월저수지 내에 지속적으로 누적된 것으로 파악되었고, Fe도 높은 농도가 유지되었다. 한국형 부영양화지수는 37.19~147.22였다. 영양단계는 중영양에서 과영양 단계로 평가되었고, 같은 저수지 내에서도 시기 및 지점에 따라 영양단계가 다름을 나타내므로 많은 자료가 추적된다면 한국형 영양단계 분석은 저수지의 영양단계 분석 시 매우 유용하게 활용될 것으로 판단된다. Variances in environmental factors were followed in Ilwol reservoir, Suwon, during bloom of Microcystis aeruginosa (K$\ddot{u}$tzing) K$\ddot{u}$tzing, Cyanophtya from August to October, 2011. M. aeruginosa dominated the water column throughout the investigation period. The water temperature varied from $25.7^{\circ}C$ to $28.4^{\circ}C$, pH 8.40 to 11.17, CODcr 4.25 to $72.00mg\;L^{-1}$, electrical conductivity 333.1 to $749.0{\mu}g\;cm^{-1}$, and Chl-a 22 to $185mg\;L^{-1}$. In particular, the high levels of CODcr is likely indicate high contribution of autochthonous organic matters in the reservoir. TN varied from 28.86 to $56.75mg\;L^{-1}$, TP 0.20 to $1.24mg\;L^{-1}$, Fe 0.11 to $1.05mg\;L^{-1}$, and Si 3.13 to $7.46mg\;L^{-1}$. These increases imply constant accumulation in Ilwol reservior, and reinforce the idea of autochthonous organic matters input in the reservoir. The Korea Trophic Status Index ($TSI_KO$) varied from 37.19 to 147.22. Trophic levels varied from mesotrophic to hypertrophic level, and differed spatio-temporally. Therefore, it is concluded that $TSI_KO$ is useful for analyzing trophic status of reservoirs.

요하지통 환자에서 두 개의 캐눌라를 이용한 박동성 고주파 신경근 응고술의 임상 연구

김천숙,배재영,배덕구,강규식,안기량,권진형,김지은,유시현 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Dorsal root ganglion(DRG) block by the local anesthetics and steroids which has done classically in low back pain(LBP) patients with radiculopathy has high incidence of recurrence rate and side effects of steroids. Recently a new technique of pulsed radiofrequency thermocoagulation(P-RFTC) was introduced and substituted for it because of the benefits such as relatively low recurrence rate, low risk of nerve injury and comfort during the procedure. We experienced 15 patients who had LBP with radiculopathy and no or little response to epidural steroid injection. When we performed DR ganglionotomy by P- RFTC under the C-arm guide in these patients, we tried to approach the exact DR ganglion using tow cannulas - the one for obtaining patient's subjective symptoms and injection of contrast dye, the other for making a RF lesioning after identifying the impedance of sensory and motor stimulation. In conclusion, DR ganglionotomy by P- RFTC using two cannulas in LBP patients is an easy and safe procedure with satisfactory results.

반코마이신 골시멘트의 유효성 및 안정성에 대한 고찰

황시영,김은영 충남대학교 약학대학 의약품개발연구소 2012 藥學論文集 Vol.27 No.-

Infections of prosthetic joints represent a major cause of morbidity from both the loss of mobility. Vancomycin impregnated cement is used as a spacer or during reimplantation surgery for the treatment of prosthetic joint infection. The aim for this article is to evaluate the effectiveness and safety for preclusive use of vancomycin to prosthetic joint. PubMed and EMBASE search was conducted using a combination sear ch term "vancomycin" as MeSH terms, "cement" as All Fields, and "infection" as All Fields. 9 out of 40 papers which were searched were used to reviewing. Effectiveness- although results of randomized, controlled trials were insufficient, the result shows that vancomycin-impregnated cement takes advantage of prevention and control of infection and maintenance of joint function. Safety- in some cases, nephrotoxicity was reported when vancomycin-impregnated cement was inserted. But still in needs more well designed RCTs and supportive guidelines. Although the use of vancomycin with impregnated cement is not approved by KFDA, it was effective and safe for the treatment of prosthetic joint infection based on this review.

당 함유 식품 섭취 증가가 어린이 건강에 미치는 영향에 관한 연구

김시경,황은선,김건희 덕성여자대학교 자연과학연구소 2007 자연과학 논문집 Vol.13 No.-

우리나라는 지난 20년간 설탕소비량이 무려 20% 가까이 증가했고, 간식으로 인한 설탕 섭취량 증가는 아동의 식생활을 변화시킨 중요한 요인이 되었음에 불구하고 우리나라 아동의 설탕섭취에 대한 연구는 매우 미흡한 실정이다. 식품에 첨가되는 설탕은 열량만을 낼 뿐 그 외의 영양소는 들어 있지 않으므로 "빈(empty) 영양소"라고 불리며, 많이 섭취하게 되면 상대적으로 영양소가 풍부한 다른 식품의 섭취가 적어져 영양불균형 상태를 초래할 수도 있다. 설탕으로부터 손쉽게 얻는 과도한 열량은 체지방으로 쉽게 축척되어 1차적으로 비만을 야기할 뿐 아니라, 당뇨병, 고지혈증, 충치, 과잉활동장애와 같은 2차적 문제들에 대한 심각성이 보고되고 있다. 우리나라 당류 소비현황과 어린이 기호식품의 당함량 실태에 대한 조사 결과를 바탕으로 문제점 분석 및 향후 발전 방향을 제시하고자 한다. 현재 우리나라 가공식품의 총당(total sugars) 함량표시는 의무화되어 있지는 않으며 과다한 당 섭취를 막고 과잉의 당 섭취로 인한 비만 등의 질병을 예방하기 위해서 총당(total sugars) 함량 표시는 시급하다. 어린이 및 청소년의 건강관리, 비만예방을 위한 당 함량 및 영양성분표시의 의무화가 필요하며, 어린이 기호 식품에 대한 정보를 어린이가 이해하기 쉽게 표기하여야 한다. 나아가 어린이는 올바른 식습관을 형성하는 시기인 만큼 바람직한 정책제시와 올바른 교육을 통하여 무분별한 당 섭취로 인한 어린이의 건강의 문제발생을 예방하고자 한다.

수술실 오염도감소를 위한 수술실공조시스템에 대한 연구

김천숙,박은정,강규식,김지은,안기량,권진형,유시현,주영철,권순정 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Background: The OR(operating room) should provide an optimum environment that is safe for the patient and the working personnel. The air ventilation system of OR has been studied to decrease the contamination of air. We investigated the flow and contamination of the air in OR at our hospital. Method: Cultures in OR were made and the number of bacteria and fungi were calculated. A two-dimensional model for the cross-section of an operation room is developed for the CFD (computational fluid dynamics) analysis. The characteristics of air flow in the empty operation room and in the occupied operation room are calculated by using a CFD program. Results: The current air ventilation system of our hospital does not deliver the clean air to the operating site efficiently in occupied OR. Conclusions: We suggested a new location of air ventilation system which improves air venting with little increase of the cost of equipment.

VoIP(Voice over IP)서비스 품질관리 방안 연구

노시춘,김태은 남서울대학교 2009 남서울대학교 논문집 Vol.15 No.2

VoIP service is divided into H.323 related service and SIP related service. Development of multimedia service for VoIP is in the process going beyond the utilization phase of voice service. This research proposes that VoIP QoS can be provided by managing the weakness of VoIP related protocol with design of measurement management process model for VoIP QoS. Furthermore, it proposes the use of analysed item and quality item driven per voice service from VoIP service quality measurement value of the site utilizing VoIP service can increase the quality. This research is finding the meaning and assignment of VoIP QoS to deduct how to manage the control system and presenting the QoS control process and trial framework. The trial framework is the modeling of the QoS measurement metrics, the measurement time schedule, the section, hierarchy, instrument, equipment and method of measurement and the series of cycle & the methodology about analysis of the result of measurement. Measurement result can be used for improvement of VoIP service quality of both service provider and user. However, if end user wants to measure the overall quality, not only IP network to be measured but also the Gateway. To acquire quality of VoIP, continuos development of efficient measurement method and measurement item to clearly indicate the quality level of service and performance of network, and applying of such development is necessary. Keywords : VoIP, QoS, framework

춘천 지역 주부들의 제례음식 준비에 관한 연구

김은실,함승시 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.4

The survey on the practice of the memorial ceremonial food in Chuncheon area showed it varied according to social position of officiator, location(inland or seaside town), and personal condition. The study included the foods prepared for the memorial services on the memorial day, New Year's Day and Chusok. 1. 40,4% of the subjects were in the thirties at their age, 46.6% were high school graduates, 57.3% were the first daughter-in-law, 40.4% had no religion, 30,9% were working at public administration and earned less than 1 to 1,5 million won monthly, 2. 71.7% of the subjects who replied that the ancestor worship service had to be kept were Buddhists, 55.4% of them were high school graduates, and 58.8% of them ran independent businesses. They learned how to practice the ancestor worship service from their mother before marriage or from their parents-in-law after marriage. 3. The older the officiators, the better they wanted to keep the traditional format of the service, but the Christians and Catholics wanted to change the format to western style in the future. 4. 92.7% of them served cooked milled rice. They prepared the soup in the order of beef soup, radish soup and dried Alaskan Pollack soup, 5. Among cooked vegetable dishes, bracken was used the most and balloonflower root, mung bean sprout and spinach followed, Among jeon(pan-fried foods), frozen Alaskan Pollack was used the most and buckwheat, mung bean and meatball followed. 6. They served san-juk(beef kebab) mostly on the ceremony. Among the grilled foods, tofu was the favorite, and croaker followed. 7. Among the fried foods, squid was the favorite, and sweet-potato and shrimp followed. Among the dried foods, they used in the order of dried Alaskan pollack, dried beef and squid. 8. Among the rice cake and traditional confectionery, they used in the order of Yak-sik(sweet rice cake), Gang-jeong(fried glutinous rice cookie), Jeol-pyun and In-jeol-mee. Among a beverage, they served Sik-hye(fermented rice drink) mostly. 9. Among fruits, apples, jujube, chestnut and dried persimmon were served. Among a liquor, Cheongju was served mostly. 10. Soy sauce, salt and salted fermented fish were served, too.

류마티스성 승모판 협착증의 치료를 위한 경피적 승모판 성형술의 치료성적

김성은,조흥근,박성훈,박시훈 梨花女子大學校 醫科大學 醫科學硏究所 1999 EMJ (Ewha medical journal) Vol.22 No.4

연구목적 : 류마티스성 승모판 협착증에 대한 유용한 치료법인 경피적 승모판 성형술의 본원의 단기 치료 성적을 알아보고자 하였다. 방법: 1993년 10월에서 1999년 4월까지의 이대목동병원에 내원한 류마티스성 승모판 협착증 환자 21명에서 투시조명 하에 경피적 승모판 성형술을 시행하였다. 시술전에 경흥부심초음파와 경식도초음파가 시행되었고, 시술후에는 경흉부초음파를 사용하여 혈역학적, 임상학적 변화를 비교하였다. 연구대상의 거의 모두가 NYHA class Ⅱ에 속했고, 5명이 심방세동을 가지고 있었으며, 모두 심초음파점수가 8이하였다. 좌심방에 혈전이 있는 경우로 혈전을 용해시킨 후 시술한 경우가 2예였다. 결과 : 경피적 승모판 성형술 이후의 평균 승모판구면적은 시술전의 1.16±0.36㎠에서 2.06±0.33㎠로 증가되었다. 경승모판 이완기 평균 압력차는 시술전 11.60±5.54mmHg에서 시술 후 4.93±2.53mmHg(p<0.001). 좌심방 크기는 46.41±14.66mm에서 42.03±15.01mm로(p=0.042), 그리고 심박출량은 4.21±1.25L/min에서 6.88±9.57L/min로(p<0.0001) 의미있는 호전을 보였다. 3도 이상의 심한 승모관폐쇄부전이나 심각한 시술후 합병증은 없었다. 결론 : 본원에서 약 6년간 시행된 경피적 승모판 성형술는 그 사례가 적고 시술전 예상되는 난이도 면에서 특이한 것은 없었지만, 시술후 합병증이 거의 발견되지 않았으며 혈역학적 측정상에서도 성공적인 시술을 보여주었다. 승모판구면적에 따른 시술후 단기내 호전의 정도는 거의 예측할 수 없었으나, 심초음파점수는 모두가 8점이하로 높은 성공율의 조건을 제공하였다. Objective : Percutaneous mitral valvuloplasty(PMV) became a treatment modality or mitral stenosis because of its low morbidity, short hospital stay, and low cost. We reviewed clinical and hemodynamic results after PMV for the patients with mitral stenosis in Ewha Womans University Mokdong hospital. Methods : We compared the results of echocardiographic, hemodynamic, and clinical parameters before and after PMV. PMV was performed under fluoroscopic guidance in 21 patients(M:4, F:17, mean age 43±12 years) with mitral stenosis from October 1993 to April 1999. Transesophageal echocardiography(TEE) and Transthoracic echocardiography(TTE) were performed for the evaluation of mitral valve, chamber size, and the presence of left atrial thrombus before procedures. TIE was also used for follow-up evaluation. On presentation, all patients showed at least NYHA class Ⅱ. Five patients had atrial fibrillation. Two patients with thrombus in the left atrium were included to study group after thrombolytic treatment with coumadin. Echo-score of our patients was not greater than 8. Results : Mean mitral valve area(MVA) by 2 dimensional or Doppler echocardiography was increased from 1.16±0.36㎠ before PMV to 2.06±0.33㎠ after PMV. There were marked improvements in transmitral gradients(11.60±5.54mmHg before PMV vs 4.93±2.53mmHg after PMV, p<0.001), left atrial dimension(46.41±14.66mm vs 42.03±15.01mm, p=0.042), and cardiac output(4.21±1.25L/min vs 6.88±9.57L/min, p<0.0001) following PMV, Severe(≥GⅢ) mitral insufficiency or severe postprocedural complications were not noted. This suggested that all procedure was successful. Conclusion : The Procedural success rate of PMV in Ewha Womans University Mokdong hospital was 100%. Low echo score of our patients might explain this high procedural success rate. Long-term-follow-up is warranted in the near future.