Chiral Separation of (±)-Higenamine by Capillary Electophoresis

Choi,One-Kyun,Jung,Kyo-Soon,Choi,Heisook-Yun,Yang,Deok-Chun 한국자원식물학회 2003 Plant Resources Vol.6 No.1

Higenamine [1-(4-hydroxy-6, 7-dihydroxy-l, 2, 3, 4-tetrahydroisoquinoline) is a cardiotonic constituent of Aconiti tuber, one of the most widely prescribed oriental medicines. S-(-)higenamine was reported to have a stronger cardiotonic activity than R-(+)-higenamine and known as a central intermediate in the biosynthesis of various benzyl isoquionoline alkaloids in plants. The separation of higenamine enantiomers has been accomplished with capillary electrophoresis using cyclodextrins (CDs) as chiral selectors. Good resolution of this enantiomers was obtained using a 50 mM sodium phosphate buffer containing hydroxypropyl β-CDs using 27 cm fused silica capillary (50㎛ i.d., 20 cm to detector) at 25℃. With the electric field of 340 V/cm, the separation time of higenamine enantiomers was less than 6 min. Under this optimum conditions, the relative standard deviations of migration time and peak area were less than 1.6% and 3.2%. A 512-channel diode array detector was confirmed for the higenamine. The detection limits (S/N = 3) of these enantiomers are 1.5mutextrmm/mL. We confirmed the chiral form of higenamine in medicinal plants.

복막투석복막염 환자에서 발생한 창자벽공기증 1예

정선영,나지훈,최윤정,고성애,조규향,박종원,도준영,윤경우 영남대학교 의과대학 2009 Yeungnam University Journal of Medicine Vol.26 No.1

Peritonitis is a serious problem in patients undergoing peritoneal dialysis. Rarely pneumatosis intestinalis can occur as a complication of this infectious process. Pneumatosis intestinalis is a potential life threatening condition with a challenging management. The mortality of peritoneal dialysis patients with pneumatosis intestinalis secondary to mesenteric ischemia is almost 100%. We describe a rare case of pneumatosis intestinalis in a peritoneal dialysis patient who developed Staphylococcus aureus peritonitis which was initially treated with appropriate antibiotics. Since initial response to therapy was not achieved, an abdominal computerized tomography was done which revealed a pneumatosis intestinalis. A laparotomy was performed and small bowel necrosis was seen. A segmented resection with ileostomy, jejunostomy was done. Though surgical treatment was performed, the patient died in 2 weeks after admission. Pneumocystitis intestinalis in peritoneal dialysis peritonitis is a uncommon complication which requires prompt evaluation to rule out mesenteric ischemia as it carries a high mortality and its management will be surgical.

식민지 이후의 탈식민주의 -설정식 시를 중심으로-

최윤정 ( Yun Jung Choi ) 한민족문화학회 2012 한민족문화연구 Vol.39 No.-

설정식은 한때 월북문인이라는 표식에 갇혀 삭제되거나 좌익적 경향의 시인으로 리얼리즘이나 문학가동맹과 연계되어 민족의 현실을 간과하지 않았다는 사실만으로 평가되었다. 그의 시론으로부터 혁명적 낭만주의 경향을 포착하게 되는데, 이는 민족주의적 현실인식의 관점이 제공되는 계기를 드러낸다. 우리는 설정식이 해방공간의 현실을 깊게 들여다보고 당시의 좌우 이데올로기에 편향되기 보다는 그 안에 내포되어 있는 제국주의를 간파하고 두 진영으로부터 거리를 두려했던 민족주의자로 파악할 수 있게 되는 것이다. 우선 우편향적 미국에 대해서는 ``인민``이라는 계급이데올로기를 표방함으로써 미국에 결탁한 보수세력들과 미국적 제국주의와 거리를 두게 되고, 그 ``인민``이 혈통적 단위임을 피력함으로써 계급적으로 구체화되는 존재가 아니라 민족적 주체들임을 제기하고 민족을 무시하는 국제주의 이데올로기를 표방하는 소련적 사회주의와도 거리를 두게 된다. 또한 ``자유``라는 민주주의의 이상이 파괴되는 현실은 미국의 식량정책으로 표면화된다. 쌀 기근 사태는 아직도 이 땅에서 나는 곡식의 주인이 될 수 없는 현실을 드러내며, 식량정책을 주도하는 미국과 거리를 두게 하며, 추상적인 사상과 이데올로기를 표방하는 ``빵``과 대비됨으로써 민족의 현실을 구체화하게 된다. 이렇게 2장에서는 좌우이데올로기로부터 등거리를 유지하면서 거기에 내포된 제국주의를 간파하게 되고, 민족주의적 경향을 뚜렷하게 드러내는 과정을 통해 ``인민``, ``자유``, ``민족``이 설정식 시텍스트의 키워드임을 발견한다. 3장에서는 ``인민``과 ``자유``, ``민족``을 드러내는 대립적 상징체를 통해 민족담론을 구체화하게 된다. ``인민``은 2장에서 혈통적 민족임이 드러난다. ``인민``의 상징체인 ``해바라기``는 제국의 상징체인 ``태양``을 필적하는 존재로 파악된다. ``자유``는 2장에서 민족적 속박의 지속성을 환기시키는데, 제국에 의해 민족이 분열된 영토는 ``집``으로 표기되고 제국이 도모하지 못하는 민족의 절대域을 ``숲``으로 대비함으로써 구속과 예속으로부터의 탈주를 통해 민족적 ``자유``를 예고한다. 또한 ``팔월``이라는 민족의 기념비적 시간을 통해서는 해방이 되었으나 아직 해방이 되지 않은 두 가지 현실 사이에 민족이 놓여 있음을 통해 진정한 해방이 되어야 함을 당위적으로 설파하게 된다. The time of liberation from Japaness imperialism that we are beyond the point we are met with the United States and the Soviet Union is a branch of a new empire. The japanophilism has not been liquidation and our nation stood at the crossroads. Our situation was anxiety in the time. Because A mix of colonialism and postcolonial hold, was present between the division and independence. In various trend, Seol Jeong-sik is only one configuration of the discourse. It was not yet complete will be liberated. The only one empire has been removed by another empire. Even after liberation, our colonial life was being sustained. This discourse is constantly activated, he is discarded pseudo-ethnic discourse divide into left-wing and right-wing ideology. And he presents nation discourse consists of the bottom. He was fully aware of the postcolonial. It is national independence and people`s independence is to be done with. In fact, the important problem was the restoration of nation territory. His anti-imperialist lines does not permit any empire. So he placed a distance equal to the Soviet Union and the United States. In this perspective, he will be close to nationalist rather than proletarian.

크론병에 대한 CoPPLX의 방어효과에 대한 연구

이호학,김유림,문창기,이재일,양병윤,김종수,이정민,정재혁,안석진,박상준,김윤권,김소연,김영중,조민구,최영자,장성조 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

성장기 쥐에서 이소플라본 첨가수준이 골밀도 및 골함량에 미치는 영향

최미자,정윤정 啓明大學校 生活科學硏究所 2005 科學論集 Vol.31 No.-

The purpose of this study was to examine the effects of isoflavones supplementation on bone mineral density and bone mineral content in growing female Sprague-Dawley rats. Twenty-seven rats were divided into three groups casein diet, casein+1/2IF(1.7 mg isoflavones/g protein)diet, and casein+IF(3.4mg isoflavones/g protein) diet. All rats has been fed on experimental diet and deionized water ad libitum for 9 weeks. Bone mineral density(BMD) and bone mineral content(BMC) were measured using PIXImus(GE Lunar Co, Wisconsin, USA.) in spine and femur on 9 weeks after feeding. Diet did not affect weight gain and mean food intake. Food efficiency ratio was lower in casein +IF groups. The casein+IF and casein+1/2IF group had significantly higher spine and femur bone mineral density than the casein group. We found that bone mineral density effected by isoflavones supplementation has been resulted differently in accordance with the type of bone. Therefore, isoflavones supplementation may be beneficial on spine and femur BMD increasement in growing female rats.

솔잎(갈비)·솔방울의 수처리에 관한 연구

최순규,엄기수,성현순,김윤영,이시우,정대일,이용균 동아대학교 환경문제연구소 1994 硏究報告 Vol.17 No.2

The absorption ability of low density heavy metal of pine needles and cones is better than one of existing absorption materials in treatment ability and experimental condition. The elimination ability of chlorine of pine needles and cones is better than one of active carbon. In elimination of trihalomethane, the effect of treatment of pine needles is lower than one of pine cones. And surface of pine needles and cones after absorption of heavy metal ion was observed by Scanning Electron Microscope(SEM). Application and availability of pine needles and cones as absorption material need lots of experiments. The experiment on Gal-Bi(fallen leaves) is proceeding.

人乳中의 高分子糖蛋白質에 관한 硏究

崔禎延,李守遠 成均館大學校 科學技術硏究所 1992 論文集 Vol.42 No.2

This study was carried out to compare the high molecular weight glycoprotein isolated from human milk fat globule membrane and whey, and to investigate biochemical or immunological properties of two materials. The results are summerized as follows : 1. The milk fat globule membrane (MFGM) of human milk contained about 15 proteins and 5∼6 major glycoproteins, while human whey contained about 10 proteins and 3∼4 major glycoproteins. 2. Human MFGM and whey contained a high molecular weight glycoprotein, termed PAS-O, which did not migrate in 10% SDS-acrylamide gel elecrophoresis. 3. PAS-O isolated by Sepharose CL-4B chromatography had maximum absorption at 208nm and no absorption at 280nm. 4. On the agglutination test with eight lectins, WGA, PNA, LPA reacted with PAS-O and HM-glycoprotein that suggested to act as surface receptor of them in human milk. 5. Serine, glycine, alanine and glutamic acid were predominant amino acids in the both high-Mr glycoprotein, PAS-O and HM-glycoprotein. On the other hand, sulfur containing amino acids were present in very small amount. 6. The patterns of reactivity of these two glycoproteins, PAS-O and HM-glycoproteins, with some lectins, the similarities in their amino acid compositions and electrophoretic mobilities, suggested that they may be the same molecules.

1-Phenylindole Ester류의 합성과 반응성 연구

崔舜圭,金兪瑛,兪丙國,李龍均,鄭大一 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

일반적인 dienophile에 대한 pyrrole의 반응은 pyrrole이 diene system으로 작용된 [4+2] 고리부가반응과 pyrrole의 α-탄소 위치에 부가되는 Michael형태의 반응이 알려져 있다.

새로운 Cyclic Oxalate의 반응성 연구

鄭大一,金侖瑛,崔舜圭,李龍均 東亞大學校 大學院 1994 大學院論文集 Vol.19 No.-

As a new protective group for diols, cyclic oxalates (6) (7) were synthesized by using oxalyl chloride, rthyl oxalyl chloride, diethyl oxalate and oxalic acid. Cyclic oxalates were readily cleaved to the corresponding diols by various bases(CH₃ONa/CH₃OH, K₂CO₃/CH₃OH, Et₃N/CH₂Cl₂1% NaOH/CH₂Cl₂, LiAlH₄/CH₂Cl₂) but were stable in acid(p-TsOH).

콘크리트 포장의 플라이애쉬 재활용 방안연구

정영화,최성용,윤경구,용석응,김태경 강원대학교 석재복합신소재제품연구센터 1998 석재연 논문집 Vol.3 No.-

한국 대부분의 고속도로는 콘크리트로 포장되고 있으며, 플라이애쉬를 포장용 콘크리트에서 재활용하면 콘크리트의 성능을 향상시킴과 동시에 경제적 이익과 환경보호에 큰 기여를 할 것이다. 본 연구에서는 플라이애쉬를 포장 콘크리트에 재활용하고자, 플라이애쉬 치환율, 물-시멘트비, 굵은골재 최대치수를 주요실험 변수로 하여 이들이 플라이애쉬를 혼입한 포장용 콘크리트의 역학적 특성에 미치는 영향을 살피기 위해서 실험하여 통계처리 분석하였다. 이러한 분석결과를 바탕으로 포장 콘크리트의 적정배합을 제시하였으며 결과는 다음과 같다. 플라이애쉬를 혼입한 포장콘크리트의 휨강도에 영향을 미치는 인자는 플라이애쉬 치환율이 가장 큰 것으로 나타났으며, 다음으로 굵은골재 최대치수와 물-시멘트비 순으로 영향을 미친다. 또한, 플라이애쉬 치환율과 굵은골재 최대치수의 상관관계가 가장 큰 것을 알 수 있었다. 목표압축강도를 목표 휨강도의 6배로 선택하여 적정구간을 결정하였으나, 적정배합구간이 목표압축강도에 의해서 결정되었다. 이는 플라이애쉬의 작은 수화물이 공극을 효과적으로 채움으로써 휨강도의 증진에 크게 기여하기 때문으로 판단된다. 고속도로 포장에 적용할 수 있는 32mm의 굵은골재 최대치수와 45%의 물-시멘트비를 사용하는 목표 휨강도 45kg/㎠의 적정배합에서는 5.0%까지 플라이애쉬를 치환 가능하고, 50kg/㎠의 적정배합에서는 2.5%까지 치환 가능하다. 공항 포장에 적용할 수 있는 40mm의 굵은골재 최대치수와 37%의 물-시멘트비를 사용하는 목표 휨강도 45kg/㎠의 적정배합에서는 4.5%까지 플라이애쉬를 치환 가능하고, 50kg/㎠의 적정배합에서는 2.0%까지 치환 가능하다. The most of highway in Korea have been paved by concrete since last ten years. The recycling of fly ash in pavement concrete may result in economics, the improvement of concrete quality and the reservation of environment. This paper focused on the series of experiment and statistics analysis with experimental variables such as fly ash substitute ratio, water-cement ratio and maximum size of coarse aggregate in order to investigate the feasibility of recycling of fly ash in pavement concrete, to see the strength characteristics and t propose the optimum mix design based on previous results. The results of experiments and analysis were follows; The fly ash substitute ratio affected most seriously to the flexural strength of concrete among main variables, followed by maximum size of coarse aggregate and water-cement ratio. The interaction between fly ash substitute ratio and coarse aggregate size was the biggest. The optimum mix design was determined by target compressive, which was selected as 6 times of flexural strength. This indicated that the small hydrate of fly ash in concrete filled in effectively and resulted in the enhancement of flexural strength. the optimum mix design for highway pavement concrete with 32mm of maximum size of coarse aggregate and 45% of W/C can incorporate up to 5.0% and 2.5% of fly-ash at 45 kg/cm² and 50 kg/cm² of target flexural strength, respectively. The optimum mix design for airport pavement concrete with 40mm of maximum size of coarse aggregate and 37% of W/C can incorporate up to 4.5% and 2.0% of fly ash at 45 kg/cm² and 50 kg/cm² of target flexural strength, respectively.