Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

백제시대 부여 지역의 산성과 석성산성

유원재 公州敎育大學 敎育硏究所 2011 公州敎大論叢 Vol.47 No.1

Buyeo was the capital city of Baek-je for 123 years until its fall on King Uija's 20th year reign after Baek-je had transferred its capital to Buyeo on the 16th year's reign of King Sung. Unlike Gongju limited in space, Buoyeo was economically opulent due to its geographical advantage of having mass fields and also suited for defense with it surrounded by the Geumgang. King Sung built a new town castle for the transfer of capital and equipped Buyeo with all sorts of facilities for defense of the capital city by constructing mountain castles and their outskirts. This research is aimed at taking a look at such a mountain castle in the period of Buyeo of Baekjei Kingdom. Mountain castles in Korea could be historical sites succeeding to its national uniqueness and tradition. The form of the mountain castles in Korea can be divided into two-a mountain-top-base castle and a castle including valleys- mostly by a criterion of locational conditions of a mountain castle and using methods of the topography of castle walls. Nevertheless, the period of Bakeje witnessed a new type of multiple-system mountain castles built by combining the existing two forms. In other words, as a means of expanding a small scale mountain-top-base castle into a larger one, the people in Baekjei did an additional construction by linking the a fortress encompassing valleys to the very adjoining topography, producing a new form of multiple system fortress combined by the existing two forms. Among these, Seokseong Fortress is an example in Buyeo district of the period of Baekje and this Seokseong Fortress assumed an important role in defens of a castle town in the Sabi period[ranging from King Sung's capital transfer to Buyeo to the fall of Baekje]. The spread of this multiple-type mountain castle mostly belongs to the territory of Baekje or is discovered at Baekje-related districts. It is believed that this new type of mountain castle was created by Baekje people's novel idea and is concluded as a new transformation of ancient mountain fortress in Korea that appeared in late period of Baekje. Therefore, the research on this multiple-type mountain fortress could be a big help to understanding the history of Baekje at that time, its local organization and social composition, which is considered to bring about much bigger development in research on Baekje history in its stagnant period. 부여는 성왕 16년 538년 공주에서 천도한 후 660년 멸망할 때 까지 백제의 수도이었다. 성왕은 천도를 위하여 새로운 도성을 건립하고 산성과 나성을 축조하여 도성방어를 위한 제반시설을 갖추었다. 본고에서는 이러한 백제, 부여 시대의 산성에 대하여 알아보고자 한다. 한국의 산성은 산성이 위치한 입지적 조건과 성벽의 통과선이 구체적으로 지형에 대한 이용방법을 기준으로 하여 산정식 산성과 포곡식 산성의 두 형식으로 나눌 수 있다. 그러나 백제, 부여시대에 이 두 형식을 결합한 새로운 형식의 복합식 산성이 백제시대 산성의 한 형태로 축조되었다. 따라서 본고에서는 백제시대의 부여지역의 산성과 복합식 산성인 석성산성에 대하여 알아보고자 한다.

통풍형 방음벽 개발 및 적용에 관한 연구

윤제원(Je-Won Yoon),김영찬(Young-Chan Kim),김금모(Keum-Mo Kim),장강석(Kang-Seok Jang),구본성(Bon-Sung Ku),엄주용(Joo-Yong Eom) 한국소음진동공학회 2011 한국소음진동공학회 학술대회논문집 Vol.2011 No.10

The purpose of this study is to develop an air-passing soundproofing panel with more improved structure to reduce the CO2 emission and installation cost. To reduce the emission of CO2 ; it is suggested to choose low CO2 emission material relative to the aluminum and to reduce the materials by developing a specially designed air-passing soundproofing panel structure. First of all, we performed the flow analysis to predict the wind pressure according to the open angle of the air-passing soundproofing panel and the noise level analysis at the receiver point. To verify the simulation, a prototype of the soundproofing panel was made. The flow test in the wind tunnel and load test were performed. The economic evaluation for the installation of the air-passing soundproofing panel was performed and specifications of the installation was prepared. As the results of this research, it was verified that the wind load was reduced about 40% to that of the conventional one at 25m/s wind speed in the wind tunnel test. By applying the 4m span soundproofing wall with air-passing soundproofing panel and under the cost of 250 thousand won/m2 instead of the conventional 2m span panel, the installation cost will always be lowered than the conventional one in the combination of (60:40~50:50) conventional to air-passing soundproofing panel from the economic evaluation. The 20% reduction of CO2 was found by changing the 50% of aluminum soundproof panel to air-passing soundproofing panel.

조혈모세포이식술이 골수기질세포의 기원 및 조골세포로의 증식 및 분화에 미치는 효과

강무일,조성원,오은숙,백기현,이원영,오기원,김혜수,한제호,윤건호,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

Girder의 강성을 고려한 복합 재료 교량 상판의 구조 거동

원치문,박제선,이정호,심도식 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-

Many of the bidge and building floor systems, including the girders and cross-beams, also behave a similar special orthotropic plates. Such plates are subject to the concentrate masses in the form of traffic loads, or the test equipments such as the accelerator in addition to their own masses. Analysis of such problems is usually very difficult. Most of the bridge slabs on girders have large aspect ratios. Finite difference method is used for this purpose, in this paper. The reuslt is compared with that of the beam theory.

오픈케이슨기초의 침하촉진을 위한 AIR-JET 공법의 적용성 분석

박진원,류제천,임희대 忠南大學校 産業技術硏究所 2003 산업기술연구논문집 Vol.18 No.2

By air jet method, settlement time was much decreased and settlement was reached even to the depth which conventional method could not. The friction reduction increased more than 20 to 30%. In addition to this merits, air jet method can make caisson settlement without separating loading or increasing dead loads. By this method, size of caisson sections can be reduced for resulting in tremendous cost reduction. Also, caisson damage by blasting can be prevented with this method.

시멘트 콘크리트의 건조수축균열 특성

박제선,윤경구,이주형,김태환,정원경 강원대학교 부설 석재복합신소재 제품연구센터 2000 석재연 논문집 Vol.5 No.-

콘크리트는 본질적으로 인장강고와 휨강도가 약하며 취성적 성질을 나타내는 결점을 가지고 있으므로 이를 보강할 목적으로 콘크리트에 섬유를 혼입한 섬유보강 콘크리트가 크게 각광받고 있다. 특히 폴리프로필렌 섬유보강은 모르타르 및 콘크리트가 건조나 동해에 의해 수축될 때 구속에 의해 발생되는 인장응력 및 균열을 제어하고 인성의 증가와 충격, 마모, 피로에 대한 저항성, 내구성을 증대시키는 장점을 가지는 것으로 보고 되고 있다. 1축으로 구속된 건조수축의 구속으로 인한 조강형 콘크리트의 잔류응력을 선형모델을 이용하여 예측할 수 있었고 조강형 콘크리트에 섬유보강으로 인한 건조수축 제어효과는 일반큰코리트에 비해 효과가 매우 큰 것으로 나타났다. 따라서 폴리크로필렌 섬유의 혼입으로 인해 콘크리트의 균열제어가 가능하며, 균열 폭이 큰 균열보다는 많은 미세 균열을 유도하는 것을 알 수 있었다. 섬유보강 조강형 큰크리트의 동결융해저항특성은 거의 동해의 손상을 받지 않으며 좋은 내구성을 유지하고 있는 것으로 나타났다. 이는 불규칙하게 배향된 섬유가 공극수의 이동을 효과적으로 제어함으로써 메트릭스 내의 팽창압으로 인한 파괴를 최대한 줄였기 때문이라고 보여진다. 표면손상과 박리저항에 매우 우수하며 저항성을 증진시키는 효과가 뛰어남을 확인할 수 있었다. 따라서, 내구적 측면의 유지, 보수에 있어서도 섬유보강은 그 효과가 큰 것으로 나타났다. To improve the brittle nature of concrete, discrete fibers can be adapted in concrete. Since polypropylene fiber has many advantages in many points on its chemical stability and good durability, its usages have been increased gradually. It has been reported than polypropylene fibers can not only control restrained tensional stresses and cracks, but also increase toughness, resistance to impact, corrosion, fatigue, and durability. Although the polypropylene fiber reinforced concretes with ordinary cement have been studied so much, those with very early strength concretes have not studied at all. Therefore, it is necessary to initiate and conduct the researches for the polypropylene fiber reinforced very early strength concretes which has enough workability and durability. This study of polypropylene fiber reinforced very early strength concrete focused on the investigation each material's effect into strength development with analysis of restrained shrinkage properties with one-dimensional concrete members. The results showed that more optimized mixture combinations could be selected in very early strength concrete by incorporating polypropylene fibers, keeping in same strength levels at urgent repair works. The formation of needle-shape ettringite helps the early strength development, specially at flexural and this enables the repaired concrete structures or pavements to be opened to traffic after 3 hours of concrete placement. A residual stress of early strength concrete caused by one-dimensional restrained drying shrinkage was predicted from linear model. the control of shrinkage crack by fiber reinforcement at early strength concrete was better than that of ordinary portland cement concrete because of bridging effect of fiber at early age and resulting of more hair cracks rather than fewer wider cracks. As a results, this dissertation clearly validated the fiber reinforcement on the strength development and durability of polypropylene fiber reinforced very early strength concrete, and characteristic of restrained shrinkage. The proposed optimized concrete mixtures might be satisfactory applied at the repair works in fields, because of its durability and crack control capacity against freeze-thaw and contraction at early age, respectively.

우심방과 우심실의 거대 심장 혈관종 1예

김제열,조상호,김건일,허경림,김현숙,조구영,최영진,이원용,임종윤 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Cardiac tumors, especially the primary tumors involving any part of the heart are extremely rare and its relative incidence has been reported to be approximately 0.02%. We report a patient with huge cardiac hemangioma who complained of shortness of breath, general weakness, and dizziness, Imaging study by echocardiography and computed tomography of thorax revealed a huge lobulating mass like a bunch of grapes in the right chamber of heart. The surgical exploration of thorax was performed and a histological diagnosis of spindle cell hemangioma was obtained by microscopy. The patient was treated by surgical resection of the tumor and doing well after surgery. Our experience indicated that prompt diagnosis and treatment of cardiac hemangioma is imperative for patients' prognosis.

鹿茸藥鍼이 骨形成에 미치는 影響

한상원,최제용,이윤호 대한침구학회 2001 대한침구의학회지 Vol.18 No.5

Objective : Bone homeostasis is maintained by balance of bone formation and resorption. Therefore, bone related diseases arose by disturbance of this balance between osteoblast and osteoclast activities. To develop a successful screening system of the therapeutic components based on oriental medicine is essential to set out systematic approach for that purpose. Methods : This study was perforated Sprague-Dawley rat femur for bony defects(φ 5㎜) by the fissure bur. And experimetal group were treated with Cervi Pantotricuhum Cornu injection at both She`nshu~(BL23) & Da`zhu`(BL11)(0.2㎖). This was evaluated by radiography and histological analysis with in situ hybridization. Results : Cervi Pantotricuhum Cornu Herbal Acupuncture has weak effect on bony defect healing and this was evaluated by X-ray taking and histological analysis with in situ hybridization. Osteocalcin gene expression was not changed by Cervi Pantotricuhum Cornu Herbal Acupuncture in bony defects animal model. Conclusion : Taken together this study show that the Cervi Pantotricuhum Cornu herbal-acupuncture has a weak effect healing of bony defects, this type of approach might give a good chance to explore the favorable effects of Cervi Pantotricuhum Cornu herbal-acupuncture on bone tissue.

野冬靑果가 實驗的 糖尿에 미치는 影響에 관한 免疫組織化學的 硏究

柳濟原,李學仁,金蓮燮 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

This experiment was performed into investigate the effect of Syzygii cumini Fructrs on the diabetic rats induced by STZ. Experimental diabetes was induced by 55㎎/㎏ of STZ. injection and administered Syzygii cumini Fructrs extract during 14days after STZ injection. Glucagon and insulin granules in Langerhans islets were stained by use of immunohistochemical method and observed the relative amount of the each granules in Langerhans islet by light microscope and image analysis ststem. The results obtained are as follows: 1. Area % of insulin granules in Langerhans islets in Syzygii cumini Fructrs raised and showed the statistically significant difference with the control group at 14th day. 2. Area % of glucagon granules in Langerhans islets in Syzygii cumini Fructrs decreased and showed the statistically significant difference with the control group at 7th and 14th day. To conclude, it can be inferred that Syzygii cumini Fructrs has a control effect on glucagon and insulin granules in Langerhans islets of diabetic rats induced by STZ.