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      • KCI우수등재

        한우 육성빈우 (育成牝牛) 의 에너지와 단백질요구량 추정 1 . 대사시험에 의한 한우 육성빈우의 에너지와 단백질 요구량 결정

        정연후(Y . H . Chung),이상철(S . C . Lee),강수원(S . W . Kang),정정수(C . S . Chung),정천용(C . Y . Chung) 한국축산학회 1992 한국축산학회지 Vol.34 No.5

        Metabolism trial was carried out to estimate the energy and protein requirements for Korean native heifers. Nine heifer calves weighing about 150㎏ were randomly assigned into three target daily gains (0.2㎏, T₁; 0.6㎏, T₂; 0.8㎏, T₃) with interval of BW 50㎏ through BW 350㎏, aiming to estimate the energy and protein requirements. The amount of nutrient to be fed was based on NRC requirements (1984), and the ration consisted of hay, rice straw and grass silage as roughage source, and concentrate mixture. Average daily gains across body weight classes were 0.30(T₁), 0.62(T₂) and 0.67㎏(T₃), respectively. Except for crude fiber, digestibilities and nutritive values of experimental ration were increased with the increase of daily gain. As expected, nitrogen intake was significantly(P$lt;0.05) increased with the increase of daily gain, but there were no differences in digestible nitrogen intake and nitrogen retention between T₂ and T₃ group. Based on pooled data of body weight classes, nitrogen(N) and digestible nitrogen(DN) requirements were estimated as follows; N requirement(g/W^0.75/day) = 0.98+1.25DG-0.44DG² (R²=0.42**), DN requirement(g/W^0.75/day) = 0.40+1.19DG-0.24DG² (R²=0.49**) Metabolizable energy intake, heat production and energy retention tended to increase with the increase of daily gains, not with body weight, although there was no difference between T₂ and T₃ group. Energy losses expressed as % of gross energy(GE) intake was higest from feces(42.8%), followed by heat production(42.3%), and lowest from urine(2.0%). Total digestible nutrient(TDN), DE and ME requirements were as follows; TDN requirement(g/W^0.75/day) = 28.13+60.40DG-14.03DG² (R²=0.48**), DE requirement(㎉/W^0.75/day) = 134.34+ 220.59DG-71.90DG² (R²=0.54**), ME requirement(㎉/W^0.75/day) = 116.98+171.32DG-35.73DG²(R²=0.54**). Total digestible nutrient, DE and ME requirements for maintenance were 28.13g, 134.3㎉ and 116.98 ㎉/W^0.75/day, respectively. The estimated NEm requirement from the relationship between ME intake and heat production was 76.5㎉/W^0.75/day.

      • Expression of mosquitocidal crystal protein genes in non-insecticidal Bacillus thuringiensis subsp. israelensis

        Gill, S.S.,Oh, H.W.,Lee, D.W.,Roh, J.Y.,Park, H.W.,Jin, B.R.,Je, Y.H.,Kang, S.K. 東亞大學校附設遺傳工學硏究所 1998 遺傳工學硏究 Vol.- No.5

        J.Y.ROH,H.W.PARK,Y.H.JE,D.W.LEE,B.R.JIN, H.W.OH,S.S.GILL AND S.K.KANG.1997. Bacillus thuringiensis NTB-1 isolated from soil samples in Korea produces ovoidal parasporal inclusions with proteins of approximately 24-40kDa in size. Although serological study indicated that the isolate has a flagella (H) antigen identical with subsp. israelensis, it seemed to be non-insecticidal against Lepidoptera and Coleoptera as well as Diptera. To investigate the activity of non-insecticidal B.thuringiensis transformed with insecticidal crystal protein genes, cryIVD and cytA genes of B. thuringiensis subsp. morrisoni PG-14, highly toxic to mosquito larvae, were introduced into the isolate NTB-1. The expression of mosquitocidal crystal protein genes in NTB-1 was characterized by SDS-PAGE analysis and electron microscopy. The results showed that crystalline inclusions of host, CryIVD and CytA were stably expressed in the transformant. However, the mosquitocidal activity of transformant was similar to that of B. thuringiensis subsp. kurstaki Cry-B harbouring cryIVD and cytA genes, demonstrating that a synergistic effect by an interaction of both introduced insecticidal and resident non-insecticidal crystal proteins was not observed.

      • KCI등재

        Development of Naturally Degradable “Rice Polymer" For Organic Weed Management of Red Pepper and Rice

        C.K. Kang,H.S. Nam,Y.K. Lee,S.B. Lee,B.M. Lee,Y.J. Oh,H.J. Jee,M.K. Hong,K.W. Jung,Y.J. Lee,Y.H. Choi 한국유기농업학회 2011 韓國有機農業學會誌 Vol.19 No.S

        Among the developed bío-degradable polymer fílms as compared wíth transparent fílm(Whíte), black polymer fílm was sígnífícantly more effectíve for controllíng weeds ín red pepper. Also, we found that whíte and black polymer mulchíng had 81.8% and 97.9% of managing weed controllíng effects in rice, respectively. Compared to nonmulched rice paddy with water supply, the non-mulched rice paddy wíthout any water supply has stopped its growth at 41 days after transplantíng, whíle polymer -mulched rice paddy without water supply had about 60% of normally growing rice plants. This shows the polymer treatment has a remarkable effect on water and power saving, solution of herbicidal resistance, avoidance of herbícidal influence to eco-system etc. When the naturally decomposíng polymer was used, a temperature was elevated as high as 4.7 ℃ on maximum and 2.6 ℃ on average. Also the naturally decomposing polymer accelerated rooting by 7 days and lowered a stress level from transplanting. The weed control effect mulched by polymer was remarkable as 98. 7%. The p이ymer now, after 294 days treated on the rice paddy, has been completely decomposed.

      • KCI등재

        LIGA-like 공정을 이용한 마이크로 부품 복제용 Ni과 Ni-W 금형 제조 및 특성

        황완식,박준식,강영철,조진우,박순섭,이인규,강성군,Hwang, W.S.,Park, J.S.,Kang, Y.C.,Cho, J.W.,Park, S.S.,Lee, I.G.,Kang, S.G. 한국재료학회 2003 한국재료학회지 Vol.13 No.1

        Electroplated Ni and Ni-W micro-molds using LIGA-like process for replication of micro-components such as microfluidic parts and micro optical parts have been investigated. In general, it is hard to produce micro-parts using conventional mechanical processes. Micro-mold formed by LIGA-like process could fabricate micro-parts with high aspect ratio. In this paper, fabrication and properties of electroplated Ni molds with varying applied current types as well as those of Ni-W molds were investigated. Ni molds fabricated under pulse-reverse current showed the highest hardness value of about 160 Hv. Ni-W molds showed the hardness of about 500 Hv which was much harder than that of Ni electroplated molds. The above results suggested that high quality micro-molds could be fabricated by using Ni electroplating of pulse-reverse type for core molds and sequential Ni-W alloys coating.

      • 터키種 잎담배 生産에 關한 硏究

        盧載榮,鄭元采,安長憲,卞珠燮,姜信寓 충북대학교 엽연초연구소 1974 煙草硏究 Vol.2 No.-

        I.Influence of deficient or excessive supply of soil moisture. The variety used in this study were Turkish varieties (Smyrna, Samsun, Zichna and Xanthi). Soil moisture content were 30%, 45% and 60% to maximum water-holding capacity for whole growth period. This experiment was carried out to study on the influence of soil moisture to growth and physiology of Turkish varieties. The results are summarized as follow ; a. Dry weight of Samsun and Zichna were-increased at 45% soil moisture content, but Smyrna and Xanthi at 60%. In every soil moisture treatmennt, there were significant difference between D plot and M, W plot but there were no significant difference between M plot and W plot. b. Percentage of dry matter of Smyrna was higher then those of the other varieties in the D plot and W plot. c. Leaf area was increased ill the Samsun of which process was slightly late. In every soil moisture treatment, M plot and W plot were higher than D plot. d. Dry weight per unit leaf area of Smyrna and Zichna were higher than that of Xanthi and Samsun. e. Percentage of dried up leaves was the W plot, and lower at the D and M plot. Smyrna was lower at M plot, Zichna at D and M plot, Xanthi at D plot, but Samsun was no difference among plots. f. In the number of harvested leaves, Smyrna was increased in order W>M>D. In the number of harvested leaves, Smyrna showed difference according to the soil moisture in order W>M>D plot, but Samsun and Zichna showed no difference among each treatments, Xanthi was higher in M plot than D and W plot. From those results, Smyrna showed the highest dry weight on account of the weight of leaf area and percentage of dry weight increased, it was decreased in order Zichna>Samsun Xanthi in the each soil moisture contents. Number of harvested leaves, dried up leaves, leaf area and number of harvested leaves but decreased, percentage of dry matter W treatment increased the number of total leaves, number of dried up leaves, leaf area, percentage of dried up leaves and percentage of dry matter. Yields were the lowest at D treated, there were no difference between M and W treatment. II.Influence of deficient or excessive supply of soil moisture at different growing stages. This experiment was carried out to study on the influence to the growth and physiology according to the soil moisture content during each growth stage on Turkish varieties culture. The varieties used in this study were Samsun, Zichna and Xanthi. The results are summarized as follow; a. Dry leaf weight was decreased in order Zichna>Samsun>Xanthi, there were remakable difference among every variety. In each soil moisture content, weight of dry matter was increased by M and W treated at late stage after MW and MM treated at early and middle stage of growth DWM and MDM plot. Dry leaf weight was decreased at D plot more than M and W plot late stage after early stage was treated by MW and MM. Dry leaf weight was the highest at WWM treate, dthere were high significant difference between varieties and soil moisture contents. b. Percentage of dry matter was decreased in order Zichna=Xanthi>Samsun. In soil moisture treated, it was increased in D treated at the latter stage after MM, MW and WM treated at early and middle stage, but it was decreased by DDM, DWD, MDM, WWD, WMM, and WWM treated. There were high significant interaction between varieties and soil moisture contents. c. Leaf area showed in order Samsun=Zichna>Xanthi. In soil moisture treated, leaf area was increased by WM, MM and MW treated at the middle and latter stage after D treated at early stage, but it was decreased at WWD, WMD, WWM, MWD and MMD treated. d. Dry weight per unit leaf area were no difference among varieties, in soil moisture treated, it was increased by the MMW, MWD, MMD, WMD and DMW treated, weight of per unit leaf area showed significant difference interaction between varieties and soil moisture contents. d. Percentage of dried up leaves showed as follow, Samsun>Xanthi, Samsun=Zichna, Zihna=Xanthi. In soil moisture treated, it was increased by M and W treated at early stage than D treated. f. Number of harvested leaves was decreased in order Xanthi>Samsun>Zichna, there were no difference among soil moisture contents, but appeard high significant difference at the interaction between varieties and soil moisture contents. As the results of this experiment, especially, this experiment will be continue on the influence of soil moisture condition in order to development of home production system.

      • 1987년 한국에서 발생한 렙토스피라병의 혈청역학적 조사

        이증훈,박영수,이우곤,김석용,정선식,우준희,박성광,박경희,송영욱,김선영,기정일,최두혁,강성귀,김주완,최강원,김우열,최명식,최인학,장우현,윤성열 대한감염학회 1988 감염 Vol.20 No.3

        Human leptospirosis was an unfamiliar disease in Korea until 1984 that outbreak of leptospirosis occurred among farmers and soldiers after field works for harvesting rice. During that time, Lee and Jo confirmed the first Korean cases of leptospirosis by serological test, isolation of causative agent and autopy findings. Afterward several outbreaks occurred also during autumn especially after flood in every years and some characterisitcs of leptospirosis in Korea such as clinical manifestations, serotypes and seroepidemiological features has been revealed by many investigators. Because of the major mode of transmission between rodents and human is by direct contact with leptospiral urine of rodents or contaminated soil by the urine, leptospirosis in Korea has been primarily a disease of person in occupations heavily exposed to contaminated soil or infected urine such as farmer, army and etc. Therefore it seems that leptospirosis is one of the main communicable diseases to be controlled urgently in Korea, for an agricultural people account for almost half of total Korean people. For clarifying the seroepidemiological patterns of human leptospirosis in Korea by sex, month region and main reacting serovars of L. interrogans among acute febrile disease occurred in 1987, 1,773 patient's sers with acute febrile episodes were tested by microagglutination test using 19 representative strains of leptospiral serogroup as antigen. All of those sera were collected from 10 collaborative clinics located in Kyunggi, Kangwon, Chungbuk, Chungnam, Chonbuk, Chonnam province and Seoul. The results wee summerized as follows. 1) Among 1,773 sera of patients with acute febrile episodes, 219 (12.4%) were seropositive to L. interrogans, 487(27.5%) to R. tsutsugamushi, 241(13.6%) to R.typhi and 160(90.0%) to Hantaan virus. 2) Among seropositives to L.interrogans, the male outnumbered the female, 65% and 35%. 3) For age distribution, 26.9% of seropositives to L.interrogans were fifties, 19.6% were forties, 9.1% were sixties, 5.9% were thirties and 4.1% were twenties. 4) Eighty three percent of seropositives had occurred between September and October in 1987 with a peak in September. 5) Main leptospiral serovars reactive to patient's sera were Icterohaemorrhagiae(54.3%), Canicola(31.0%), CH-48(13.2%), Tarassovi(0.9%)and Cynopteri(0.5%). 6) For regional distribution, 65.8% of seropositives to L.interrogans were residents from Chonbuk, 12.3% were Chonnam, 7.3% were Chungnam, 5.5% were Kyunggi and 1.4% were Kangwon.

      • SCISCIESCOPUS

        Long-term outdoor cultivation by perfusing spent medium for biodiesel production from Chlorella minutissima

        Oh, S.H.,Kwon, M.C.,Choi, W.Y.,Seo, Y.C.,Kim, G.B.,Kang, D.H.,Lee, S.Y.,Lee, H.Y. Society for Bioscience and Bioengineering, Japan ; 2010 Journal of bioscience and bioengineering Vol.110 No.2

        A unique perfusion process was developed to maintain high concentrations of marine alga, Chlorella minutissima. This method is based on recycling cells by continuous feeding with warm spent sea water from nuclear power plants, which has very similar properties as sea water. A temperature of at least 30 <SUP>o</SUP>C in a 200 L photo-bioreactor was maintained in this system by perfusion of the thermal plume for 80 days in the coldest season. The maximum cell concentration and total lipid content was 8.3 g-dry wt./L and 23.2 %, w/w, respectively, under mixotrophic conditions. Lipid production was found to be due to a partially or non-growth related process, which implies that large amounts of biomass are needed for a high accumulation of lipids within the cells. At perfusion rates greater than 1.5 L/h, the temperature of the medium inside the reactor was around 30 <SUP>o</SUP>C, which was optimal for cell growth. For this system, a perfusion rate of 2.8 L/h was determined to be optimal for maintaining rapid cell growth and lipid production during outdoor cultivation. It was absolutely necessary to maintain the appropriate perfusion rate so that the medium temperature was optimal for cell growth. In addition, the lipids produced using this process were shown to be feasible for biodiesel production since the lipid composition of C. minutissima grown under these conditions consisted of 17 % (w/w) of C<SUB>16</SUB> and 47% (w/w) of C<SUB>18</SUB>. The combined results of this study clearly demonstrated that the discharged energy of the thermal plume could be reused to cultivate marine alga by maintaining a relatively constant temperature in an outdoor photo-bioreactor without the need for supplying any extra energy, which could allow for cheap production of biodiesel from waste energy.

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