군자산 자생 망개나무 임분의 구조적 특성

이경수 ( Kyung Su Lee ),정태영 ( Tae Young Jung ),박철하 ( Chull Ha Park ),한주환 ( Ju Hwan Han ),이귀용 ( Gui Yong Lee ),구창덕 ( Chang Duck Koo ) 한국산림과학회 2012 한국산림과학회지 Vol.101 No.4

Berchemia berchemiaefolia is a rare and endangered species and important as a honey resource. The purposes of this study were to investigate physical environmental factors and the population and community structure of Berchemia berchemiaefolia stands at Mt.Gunja in Sogrisan National Park in Chungcheongbuk-do. The B berchemiaefolia stands belonged to deciduous forests in mid-temperate zone and were 5~25˚ in slope 30~90% in rock ratio and 6.37 in soil pH. They had 656 trees within the area of 36 ha and their average density was 18.2 trees per ha. The trees within the stands were 11.8m in tree height 22.6 cm in DBH and 56 years old. The number of branched stems was the maximum 8 and 32.9% of the trees were branched. B. berchemiaefolia trees over 12cm diameter occupied 89.9% but young B. berchemiaefolia did only 11%. The structure of B. berchemiaefolia stands were classified into three groups as Acer pseudo-sieboldianum & A. mono community group A. pseudo-sieboldianum & Quercus aliena community group and B. berchemiaefolia & Quercus serata community group. The mean importance value of B. berchemiaefolia was 10.9% while species diversity of the community ranged 0.77~1.31 and interspecific competition ranged 0.78~0.94. The dominance of the community ranged of 0.07~0.29 and the evenness ranged 0.71~0.93. Although B. berchemiaefolia was dominant in the habitat but the species was already in natural successional stage to cornus controversa or Ulmus davidiana var. japonica.

敎養體育 敎科課程에 있어서의 學習者의 要求와 反應 : 女子大學을 中心으로

河泰卿 聖心女子大學校 1969 論文集 Vol.1 No.-

비기능성 부신피질암 1례

하달봉,이경섭,손형규,이준희,원유홍,김동훈,장태정 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

비기능성 부신피질암은 발생 빈도가 극히 드문 악성 종양이며, 내분비학적 임상증상이 없어 주로 복부 종물에 의해 우연히 발견된다. 그래서 대부분 종양의 크기가 거대하고 전이로 진행된 경우가 흔하며, 절제술만으로는 치유 불가능한 경우가 많아 예후가 불량한다. 최근 저자들은 약 10년간 간헐적인 좌측 측복통과 흉부 압박감으로 내원하여, 이학적 검사 및 방사선학적 검사상 복부 종물이 확인되고 생화학적 검사에서 비기능성으로 판명된 52세 남자 환자에서 근치적 신절제술을 시행하여 병리조직검사상 비기능성 부신피질암으로 진단되었기에 문헌 고찰과 함께 보고하는 바이다. Nonfunctioning adrenocortical carcinoma is extremely rare, which is diagnosed most often in older man because of mass effect. Most tumors are discovered too late for curative resection, and advanced tumors are associated with a poor prognosis. Herein we report on a 52-year-old man with nonfunctioning adrenocortical carcinoma, treated by radical nephrectomy.

보중익기탕합인진사령산이 CCl₄中毒 흰쥐의 肝損傷에 미치는 影響

河智容,白泰鉉,張梗植 대한동의병리학회 1997 동의생리병리학회지 Vol.11 No.1

간병변은 현대인들의 스트레스, 과로, 음주, 고지방식의 과다 섭취 등의 각종 원인에 의하여 점차 증가하고 있는 실정이다. 최근 이에 따라 간기능을 보조하는 드링크류나 약재가 개발되어 시판되고 있으나, 아직까지 뚜렷한 효능을 가진 약재가 없는 현실이다. 간질환이 발병시 피로, 노권, 식용부진, 황달, 복수, 발열, 심와부통(心窩部痛), 구토, 소변황적 등의 증상을 호소하는데 이를 변증하여 보면 허중협실(虛中狹實) 즉 허증인 중기부족증(中氣不足症)과 실증인 비위습열증(碑胃濕熱症)이 혼재되어 있음을 알수 있다. 이에 본 저자는 부정거사(扶正祛邪)가 간질환 치료에 효과적일 것으로 보고 보중익기탕(補中益氣湯)과 인진사령산(茵蔯四笭散)을 합방하여 CCI₄로 간손상을 유발한 백서(白鼠)의 투여하여 간기능회복여부를 연구하고자 하였다. 군을 정상군, CCI₄처리군, CCI₄처리후약재투여 A군(10㎎/㎏), CCI₄처리후약재투여 B군(20㎎/㎏)으로 나누어 일정기간 약재를 투여한후 채혈하여 AST, ALT, Bilirubin, ALP, γ-GT, LDH, Albumin, total cholesterol, α-Fetoprotein의 함량을 측정하였다. AST, γ-GT, α-Fetoprotein의 경우는 A, B군에서 대조군에 비하여 감소하였으나 A군에서는 유의성이 인정되지 않았고, ALT의 경우는 A, B군 모두에서 유의성있는 감소를 보였으며, Bilirubin의 경우 A, B군 모두 대조군에 비하여 증가하는 경향을 보였으며, ALP의 경우 A군에서는 감소하였으나 B군의 경우 오히려 증가하는 경향을 보였다. LDH의 경우 A, B군 모두 감소하였으나 유의성은 나타나지 않았으며 Albumin의 경우 A, B 모두 증가하는 경향은 보였으나 유의성은 없었다. total cholesterol의 경우 A, B군 모두 유의성있는 증가를 보였다. 이상의 결과를 종합하여 볼 경우 보중익기탕합인진사령산(補中益氣湯合茵蔯四笭散)은 간손상 회복에 영향을 미치는 것으로 보여지며 회복기전에 관련된 연구는 더욱 진행되어야 할것으로 보여진다. This paper is to investigate what effects Bojungikkitanghapinjinsaryungsan-treated A-group(10㎎/㎏ treated group) and Bojungikkitang-hapinjinsaryungsan-treated B-group(20㎎/㎏ treated group) have on ratliver injured by Ccl₄, So this experimental research is focused on measuring about AST, ALT, bilirubin, ALP,γ-GT, LDH, albumin, TP, and α-Fetoprotein. The results are summarized as follow; 1. As to AST(GOT), Bojungikkitanghapinjinsaryungsan-treated A-group was slightly decreased with no effectiveness, but Bojungikkitan-ghapin-jinsaryungsan-treated B-group was decreased with effectiveness, as compared with the control group. 2. As to ALT(GPT) Bojungikkitanghapinjinsaryungsan-treated A-group and Bojungikkitanghapinjinsaryungsan-treated B-group were decreased with effectiveness, as compared with the control group. 3. As to bilirubin, Bojungikkitanghapinjinsaryungsan-treated A-group Bojungikkitanghapinjinsaryungsan-treated B-group were increased, as compared with the control group. 4. As to ALP, Bojungikkitanghapinjinsaryungsan-treated A-group was slightly decreased with no effectiveness, but Bojungikkitanghapinjinsaryungsan-treated B-group was slightly increased. 5. As to γ-GT, Bojungikkitanghapinjinsaryungsan-treated A-group was slightly decreased with no effectiveness, but Bojungikkitanghapinjinsaryungsan-treated B-group was decreased with effectiveness, as compared with the control group. 6. As to LDH, Bojungikkitanghapinjinsaryungsan-treated A-group and Bojungikkitanghapinjinsaryungsan-treated B-group were slightly decreased with no effectiveness, as compared with the control group. 7. As to albumin, Bojungikkitanghapinjinsaryungsan-treated A-group and Bojungikkitanghapinjinsaryungsan-treated B-group were slightly increased with no effectiveness, as compared with the control group. 8. As to TP, Bojungikkitanghapinjinsaryungsan-treated A-group and Bojungikkitanghapinjinsaryungsan-treated B-group were increased with effectiveness, as compared with the control group. 9. As to α-Fetoprotein, Bojungikkitanghapinjinsaryungsan-treated A-group was slightly decreased with no effectiveness, but Bojungikkitanghapinjinsaryungsan-treated B-group was decreased with effectiveness, as compared with the control group. According to the above result, it could be suggested that Bojungikkitanghapinjinsaryungsan have prominent improvement on the liver disease.

인체 혈장에서 염산시프로플록사신(시프로플록사신으로서 250㎎) 정량을 위한 HPLC 분석법의 유효성검토

하용화,조성희,천성국,서성훈,류재환,최영욱,이경태 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.4

A simple HPLC method with ultraviolet detection of ciprofloxacin in human plasma was developed and validated. After protein precipitation with trichloroacetic acid, chromatographic separation of ciprofloxacin in plasma was achieved at 50℃ with a C_18 column and methanol-phosphate mixture (pH 2.5), as mobile phase. Quantitative determination was performed by ultraviolet detection at 278 nm. The method was specific and validated with a limit of quantification of 100 ng/ml. The infra- and inter-day coefficients of variation were between 1.67% and 10.55% and accuracy between 92.01% and 106.09%. The method has been successfully applied in a bioavailability study of 250 ㎎ ciprofloxacin hydrochloride tablet.

PCS측정 기술과 Kozeny-Carman모델을 이용한 여과공정 특성연구

하상안,김승호,윤태경 한국환경과학회 2004 한국환경과학회지 Vol.13 No.9

The filtration tests were made in cell with a low concentrated suspension. The suspension with a concentration of c_(M) = 1.14~2.67 · 10^(-3) gig consists of paper paint and water. The particles in the suspension have a particle size x<l㎛. The used depth filters consists of glass fibres, which are coated by polymer. The filtration in depth filters accorded in different mechanism, which were explained by physical models. The model which would be allows to make a promise of the filtration reaction. This filter media allows to get a high filtration time and a good separation rate. The Particle distribution is measured by a photon correlation spectroscopy(PCS). PCS measures particle sizes 0.03 ㎛<d<3 ㎛ in the suspension. The filtered suspension has a very low concentration Co ≤ 5x10^(-4) gig of solid in sample. The PCS also informs us about the number of the particles in the suspension. The makes it possible to calculate the concentration of the in sample.

락탐화합물의 고리 확장 연구 : 입체 선택성과 Mechanism에 관한 연구 Study of Stereoselectivity and Mechanism

하진렬,최경민,김지아,원태진,신동수,이민주,안철진 昌原大學校 基礎科學硏究所 2002 基礎科學硏究所論文集 Vol.14 No.-

The ring expansion chemistry is one of the most important methods to prepare five-and six-membered heterocylic compounds. We have studied about γ-lactam ring expansion with stereoselectivity from β-lactam derivative. To do this chemistry, proton of N-benzyl group in β-lactam derivatives is eliminated by LDA and make carbanion.This anion make unstable 4-membered lactam intermediate, and then the ring is opened by homo clevage or hetero cleavage for releasing ring strain, followed by recyclization to one carbon to make γ-lactam ring with stereoselectivity in β-, γ- positions.

열교환기 구조 변화에 따른 NH₃와 R22의 성능특성연구

하옥남,하경수,이승재,정송태 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

Nowadays HCFCs refrigerant are restricted because it cause depletion of ozone layer. However, natural gases such as ammonia as an organic compound, propane and propylene as hydrocarbon are easy and cheap to obtain as well as environmental. Accordingly, this experiment apply the NH3 and R22 to study the performance characteristic from the superheat control and compare the energy efficiency of two refrigerants from the high performance. The condensing pressure of refrigeration system is increased from 15bar to 16bar and degree of superheat is increased from 0 to 10℃ at each condensing pressure. As the result of experiment, when comparing the each COP, we knew the NH₃ is suitable as the alternative refrigerant of the R22.

모듈화된 계층의 구조를 갖는 시뮬레이션 모델에서의 모델간 연결 방법론과 그 적용 예

하경재,정민수,조대호 慶南大學校 附設 基礎科學硏究所 1995 硏究論文集 Vol.7 No.-

대량의 지식베이스를 처리하는 시스템의 모델링을 하기 위해서는 큰 규모의 데이터베이스에 저장되어 있는 정보를 효과적으로 접근할 수 있어야 한다. 본 논문에서는 데이터가 계층의 구조를 갖고 모듈화 되어 있는 능동적인 기억소자(Processor/memory pair)에 저장되어 있는 시스템의 모델링에 사용할 수 있는 단계적접근(level access)방법론을 어떻게 유사추론 시스템(analogical reasoning system)에 적용 할 수 있는가를 보여준다.

단계적 온도 하강법을 이용한 췌도세포 냉동보존법

정인경,오승훈,김병준,양태영,이병완,하창영,노정현,정재훈,민용기,이명식,이문규,김광원 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.1

연구배경:최근 당뇨병의 새로운 치료법으로 시도되고 있는 췌도이식은 충분한 췌도수의 확보와 췌도생존율을 높이기 위한 면역억제제 사용이 제한점이 되고 있다. 본 연구의 목적은 이식 전 충분한 췌도 수의 확보를 위해 분리한 췌도를 냉동보존하는 방법을 확립하고 냉동보존한 백서 췌도세포의 시험관내 그리고 생체내 기능을 조사하였다. 방법:분리한 백서의 췌장소도를 48시간 배양한 후 한 시험관당 췌도세포 1000개씩 나누었다. 냉동보존은 6개의 시험관에 DMSO를 첨가한 후 초 냉각(supercooling), 핵화(nucleation)단계를 거친 후 99% isopropanol과 액체질소가 들어있는 dewer를 이용하여-0.25℃/분의 냉각속도로 -40℃까지 단계적으로 얼린후-70℃ 액체질소 탱크에 보관했다. 해동은 냉동시킨 vial들을 액체 질소 태으에서 꺼내 37℃ 항온조에 담가 급격히 해동시킨 후, 원심분리하여 상층액을 제거하고 각 vial에 0.75M sucrose 용액을 가한 후, 10% fetal calf serum이 함유된 RPMI 1640 media에서 배양하였다 각각 6개의 시험관에서 해동한 췌도들을 광학현미경 및 형광현미경하에서 췌도의 모양과 생존율에 대해 조사하고 인슐린 정적반응을 알아보았다. 또한 분리한 췌도를 냉동보존하지 않고 이식한 경우를 대조군(6마리)과 생체내 기능을 비교하였다. 결과:① 냉동보존후 획득한 췌도의 수와 생존율 해동후 획득한 췌도의 수는 해동시킨 당일날이 902±21, 24시간 배양 후에는 857±16, 72시간 후에는 817±18개로 점차 감소되었다. AO/PI 염색상 각 췌도의 생존율은 냉동 전을 100으로 하였을 때 해동당일, 24시간 후, 72시간 후가 각각 60±5, 80±5, 90±5%로 해동후 3일간 배양하였을 때 냉동전의 수준으로 회복하였다. ② 냉동보존후 췌도의 포도당에 대한 정적 인슐린 분비능:냉동직후 감소된 경향을 보였으나 해동후 3일간 배양한 췌도의 인슐린 분비는 냉동전과 통계적으로 의미있는 차이가 없이 냉동보존 전의 수준으로 회복되었다. ③ 냉동보존후 췌도의 포도당에 대한 동적 인슐린 분비능:냉동보존한 췌도를 해동후 3일째의 인슐린 동적 분비능은 냉동 전과 마찬가지로 자극 인슐린의 반응의 제1기와 2기가 잘 관찰되었다. ④ 냉동보존한 췌도세포 이식 후 혈당 변화:스트렙토조토신으로 유도된 당뇨병 쥐에 췌도이식 후 혈당은 냉동보존한 췌도이식군에서 대조군에 비해 혈당의 조절효과가 더 오래 지속되었다. 결론:소동물에서 단계적 온도 하강법을 이용한 췌도세포 냉동보존법을 확립하였으며 이는 기능, 구조 및 생존율에 큰 이상을 보이지 않았으므로 장차 사람의 췌도세포 동종이식시 부족한 췌도세포수를 극복하고 면역반응을 줄일 수 있는 매우 유용한 방법이 될 것으로 판단된다. Background : Although islet transplantation has been attempted to reverse the state of diabetes, achieving a critical number of islets and modulating the immune response limit the success of islet transplantation. Cryo-preservation of islets offers many important benefits for islet transplantation by collecting islets with a wide variety of HLA phenotypes and islet MHC expression. The aims of this study was to determine the optimal conditions for cryo-preservation by using a controlled cooling method and to evaluate in vitro and in vivo functional properties of the cryo-preserved islets. Methods : Collagenase-isolated, Ficoll-purified islets were cultured for 48 hours. They were aliquoted into freezing tubes (1000 islets per tube), equilibrated with 2M dimethyl sulfoxide (DMSO) in three steps, supercooled, nucleated, and controll-cooled at rate of 0.25℃/min to - 40℃ prior to storage at - 196℃. Rapid thawing and removal of DMSO with 0.75 M sucrose preceded 48 hour of culture and the morphology, viability, glucose-induced insulin secretion, and in vivo function of rats transplanted with cryopreserved islets was reexamined. Results : ① Recovery was 90.2±0.2%, 85.7±0.1% and 81.7±0.1% immendiately after, 24 hours and 72 hours after thawing respectively. The viability was 60±5%, 80±5%, 90±5% immediately after, 24 hours and 72 hours after thawing respectively. ② The glucose-stimulated-insulin secretion (GSIS) tended to decrease immediately after thawing, but GSIS increase to the level of pre-cryopreservation 72 hours after thawing. ③ The in dynamic GSIS, the first and the second phase of insulin secretion were well preserved in islets cultured for 72 hours after thawing. ④ The cryopreserved islets were cultured for 3 days and transplanted into renal sub-capsular space of streptozotocin (STZ) induced diabetic rats. The duration of normoglycemia in the STZ-induced diabetic rats transplanted with cryopreserved islets was significantly longer than of the fresh islets. Conclusion : The optimal condition of cryopreservation using the controlled cooling method was established in rat pancreatic islets. This cryopreservation method can be a feasible approach for human islet transplantation (J Kor Diabetes Asso 26:64~74, 2002).