생물전극 반응조를 이용한 영양물질 제거

김성완,윤철종,최성우,김성우,우성훈,신남철,박승조 동아대학교 환경문제연구소 1999 硏究報告 Vol.22 No.2

We have investigated a performance of bio-electrode reactor for removal of nutrient like a nitrogen, phosphorus and organic substrate. Lab scale of bio-electrode reactor was operated with synthetic and tannery wastewater. Iron bar and stainless steel used for anode and cathode respectively. In experiment with synthetic wastewater, we were able to obtain the optimal current density range of 2.4-40 mA/dm² after 48 hrs operating time. And in that experiment, about 70~73% of ammonia nitrogen and 54~64% of phosphorus were removed. In experiment with tannery wastewater at 2.4-4.0 mA/dm², the removal rate of ammonia nitrogen, phosphorus and organic substract were about 62-69%, 45~59% and beyond 79% respectively.

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

2형 당뇨 환자에 있어서 C형 간염의 유병률에 관한 연구

김구영,서광섭,유광렬,임병욱,채선애,강남훈,김창훈,권영우,김희수,류성태,이정환,박성우,오웅식,김정한 중앙대학교 의과대학 의과학연구소 2003 中央醫大誌 Vol.28 No.1

Background: We have doubt about the truth that Type 2 DM is associated with chronic hepatitis C infection. The aim of our study was to prove a close relationship between chronic HCV infection and type 2 DM in korean patients. Methods: We reviewed the out-patient's medical records of 466 patients with type 2 DM who visit the Sung-ae hospital from March 2000 to August 2001. Diabetic patients were analyzed for the following variables: age, sex, waist circumference, weight, waist/hip ratio, using of self monitoring of blood glucose, method of therapy, duration of disease, experience of previous transfusion, major surgical procedure, accompanied disease, liver function tests (LFT), HbA1 c. We ruled out patients with liver cirrhosis. Results: Hepatitis C virus infection was observed more frequently in patient with Type 2 DM. Of 466 Type 2 DM patients, 13 patients were infected with Hepatitis C virus (2.79%). Compared with mean prevalence of Korean population (1.3~1.6%), it's value is very high. the other hand, 21 patients were infected with Hepatitis B virus (4.5%). Such results are similar to mean prevalence of the general public (4~5%). Conclusions: Prevalence of hepatitis C was closely associated in korean patients with type 2 DM. And an increased prevalence is independent of cirrhosis.

동등백분위법을 이용한 순발력 검사 방법간의 동등화 방안

남덕현,윤상화,김도준,박재우 龍仁大學校 體育科學硏究所 1999 體育科學硏究論叢 Vol.9 No.1

This paper is designed to equalize the points obtained from different tests of agility by means of equipercentile equating in accordance with grade and sex. The subjects consist of 923 high school students who have ever gone through the tests of power. As a result, I have reached the following conclusion. 1. I used equipercentile equating for recording the tests of power of the first male graders, coming up with equalized values such as 223㎝ in a standing board jump, 33.7m in five seconds' running and 42m in throwing, and 53m in a vertical jump at the 50 percent level. 2. The second male graders, coming up with equalized values such as 232㎝ in a standing board jump, 33.5m in five seconds' running and 42m in throwing, and 57㎝ in a vertical jump at the 50 percent level. 3. The third male grades, coming up with equalized values such as 242㎝ in a standing board jump, 34.8m in five seconds' running and 45m in throwing, and 57㎝ in a vertical jump at the 50 percent level. 4. The first female graders, coming up with equalized values such as 168㎝ in a standing board jump, 25.8m in five seconds' running and 10m in throwing, and 34㎝ in a vertical jump at the 50 percent level. 5. The second female grader, coming up with equalized values such as 174㎝ in a standing board jump, 25.8m in five seconds' running and 17m in throwing, and 31㎝ in a vertical jump at the 50 percent level. 6. The third male graders, coming up with equalized values such as 187㎝ in a standing board jump, 27m in five seconds' running and 16.6m in throwing, and 30㎝ in a vertical jump at the 50 percent level.

수출용 축양품종 가지의 염절임 공정의 최적화

남학식,김남우,황성희,윤광섭,신승렬 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

가지의 수출 경쟁력 향상을 위한 한 방안으로 수출용 가지품종인 축양품종을 염가공품으로 개발하기 위하여 염절임공정을 최적화하였다. 염절임 시간, 절임온도, 염농도를 독립변수로 하고 절임 후 수분함량, 염도, 표면과 내부의 색도 등을 종속변수로 하여, 중심합성계획법으로 실험을 설계하여 최적조건을 얻고자 하였다. 수립된 이차회귀모형으로 예측식을 수립할 수 있었으며 특히 수분함량이나 염도, 표면과 내부의 L과 b 값에 대하여 높은 적합도를 보여 최적조건을 수립하기 위한 제한 변수로 선정하였다. 염농도를 중심점(30%)에서 고정시켜서 얻은 절임시간과 온도는 각각 5.5~6.5일과 13-17℃였으며, 이때의 종속변수의 제한 요건으로는 수분함량 84%이하, 염도 14%이하 표면 L값 10-20, b값 0이하, 내부 L 값은 70-75, b 값이 16-18이었다. This study was conducted to the optimize salting process of eggplant for development new product and enhancement quality for export. Three variables by five level central composite design and response surface methodology were used to determine optimum conditions for salting time, temperature and salt concentration. Optimization of the process was conducted using the combination of the moisture content, salinity and color of surface and inside of salted eggplant. The regression polynomial model was suitable (P>0.05) by Lack-of-Fit analysis with highly significant. To optimize the process, based on surface response and contour plots, the individual contour plots of the response variables were superimposed. The optimum conditions for this process were 6 days and 15℃ at 30% concentration under the optimum of restricted variables as moisture content was below 84%, salinity was below 14%, L and b value of surface were 10 to 20 and below 0, L value and b value of inside were 70 to 75 and 16 to 18.

시간-주파수 해석법에 의한 CFRP의 파괴 거동

남기우,안석환,이상기,김현수,문창권 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.1

신호처리법으로 현재 많이 사용되고 있는 푸리에 변환은 신호의 주파수 성분이 사간에 따라 어떻게 변화하는지를 표현하지 못한다. 따라서 최근 이와 같은 푸리에 변환의 단점을 보완하여, 신호의 시간과 주파수에 대한 정보를 동시에 표현할 수 있는 시간-주파수 해석법들이 개발되었다. 본 연구에서는 음향방출을 이용하여 복합재료의 주요 발생원으로 알려져 있는 기지균열, 섬유분리, 섬유파괴 및 층간분리 등과 같은 파괴기구를 해석하였다. 각각의 파괴특성이 나타나도록 시험편을 제작하여 인장시험 시 검출된 음향방출신호의 시간-주파수 해석을 통해 전체 파괴기구의 특징을 분석하였다. Fourier transform has been one of the most common tools to study the frequency characteristics of signals. With the Fourier transform alone, however, it is difficult to tell whether signal's frequency contents evolve in time or not. Except for a few special cases, the frequency contents of most signals encountered in the real world change with time. Time-frequency analysis methods are developed recently to overcome the drawbacks of Fourier transform, which can represent the information of signals in time and frequency at the same time. In this study, damage process of cross-ply carbon fiber reinforced plastic (CFRP) under monotonic tensile loading was characterized by acoustic emission. Different kinds of CFRP specimens were used to determine the characteristics of AE signals. Time-frequency analysis methods were employed for the analysis of fracture mechanisms in CFRP such as matrix cracking, debonding, fiber fracture and delamination.

하수슬러지 중 노말핵산추출물질(HEM)함량 분석 및 토양 사용시 연간 오염부하량 추정

남재작,박우균,임동규,이상학 한국환경농학회 2002 한국환경농학회지 Vol.21 No.1

하수슬러지 중에 존재하는 HEM에 대해 분석하고, 이를 토양에 사용할 때 발생하는 연간 오염부하량에 대해 추정하였다. 전국의 하수처리장에서부터 수집된 하수슬러지 시료 84점에 대해 분석한 HEM의 평균함량은 27.7±26.5 g/㎏이었고, 최소 1.05에서 최대 194 g/㎏을 나타내어 분석시료간에 큰 편차를 보였다. 하수 처리장이 위치한 도시의 규모에 따라 5단계로 나누었을 매 HEM의 함량은 광역시, 대도시, 중도시, 소도시, 농촌지역에 각각 22.7±16.7, 33.3±25.8, 22.0±8.7, 31.0±30.8, 27.7±25.1 g/㎏을 나타내었다. 미국 EPA의 하수슬러지 토양시용량 기준을 적용하여 농경지, 산림, 공공용지, 개량용지로 분류하고 각각 7,000, 26,000, 18,000, 74,000 ㎏/㏊ (건물량 기준)를 하수슬러지 최대 사용량으로 가정하여 HIM의 연간 오염부하량울 계산하였다. 건물량 기준으로 시용량이 7,000 ㎏/㏊인 농경지의 경우 최대 1,032 ㎏/㏊의 HEM 부하량을 나타내었고, 개량용지 시용기준인 74,000 ㎏/㏊를 적용한 경우 HEM의 연간 오염부하량은 10,908 ㎏/㏊으로 추정되었다. 그 외 산림기준을 적용할 때는 최대 3,832㎏/㏊, 공공 용지 기준을 적용할 때는 최대 2,653 ㎏/㏊의 연간 오염부하량이 추정되었다. To estimate annual pollutant loading rate of n-hexane extractable material (HEM) to soil 84 sewage sludge samples were analyzed. The average content of HEM was 27.7±26.5 g/kg, and ranged from 1.05 to maximum 194 g/kg. According to the number of population of the city the areas were classified as five regions, i.e. big, large, middle, small, and rural area in which the waste water treated plants were grown. The contents of HEM were 22.7±16.7, 33.3±25.8, 22.0±8.7, 31.0±38.8, 27.7±25.1 g/kg, in big, large, middle, small, and rural area, respectively. The applicated areas with sewage sludge to land also were divided as 4 sites by US EPA criteria such as agricultural, forest, public contact sites, and reclamation site. The quantitation of annual pollutant loading rate was estimated using a function (99 percentile concentration = X￣+ 30) when the application amounts were in dosages of 7,000, 26,000, 18,000, 74,000 kg/ha in 5 regions, respectively. The annual pollutant loading rate of HEM to soil by sewage sludge application was maximum 1,032, 3,832, 2,653, and 10,908 kg/ha in agricultural, forest, public contact sites, and reclamation site, respectively.

저강도 자전거에르고미터 운동프로그램이 혈장지질 농도에 미치는 영향

성봉주,남형천,김상우 한국학교체육학회 2001 한국학교 체육학회지 Vol.11 No.1

본 연구의 목적은 8주간의 저강도 자전거 에르고미터 운동프로그램이 혈중지질(TC, LDL-C, HDL-C, TG, TC/ HDL-D에 미치는 영향을 규명하는데 있다. 연구의 대상자는 서울시 K대 여자학생 중 각종 질병이 없고, 건강 상태가 양호한 16명을 무작위 표집하고, 두 집단으로 구분하였다 · 실험집단(8명), 통제집단(8명). 실험전 실험집단의 최대심박수를 측정하기 위하여 Bruce Protocol로 운동부하 검사를 실시하였다. 혈액검사는 훈련 전과 훈련 4주 후, 훈련 8주 후에 전완 정맥에서 채혈하였다. 실험집단의 운동프로그램은 (1) 운동강도와 기간은 각자의 60%HRmax인 저강도로 주 3회, (2) 운동시간은 20분부터 시작하고 2주마다 10분씩 증가, (3) 운동기간은 8주. 자료 분석은 두 집단과 처치기간에 따른 실험변인(TC, LDL-C, HDL-C, TG, C-Ratio)에 미치는 반응을 검증하기 위해 2(실험 집단, 통제 집단)×3(훈련 전, 훈련 4주 후, 훈련 8주 후) 반복적 측정의 이원 변량분석 및 두 집단간의 독립적 그룹의 T-test, 각 집단의 처치기간별 반복적 측정의 일원 변량분석을 실시하였다 유의수준은 5%로 하였다. 이러한 절차로부터 다음과 같은 결론을 얻을 수 있었다. 첫째, 두 집단간 처치기간별 TC수준의 차이는 유의하지 않았다. 또한 각 집단의 처치기간별 TC수준에서 실험집단은 유의한 감소를 보이지는 못했다. 통제집단은 유의한 차이가 나타나지 않았다. 둘째, 두 집단간 처치기간별 LDL-C수준의 차이는 유의하지 않았다. 또한 각 집단의 처치기간별 LDL-C 수준에서 실험집단은 훈련 전과 훈련 4주 후에 유의하게 감소하였다. 통제집단은 유의한 차이가 나타나지 않았다. 셋째, 두 집단간 처치기간별 HDL-C수준의 차이는 훈련 전에는 유의하지 않았으나 훈련 4주 후와 훈련 8주 후에서는 유의하게 나타났다. 또한 실험집단의 처치기간별 HDL-C수준에서 유의한 증가를 보이지는 못했다. 통제집단은 유의한 차이가 나타나지 않았다. 넷째, 두 집단간 처치기간별 TG수준의 차이는 유의하게 나타나지 않았다. 또한 실험집단의 처치기간별 TG수준에서 유의한 감소를 보이지는 못했다. 통제집단은 유의한 차이가 나타나지 않았다. 다섯째, 두 집단간 처치기간별 C-Ratio수준의 차이는 유의하지 않았다. 또한 실험집단의 처치기간별 C-Ratio수준에서 유의한 감소를 보이지는 못했다. 통제집단은 유의한 차이가 나타나지 않다. 이상과 같은 결과를 종합해보면 저강도 자전거에르고미터 운동프로그램도 일반 여자대학생들의 혈장지질농도에 긍정적인 영향을 미쳤다. 그러나 콜레스테롤 대사의 전반적 향상을 위해서는 자전거에르고미터의 운동강도를 높이거나 운동기간을 늘여야 할 것으로 사료된다. The purpose of this study is to investigate the effect of low intensity bicycle ergometer exercise program on blood lipid. The subject of this study, 16 fㄷmale university students in Seoul who are health, and have no diseases, are randomly sampled and divided into two groups : experimental group(n=8), and control group (n=8). Pre-test is done the exercise load to measure for HRmax of experimental group using Bruce protocol. I gather blood from antecubital vein of experimental group for blood test at three times, just before test, after 4 week exercise and 8 week exercise. The experimental group takes bicycle ergometer training on these conditions: (1) exercise three times in a week at low intensity of 60^ HRmax, (2) Increase time in 10 Min. at every two weeks, (3) group was trained for 8 weeks. I use statistical techniques for data analysis, 2*3 repeated measure two way ANOVA to determine the differences between tow groups and training times, independent group T-test to determine the differences between two groups, and repeated measure one way ANOVA for training time. The 5% level of significance is utilized. The following results are obtained this study; First, the TC levels of two groups are not significantly different in the training period. Although TC level of experimental goop decrease in after-exercise (4 week, 8 week) to comparison with TC level of before-exercise, it is not significant decrease. And it was no significant difference in control group. Second, the LDL-C levels of two groups are not significantly different in the training period. The LDL-C level of experimental group decreased in after-exercise (4 week, 8 week) in comparison with before-exercise. Specially it decrease significant after 4 week exercise. And it is no significant difference in control group. Third, the HDL-C levels of two groups are not signif4cantly different before exercise, but are significant different after exercise (4 week, 8 week). Although the HDL-C level of the experimental group decrease in after-exercise (4 week, 8 week), but it is not significant decrease. And it is not significant difference in control group. Fourth, the TG levels of tow groups are not significantly different during training period. Although the TG level of experimental group decrease in after-exercise(4 week, 8 week) in comparison with before-exercise, but it is not significant decrease. And it is no significant difference in control group. Fifth, the C-Ratio levels of tow groups are not significantly different during training period. Although the C-Ratio level of experimental group decrease in after-exercise(4 week, 8 week) in comparison with before- exercise, but it is not significant decrease. And it is no significant difference in control group. From the result, because the low intensity bicycle ergometer exercise program effect blood lipids of the female university students, it will contribute for improving health and the prevention adult disease. Synthesizing all the results, low intensity bicycle ergometer exercise program had a positive effect on LDL-C, HDL-C. From the results, Furthermore, In order to maximize the effect of training. it will be better to increase the period of exercise.

貨煥信用狀統一規則의 適用上의 諸問題點 : UCP 500의 第24條(Sea Waybill)를 中心으로

南豊祐,韓祥鉉 강남대학교 1999 (강남대학교)논문집 Vol.34 No.3

Rohrer지수에 의한 한국과 중국 연변지역 조선족 아동의 체격비교

남은우,박기만,배성권 韓國學校保健學會 1996 韓國學校保健學會誌 Vol.9 No.1

This paper was performed to comparative study with the children's body strength status of Ethinc Korean in China and Korea. The subjects of this study were the students of each elementary school located in a rural and a urban areas in China. To compared the elvel of their body strength status, we investigated to the students of 2 elementary schools as comparative group on Kojae Area in Korea. We used student's physical record in the school. The contents of the study were the body weight, the chest circumstance, and the body height of the subjects. To make out the level of the students' physique, we measured by Rohrer Index output through above body height and body weight of them. The results of this were as follows; 1. In the case of Ethinc Korean in China, the average body weight(kilogram) is 28.94±6.68 in male 28.51±7.58 in famels. Comparatively, the average body weight in the case Korean is 31.99±7.23 in male and 31.74 ±7.82 in female. The average body height of Ethnic Korean is inferior about 2~5 Kilograms to that of Korean. 2. The average chest circumstance(centimeter) is 63.51±5.21 in male 63.05±5.87 in female of Ethinc Korean, 65.54±6.43 in male and 64.57±7.81 in female of Korean. Compared to between two group, the average chest circumstance of Ethinc Korean is inferior about 2~4 centimeters to that of Korean. 3. The average body height(centimeter) of Ethinc Korean is 135.28±8.96 in male 134.82±9.74 in female, and that of Korean is 136.68±9.12 in male and 136.10±9.92 in female. Like the preceding below results, the average body height of Ethinc Korean is inferior about 2~5 centimeters to that of Korean. 4. Compared to the fullness degree of physique between two group using Rohrer Index, Ethinc Korean is thiner than Korean. 5. Temporary, in case of children from 10 to 12 years old, female is superior to male through the index contained of the body weight, the chest circumstance, and the body height. It seems that female and male had different maximum growth age.