濟州道 改良草地에 있어서 띠(Imperata cylindrica)의 季節的 年次的 變化

趙南棋,金翰琳,宋昌吉 제주대학교 1983 논문집 Vol.16 No.-

本 硏究는 濟州道 中山間地帶의 自然草地(Imperata cylindrica/Zoysia japonica type)를 開墾한 後 1 ha當 Dactylis glomerata 17kg, Festuca arundinacea 7kg, Lolium multiflorum 2kg, Trifolium repens 2kg의 導入牧草를 混播(28kg)하여 家畜放牧에 利用되고 있는(1977∼1982年) 改良草地를 對象으로하여, 띠(Imperata cylindrica)植生의 季節的ㆍ年次的變化過程을 調査한 結果를 要約하면 다음과 같다. 1. 每年 季節에 따르는 草長ㆍ收量ㆍ密度ㆍ被度 等 植生의 季節的 變化에 있어서는 秋期에 가장 優勢하였고, 春期에는 低調하게 나타났다. 2. 改良後 年度別 草長의 變化는 1年草地에서 34.26cm이었으나 점차 增加되어 6年草地에서는 49.35cm이었는데, 이 變化狀態의 回歸方程式은 y=-0.13x²+4.41x+28.50이었다. 3. 年數의 經過에 따르는 密度의 變化는 1年草地에서 0.76%였던 것이 年數가 지남에 따라 급격히 增加되어 6年草地에 이르러서는 32.97%로 나타나고 있는데, 이 變化를 y=0.10x²+0.32x-2.30의 回歸方程式으로 表示할 수 있었다. 4. 草地造成 以後 年次別 被度의 變化는 1年草地에서 1.05%, 2年 1.38%, 3年 2.46%, 4年 17.11%, 5年 27.56%, 6年草地에서 36.66%로 每年 增加(y=1.40x²-2.36x+1.39)되었다. 5. 收量의 年次的 變化는 1年草地에서 1.95kg이었으나 年數가 지남에 따라 점차 增加되어 6年草地에서는 1,107.35kg으로 나타났는데, 이 變化狀態를 y=29.11x²+53.52x-154.67로 表示할 수 있었다. This study was conducted to examine the yearly and seasonal changes of Imperata cylindrica on the improved pasture of the mountain districts around Mt. Halla in Cheju Island. For this study, the researcher had investigated for six years from 1977 to 1982 the improved pasture which had been used for grazing from 1977 to 1982. The pasture was reclaimed from native grassland. The mixed seeds of 17kgs' Datylis glomerata, 7kgs' Festuca arundinacea, 2 kgs' Lolium multiflorum and 2kgs' Trifolium repens were sowed per 1 ha. The results of this study are the following : 1. The seasonal changes of Imperata cylindrica : its length, weight, density and coverage are heighest in Autumn, and are lowest in Spring every year. 2. The yearly change in the length of Imperata cylindrica : its length is gradually increased from 34.26 cm in the first year after the reclaimation to 49.35 cm in the 6th year. This change can be represented by the regression equation, Y=-0.13x²+4.41x+28.50. 3. The yearly change in its density : its density is rapidly increased from 0.73% in the first year to 32.97% in the sixth year. The results of this change can be obtained with the regression equation, Y=0.10x²+0.32x-2.30. 4. The yearly change in its coverage : its coverage is increased year by year (1.05% in the 1st year, 1.38% in the 2nd year, 2.46% in the 3rd year, 27.56% in the 5th year, and 36.66% in the 6th year). This change can be calculated with regression equation, Y=1.40x²-2.36x+1.39. 5. The yearly change in its weight : the weight is also increased year after year from 1.95 kg in the first year after reclaimation to 1107.35kg in the sixth year. This change can be calculated with the regression equation of y=29.11x²+53.52x-154.67.

인덕터의 DC 바이어스 특성을 고려한 대용량 DC-DC 컨버터의 손실계산

조영창(Young-Chang Jo),최주엽(Ju-Yeop Choi),정승기(Seung-Ki Jung),최익(Ick Choy),송승호(Seung-Ho Song) 대한전기학회 2011 전기학회논문지 Vol.60 No.4

It is necessary to accurately predict converter losses for optimized design of a high-power DC-DC converter. The losses of switching devices and inductor among the elements of the converter take significantly greater proportion. The current ripple will be determined by the size of the inductance and this inductance value varies depending on the DC amount of inductor current. As the inductance changes according to load current, the change influences not only the inductor loss itself but also the total converter loss. In this paper, for more accurate design of a bi-directional DC-DC converter for 30㎾-class energy storage system, more accurate computational model is proposed considering inductance variation according to the load current change. The inductance changes using variable magnetic cores are verified and converter efficiency is tested through simulations and experiments.

한국인 A 형 간염 환자에서 A 형 간염 바이러스의 유전자형에 관한 연구

권오상,송기준,박상훈,송진원,김재선,김종헌,변관수,이창홍,연종은,백락주,박영태 대한간학회 2000 Clinical and Molecular Hepatology(대한간학회지) Vol.6 No.3

$quot;목적: 한국에서 과거 A형 간염은 낙후된 개인위생 및 자연환경으로 유소아기에 불현성 감염으로 앓고 지나가게 되어 청소년 및 성인에서 HAV에 대한 항체 보유율이 매우 높고 성인에서의 급성 A형 간염은 매우 드물게 보고되었다. 그러나 고도의 경제성장과 더불어 식생활, 위생상태의 개선, 상하수도의 보급 등으로 유소아기의 A형 간염 이환율이 현격히 감소하면서 청소년 및 성인에서 HAV 항체가 없는 인구집단 즉 HAV에 감염되면 현증 간염이 발생할 수 있는 인구 집단이 증가되어 최근 수 년 사이에 현증 A형 간염이 폭발적으로 증가하게 되었다. 이에 저자는 HAV의 유전자형을 알아보고 이미 보고된 각국의 HAV와 연관성을 알아보고자 본 연구를 시행하였다. 대상과 방법: 1994년부터 1998년까지 한국에서 산발적으로 발생한 18예의 A형 간염 환자의 혈청에서 HAV RNA를 추출, 역전사-중화효소 연쇄반응법(RT-PCR)으로 증폭하여 직접 염기서열의 분석을 통해 국내 HAV 사이에 염기서열, 아미노산의 차이 등을 알아보고 세계 각국에서 보고되었던 HAV 분리주들과 비교하였다. 결과: HAV 게놈의 특정부위인 VP1/2A 연결부위 168 bp 길이에서 시행한 염기서열 분석 결과 한국에서 분리된 18예의 HA이러스는 최근 해외에서 유입된 바이러스일 가능성은 적으며 따라서 장기간 국내에서 토착화된 바이러스일 것으로 생각된다. $quot;Background/Aims: Studies of genotypes and changes in nucleotide and amino acid sequences of hepatitis A virus (HAV) may provide valuable information on the epidemiological aspects of a particular region. In Korea the prevalence of anti-HAV in the 1 - 20 year age group declined from 60% in 1980 to 9% in 1995. As a result this age group has a high risk of HAV infection. Actually over 1,500 cases of clinically overt hepatitis A occurred in 1998 while few cases of clinical hepatitis A had been reported until the early 1990s. The aims of this study are to determine the genotypes of HAV which have been circulating in Korea and to define the phylogenetic relationships of geographically defined isolates. Method: From 1994 - 1998 a total of 18 serum specimens was obtained from patients in Korea with sporadic form acute hepatitis A. The HAV nucleic acid from serum specimens was subjected to genomic sequence analysis following viral RNA extraction, reverse transcription and amplification of the cDNA by PCR. DNA sequencing was performed in both directions of each PCR product. Results: All isolates clustered within the subgenotype IA irrespective of the geographic locations and timing of the clinical hepatitis. Among 18 Korean isolates, 9 isolates had 2 amino acid sequence changes and 2 isolates had 1 amino acid sequence change. These changes in the amino acid sequences are unique and have never been reported in HAV subgenotype IA. Conclusions: All isolated HAV had genotype (IA). Eleven of 18 isolates had unique changes in amino acid sequences. These data indicate that the endemic HAV has been circulating in Korea over a long period of time.

濟州島 人工草地 植生의 經時的 變化에 관한 硏究 : 草種의 李節 및 年次的 變化 The Seasonal and Annual Changes in Species

金翰琳,趙南棋,宋昌吉 제주대학교 1981 논문집 Vol.13 No.-

本 硏究는 濟州道 改良牧草地에 있어서 草種組成의 季節 및 年次的 變動狀態를 調査한 結果이며 그 槪要는 다음과 같다. 1. 改良年度別 草種의 變動狀態는 1975年 草地에서 37種이었으나 年數가 經過함에 따라 種數가 增加되어 1980年 草地에서는 130種이었으며 每年 季節에 따르는 種의 數는 夏期에 가장 많았고 春期에는 種의 分布가 가장 적었다. 2. 改良年度別 生育型의 分布變動狀態는 1975年 草地에서 一年生 12種, 多年生 25種, 木本 1種이었으나,年數가 지남에 따라 每年 增加되어 1980年 草地에서는 一年生 42種, 多年生 88種, 木本 5種이었으며, 每年 季節에 따르는 生育型에 있어서는 一年生은 秋期에서, 多年生은 夏期에서 가장 많은 種이 分布되었고 木本雜草는 季節에 變化가 없었다. This study was conducted to investigate the seasonal and annual changes in species on the impoved pastures in Cheju Island. The results are as follows : 1. The annual changes in the number of intruding weed species were obsered. 33 weed species were found in 1975 and increased year after year to 127 species in 1980. The seasonal changes of species by year were also observed. In summer more species ranged than in any other seasons and the number of species growing in spring was found fewer than in other seasons. 2. The distributional changes in annual and perenial plant were brough on. 10 species of annual plant, 23 species of perennial plant, and one species of arbor were found in 1975 and increased respectively year by year to 44 species (annual plant), 86 species(perennial plant) and 5 species (arbor) in 1980. The seasonal changes in ecology by year showed a tendency that more species of annual plant ranged in autumn, and more perennial plants in summer than in any other season. But there is no change in the species of arbor.

만성 화농성 중이염의 세균학적 고찰

조남순,이영훈,강기훈,최제환,송태현,이병돈,장혁순,강주원,김연준 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background and Objectives : Chronic suppurative otitis media is one of the frequent diseases in otolaryngologic field. It is important to choose of antibiotics in the management of infectious disease. But the organisms in infections have been changed and resistance to antibiotics has been grown as the development of antibiotics has been achieved. And so it has been necessary to recognize the changes of organisms and resistance in antibiotics. Our study was performed to identify the pathogens isolated from discharges in chronic otitis media and evaluate the antibiotic agents, to recognize the change of to resistance to·antibiotic agents and to use the appropriate anitiotics. Materials and Methods : Retrospectively, authors reviewed the backeriologic study of 114 cases of chronic suppurative otitis media who visited the Department of Otolaryngology, Soonchunhyang University in Seoul from Nov. 1996 to Oct. 1999 and analyzed 101 strains and its sensitivity test to various antibiotics. Result : In 93 cases in which pathogenic organism was isolated, single infection was 85 cases(91.4%) and mixed infection was 8 cases(8.6%). The most frequent pathogenic organism was Staphylococcus aureus(59.4%), Pseudomonas aeruginosa(13.9%), Providencia(4.8%), Alcaligenes(3.0%) and Streptococcus pneumoniae(2.9%) were the next. Methicilline-Resistance Staphylococcus aureus was 34 cases(57.6%) of Staphylococcus aureus. Staphylococcus aureus was sensitive to Vancomycin(96.7%), Trimethoprim-Sulfamethoxazole (57.6%), Cefuroxime(42.4%) and Ciprofloxacin(40.7%) but resistant to Penicillin(94.9%). Pseudomonas aeruginosa was sentitive Amikacin(100%), Ciprofloxacin(100%) and Gentamicin(67%). Conclusion : As MRSA recently was wide spread in community accquired infection as well as nosocomial infection, we must consider strict control of MRSA.

Diagnostic Significance of Brainstem Auditory Evoked Potentials in Microvascular Decompression of Patients with Hemifacial Spasm or Trigeminal Neuralgia

Park, Sang-Koo,Lim, Sung-Hyuk,Park, Chan-Woo,Park, Jin-Woo,Chang, Sung-Ho,Park, Keun-Hye,Park, Hae-Ja,Song, Ji-Hye,Uhm, Dong-Ok,Kim, Ki-Bong 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.1

The purpose of this study was to analyse brainstem auditory evoked potentials (BAEP) wave change data during microvascular decompression (MVD). The nerve function of Cranial Nerve VIII is at risk during MVD. Intraoperative monitoring of BAEP can be a useful tool to decrease the danger of hearing loss. Between January and December 2009, 242 patients had MVD for hemifacial spasm (HFS) and trigeminal neuralgia (TN). Among intraoperative BAEP changes, amplitude of V-V' was the most frequently observed during cerebellar retraction and decompression step of the MVD procedure. 138 patients (57%) had no BAEP change while 104 patients (42.98%) had BAEP change. 69 patients (28.5%) had Type A-I, 16 patients (6.6%) had Type A-II, 5 patients (2.1%) had Type B, and 13 patients (5.37%) had Type C. MVD is a surgical procedure to relieve the symptoms (e.g. pain, muscle twitching) caused by compression of a nerve by an artery or vein. During BAEP intraoperative monitoring, the surgical step is important in interpreting the changes of wave V. Several potential mechanisms of injury may affect the cochlear nerve, and complete loss of BAEP is often associated with postoperative hearing loss. Intraoperative BAEP monitoring may provide an early warning of hearing disturbance after MVD.

Diagnostic Significance of Brainstem Auditory Evoked Potentials in Microvascular Decompression of Patients with Hemifacial Spasm or Trigeminal Neuralgia

( Sang-koo Park ),( Sung-hyuk Lim ),( Chan-woo Park ),( Jin-woo Park ),( Sung-ho Chang ),( Keun-hye Park ),( Hae-ja Park ),( Ji-hye Song ),( Dong-ok Uhm ),( Ki-bong Kim ) 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.1

The purpose of this study was to analyse brainstem auditory evoked potentials (BAEP) wave change data during microvascular decompression (MVD). The nerve function of Cranial Nerve VIII is at risk during MVD. Intraoperative monitoring of BAEP can be a useful tool to decrease the danger of hearing loss. Between January and December 2009, 242 patients had MVD for hemifacial spasm (HFS) and trigeminal neuralgia (TN). Among intraoperative BAEP changes, amplitude of V-V’ was the most frequently observed during cerebellar retraction and decompression step of the MVD procedure. 138 patients (57%) had no BAEP change while 104 patients (42.98%) had BAEP change. 69 patients (28.5%) had Type A-I, 16 patients (6.6%) had Type A-II, 5 patients (2.1%) had Type B, and 13 patients (5.37%) had Type C. MVD is a surgical procedure to relieve the symptoms (e.g. pain, muscle twitching) caused by compression of a nerve by an artery or vein. During BAEP intraoperative monitoring, the surgical step is important in interpreting the changes of wave V. Several potential mechanisms of injury may affect the cochlear nerve, and complete loss of BAEP is often associated with postoperative hearing loss. Intraoperative BAEP monitoring may provide an early warning of hearing disturbance after MVD.

상피성 암세포주에 대한 녹차 Catechin의 효과

박정현(Jeong Hyun Park),김대중(Dae Joong Kim),한장희(Jang Hee Hahn),김홍태(Hong Tae Kim),정용욱(Yong Wook Jung),성훈기(Hoon Ki Sung),김주영(Joo Young Kim),송인환(In Hwan Song),성언기(Eon Gi Sung),이융창(Yung Chang Lee) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.5

Catechin은 녹차에서 추출되는 폴리페놀의 주요성분으로 고혈압 및 동맥경화의 예방효과, 당뇨억제효과, 항산화작용, 항암작용 등에 직접 관여하는 물질이다. 본 연구에서는 태평양에서 제공받은 녹차 catechin을 이용하여 상피성 암세포주인 A549 (폐암)와 EATC (복수암)세포에 투여한 후 처리농도와 시간에 따른 변화를 비교, 관찰함으로써 녹차의 효과와 작용기전을 밝혀내는데 목적이 있었다. 본실험은 A549 세포와 EATC 세포를 배양한 후 녹차 catechin을 1, 10, 100, 500 μg/ml의 농도로 48시간 동안 처리하였고, 광학현미경, 공초점현미경, 전자현미경 등을 이용하여 세포의 구조적 변화를 확인하였으며 MTT분석, 전기영동, 유세포분석기 등을 사용하여 세포 손상정도를 파악하였다. A549 세포에서는 catechin 1 μg/ml와 10 μg/ml 농도에서는 대조군에 비하여 큰 변화를 관찰할 수 없었다. 100 μg/ml catechin을 처리하였을 때 세포내의 검은 과립들의 수가 증가하였고 층판소체의 손상이 나타났다. 세포주기의 장애가 나타나 DNA 합성전기에 있는 세포들의 수가 급격히 증가하였다. 500 μg/ml 농도에서는 층판소체와 사립체의 파괴가 심하게 나타났으며 세포생존율이 감소하였고 세포주기의 장애도 관찰되었다. EATC 세포에서는 catechin의 농도가 A549 세포의 경우보다 낮은 농도에서도 세포증식 억제 및 세포손상 효과가 나타났다. 10 μg/ml 농도에서도 세포의 위축과 생존율의 감소가 일어났으며 전기영동상에 괴사되는 세포들이 파악되었다. 100μg/ml catechin을 처리하였을 때 자연사의 형태학적 관찰, 전기영동, 유세포분석 등에서 자연사 과정에 있는 세포들이 많이 나타났다. 결과적으로 녹차 catechin을 배양한 상피성 암세포에 투여함으로서 세포의 생존율과 증식이 억제되었고 그 과정에서 괴사, 자연사, 세포주기의 장애 등이 관여하는 것으로 생각된다. 그러나 일련의 세포손상과정을 유도하는 데 있어 세포의 종류, 처리 시간, 농도에 따라 다소 차이가 있음을 확인하였다. Catechin is main component of polyphenol extracts from green tea, it is associated with prevention of hypertension and atherosclerosis, anti-diabetic effect, antioxidant, antitumor. The purpose of this research is to investigate the effect and its mechanism of green tea catechin on epithelial cancer cell lines in various concentrations and durations. For this study, epithelial cancer cell lines, A549 (lung cancer), EATC (Ehrlich-Lettre ascites tumor cell) were used. Inverted, light, confocal and electron microscopes were applied to find morphological changes. MTT assay, flowcytometric analysis, gel electrophoresis were used to compare severity of cellular damages to control after exposure to 1, 10, 100 and 500 μg/ml catechin for 48 hours. In the A549 cells, after 1 μg/ml and 10 μg/ml catechin treatments, there was no notable changes. However, exposure to 100 μg/ml catechin induced increase of cytoplasmic granules, destruction of lamellar body, inhibition of cell cycle, especially G0/G1. In the early phase of 500 μg/ml catechin administration, decrease of cell population, severe destruction of lamellar bodies and mitochondria, derangement of cell cycle were shown. In the EATC, such as those effects occurred after exposure to lower concentration of catechin than in that of A549 cells. After exposure of 10 μg/ml catechin, rounded-up cells and necrotic cells were found. Whereas, most of cells were under apoptotic changes-cytoplasmic condensation, nuclear fragmentation, cellular shrinkage, ladder pattern in the electrophoresis, when administrated 100 μg/ml catechin. These results suggested that exposure of catechin induced severe cellular damage and growth inhibition in dose- and time-dependent manner. And we confirmed that these effects of catechin were involved with apoptosis, necrosis and cell cycle arrest and were quite different according to cancer type. Therefore, much more research would be demanded before clinical application of catechin to human cancer therapy and this study would be the basic source for further study of green tea.

播種量에 따른 靑刈油菜의 生育反應 및 生草 收量變化

宋昌吉,趙南棋 濟州大學校 亞熱帶農業硏究所 1995 亞熱帶農業硏究 Vol.12 No.-

本 硏究는 濟州道 火山灰土壤에서 播種量 差異에 따른 靑刈油菜의 生育反應 및 靑刈收量에 미치는 影響을 究明하기 위하여 遂行하였으며, 그 結果를 要約하면 다음과 같다. 1. 草長은 900g과 800g, 播種區에서 가장 길었으며, 1000g, 700g, 600g, 500g 順位로 짧았다. 2. 葉數, 葉重, 莖重, 莖直徑과 分枝數등은 播種量이 많을수록 작았으며, 葉幅과 葉長은 播種量이 많아질수록 넓고 커졌다. 3. 根長은 播種量이 많아질수록 작아졌으며, 根重은 800g과 900g 播種區에서 무거웠고 500g 播種區에서 가장 작았다. 4. 靑刈收量은 900g, 800g 및 1000g 播種區에서 가장 많고, 이들간에는 有意差가 없었으나, 500g과 600g 播種區에서 靑刈收量은 매우 적었다. 5. 草長은 靑刈收量, 葉長과는 高度의 正의 相關關係를 보였으며, 葉幅과 根重과는 正의 相關을 莖重과 分枝數와는 負의 相關關係를 보였다. The study is accomplished to investigate the growing response of soiling rape by the difference of seeding rate on the soil of volcanic ashes in Cheju-Do. The results of this study are as follows; 1. The plant height on the seeding plot of 900g, 800g revealed longest, but short on seeding plot of 1000g, 700g, 600g and 500g in that order. 2. The more seeding rate was, the smaller revealed the number of leaf, leaf weight, stem weight, stem diameter and number of branch. The more seeding rate, the wider and the larger the leaf width and the leaf length became. 3. The more seeding rate was, the shorter the root length became. Root weight was heavier on the seeding plot of 800g and 900g, but smallest on the seeding plot of 500g. 4. The harvesting quantity of soiling rape revealed largest on the seeding plot of 500g, 600g, and 1000g. There were no significant difference among them, but the harvesting quantity revealed very small on the seeding plot of 500g and 600g. 5. There was a lot positive correlation between the plant height and the harvesting quantity of soiling rape, but positive correlation between leaf width and he root weight, negative correlation between the stem weight and he number of branch.

ALUMINUM HYDROXIDE SOL에 첨가된 SALT에 의한 유변학적 특성 변화

송기창 建陽大學校 1994 建陽論叢 Vol.- No.3

Aluminum hydroxide sols prepared from the hydrolysis of aluminum alkoxide consist of boehmite particles stabilized electrostatically in water. But they are destabilized and flocculated when salts (e.g., NaCl, Na₂SO₄) are added to the sols. This result appears as a change in the rheological behavior of the sols from Newtonian to Shear thinning.