심비디움 모자이크 바이러스의 핵산과 외피단백질에 관한 연구

배소현,장무웅 嶺南大學校 基礎科學硏究所 1994 基礎科學硏究 Vol.14 No.-

심비디움의 잎에 괴저 줄무늬 증상주에서 분리한 심비디움 모자이크 바이러스(CyMV-K)를 카틀레야와 팔레높시스에 증식시켜 순화 시료로 하였다. 순화된 CyMV-K는 길이 480-500nm, 폭 13nm의 약간 완곡형의 사상입자였다. 순화된 CyMV-K 시료의 자외선 흡광도를 조사한 결과, 260nm에서 최대, 248nm에서 최소값을 나타내는 전형적인 핵단백질의 자외선 흡수곡선을 보였다. Phenol/chloroform 추출에 의해 정제된 CyMV-K RNA의 분자량은 6.45Kb로 측정되었다. CyMV-K coat protein의 분자량 측정을 위해 SDS-PAGE법으로 전기영동한 결과, 분자량은 약 2.51 x 10⁴ 달톤으로 확인되었다. CyMV-K, CyMV-A, CyMV-J를 한천겔 이중확산법과 면역전자현미경법으로 혈청학적 관계를 조사한 결과, 혈청형이 거의 동일하였다. Cymbidium mosaic virus(CyMV-K) from Cymbidium sp, showing necrotic stripe symptom on the leaves was multiplied in Cattleya sp, and Phalaenopsis sp, and then purified. The purified CyMV-K was slightly flexible particles which have lengths in the range of 480-500 nm and widths of 13 nm. Ultraviolet absorption pattern of purified CyMV-K indicated the maximum at 260 nm and minimum at 248 nm, which was typical pattern of viruses. The molecular weight of CyMV-K RNA prepared by the extraction of phenol/chloroform was about 6.45 Kb. The molecular weight of CyMV-K coat protein estimated by SDS-PAGE method was about 2.51 x 10⁴daltons. CyMV-K, CyMV-A, CyMV-J was seorlogically identical by means of agar gel double diffusion test and immunosorbent electron microscopy.

뇌 동맥류성 지주막하출혈 환자간호의 질 평가 도구 개발

배소현,신성례 성인간호학회 2003 성인간호학회지 Vol.15 No.4

Purpose: The purpose of this study was to develope a evaluation tool of the quality of nursing care for aneurysmal subarachnoid hemorrhage patients at Hunt & Hess Grade Ⅰ∼Ⅲ. Method: The data was gathered from April 15, 2002 to June 14, 2002. The tool proceeded in six steps according to Bloch's framework. Result: The results of this study are as follows: 1) The quality evaluation tool for neurosurgery ICU consisted of 25 standards and 138 criteria. The quality evaluation tool for ward consisted of 27 standards and 144 criteria.; 2) The content validities of the tools for neurosurgery ICU and ward were 0.96 and 0.98, respectively.; 3) The Pearson coefficients for evaluating the interrated reliability of the tools for neurosurgery ICU and ward were r=0.9106 (p< 0.1 ) and r=0.8807 (p<0.1), respectively. 4) The ranges of corrected item-total correlations for evaluating the construct validity of the tools for neurosurgery ICU and ward were 0.0279∼0.9354 and 0.017 4∼0.9682, respectively.: And 5) The ranges of Cronbach's a reliabilities for evaluating the internal consistency reliability of the tools for neurosurgery ICU and ward were 0.6040-0.8554 and 0.6212-0.9584, respectively. Conclusion: The tools developed in this study seem useful in evaluating the quality of nursing care for aneurismal subarachnoid hemorrhage patients at Hunt & Hess Grade Ⅰ∼Ⅲ.

청각 이미지와 의복 이미지의 관련성에 대한 탐색 :성격, 성별,의복관심의 영향을 중심으로

배수정;고진아;박소현;이규혜;추호정 한양대학교 2007 韓國 生活 科學 硏究 Vol.27 No.1

This study exploratory examined the relationship between auditory images and clothing style images of young consumers by their personality types, sex and clothing interest. A questionnaire was developed to measure auditory images, clothing style images, personality types, clothing interest and demographic variables. Scale items were adopted from prior studies on clothing self images and Myers-Briggs Type Indicator (MBTI) known to measure psychological types. 120 undergraduate students with diverse majors were the respondents of the empirical study. MBTI categorized respondents into one of 3 personality types, IFT (Introversion and Thinking or Feeling, n=23), EF (Extroversion and Feeling, n=73) and ET (Extroversion and Thinking, n=24). ANOVA results indicated that, among 13 auditory images measured in the study, rational, self-confident and social images were significantly different across three personality type groups. Overall, ET group showed higher level of the the auditory images. Among clothing style images, social images were significantly different. IET group showed significantly lower level of social images. In addition, respodents' age and level of clothing interest influenced auditory and clothing style images. Correlation coefficients indicated that there were significant relationships between most auditory and clothing style images included in the study except for the self-confident image.

상심실성 빈맥환자에서 관상정맥동의 형태비교

현대우,김윤년,박소영,한성욱,허승호,김기식,김권배 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.4

재원기간중 사망한 급성 심근경색증 환자의 임상적 특징

현대우,김기식,신이철,박소영,배장호,한창엽,김윤년,김권배 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

NIHSS 활용을 통한 뇌졸중 환자 간호의 질향상 전략

박경현,송경애,공혜성,나화주,유수경,박소영,김상희,최혜림,배희준,한문구,양미화 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.2

문제: 뇌졸중 환자를 돌보는 간호현장에서는 급성기 뇌졸중 환자의 증상악화에 대한 조기사정과 적절한 대처가 매우 중요한 문제이다. 그러나 임상현장에서는 이를 객관화하고 수치화하여 모든 의료진이 의사소통할 수 있는 유용한 도구를 사용하고 있지는 못하는 실정이다. 이러한 문제를 해결하기위해 NIHSS(National Institute of Health Stroke Scale)를 신경과 진료의사를 중심으로활용하고 있지만 아직 간호현장에서는보편적으로사용하지 않고있다. 이에 뇌졸중환자간호에 NIHSS를 활용하여 뇌졸중 증상악화의 조기사정과 빠른 대처로 궁극적으로는 뇌졸중환자 간호의 질을보다 향상시키기 위하여 본활동이 시도되었다. 목적: 첫째, 간호사의 NIHSS 측정 신뢰도의 현수준을 점검하고 이를향상시키기 위한 프로그램을 개발·적용한다. 둘째, 간호사가급성기 뇌졸중환자에게 NIHSS 측정을 통하여 뇌졸중악화를 얼마나 예측할 수 있는지 분석하고 이를 향상시킬수있는 방법을 모색한다. 셋째, 궁극적으로 급성기 뇌졸중 환자의 증상악화의 조기 발견과치료를위한의료진의 올바른 대처지침을 마련하여 이를 적용한다. 의료기관: 경기도에 소재한 대학병원의 뇌졸중 집중치료실 질 향상 활동: 첫째, 간호사의 NIHSS 측정 신뢰도 향상을 위해 「NIHSS 간호사 교육 프로그램」을 마련하였다.

간세포암환자에서 간동맥 화학 색전술 후 발생한 리피오돌에 의한 폐렴 1예

김소이,김유리,허현미,배서은,이명원,최윤정,김고흔,김태헌,유 권,문일환 이화여자대학교 의과대학 2009 EMJ (Ewha medical journal) Vol.32 No.2

Hepatocellular carcinoma(HCC)is one of common causes of cancer-related death in Korea where the majority of HCC patients were Hepaitc B virus(HBV)carriers and have cirrhosis. Transarterial chemoembolization(TACE)is commonly applied to the treatment of multinodular HCC in Korea and careful selection of candidate is important for the risk of various side effects. Besides common side effects as fever, nausea, abdominal pain and elevation of liver enzyme, TACE may predispose to hepatic failure, ischemic cholecystitis, pulmonary embolism, cerebral embolism and pneumonitis. In previous studies, some cases of pulmonary and cerebral embolism cases were reported but lipiodol pneumonitis after TACE was rarely reported. A 65-year-old woman with a multinodular HCC associated with HBV infection, was treated with TACE. Seven days after the procedure, nonspecific respiratory symptoms such as dyspnea and dry cough developed. Chest X-ray and chest computed tomography showed diffuse ground glass opacities in whole lung fields, suggestive of lipiodol pneumonitis. After several days of supportive care with steroid administration, radiologic abnormalities and subjective symptoms were much improved, considered that the disease was compatible with lipiodol pneumonitis.

당뇨병 환자의 안면부 파라핀 주사부위에 발생한 피부 모균병

김종현,황소민,전재용,배용찬 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Mucormycosis is an opportunistic fungal infection caused by the mucorales, which consist of the Rhizophus, Absidia, Mortierella, and Mucor. The diverse clinical forms of mucormycosis can be categorized as rhinocerebral, pulmonary, disseminated, gastrointestinal, cutaneous and miscellaneous. Although it can cause disease in healthy people, it most commonly affects patients who are diabetic or immunosup-pressed. Rarely, mucormycosis develops confined to the subcutaneous tissue. We experienced a case of cutaneous mucormycosis developed in a well-controlled diabetic patient who had previously injected foreign material on her face for an aesthetic purpose at the behest of an unlicensed physician. The patient had tissue necrosis and purulent discharge on her left cheek. Following histologic examination, we diagnosed the condition as cutaneous mucormycosis and treated it by wide excision combined with amphotericin B therapy. The disease was cured without any significant sequelae within 6 months of follow up. Cutaneous mucormycosis is rarely reported but it can cause facial deformity due to tissue necrosis, so that early diagnosis and appropriate treatment are important. Currently, foreign material injection for aesthetic purposes is still performed by unlicensed physicians. We present a case report concluding that one should consider the possibility of fungal infection in skin lesion in diabetic or other immunosup-pressed patients. We also emphasize the seriousness of illegal injection of foreign material.

유산균들의 콜레스테를 저하성, 내산성, 내담즙성, 항생제 내성 비교

박소영,고영태,정후길,양진오,정현서,김영배,지근억 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

기능성 요구르트의 제조에 사용되는 유산균은 인체에 유익한 생리활성과 우수한 생존능력을 보유하고 있는 것이 바람직하다. 본 연구에서는 다양한 유산균주의 산과 담즙 및 항생제에 대한 내성을 조사하였으며 in vitro에서 콜레스테롤 저하능을 조사하였다. In vitro에서 콜레스테롤 저하능이 우수한 13균주를 선발하여 이들의 동결건조 분말을 고 콜레스테롤 식이의 실험쥐에 투여하였다. 그 결과 8균주는 비 투여구보다 유의적으로 18.3~27.3%의 콜레스테롤 저하능을 나타냈다(P≤0.05). 이들 중에서 Bifidobacterium infantis AM-220, Lactobacillus AM-245, Streptococcus MA-1의 3균주를 이용하여 요구르트를 제조한 후 동결건조 분말을 이용하여 급여실험을 수행한 결과, 총 콜레스테롤과 LDL 함량이 유의적으로 감소하는 것으로 나타났다. For a probiotic yoghurt it is desirable to utilize lactic acid bacteria with a high survival rate and beneficial function to human beings. We have examined a variety of lactic acid bacteria to assess the acid and bile tolerance and antibiotic resistance. In addition, an in vitro culture experiment was performed to evaluate their ability to reduce cholesterol levels in the growth medium. Thirteen strains were selected from in vitro cholesterol assays and fed to Sprague-Dawley rats with a high-cholesterol diet. Among the 13 strains tested, 8 strains were shown to reduce serum cholesterol levels significantly after 24 days of administration in vivo. Rats were fed lyophilized yoghurt powder fermented with a combination of 3 selected strains: Bifidobacterium infantis AM-220, Lactobacillus AM-245, and Streptococcus MA-1. The levels of total cholesterol and low density lipoprotein were significantly lower (p≤0.05) in rats fed the yoghurt powder compared with control group. These studies suggest that yoghurt fermented with appropriately selected lactic acid bacteria may have a anticholesterolemic effect.

골격근 근형질세망의 ATP유도 ^45Ca-uptake에 대한 Thapsigargin및 Vanillylnonanamide의 영향

황의강,배소현,홍장희,허강민,김진회,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

To investigate the effect of thapsigargin(THP) and vanillylnonanamide(VN), derivative of capsaicin, on the ATP-induced ^45Ca-uptake in the sarcoplasmic reticulum(SR) of the skeletal muscle, the SR vesicles were prepared from the back muscle of the rabbit, and ^45Ca-uptake was carried out. The results as follows: ATP-induced ^45Ca-uptake of skeletal muscular SR was significantly increased by 1 mM ATP. It was significantly blocked by 5 μM THP, but slightly decreased by 20 μM VN. The combined effect of THP and VN on the 45Ca-uptake of the SR vesicles was much potentiated than the sum of each effect of them. The above results suggest that the action of VN, being slightly influential to ATP-induced Ca-uptake but potentiating the effect of THP on the Ca-uptake, might be related with direct perturbation of the SR membrane or exposure of the THP-binding site.