비정상적인 세포증식이 유도된 혈관 내피세포에서 Protein Kinase C에 대한 활성 분석

배용찬,박숙영,남수봉,문재술,최수종 대한성형외과학회 2007 Archives of Plastic Surgery Vol.34 No.1

Purpose: To understand the pathogenesis of the disease that presents abnormally proliferated vascular endothelial cells, a model of DMH(1,2-dimethylhydrazine)-induced abnormal proliferation of HUVECs(Human Umbilical Vein Endothelial Cells) was made. We indirectly determined that Protein Kinase C(PKC) restricts the cellular proliferation and inhibits the manifestation of growth factor by using several inhibiting substances of the transmitter through our previous studies. Thereupon, we attempted to observe direct enzymatic activities of PKC and its correlation with the abnormal proliferation of vascular endothelial cells.Methods: 105 HUVECs cells were applied to 6 individual well plates in three different groups; A control group cultured without treatment, a group concentrated with 0.75×10-8M DMH only, and a group treated with DMH & 5×10-9M Calphostin C, inhibitor of PKC. In analyzing the formation of intracellular PKC enzyme, protein separation was performed, and separated protein was quantitatively measured. PKC enzyme reaction was analyzed through Protein Kinase C Assay System (Promega, USA), and the results were analyzed according to Beer's law.Results: Enzymatic activity of PKC presented the highest in all reaction time of a group concentrated only with DMH, and the lowest in the control group. The group treated with DMH and the inhibitor revealed statistically lower enzymatic activity than group only with DMH in all reaction time, although higher than the control group.Conclusion: From the enzymatic aspect, most active and immediate reaction of the PKC was observed in the group concentrated with DMH only. The group treated with DMH & PKC inhibitor showed meaningful decrease. Accordingly, PKC holds a significant role in DMH-induced abnormal proliferation of vascular endothelial cells.

양측 구순비변형 환자의 이차성 교정에 있어서 V-Y 전진 피판술 또는 정중 구순 피판술을 이용한 비주 연장술

배용찬,문재술,김상호,남수봉,강영석 대한성형외과학회 2005 Archives of Plastic Surgery Vol.32 No.5

Even though it is generalized to perform synchronous lip and nasal correction, there are some cases in need of secondary correction of cleft lip nose deformity. In these procedures, the lengthening of columella plays an important role. We performed eighteen cases of the secondary cleft lip nose deformity correction using two different methods from 1997 to 2003. The central lip flap was used in eight patients and V-Y advancement flap in ten patients. Additional procedures including reverse U-incision, interdomal fixation sutures and suspension sutures were used for correction of combined deformity. Silastic nasal retainers were kept in all patients for 6 months. Both of central lip flap and V-Y advancement flap seems to be a good technique for lengthening columellar soft tissue. But new columella after V-Y advancement flap appeared to be too narrow and a bit unnatural looking and central lip flap left additional scar on the upper lip although it was conspicuous. We think that central lip flap is a better technique in a case with wide philtrum and narrow columella and V-Y advancement flap can be another choice in a columella with sufficient width.

양성종양절제와 동시에 시행한 유방축소술

배용찬,최치원,남수봉,허재영,강영석,김영대 대한미용성형외과학회 2005 Archives of Aesthetic Plastic Surgery Vol.11 No.2

The purpose of study was to retrospectively assess the possibility of simultaneous breast reduction and tumor excision in women with benign tumor and mammary hyperplasia. Performance status of the patient, appropriate operation technique and postoperative results were analyzed. Evaluation was made regarding four patients who underwent simultaneous breast reduction and tumor excision with more than 6 months follow up. The types and locations of the tumor, corresponding operation technique, postoperative complication such as flap necrosis, subjective alteration in sensorium around the nipple-areolar complex and esthetic satisfaction were assessed.Preoperative radiologic examinations, histologic findings of the fine needle aspiration, and postoperative biopsy were equivalent. The histologic types revealed were tubular adenoma, leiomyoma and lipoma(2 cases). The tumor was each located in lower lateral quadrant, subpectoral, inframammary fold, and upper lateral quadrant of breast, respectively. Regarding the location of tumor and status of mammary hyperplasia, each breast reduction was done with superior, inferior and medial pedicle(2 cases). There were no postoperative complications and esthetic satisfaction rate was high in all patients.When accurate diagnosis based on preoperative radiologic and histologic evaluation and appropriate selection of operative technique were done, simultaneous breast reduction with excision of benign tumor showed satisfying outcome without postoperative complication even along with esthetic satisfaction.

육안상 구개열이 없는 구개인두기능부전 환자의 술후 발음 개선

배용찬,강철욱,남수봉,허재영,강영석 대한성형외과학회 2006 Archives of Plastic Surgery Vol.33 No.2

The velopharyngeal dysfunction usually occurs in patients with previous operation of the cleft palate or with submucosal cleft palate. In case of velopharyngeal dysfunction without cleft palate, no study has been made when it comes to operative method and postoperative results. Here, we would like to present the operative methods and the postoperative results with the cases we've experienced. This study is based on seven cases of velopharyngeal dysfunction without cleft palate from 1999 to 2004. Analysis of age, sex, etiology, operative methods, satisfaction rate and speech evaluation was done. The patients were 3 males and 4 females, with an age ranged from 10 to 28 at the time of surgery. The follow-up period was more than six months. One case had bifid uvula, another had atypical anomaly in palate, and five cases had no anatomical abnormality. The palatal lengthening was done on one patient, the levator muscle repositioning on another patient and to the rest of them, the superiorly based posterior pharyngeal flap was done. It was difficult to determine the etiology of the velopharyngeal dysfunction without cleft palate. The speech improvement and the satisfaction rate of the patients and parents were diverse. Although the authors had a problem with statistical analysis between the operative age and the speech improvement, it was reasonable to perform a surgical operation because postoperative speech improvement was observed in most cases regardless of age. There is little statistical correlation, but significantly higher outcomes were observed in palatal lengthening and levator muscle repositioning than in pharyngeal flap.

비중격 연골-점막 복합조직이식과 상안검 피판을 이용한 하안검의 재건

배용찬,김상호,황소민,김기태,오창근 대한성형외과학회 2004 Archives of Plastic Surgery Vol.31 No.3

Although various procedures for reconstructing the lower eyelid have been reported, their basic principle is always to build a new eyelid that is composed of three elements: an outer cutaneous layer, an inner mucosal layer, and a semirigid supporting tissue between the two layers. Mustard cheek rotation flap over a chondromucosal graft has been used often to reconstruct the full thickness defect of the lower eyelid. However, Mustard cheek flap leaves a large unacceptable scar on the face. Therefore we have repaired three cases of lower eyelid defect after resection of the basal cell carcinoma using monopedicled or bipedicled upper eyelid musculocutaneous flap over a septal chondromucosal composite graft. In addition, in case of wide defect including overall tarsal plate, the authors increased indication of mucochondral composite graft by halving method of mucochondral composite tissue. As a result, we made a minimal donor site scar and get the effect of upper blepharoplasty as well by using upper eyelid musculocutaneous flap. Based on follow-ups of 10 to 19 months, the functional and aesthetic results were found to be good in all cases. This procedure may be applicable for total or subtotal reconstruction of the lower eyelid.

Q-Switched Alexandrite 레이저(755nm)를 이용한 문신의 치료

배용찬,서창덕 釜山醫師會 1995 釜山醫師會誌 Vol.31 No.11

미국 배심재판제도에서의 배심원 선정 방법

배용찬 대검찰청 2010 형사법의 신동향 Vol.0 No.28

우리나라에서도 국민참여재판’이라는 이름으로 배심재판적 요소를 갖춘 새로운 재판제도가 도입되었으므로 선진국의 배심재판제도를 살펴보는 것이 우리제도의 발전과 성숙을 위하여 필요하다. 배심재판의 경험이 많은 미국 배심재판제도 중 배심원 선정 방법을 살펴보는 것은 공정한 재판이라는 관점에서 검찰과 피고인측 및 법원 모두의 이익을 위하여 매우 중요하다. 미국의 배심재판에서 배심원은 관할 구역내의 시민 중에서 배심원후보예정자 명단을 만들고 그 중에서 다시 자격이 있는 배심원후보자를 뽑아 법정에서 배심원후보자를 상대로 공정한 재판을 할 수 있는지, 편견은 없는지 등에 대하여 법원과 검사, 변호인이 질문하고 배심원후보자가 답변하는 절차를 거친 후 기피신청에 의해 배심원으로 선정되게 된다. 이런 복잡한 과정을 거쳐 선정된 배심원이 유무죄라는 사실관계에 대한 결정을 하게 되므로, 검사나 피고인 또는 변호인의 입장에서는 배심원을 어떻게 선정할 것인가 그리고 배심원후보자 중 누구를 배심원에서 배제할 것인가 하는 점에 지대한 관심을 가지게 된다. 이것이 배심원 선정과정에서 중요시 되는 배심원후보자에 대한 질문절차(Voir Dire)와 기피(challenge)이다. 배심원후보자에 대한 질문절차에서는 어떤 내용의 질문을 어떻게 하여 배심원후보자들로부터 공정한 재판을 할 수 있는지에 대한 정보를 얻어내고, 이를 바탕으로 어느 배심원후보자를 기피절차를 통해 배제할 것인지 결정하게 된다. 배심원후보자에 대한 질문은 편안한 분위기에서 배심원후보자들이 말할 수 있는 분위기를 조성하는 것이 제일 중요한데 이를 위해서는 개방적인 질문 등 질문기법과 내용을 검토할 필요가 있다. 최근에는 이러한 절차와 관련하여 사생활의 보호 문제와 무이유부 기피(peremptory challenge)제도를 유지할 것인지가 논의되어 왔다. 이런 점들을 소개함으로써 작년에 처음으로 도입된 국민참여재판이 우리나라에 정착되어 활성화될 뿐만 아니라 향후 예정된 국민참여재판 제도 정비에 도움이 될 수 있도록 하고자 한다.

액취증 치료에서 피부밑 삭제술과 초음파 지방흡입술의 비교

배용찬,최치원 부산대학교 병원 암연구소 2006 부산대병원학술지 Vol.- No.20

Purpose: Axillary osmidrosis develops in young people caused by the excessive secretion of apocrine gland after the adolescent period and can cause difficulty in social activities and personal relationships. Two surgical treatment methods for osmidrosis, subdermal shaving and ultrasound assisted liposuction were compared to evaluate the difference in treatment outcome. Methods: 78 patients received operation from January, 1999 to January, 2006, of whom 58 patients underwent subdermal shaving and 20 were treated with ultrasound assisted liposuction. The patients who underwent treatment using those 2 methods were compared by evaluating the time for operation and the results(odor and scar status) subjectively after surgery. Results: The satisfactory rate was 88% in 58 patients who underwent subdermal shaving and 80% in 20 patients who underwent liposuction. Mean operative time for subdermal shaving is 117 minutes and that of ultrasound assisted liposuction is 98 minutes. 3 patients, who underwent subdermal shaving, complained of hypertrophic scar on the axilla, of whom 2 patients needed scar revision. There was no hematoma, necrosis of flap and scar contracture. Conclusion: There is no significant statistic correlation between the satisfactory rate, scar formation and operative methods. But both subdermal shaving and ultrasound assisted liposuction are effective methods in treatment of osmidrosis because the postoperative satisfactory rate is high and other complications are minimal.

신호전달 경로의 저해제를 이용한 혈관 내피세포의 비정상적인 증식 기전에 대한 연구

배용찬,박숙영,남수봉,허재영,강영석 대한성형외과학회 2006 Archives of Plastic Surgery Vol.33 No.1

Protein tyrosine kinase(PTK), protein kinase C(PKC), oxidase, as a mediator, take a significant role in signal transduction pathway of angiogenesis. The authors utilized the inhibitors, targeting the formation of three co-enzyme in signal transduction pathway in order to quantify the suppression of abnormal vascular endothelial cell proliferation induced by DMH, to compare the level suppression in each up-regulated growth factors, CTGF, CYR61, ITGβ1, FHL2, and to identify the relationship between abnormal cell proliferation and signal transduction pathway. Five groups were established; Control group, Group of DMH, Group of DMH-mixed Herbimycin, inhibitor of protein tyrosine kinase, Group of DMH-mixed Calphostin C, inhibitor of protein kinase C, Group Of Dmh-Mixed 10U Catalase, Inhibitor Of oxidase. The rise of vascular endothelial cell was compared by MTT assay, and four growth factors were analysed with RT-PCR method, at pre-administration, 4, 8, 12, and 24 hours after administration. In comparison of abnormal proliferation of vascular endothelial cell induced by DMH, suppression was noticed in Herbimycin and Calphostin C group, and Calphostin C group revealed higher suppression effect. Nevertheless, Catalase group did not have any suppression. In manifestation of four growth factors, Herbimycin and Calphostin C group presented similar manifestation with control group, except in ITGβ. Catalse group had similar manifestation with DMH group in all four growth factors. Abnormal proliferation of vascular endothelial cell induced by DMH have a direct relationship with PTK and PKC, more specifically to PKC. Oxidase was confirmed not to have any relevance.

고막 운동성 계측에 의한 구개열 환자에서의 중이염 유병률

배용찬,김상호,황소민,김기태,남수봉,전경명,고의경,이일우 대한성형외과학회 2004 Archives of Plastic Surgery Vol.31 No.5

There is no dispute on that the ear evaluation should be done continuously for cleft palate patients. But, there is a controversy about interrelation between otitis media and clinical factors. Therefore, we have analyzed the relationship between the prevalence of otitis media and several prospective clinical factors. In this study, 96 cleft palate patients(191 hearing ears with one atresia) were examined to evaluate middle ear function by tympanometry. In all cases, middle ear evaluation was performed before subsequent palatoplasty and then, the middle ear evaluation was performed again in 145 ears, at an average of 5.7 months after the procedure. The prevalence before palatoplasty in cleft palate patients shows that otitis media with effusion is present in 60 percent, and otopathologic feature tends to decrease with age. The prevalence of otitis media in submucous cleft palate was lower than that in incomplete or complete cleft palate patients, and the types of cleft palate seems statistically not related to any incidence of otitis media. There was statistical reduction in the prevalence of otitis media after palatoplasty. Our research indicates that the prevalence of otitis media in cleft palate patients was reduced by increased age and palatoplasty. Otological check up should be done continuously for the cleft palate patients.