견관절 주위 원발성 골 종양에서 사지 구제술의 종양학적 결과 및 기능적 평가

이상훈,유재호,오주한,서성욱,김한수,Lee, Sang-Hoon,Yoo, Jae-Ho,Oh, Joo-Han,Suh, Sung-Wook,Kim, Han-Soo 대한근골격종양학회 2002 대한골관절종양학회지 Vol.8 No.3

목적: 견관절 주위 원발성 골 종양 환자에서 시행한 사지 구제술의 종양학적, 기능적 결과를 평가하고자 하였다. 대상 및 방법: 1982년에서 2001년 사이에 견관절 주위 원발성 골 종양으로 본원에서 사지 구제술을 시행 받은 환자 중 추시 관찰이 가능한 2 9명을 대상으로 하였다. 평균 연령은 35세, 평균 추시 기간은 7년 1개월(1년 2개월~19년 9개월)이었고, 골육종 7예, 연골육종 14예, 방골성 골육종 1예, 혈관외피종 1예, 거대세포종 6예였다. 사지 구제술의 방법으로는 시멘트 충전술을 7예에서, 시멘트 주형 관절성형술 및 골수강내 정 삽입술을 10예에서, 종양 대치물 삽입술을 7예에서 이용하였으며, 5예에서는 절제만 시행하거나, 골이식, 관절 유합술 등을 실시하였다. 술 후 기능 평가는 근골격계 종양 학회(Musculoskeletal Tumor Society)의 상지 근골격계 종양 환자 기능 평가 방법을 이용하였다. 결과: 추시 도중 6예에서 국소 재발 하였고, 2예에서 폐 전이로 재발 하였고, 2예에서는 국소 재발과 폐전이가 모두 있었으며 3명이 사망하였다. 구제된 상지의 생존율은 최종 추시상 88.6%였으며, 재발이나 전이가 있을 경우 열등한 사지의 생존율을 보였다(p=0.012). 근골격계 종양 학회 기능 평가상 구제된 상지의 기능 점수는 평균 80%(24점, 18~30점)였다. 치료방법에 따른 기능 점수는 시멘트 충전술 86%, 시멘트 주형 관절성형술 및 골수강내 정 삽입술 71%, 종양 대치물 삽입술 83%점으로 시멘트 주형 관절성형술 및 골수강내 정 삽입술이 통계적으로 유의하게 좋지 않은 결과를 보여주었다(p=0.034). 합병증으로 요골 신경 마비와 액와 신경 마비가 각각 1예 있었으며, 생 비골 이식편의 이탈로 재수술을 받은 경우가 1예, 창상 감염이 1예 있었다. 결론: 견관절 주위 악성 골 종양과 거대 세포종의 치료에 있어서, 사지 구제술은 종양학적, 기능적으로 만족할 만한 결과를 얻을 수 있는 방법으로 사료된다. Purpose: The purpose of this study was to assess the oncologic results and functional outcomes of limb salvage surgery performed in patients of primary bone tumors of the shoulder girdle. Materials and Methods: Twenty-nine patients who underwent limb sparing resection for shoulder girdle neoplasm between 1982 and 2001 were analyzed. Follow up periods averaged 7 years and 1 month. Mean age of the patients was 35 (11~71) years. There were 14 males and 15 females. Primary malignant bone tumors of shoulder girdle (proximal humerus 21, scapula 3, both 1) were 23 cases; osteosarcomas 7, chondrosarcoma 14, parosteal osteosarcoma 1, hemangioendothelioma 1, and giant cell tumor of proximal humerus were 6 cases. Limb salvage surgery was performed by curettage and cementing in 7 patients, by cement molding arthroplasty in 10 patients, and by tumor prosthesis in 7 patients, by other method such as resection only, bone graft, arthrodesis in 5 patients. The Musculoskeletal Tumor Society functional rating system was used to assess functional outcomes. Results: One osteosarcoma and 2 chondrosarcoma patients died, and the survival of the salvaged limb was 88.6% at the final follow-up. There were 6 local recurrences, 2 lung metastases, 2 local recurrences and lung metastases. The functional outcome was 80%. There was statistically significant difference of functional results among the patients treated by cement filling (86%), cement molding arthroplasty and IM nailing (71%), and tumor prosthesis (83%). (p=0.034) There were three complications including 1 radial nerve palsy and 1 axillary nerve palsy, and 1 wound infection. Dislodgement of vascularized fibular graft in one patient was treated by internal fixation. Conclusion: Limb salvage surgery seems to be useful method to treat bone tumors of the shoulder girdle.

Development of Quantitative Vitellogenin ELISAs for Bullfrog (Rana catesbeiana) used in Endocrine Disrupter Screening

이상훈,강윤주,이춘일,김안드레,김춘봉,정규혁,김동규,박남규,박광식,강신원,박장수,Lee Sang-Hoon,Kang Yun-Ju,Li Chun-Ri,Kim Andre,Jin Chun-Feng,Chung Kyu-Hyuck,Kim Dong-Kyoo,Park Nam-Gyu,Park Kwang-Sik,Kang Shin-Won,Park Jang-Su The Korean Society of Environmental Toxicology 2006 환경독성보건학회지 Vol.21 No.2

난생 생물의 알 생성유도 단백질인 비텔로제닌(viteILogenin, VTG)을 성숙한 암컷 황소개구리 (Rana catesbeiana)혈청으로부터 음이온 교환 크로마토그래피를 이용하여 정제 하였으며 정제한 비텔로제닌을 BALB/c mice에 주사하여 폴리크로날 항체를 생산하였고 이것은 protein A column으로 정제 하였다. 이렇게 정제된 폴리크로날 항체를 이용하여 황소개구리 비탈로제닌 측정용 효소면역측정법을 개발하였으며 그 측정 범위는 $12{\sim}1,560ng/mL$였다. 또한 이 효소면역측정법을 평가하기 위해 성숙한 수컷 황소개구리를 청정지역과 폐수처리장 하류 하천에서 서식하는 황소개구리 혈액 속의 비텔로제닌을 측정하였다. 그 결과 폐수처리장 하류 하천에 서식하는 수컷 황소개구리 비텔로제닌이 청정지역보다 현저하게 높게 유도됨을 알 수 있었다.

TinyDB와 라인트레이서를 활용한 TinyOS기반의 센서 데이터 처리 모듈 설계 및 구현

이상훈,문승진,Lee, Sang-Hoon,Moon, Seung-Jin 한국통신학회 2006 韓國通信學會論文誌 Vol.31 No.10B

유비쿼터스 컴퓨팅 기술에 대한 관심이 하드웨어, 통신, 데이터베이스 등의 다양한 분야에서 매우 높게 나타나고 있음에 따라 무선 센서 네트워크에 대한 연구가 활기를 띠고 있다. 특히 최근에 새로 보급되는 스마트 센서들은 각 센서 노드들이 정보를 실시간으로 수집하고 분석할 수 있어, 이에 따른 데이터 처리 역시 중요한 이슈로 부각되고 있다. 본 논문에서는 이동 경로를 주행할 수 있는 라인트레이서를 설계 및 구현하여 쿼리 프로세싱 시스템인 TinyDB를 활용해서 라인트레이서에 센서 노드를 탑재하여 이동 경로 주변의 데이터를 수집하였고, 정지해 있는 특정한 위치에서의 데이터와 비교하였으며, 수집된 데이터를 저장하여 웹 서버에서 나타내었다. 또한 사용자의 편의를 위해 임베디드 보드에서 웹 서버로의 접근을 통해서 터치 식으로 쉽게 데이터를 볼 수 있도록 구현하였다. The study of sensor network database is beginning to liven up as we are interested in Ubiquitous Computing technology in hardware, communication, database and so on. Especially, as new smart sensors have capabilities of real-time information gathering and analysis of each sensor node, data processing becomes an important issue in Ubiquitous Computing. In thesis, we have applied TinyDB(query processing system) to carry sensor node with line tracer which can follow the fixed path. After we gathered data around path, we have processed data in TinyDB GUI, gathered data, displayed data on a web server. Also we have a web browser on an embedded board for convenient user interface and implemented touch screen such that users can operate with a finger.

크기 조절이 가능한 은 나노입자 형성을 위한 박막의 열처리 효과

이상훈,이태일,문경주,명재민,Lee, Sang Hoon,Lee, Tae Il,Moon, Kyeong-Ju,Myoung, Jae Min 한국재료학회 2013 한국재료학회지 Vol.23 No.7

In order to produce size-controllable Ag nanoparticles and a nanomesh-patterned Si substrate, we introduce a rapid thermal annealing(RTA) method and a metal assisted chemical etching(MCE) process. Ag nanoparticles were self-organized from a thin Ag film on a Si substrate through the RTA process. The mean diameter of the nanoparticles was modulated by changing the thickness of the Ag film. Furthermore, we controlled the surface energy of the Si substrate by changing the Ar or $H_2$ ambient gas during the RTA process, and the modified surface energy was evaluated through water contact angle test. A smaller mean diameter of Ag nanoparticles was obtained under $H_2$ gas at RTA, compared to that under Ar, from the same thickness of Ag thin film. This result was observed by SEM and summarized by statistical analysis. The mechanism of this result was determined by the surface energy change caused by the chemical reaction between the Si substrate and $H_2$. The change of the surface energy affected on uniformity in the MCE process using Ag nanoparticles as catalyst. The nanoparticles formed under ambient Ar, having high surface energy, randomly moved in the lateral direction on the substrate even though the etching solution consisting of 10 % HF and 0.12 % $H_2O_2$ was cooled down to $-20^{\circ}C$ to minimize thermal energy, which could act as the driving force of movement. On the other hand, the nanoparticles thermally treated under ambient $H_2$ had low surface energy as the surface of the Si substrate reacted with $H_2$. That's why the Ag nanoparticles could keep their pattern and vertically etch the Si substrate during MCE.

생명공학과 의학을 위한 마이크로 기술

이상훈,Lee, Sang-Hoon 대한의용생체공학회 2008 의공학회지 Vol.29 No.1

Recent a few decades, the microtechnology has been progressed so rapidly and applied in diverse areas. Especially, this technology was focused on the field of biotechnology and medicine because of its size and simple fabrication process. In this paper, the current status of microtechnology is briefly introduced from the aspect of material, process and device and the application of this technology in biotechnology and medicine is also described. The microtechnology will be more broadly applied in future in the biotechnology and medicine area and the biomedical engineer should have continuous interests in this technology.

DME 연료의 분무 특성에 관한 연구

이상훈,전문수,Lee, Sang Hoon,Chon, Mun Soo 한국교통대학교 융복합기술연구소 2013 융ㆍ복합기술연구소 논문집 Vol.3 No.2

This paper describes the atomization characteristics, as well as the velocity and size distribution, of DME spray based on common-rail injection system. To analyze the possibility of using DME fuel as an alternative fuel of diesel, spray atomization characteristics were investigated. For this investigation, two-dimensional phase Doppler analyzer system was used to obtain droplet size and velocity distribution simultaneously. Velocity and droplet size measurements were performed at various injection pressures. Results showed that increasing pressure from 25MPa to 50MPa leads to higher spray droplet velocities and smaller droplet diameter but injection pressure above 40MPa, no signifiant reduction was observed. With the droplet velocity and SMD comparison between diesel and DME fuel, it can be observed that DME has smaller SMD and droplet velocity due to its low surface tension.

국내육성 톨 페스큐 품종간 생육특성 및 종자생산성

이상훈,최기준,김기용,지희정,황태영,박형수,김종근,이기원,Lee, Sang-Hoon,Choi, Gi Jun,Kim, Ki-Yong,Ji, Hee Chung,Hwang, Tae Young,Park, Hyung Soo,Kim, Jong Gun,Lee, Ki-Won 한국축산환경학회 2014 한국축산시설환경학회지 Vol.20 No.4

A new tall fescue variety (Festuca arundinacea Schreb.) named Greenmaster and Purumi were. In this study, we evaluated the seed production of new tall fescue domestic varieties were cultivated in Korea. Agronomic aspects of tall fescue domestic varieties were determined using seed production and forage nutritive value of straw at the National Institute of Animal Science from 2013 to 2014. The heading date according to the varieties displayed differences and domestic varieties showed enhanced disease resistance. The harvested seed yields of Greenmaster was 35% higher as 1,664 kg/ha and it's also yield of straw higher than that of Fawn. However, the nutritive value of both varieties was similar. The dry matter yield of Pumumi was 25% higher as 11,080 kg/ha. Accordingly, developed a new variety of domestic tall fescue with excellent environmental adaptability, aiming to make contribution to the vitalization of the Korean grassland industry.

오차드그래스 성숙종자로부터 캘러스 유도 및 고효율 식물체 재분화

이상훈,이동기,김진수,이병현,Lee, Sang-Hoon,Lee, Dong-Gi,Kim, Jin-Soo,Lee, Byung-Hyun 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.4

In an effort to optimize tissue culture conditions for genetic transformation of orchardgrass (Dactylis glomerata L.), an efficient and high-frequency plant regeneration system from seed-derived calli was established. Embryogenic calli induced on MS medium containing 3mg/L 2,4-D and 0.1mg/L BA had significantly improved regeneration ability. Plant regeneration rate was 92% when embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among three kinds of medium, MS and N6 medium were optimal for embryogenic callus induction and plant regeneration, respectively. Ho difference in callus induction frequency was observed among four cultivars of orchardgrass, however, "Roughrider" cultivar showed higher regenerability with the frequency of 61%. Addition of maltose to the regeneration medium as a carbon source dramatically increased regeneration frequency up to 69%. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of orchardgrass through genetic transformation.

코발트-크롬 합금의 표면처리에 따른 열중합형 의치상용 레진과의 전단결합강도

이상훈,황선홍,문홍석,이근우,심준성,Lee, Sang-Hoon,Hwang, Sun-Hong,Moon, Hong-Seok,Lee, Keun-Woo,Shim, June-Sung 대한치과보철학회 2007 대한치과보철학회지 Vol.45 No.2

Statement of problem: For the long-term success of removable partial dentures, the bonding between metal framework and denture base resin is one of the important factors. To improve bonding between those, macro-mechanical retentive form that is included metal framework design has been generally used. However it has been known that sealing at the interface between metal framework and denture base resin is very weak, because this method uses mechanical bonding. Purpose: Many studies has been made to find a simple method which induces chemical bond, now various bonding system is applied to clinic. In this experiment, shear bond strengths of heat-cured denture base resin to the surface-treated Co-Cr alloy were measured before and after thermocycling. Chemically treated groups with Alloy $Primer^{TM}$, Super-Bond $C&B^{TM}$, and tribochemically treated group with $Rocatec^{TM}$ system were compared to the beadtreated control group. The data were analyzed with two-way ANOVA. Result: 1. Shear bond strength of bead-treated group is highest, and Alloy $Primer^{TM}$ treated group, Super-Bond $C&B^{TM}$ treated group, RocatecTM system treated group were followed. Statistically significant differences were found in each treated group(p<0.05). 2. Surface treatment and thermocycling affected shear bond strength(p<0.05), however there was no interaction between two factors(p>0.05). 3. Shear bond strengths of bead-treated group and Alloy $Primer^{TM}$ treated group showed no statistically significant difference before and after thermocycling(p>0.05), and those of Super-Bond $C&B^{TM}$ treated group and $Rocatec^{TM}$ system treated group showed statistically significant difference after thermocycling(p<0.05).

봉독약침이 류마티스 관절염 환자의 관절 통증, 종창 및 급성 염증 반응에 미치는 영향

이상훈,이현종,백용현,김수영,박재경,홍승재,양형인,김건식,이재동,최도영,이두익,이윤호,Lee, Sang-hoon,Lee, Hyun-jong,Baek, Yong-hyeon,Kim, Su-young,Park, Jae-kyung,Hong, Seung-jae,Yang, Hyung-in,Kim, Keon-sik,Lee, Jae-dong,Choi, Do-young,L 대한침구의학회 2003 대한침구의학회지 Vol.20 No.2

Objective : In order to study the effects of bee venom(BV) on the pain, edema, and acute inflammatory reactant of rheumatoid arthritis(RA) patients. Methods : Patients with RA who met the ACR(American College of Rheumatology) 1987 revised criteria for the diagnosis of RA were treated with the BV therapy twice a week for 3 months. Tender joint counts, swollen joint counts, Visual analog scale(VAS), morning in stiffness, ESR, C-reactive protein(CRP) were analyzed before and after BV therapy. Results : The results as follows: 1. Tender joint counts in patients after BV therapy were significantly lower than those before BV therapy($9.0{\pm}7.9$ vs $15{\pm}11.4$, p=0.002). 2. Swollen joint counts of the patients after BV therapy were significantly lower than those before BV therapy ($5.0{\pm}6.1$ vs $1.5{\pm}2.3$, p=0.001). 3. VAS in patients after BV therapy was significantly lower than before BV therapy($60.8{\pm}17.6$ vs $38.0{\pm}15.9$, p=0.000). 4. Duration of morning stiffness in patients after BV therapy was significantly reduced compared with that before BV therapy($119.1{\pm}112.6min$ vs $59.0{\pm}89.7min$, p=0.009). 5. ESR and CRP were not significantly changed before and after BV therapy, suggesting BV itself could make inflammatory reaction as well as therapeutic effect. Conclusions: BV therapy improved tender joint counts, swollen joint counts and duration of morning stiffness in this study, and further study is needed in log-term effect of BV therapy.