녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

성인에서 발생한 발진성 가성 혈관종증

서상희 ( Sang Hee Seo ),장호선 ( Ho Sun Jang ),목혜수 ( Hye Soo Mok ),김성준 ( Sung Jun Kim ),김병수 ( Byung Soo Kim ),김문범 ( Moon Bum Kim ),오창근 ( Chang Keun Oh ),권경술 ( Kyung Sool Kwon ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.8

Background: Eruptive pseudoangiomatosis (EPA) is a rare, benign, spontaneously regressing childhood exanthem. It is characterized by the sudden onset of several bright red angioma-like papules surrounded by pale halos with a distinct histopathology from true angiomas. Objective: This study was performed to evaluate the clinical and histopathologic characteristics of EPA occuring in adults. Methods: Ten adult patients who visited Pusan National University Hospital and Mok Hye-SooㆍJang Ho-Sun Dermatology Clinic from March 2005 to September 2006 were evaluated. We prospectively evaluated the sex, age, onset season, past medical history including immunosuppressive abnormalities, systemic disorders and other diseases including allergies. We also investigated the relations of mosquito biting, patients` occupations and outdoor activities to occurrence of EPA. In addition, simultaneous occurrence in family members, the clinical, histopathologic, laboratory findings, disease courses and responses to treatment were evaluated. Based on medical records, photographs and pathologic slides, we retrospectively diagnosed another 20 EPA patients suspected as insect bite from October 2003 to March 2005. The same questions were inquired as for the 10 patients who prospectively underwent evaluation. Results: In the study, female predominance (76.7%) was observed and the average age of onset was 54.2 years. Interestingly, there was no child patient during the period of study. Multiple, 2∼5 mm sized, red angiomatous papules surrounded by pale halos occurred on exposed areas such as the arms (86.7%), legs (50%), and face (46.7%), although it could also occur to a non-exposed area. EPA occuring in adults usually appeared in summer (80%). The mean disease duration was 3.4 weeks. Although EPA spontaneously regressed, it had the potential of recurrence (46.7%). Histopathologic findings showed dilated dermal blood vessels without the evidence of increase in numbers, and perivascular lymphocytes infiltration. Inside the lumen of dermal blood vessels, plump endothelial cells were found. Conclusion: EPA occuring in adults usually happened to exposed sites in summer, so it can be misdiagnosed as insect bite. We suggest that dermatologists should be concerned about EPA in adults and conduct further investigation to have a better understanding of the disease. (Korean J Dermatol 2007;45(8):797∼803)

鹽-觸媒와 Phenyl Glycidyl Ether에 依한 絹纖維의 改質에 관한 硏究

張炳浩,李相洛 成均館大學校 科學技術硏究所 1988 論文集 Vol.39 No.2

The silk was padded with NaSCN containing to an approximate 100% pick up and then treated with solution PGE in various solvents at 75℃ for 6 hours. The conclusions were as follows; 1) PGE add-on reached maximum when 1N NaSCN, 1.5M PGE and 100% moisture regain were used in ethanol. 2) The dry wrinkle recovery angle, chemical resistance is ere increased continuously with the increase of PGE add-on. 3) Amount of amino acid in silk was decreased as PGE add-on increased. Especially, tyrosine was decreased remarkably. 4) The effect of K/S and PGE add-on were the most outstanding when n-Hexane was used in the various solvents.

알코올 발효성 야생 효모의 선발 및 발효특성

장재호*, 한상민*, 이종수 배재대학교 자연과학연구소 2017 自然科學論文集 Vol.28 No.1

충남 예당 저수지부근 토양에서 분리한 야생효모 중 Aureobasidium pullulans 효모가 가장 발효능이 우수하여 최종 선발하였다. 20% 포도당을 함유한 YPD 배지에서 Aureobasidium pullulans의 발효 최적 조건을 조사한 결과 에탄올 함량은 25℃에서 7일 발효시켰을 때 가장 많은 12.6 v/v%의 에탄올을 생성하였다. In order to select alcohol-fermenting wild yeast, among thirteen strains of non-pathogenic wild yeasts from Yedang Reservior, Chungnam province, Korea, Aureobasidium pullulans P-1 was screened as a potent alcohol-fermenting yeast. Optimal ethanol fermentation condition was investigated using 20% glucose- 1% yeast extract-2% peptone(20 DYP) broth. Maximal 12.6 v/v% ethanol was produced when 20% DYP broth was fermented by 5% Aureobasidium pullulans P-1 culture broth at 25℃ for 7 days.

폐기물 성상과 매립방식에 따른 침출수 특성

성낙창,김은호,문추연,김정권,장성호,김수생,박출재 동아대학교 환경문제연구소 1996 硏究報告 Vol.19 No.1

In this study, we can be obtained the following conclusions about the characteristics of leachate with waste compositions and landfill methods. pH shows a tendency to increase in E. landfill, because NH₃ is created by anaerobic degradation and dissolved in leachate. The concentrations of BOD and COD become different, In case of E. landfill, food of combustible is H. & S. landfill and then SS is high in concentration. According to passed time, T-N is high in concentration, but T-P shows a similar tendency. Heavy metals of leachate is lower than threshold concentrations. If leachate is treated biologically, microbes are not inibitory.

주기억장치 데이타베이스를 위한 새로운 락 관리

장연정,김상욱,김진호,김윤호 강원대학교 정보통신연구소 1999 정보통신논문지 Vol.3 No.-

The locking is the most widely-used concurrency control mechanism for guaranteeing logical consistency of a database where a number of transactions perform concurrently. In this paper, we propose a new lock management method appropriate for main memory databases. Our method chooses the partition, a fixed-sized container for records, as a unit of locking, and directly keeps lock information within the partition itself. These make our method enjoy the following advantages: (1) it has freedom in controlling of the trade-off between the concurrency and locking overhead by considering the characteristics of given target applications, (2) it enhances overall system performance by eliminating the hashing overhead, a serious problem occurred in the traditional method.

인터벌 트레이닝이 근대 5종 선수들의 감소 트레이닝 동안 최대유산소 능력과 달리기 경기력에 미치는 영향

장경태,이상호,김영선 한국체육대학교 체육과학연구소 2001 국제학술대회논문집 Vol.20 No.1

To investigate the effects of interval training on VO2max and 3,000m running performance during reduced training, thirteen modern pentathlonists were studied before and after four weeks of training. Seven subjects continued their usual training program of 6 days per week(6D group) and six subjects followed reduced training program of 3 days per week(3D group). In the reduced training program, volume and frequency of running were reduced by 65% and 50%, respectively. However, high intensity intervals were the main part of the running in reduced program. Time to exhaustion while running at 16km/h with 10% grade, and total exercise time of VO2max test were increased in 6D group after 4 weeks of training. There were no changes in VO2max of both groups. 3,000m running time were decreased slightly in both groups but it was not significant. It was concluded that four weeks of reduced training with increased intensity maintained VO2max and 3,000m running performance of modern pentathlonists.

産業災害 發生에 미치는 勞動動向의 傾向 分析

張永鎬,車相殷 안동간호보건전문대학 1991 안동간호보건전문대학논문집 Vol.13 No.-

지식 기반 교육컨테츠 저작시스템

장재경,김호성 한국컴퓨터교육학회 2004 컴퓨터교육학회 논문지 Vol.7 No.2

e-Learning에서 교수-학습자들 간에 형성되는 지식들을 체계적으로 관리하여 효과적인 교수-학습을 수행할 목적으로 교수자들이 교수 설계 이론에 따른 다양한 강의들에 교육 컨텐츠를 보다 쉽게 저작할 수 있는 시스템을 개발하였다. SCORM 표준에 따라 학습객체 간 관계를 설정하여 저작된 컨텐츠는 학습자가 학습내용을 개념화하는데 도움을 줄 것이다. 시맨틱 웹의 RDF를 적용하여 학습객체 간의 관계를 설정함으로써 지식맵을 구성하였고, 교수자가 해당 분야에서 사용되는 단어를 등록하여 일종의 어휘사전을 만들 수 있는 온톨로지를 도입하였다. 학습자의 이해도와 성취도를 고려한 개별학습을 제공하기 위하여 도입된 상호작용점을 따라 학습자마다 개별적인 학습맵이 구성되어 학생 개인의 학습활동과 이해도를 평가할 수 있다. For the purpose of an effective instruction-learning process by systematic management of knowledge between instructor and learner in e-Learning, we have developed the authoring system in which the instructor is able to author easily on various lecture frames according to the instructional design theory. The authored contents with the relations among the learning objects based on SCORM standard would help learner to conceptualize the contents. A knowledge map is constructed on the relations among the learning objects using RDF of the semantic web. We introduce the ontology in which the instructor can make a dictionary of terminology by registering the words of the teaching area. The learning activity and comprehension of students can be assessed using each student's learning map along the interaction points which are introduced to present the individual learning by considering each student's capacity of understanding and achievement.

開化期 國語敎科書 硏究

張相浩 朝鮮大學校 敎育大學院 國語敎育學會 1981 國語敎育論叢 Vol.1 No.-