정상 한국인에서 연령증가에 따른 혈장 Dehydroepiandrosteronesulfate ( DHEA-S ) 농도 및 DHEA - S / cortisol 비의 변화

박성우,유형준,임성희,최문기,유재명,박민숙,이권엽,박철수,김철홍,김현규,김두만,박규용 대한내분비학회 1997 Endocrinology and metabolism Vol.12 No.2

Backgroud: DHEA-S is the most abundant steroid hormone in circulation, and primarily secreted from the adrenal cortex, but its physiological role is little known. One of the characteristic features of DHEA-S is progressive decrement of plasma DHEA-S level with advancing age, in contrast, plasma levels of other adrenal hormones are not chaging or littie decreasing. To grasp the trends of plasma DHEA-S level and DHEA-S/cortisol ratio by age in healthy Korean, we measured the plasma DHEA-S levels and DHEA-S/cortisol ratios in healthy Korean. Methods: Healthy Korean(men: 99, women: 102, age range: 15-97 year old)were studied. Subjects were not taking drugs(such as glucocorticoid or androgenic medication) or cigarettes known to modify the plasma level of DHEA-S and cortisol, and had no evidence of hepatic, renal disease or hyperlipidemia as determined by serum lipid, bilirubin, SGOT, SGPT, BUN, creatinine. Data were analyzed by 10-year age group for men and women: i.e, 10-19, 20-29, 30-39, 40- 49, 50-59, 60-69, 70-79, 80-89 and 90 year or more. Plasma DHEA-S levels were measured by using a commercially available RIA kit with 125I labeled-DHEA-SO4(Coat-A Count DHEA-SO4), and for the measurement of plasma cortisol levels, commercial Gamma Coat TM[125I] Cortisol Radioimmunassay Kit was used. Results:. 1) In both men and women, plasma DHEA-S level showed high interindividual variation within the same age group. 2) There were individual sex differences in plasma levels of DHEA-S, in all age groups, plasma DHEA-S levels were significantly higher values for men than for women. 3) Maximum plasma DHEA-S levels(men; 237+-3.35 ug/dL, women; 108+-17.5 ug/dL) were at third decade in both men and women. 4) Both men and women showed the continuous decline in plasma DHEA-S level with age. These age-related decline was more prominent in men than in women(men; y= -3.152 * +292.6, r2= 0.8459, P$lt;0.05, women; y= -1.417 * +143.3, r2 = 0.7278, P$lt; 0.05). 5) As an index of aging, there was no stastical difference between DHEA-S and DHEA- S/cortisol ratio. Conclusion: In healthy Korean, there were high interindividual variation of plasrna DHEA-S levels. In both men and women plasma DHEA-S level was peak at third decade, and from when it declined progressively with age. These results suggest that although the reliability of single plasma DHEA-S measurement are limited, the decline of DHEA-S with advancing age might be a specific marker of endocrinologic hormonal milieu(aging index). Also, concerning to individual adrenal secreting capacity, we measured DHEA-S/cortisol ratio. But we did not found that plasma DHEA-S/cortisol ratio is superior to the plasma DHEA-S level as an aging index. (J Kor Soc Endocrinol 12:245-254, 1997)

전자 서베잉을 이용한 서베이드 금관과 국소의치 프레임워크 디자인

홍용신,박은진,김성균,곽재영,허성주,박지만 大韓齒科保存學會 2011 Restorative Dentistry & Endodontics Vol.36 No.6

구강 스캐너와 CAD/CAM 기술로 인해 컴퓨터로 제작하는 방식이 활발히 보급된 고정식 보철 증례와는 달리, 의치를 디지털 방식으로 제작하는 데에는 아직 한계가 많다. 촉각을 느낄 수 있는 입력 장비와 전자 서베잉의 개념, 그리고 쾌속 조형법의 적용으로 인해 국소의치를 디지털로 제작할 수 있는 솔루션이 최근 소개되고 있다. 본 증례보고에서는 이러한 국소의치의 디지털 솔루션을 이용하여 총 다섯 명의 환자에서 서베이드 금관 및 국소의치를 제작하였다. 인상 과정상의 문제로 모형과 실제 치아 사이의 형태적 차이가 있었던 경우를 제외하고는 국소의치의 적합도는 우수했으며, 장착 후 안정성과 착탈 시의 유지력 또한 임상에서 적용하는 데에 문제가 없었다. There are still many limitations on fabricating dentures using digital method while computerized production of fixed prostheses utilizing intraoral scanner and CAD/CAM technology has propagated rapidly. Recently the digital solution of fabricating removable partial denture by applying haptic input device, electronic surveying, and rapid prototyping was introduced. In this case presentation, five patients were treated with surveyed crown and removable partial dentures by this digital solution. Fit of dentures was excellent except for one case which showed morphological difference between the actual teeth and that of master cast by the erroneous impression process. There also was not any problem of stability and retention after adaptation in the clinical setting.

열안정형 카테콜-O-메틸전이효소 유전자의 클로닝

홍경만,최용복,정갑용,지은정,장현신,박현,백문기 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Catechol-O-methyltransferase (COMT; EC 2.1.1.6) is the enzyme which catalyzes the transfer of methyl group to the catecholamine neurotransmitters from its methyl donor S-adenosyl-L-methionine. Partially purified COMT from rat liver has been used to measure the concentration of catecholamines in the blood through solvent extraction and thin layer chromatography after converting ^3H-methyl derivatives of catecholamines. To improve this inconvenient and fluctuating method, an attempt was made to use COMT gene for the measurement of catecholamines. Specific primers, COMT5P (5'-TGC TCA GAG GTG CTT TGA AG-3') and COMT3P (5'-GGA GCC GCA GAA GGT CA G-3'), were used to amplify COMT gene from human placenta cDNA library. The amplified COMT gene through 35 cycles of polymerase chain reaction was cloned into T-vector and the nucleotide sequences are determined by automatic sequencer. Human COMT has two common variants, a thermostable high activity form which has valine at amino acid 158 and a thermolabile low activity form which Has methionine at the same position. The cloned COMT gene in this study has both variants. Clone 4 and 5 have valine at amino acid 158 (guanine at nucleotide 472) and clone 1-3 have methionine at this site (adenine at nucleotide 472). In addition to this, there are other DNA polymorphisms in COMT gene at nucleotide 101, 102 (amino acid 34, a structural mutation cysteine/serine) and nucleotide 186 (a silent mutation). All of the clones have cysteine at amino acid 34 (guanine and adenine at nucleotides 101 and 102) and cytosine at nucleotide 186 except clone 3 which has thymine at this nucleotide. Clone 4 and 5 are thermostable high activity variants, suggesting being more useful for the measurement of catecholamines after expressing the gene.

골절의 유형과 정도에 따른 전두동 골절의 치료 : 23예의 분석결과

진홍률,신시옥,최영석,송창근,용만강 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.4

구강 내 디지털 인상채득을 통한 맞춤형 지대주와 시멘트 합착 후 나사형 임플란트 보철 수복 증례

홍용신,박은진,김선종,김명래,허성주,박지만 대한치과보철학회 2012 대한치과보철학회지 Vol.50 No.1

최근 디지털 인상채득 시스템이 개발되면서 이를 기존의 CAD/CAM 시스템과 접목한 인레이, 온레이, 크라운이나 간단한 계속가공의치 등의 고정성 보철물의 제작이 활발히 소개되고 있다. 그러나 임플란트 보철에서는 주로 기존의 아날로그식 인상채득 후 이를 스캔하여 제작하는 방법이 사용되어 왔으나, 구강 내 디지털 스캔한 데이터를 이용하여 나사형 보철물을 제작하는 술식은 아직 활발히 이루어지지 않았다. 이에 본 증례보고에서는 6명의 환자를 대상으로 구강 내 스캐너인 iTero를 사용하여 디지털 스캔을 시행한 후 그중 다섯명은 맞춤형 지대주와 시멘트 합착 후 나사형 임플란트 보철물(screw-retained implant prosthesis after cementation, or SCRP)을 제작하였고, 임상적으로 기능과 심미적인 측면으로 만족할 만한 결과를 얻었기에 이를 보고하고자 한다. Fixed dental prostheses such as inlay, onlay, crown, and bridge fabricated by CAD/CAM technique combined with digital impressions is getting popular due to the recent rapid progress of digital impression taking system. For the scope of implant prosthesis, however, digital intra-oral scan hasn’t been actively utilized for the fabrication of superstructures. In this case report, 6 cases of titanium-milled custom abutment based on the iTero intra-oral scan data were introduced, five of them were restored with screw-type prosthesis after cementation (SCRP) and the clinical results were satisfactory on restoring the function and esthetics.

經驗生命表의 作成을 위한 補整模型의 硏究

권용만,장덕준,홍연웅 國立 昌原大學校 基礎科學硏究所 1993 基礎科學硏究所論文集 Vol.4 No.-

This paper presents a general law of mortality that is equal to a mixture of Greville's nonparametric graduation model(difference order 3) and Gompertz parametric survival function. Using crude death rates for constructing Korea experience life table (male, 1991), we know that Greville's model including a samll number of weighted moving average terms graduates well up to age 10, but there is no significant difference above age 60. And we propose a revised King-Hardy method for estimating parameters of Gompertz, and show that the revised method performs well in terms of bias and mean square error. Finally, a numerical example is given.

수종의 섬유보강재가 복합레진의 파절강도에 미치는 영향

박지만,조용범,홍찬의 대한치과보존학회 2000 Restorative Dentistry & Endodontics Vol.25 No.3

The effect of fiber reinforcing materials on the fracture strength of composite resin was evaluated. Each ten composite resin bars reinforced by glassfiber [Fiber-Splint MLR(polydentia SA. Switzerland)], polyethylene fiber [Ribbond R Ribbond Inc., U.S.A)] and polyaramid fiber [Kevlar R(DuPont U.S.A.)]were loaded under the 3-point compression technique. Another ten pure composite resin bars without reinforcement were used as a control group. Then mean fracture strength and standard deviation were calculated and a ANOVA and Scheffe test were used in statistics. The results were as follows: 1.Kevlar group showed the highest fracture strength as 175.5MPa (p〈0.05). Fiber-splint ML group showed the lowest fracture strength as 112.7MPa. 2.The mean value of fracture strength in Ribbond group was 136.4MPa, and that of ulcerated control group was 143.6MPa. No difference was found between the two groups. 3.Ribbond and Kevlar reinforcement groups showed a catastrophic failure, where complete separation of pieces occurs to a unaspirated fracture pattern. The use of Kevlar reinforcement fibers with composite resin showed significant increase in the average load failure and the presence of the fibers did prevent the catastrophic crack propagation present in the unreinforced samples. The use of Ribbond reinforcement fibers with composite resin showed no significant increase in the average load failure. However, the presence of the fibers did prevent the catastrophic crack propagation. Because high strength of glassfiber are rapidly degraded on exposure to moisture and humidity. The use of Fiber-splint ML reinforcement fibers with composite resin showed significant decrease in the average load failure and displayed catastrophic fractures.

PSM을 적용한 반도체 산업에서의 재해예방 사례연구

윤용구,홍성만,손제성,박범 대한설비관리학회 2003 대한설비관리학회지 Vol.8 No.2

It is a semiconductor product industry womed out enormous of loss that happened to accident for during in production exist to leakage, fire, explosion, using to variable chemical material of application, treatment, storage. This is presented to that improvement example for study, research, investigation of PSM in semiconductor industry and so processed to follow as theory consideration for PSM in semiconductor industry. This study did the object of research that S company is point to appointment and so give a account of S company PSM contents, searching to conclusion of PSM and consideration of problem and injury prevention for PSM expansion. From now on, PSM of semiconductor-injury is bring up improvement of problem, large-scale -company of semiconductor.

反芻家畜의 飼料로 利用하기 위한 鷄糞사일리지의 衛生學的 硏究

鄭永健,李萬佶,尹水弘 嶺南大學校 環境問題硏究所 1982 環境硏究 Vol.2 No.1

This study was carried out to evaluate the hygienical value of silage made from cage layer excreta, rice straw and wheat bran. Cage layer excreta and rice straw were mixed in the ratio of 100:10 and 70:30 with 0 and 10% levels of wheat bran in each treatment. Ensilage was made in the small polyethylene vinyl bags containing 1.5kg of mixtures. Results obtained are summarized as follows: 1) Total bacteria counts of the initial mixtures were quite high but reduced rapidly after 3 days fermentation, especially on the mixtures of 63% layer excreta: 27% rice straw; 10% wheat bran. 2) Two salmonella groups (B and D) were isolated from the silage of 3 days fermentation and of 30 day-fermentation, respectively. However, no pathogenicity exhibited when in-jected two isolated into the abdominal cavity of each mouse. 3) Fairly high contents of lead were analyzed from a layer excreta, but diluted with the mixing of rice straw and wheat bran. 4) These results suggest that the mixture of 63% layer excreta: 27% rice straw: 10% wheat bran is desirable, considering hygienic aspects.

경막외 스테로이드 주입에 의한 경부 신경근증의 치료

신근만(Keun Man Shin),홍순용(Soon Yong Hong),최영 대한통증학회 1991 The Korean Journal of Pain Vol.4 No.2

N/A Cervical epidural steroid injection, although not as familiar to many anesthesiologists, can be useful in the management of patients with acute and chronic neck, shoulder and arm pain. My clinic personally contacted and interviewed thirty patients with cervical radiculopathy who re- ceived cervical epidural steroid injection. Twenty seven percent of the patients had a excellent response(greater than 75% improvement) and fifty percent of the patients had a good response (greater than 50% improvement) to an injection of steroid into the cervical epidural space. We have conciuded that cervical epidural steroid injection was very effective in the management of cervical radiculopathy and represented a possible alternative to surgery. Many anesthesiologists should add to their armamentarium the use of such techniques in the man- agement of cervical radiculopathy.