산뽕나무(Morus bombycis)와 산딸기나무(Rubus crataegifolius) 추출물의 항균효과에 관한 연구

한영숙,이아영,성기옥,박주연 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To search antimicrobial compounds, methanol extracts of Morus bombycis and Rubus crataegifolius were fractionated by 4 solvents according to polarity. Antimicrobial effects against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes were measured by paper disc method and minimum inhibitory concentration(MIC)s were determined. The butanol fraction of Morus bombycis showed an antimicrobial effect against all test microorganisms. Especially, the butanol fraction was strong effective against L. monocytogens. All fractions of Rubus crataegifolius had an antimicrobial effect against S. aureus only. In the fractions of Morus bombycis, the chloroform fraction against S. aureus, the hexane fraction against S. typhimurium and L. monocytogenes showed an MIC of 39.063㎍/ml. The MIC of the methanol extract of Rubus crataegifolius was determined as 19.531㎍/ml MEex against L. monocytogenes.

증편 구조에 미치는 쌀 단백질의 영향

이해은,이아영,박주연,우경자,한영숙 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.4

The aim of this study was to examine the effect of rice protein on the network structure of the Jeung-Pyun. The major component of Jeung-Pyun rice protein was extracted, the change of rice protein during the Jeung-Pyun fermentation was assessed,and the effect on the viscosity and volume of adding protease to Jeung-Pyun was investigated. In addition, the result of adding protein to rice starch on the viscosity and volume of Jeung-Pyun was that the rice protein mediated the volume and expansion ability. The results were as follows. In rice and dough of Jeung-Pyun, the SDS soluble protein content was higher than that of wheat flour and no change was detected in the amount of extracted protein with the fermentation time. However, in the FPLC pattern, low molecular weight peaks were decreased with the fermentation time, which indicates the increase in the ratio of high molecular weight substances. In contrast, the addition of protease substantially decreased,the viscosity and volume of Jeung-Pyun, whereas the viscosity and volume were increasedby adding protein to rice starch in order to reconstitute Jeung-Pyun. This suggested that rice protein in Jeung-Pyun had a mediating effect on both the volume and the formation of the texture.

QLF를 이용한 항우식 제품의 인공우식 재광화 효과에 대한 연구

송주현,김영재,김정욱,장기택,김종철,한세현,이상훈 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.2

본 연구는 항우식 제품인 저농도의 불소 양치액(500 ppm NaF)과 Casein phosphopeptide-amorphous calcium phosphate(CPP-ACP)를 포함하는 치아 연고,그리고 이 두 제품을 같이 사용했을 경우,인공 우식 병소의 재광화 효과를 알아보고자 하였다. 제3대구치 협면에 인공우식을 유발하였고,시편을 16개씩 4군으로 나누어,28일 동안 매일 2회 다음과 같은 항우식 제품을 시편에 처리하였고,pH 순환 시스템을 적용하였다. 1군: 대조군(무처치) 2군: Tooth Mousse^(ⓡ) (GC Co. Japan) 3군: 치카치카^(ⓡ)(삼일제약. 한국) 4군: 치카치카^(ⓡ)+ Tooth Mousse^(ⓡ) 무기질 소실량(ΔQ)의 장기적인 변화를 Quantitative light-induced fluorescence (QLF)를 이용하여 관찰하여 다음과 같은결과를 얻었다. 1. ΔQ는 1군에서는 28일동안 유의성 있는 차이를 보이지 않았고,2군과 3군에서는 처치전에 비해 14일부터 유의성 있는 증가를 보였으며,4군에서는 7일부터 유의성 있는 증가를 보였다. 2. ΔQ는 1군 < 2군,3군 < 4군 순이었다. 3. ΔQ는 1군과 비교시 2군은 7일,3군과 4군은 3일부터 유의성 있는 증가를 보였고,2군은 3군과 전 기간 동안 유의차가 없었다. 4군은 2군과 3군에 비해 28일에 유의성 있는 증가를 보였다. 4. 모든 군에서 재광화 속도는 점차 감소하였다. The purpose of this in vitro study was to compare the remineralization effect of commercially available anticariogenic products, exactly low level fluoride mouthrinse(500 ppm NaF), tooth cream with Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride mouthhnse plus tooth cream on artificial caries lesion. Artificial caries lesion was induced at the buccal surface of permanent third molar and the specimens were then divided (16 specimens/group) into four group. Twice a day during 28 days specimens of each group were treated with a commercially anticari.ogen.ic product as follows and applied to the pH cycling system. Group 1: control group (No treatment) Group 2: Tooth Mousse^(ⓡ) (GC Co. Japan) Group 3: chikachika^(ⓡ) (Samil Co. Korea) Group 4: chikachika^(ⓡ) + Tooth Mousse^(ⓡ) The long-term change of mineral loss(ΔQ) was evaluated by quantitative 1ight-induced fluorescence(QLF) and the following results were obtained: 1. ΔQ of Group 1 was not noticed statistically significant during 28 days comparing that prior to treatment. There was a statistically significant increase in ΔQ of Group 2 and 3 since 14 days. So was in ΔQ of Group 4 since 7 days. 2. ΔQ was increased as follows: Group 1< Group 2, 3< Group 4. 3. Comparing with Group 1, Group 2 was a statistically significant increase since 7 days and Group 3 and 4 were since 3 days. Comparing Group 2 with 3, there was not noticed statistically significant during whole duration. Group 4 was significantly higher than Group 2 and 3 after 28 days. 4. All groups demonstrated a decrease in the rate of remineraUzation as time goes on.

뮤코다당증(Mucopolysaccharidosis)환아의 치은 증식

송주현,장철호,김영재,한세현,이상훈 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

뮤코다당증(Mucopolysaccharidosis, MPS)은 gIycosaminoglycans (GAGs)의 분해에 필요한 라이소좀 효소(lysomal enzymes)의 결함으로 GAGs의 대사 산물이 세포의 라이소좀내에 축적되어 점차 세포와 조직, 기관의 기능 이상을 초래하고 신체 질환과 정신 발달 지연을 가져오는 질환이다. 본 증례는 심각한 치은 증식을 가진 뮤코다낭증 환아를 전신 마취 하에 치은 절제술을 시행한 치험례이다. 소아치과 의사는 뮤코다당증 환아의 구강내 증상과 치과 치료시 주의 사항에 대해 숙지해야 하고, 내과의와 연계하여 환아의 전신 상태 변화에 민감하게 대처하여 치과 치료를 시행해야 한다. Mucopolysaccharidosis (MPS) is a disorder of storage in which there is excessive accumulation of glycosaminoglycans(GAGs) from lysosomal enzyme defect. Lysosomal accumulation of GAGs eventually results in cell, tissue and organ dysfunction. This patient may manifest mental retardation and physical disorders. This clinical report presents a girl with MPS having severe gingival hyperplasia. Gingivectomy was performed under general anesthesia. The pediatric dentist must be aware of oral manifestations present in the MPS. The approach to dental management will require teamwork between the dentist and the patient' s physician.

탄산음료 유발 법랑질 침식에 대한 항우식 제품의 예방 효과

송주현,김영재,김정욱,장기택,김종철,한세현,이상훈 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

본 연구는 항우식 제품인 Casein phosphopeptide-amorphous calcium phosphate(CPP-ACP)를 포함하는 치아크림, 불소 바니쉬, 저농도 불소 양치액을 치아에 도포하는 것이 단시간 탄산음료에 의한 법랑질의 침식을 예방할 수 있는지 살펴보기 위해 계획되었다. 법랑질 시편에 다음과 같은 항우식 제품을 도포하였고, 인공타액에 24시간 보관한 후, 콜라에 1분 증류수에 1분씩 5회 번갈아 처리하였다. 1군: 대조군(무처치) 2군: CPP-ACP 치아크림군 3군: 불소 바니쉬군(1,000 ppm F) 4군: 저농도 불소 양치액군(227 ppm F) 5군: 불소 바니쉬 + CPP-ACP 치아크림군 6군: 저농도 불소 양치액 + CPP-ACP 치아크림군 미세경도와 침식깊이를 측정하였고, Quantitative light-induced fluorescence(QLF)를 이용하여 안정된 형광 격자에 대한 부피 형광 변화인 ΔQ를 측정하여 무기질 감소량을 평가하였다. 6일 동안 실험을 반복하여 다음과 같은 결과를 얻었다. 1. 미세경도는 1군≤2군≤4군<6군<3군≒5군 순이었다. 2. 평균 침식깊이는 5군≒3군<6군<4군≒2군≒1군 순이었다. 3. ΔQ는 1군≒2군≤4군≤6군≤3군≒5군 순이었다. ΔQ의 감소율은 1군과 2군, 4군과 6군, 3군과 5군이 각각 유사하였다. 4. ΔQ는 미세경도와 강한 양의 상관관계를 나타냈고(r=0.96, p<0.05), 침식깊이와는 강한 음의 상관관계를 보였다(r=-0.96, p<0.05). The aim of this study was to evaluate the preventive effect of commercially available anticariogenic products, specifically, the tooth cream containing Casein phosphopeptide-amorphous calcium phosphate(CPP-ACP), fluoride varnish and low-level fluoride mouthrinse on enamel erosion induced by carbonated beverage in a short period of time. Enamel specimens were treated as follows and were then kept in artificial saliva for 24 hours followed by further processing by alternately soaking them in Cola beverage and in distilled water for 1 minute each five times. Group 1: control group (no treatment) Group 2: tooth cream with CPP-ACP Group 3: fluoride varnish (1,000 ppm F) Group 4: low-level fluoride mouthrinse (227 ppm F) Group 5: fluoride varnish + tooth cream with CPP-ACP Group 6: low-level fluoride mouthrinse + tooth cream with CPP-ACP Microhardness and erosion depth were measured and the mineral loss of each specimen was evaluated by measuring the volumetric fluorescence change(ΔQ) against the stable fluorescent grid using quantitative lightinduced fluorescence(QLF). The experiment lasted for 6 days repeated each day. The results were as follows: 1. The microhardness was increased as follows: Group 1≤2≤4<6<3≒5. 2. The mean erosion depth was increased as follows: Group 5≒3<6<4≒2≒1. 3. The ΔQ was increased as follows: Group 1≒2≤4≤6≤3≒5. The decrement of ΔQ was similar between group 1 and 2, group 4 and 6 and group 3 and 5. 4. The ΔQ showed positive correlation with microhardness (r=0.96, p<0.05), while it was negatively correlated to erosion depth (r=-0.96, p<0.05).

4종의 식품 부패 미생물에 대한 국내산 자생 식물 열매 추출물의 항균성 탐색

권민경,이해은,박주연,한영숙 동아시아식생활학회 2003 동아시아식생활학회지 Vol.13 No.5

To select new useful domestic plants with antimicrobial activities, thirty five samples of berry plant were distributed from Plant Diversity Research Center in Korea Research Institute of Bioscience and Biotechnolog. Their extracts with methanol were tested against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Listeria monocytogenes by paper disc method. The methanol extracts from Viburnum dilatatum, Viburnum erosum, Aralia continentalis, Camellia japonica, Acer trumcatam, Arasaema takesimense and Rhysocarpus intermedius were effective against S. aureus and the results were as follows: Viburnum erosum 6.5 ㎜, Viburnum dilatatum and Aralia continentalis 7.0 ㎜, Rhysocarpus intermedius 8.0 ㎜, Acer to mcatum 9.0 ㎜, Camellia japonica and Arisaema takesimense 9.5 ㎜ And only the methanol extract from Camellia japonica was effective against L. monocytogenes, 7.0 ㎜. Antimicrobial activities of E. coli and S. typhymurium were not detected. The minimum inhibitory concentration(NIC) of berry of Camellia japonica was examined as 1,250 ㎍/mL against S. aureus and as 1,250 ~ 2,500 ㎍/mL against L. monocytogenes.

A Novel Digital Pulse-Shape Analysis for High-Resolution Position-Sensitive Gamma-Ray Spectroscopy

Ju Hahn Lee,Hyo Soon Jung,Hwa Youn Cho,Young Kwan Kwon,Chun Sik Lee IEEE 2010 IEEE transactions on nuclear science Vol.57 No.5

<P>A novel digital pulse-shape analysis (DPSA) was performed for high-resolution position-sensitive gamma-ray spectroscopy with coaxial-type high-purity germanium (HpGe) detectors. The DPSA consisted of two stages that determined the pulse height, the interaction point and the start time by using the chi-square fitting the digitally recorded pulse shapes with calculated shapes. As the DPSA was applied to gamma-ray spectroscopy using HpGe coaxial detectors, we obtained the energy spectra, the time-difference spectra and the interaction depth profiles for the gamma rays emitted from <SUP>133</SUP>Ba and <SUP>22</SUP>Na standard sources. The efficiency of the spectroscopic analysis using the DPSA was compared with that using a conventional analog signal process (CASP). Furthermore, we determined the half-lifetime of the first excited state in <SUP>133</SUP>Cs by using the DPSA.</P>

Cultural Adaptation of a Compliance Questionnaire for Patients with Rheumatoid Arthritis to a Korean Version

( Ju Yeun Lee ),( So Young Lee ),( Hyeon Joo Hahn ),( In Ja Son ),( Suh Gyung Hahn ),( Eun Bong Lee ) 대한내과학회 2011 The Korean Journal of Internal Medicine Vol.26 No.1

Background/Aims: The Compliance Questionnaire-Rheumatology (CQR) is a validated scale to evaluate patient compliance for anti-rheumatic medications. We developed a Korean version of the CQR (KCQR) and confirmed its reliability and validity. Methods: We prepared the KCQR by translating and back-translating the original CQR with modifications to adapt it to Korean culture. Fifty Korean patients with rheumatoid arthritis (RA) were enrolled in this study. The test-retest reliability of the KCQR was evaluated at a 2-week interval using the intraclass correlation coefficient (ICC). The validity of the KCQR was assessed by identifying associations between KCQR scores and patient compliance, measured using pharmacy refill data. Results: The reliability of the KCQR was adequate, with an ICC of 0.71 for test-retest reliability. With respect to validity, the summed score of the weighted KCQR showed a significant correlation with pharmacy refill data (r2 = 0.57) on multiple regression analysis. Conclusions: Our results indicate that the KCQR is a reliable, valid instrument to evaluate compliance of Korean patients for RA medications. (Korean J Intern Med 2011;26:28-33)

만성골수성백혈병에서의 동종골수이식 : 국내 경험의 후향적 분석 Retrospective Analysis of Korean Experience

이규형,이제환,이정신,김상희,김우건,김춘추,이종욱,김병국,박선양,이홍기,윤성수,김효철,남동기,한지숙,민유홍,현명수,황태주,정익주,박영석,이정애 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.2

연구방법: 국내에서 시행된 만성골수백혈병의 치료를 위한 가족간 동종골수이식의 임상적 특징을 후향적으로 분석하였다. 9개의 병원에서 1986년 9월부터 1997년 6월까지 시행한 137예의 가족간 동종골수이식을 보고하였다. 결과: 전체 환자 중 62%인 85예가 남자였고 연령은 15세에서 50세까지 분포하였다(중앙치 30세). 95예(71%)의 환자는 이식 당시 만성골수성백혈병 만성기의 상태였고 38예(28%)는 가속기 또는 모세포성발증기의 상태였다. 70예(51%)의 환자는 이식전처치로 busulfan+cyclophosphamide (BuCy) 치료를 받았고 42예(31%)의 환자는 cyclophosphamide+전신방사선 조사(Cy-TBI)를 받았다. 환자에게 이식된 단핵구의 수는 0.06-9.5×10^(8)/kg(중앙치 1.2×10^*/kg)였다. 1 예를 제외한 모든 환자에서 이식 후 8-100일(중앙치 17일)에 중성구 500/㎕ 이상의 착상이 관찰되었다. 급성 및 만성이식편대숙주질환은 각각 46%와 43%의 환자에서 관찰되었다. 중앙추적기간 20개월(범위 3-135개월) 경과 후 137예의 환자 중 52예의 환자가 사망하였다. 사망 원인은 감염(27%), 백혈병의 지속 또는 재발(23%), 이식편대숙주질환(21%), 치료독성(15%), 그리고 이식편거부(8%) 등이었다. 환자들의 event-free survival에 영향을 주는 인자로는 이식전 병의 상태(p<0.001)와 환자의 혈청 HBsAg 양성여부(p=0.012)가 중요하였다. Overall survival에 영향을 주는 인자도 이식전 병의 상태와 환자 혈청 HBsAg 양성여부로 나타났다. 결론: 본 연구에서 국내 만성골수성백혈병에 대한 동종골수이식술은 그 시행 예가 급격히 증가하고 있음을 보여주었다. 환자들의 임상 특성과 생존 성적은 외국의 성적과 유사하였다. B형 간염 감염환자의 경우 예후가 유의하게 불량하였고, 이는 추후의 임상연구에서 확인이 필요한 소견으로 사료된다. Background and Methods: Clinical features of allogeneic bone marrow transplantation (BMT) performed for patients with chronic myelocytic leukemia (CML) in Korea were analyzed retrospectively. Nine hospitals reported a total of 137 cases of allogeneic BMT performed between September, 1986 and June, 1997. Results: There were 85 male (62%) and 52 female patients with median age of 30 years (range 15-50). Ninety five patients (71%) were in chronic phase and 38 patients (28 %) were in accelerated/blastic phase of the disease. Seventy patients (51%) received busulfan-cyclophosphamide (BuCy) regimen for preparation and 42 patients (31%) received cyclophosphamide-total body irradiation (Cy-TBI) regimen. The median mononuclear cell dose given to patients was 1.2×10^(8)/kg (range, 0.06-9.5×10^(8)/kg). All but one patient achieved absolute neutrophil count of 500/μl after median 17 days after BMT (range, 8-100 days). Acute and chronic graft-versus-host disease (GVHD) were reported in 61/133 (46%) and in 57/134 (43%) patients, respectively. The median follow-up time of survivors was 20 months (range, 3-135 months). There were 52 deaths (38%). The causes of death were infection (14 patients, 27%), recurrent/persistent disease (12 patients, 23%), GVHD (11 patients, 21%), regimen related toxicity (8 patients, 15%), graft rejection/failure (4 patients, 8%), and unknown (3 patients, 6%). The factors influencing event free survival of the patients were disease status before BMT (p<0.001, by log-rank test) and serum hepatitis B surface antigen status (p=0.012). Longer duration of disease before BMT showed poorer outcome but the difference did not reach statistical significance. The factors affecting overall survival of the patients were disease status before BMT (p=0.009) and hepatitis B surface antigen status (p=0.036). The donor hepatitis B antigen status did not influence event free survival or overall survival of the patients. Conclusion: Clinical features and survival patterns of patients with CML treated with allogeneic BMT in Korea were similar to historical data from other countries. Further data analyses are warranted for the role of hepatitis B virus infection influencing the outcome of allogeneic BMT for CML.

Monte Carlo Study on the Imaging Resolution and Sensitivity of a Compton Camera

Ju Hahn Lee,이춘식 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.1

We have used a Monte Carlo simulation to investigate the influence of the detector parameters (material, thickness, and geometrical arrangement, etc.) on the imaging resolution and sensitivity of a Compton camera. By comparing the two gamma-ray interaction characteristics in the material, the fraction of Compton-scattering events with respect to photo-absorption events, and the energy spread due to the intrinsic resolution and the Doppler broadening for three kinds of semiconductor materials, Si, Ge and CdTe, we found that silicon and germanium are the most suitable materials for the scatterer and the absorber, respectively, of a Compton camera and that the effective thickness of silicon is about 2.5 cm. Through a Monte Carlo simulation that was performed for a Compton camera consisting of a silicon detector and a germanium detector, we could also examine the dependence of the imaging resolution and the imaging sensitivity of the Compton camera on the geometrical parameters, including the source-to-scatterer distance, the scatterer-to-absorber distance, and the interaction position uncertainty of the detectors. The simple backprojection method was used to reconstruct the images from the simulated data.