관상 동맥 질환에서 아포 E 지단백 유전자 다형성과 혈청 지질치와의 관계

곽선영,김성구,정호석,이유경,이광희,김철현,최태명,현민수,권영주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background and aims: The Apolipoprotein E is a ligand of both the protein component LDL receptor as well as the apo E LDL-Receptor related protein (LRP). It modulates the receptor binding of lipoproteins, with the apolipoprotein E found on cell surfaces as its component, thus serving an important role in the lipid metabolism by carrying out the intracellular transport of cholesterol in lipoproteins. The gene for apolipoprotein E is the product of three common genotypes as well as many more rare alleles. The common genotypes are ε2, ε3, and ε4, and are expressed in the three phenotype isoforms of E2, E3, and E4. In the event that E4 is the main component, a rise in the cholesterol level, as the result of down-regulation of the LDL receptor, is observed. Therefore, those samples with E4 genotypes are known to be in much higher risk of coronary artery disease than those with ε3/ε3, while those with ε2 are in low risk (with the exception of hypertiglyceremai Ⅲ). The aim of this study is to analyze in patients with ischemic heart disease the role of aplipoprotein E alleles in order to seek its correlation with coronary artery disease, as well as to seek whether the polymorphism of apo E produces any differences in the severity of coronary artery disease according to plasma lipid levels. Methods: The subjects for study were 273 patients admitted to the Internal Cardiology Division of the Soonchunhyang University Hospital form December 1998 to February 1999. The subjects were divided into the two groups of which one was ischemic heart disease (IHD) experiment group totaling 105 (avg.60.1 years of age, male/female ratio = 69/36) and the control group totaling 168 (avg. 59.7 years of age, male/female = 73:95). The coronary angiogram was given to 127 subjects, and of this total, 94 have developed significant stenosis in the coronary artery. The stages of the analyzing of the apo E phenotype was first, the separation of DNA from the blood samples, subjecting it to the PCR from with 228 base pairs of expanded products were obtained. The band was determined by means of the reverse hybridization principle on the nitrocellulose strip. Results: From the 105 patients the distributions of apo E phenotypes were as follows: ε3/2(5.7%), ε4/2(1.9%), ε3/3(70%), ε4/3(20%), ε4/4(1.9%). The relative frequencies of each allele are as follow: ε2 (0.038), ε3 (0.833), ε4 (0.128). The results show as follows: ⅰ) The IHD experiment group to have a higher occurrence of ε4/3 phenotypes as well as ε4 alleles than the control group. ⅱ) Both the control group and IHD group showed the largest distribution of ε3/3 for phenotypes, and ε4 for alleles. ⅲ) The IHD group showed less ε2/3 phenotypes as well as significantly less allele frequency of ε3 in comparison to the control group. ⅳ) the IHD group showed a much lower level of HDL in comparison to the control group, while the LDL was significantly higher; samples including the apo ε2 showed a significantly higher level of HDL than those without. Among the control group, samples including apo ε2 showed a significantly higher level of TG (triglyceride) than samples without. No significant difference was found between the experiment apo ε4 sample and the control plasma lipid sample. ⅴ) No significant correlation was found between an apo E polynorphism and the number of involved arteries of a coronary angiogram. Conclusion: Between the experiment IHD group and control group were found differences in the frequency of alleles. The polymorphism of apo E alleles may contribute as a risk factor to the development of heart disease by involving itself in the metabolism and modulation of plasma lipids.

Lutembacher 증후군 환자 증례 보고

김성범,서정주,곽철훈,김상민,이보라,민선경,황은구,김용인,조욱현,최석구 인제대학교 2008 仁濟醫學 Vol.29 No.-

Lutembacher's syndrome is rare combination of mitral stenosis (MS) and atrial septal defect (ASD). The hemodynamic interplay between the MS and ASD leads to wide variation of clinical presentation. Here we describe a 43-year-old female with Lutembacher's syndrome and tricuspid regurgitation with pulmonary hypertension, who underwent direct closure of ASD and tricuspid valvuloplasty successfully. We also reviewed other literatures in an effort to increase awareness of this condition.

CD4+/CD8+ Ratio and Growth Differentiation Factor 8 Levels in Peripheral Blood of Large Canine Males Are Useful Parameters to Build an Age Prediction Model

Lee Han-Jun,Hong Seok-Jin,Kim Seung-Soo,Kwon Young-Yon,Choi Bong-Hwan,Choi Kyung-Mi,Choi Kyung-Mi,Lee Myung-Jin,Hwang Sun-Young,Park Kyoungwan,Joo Younghun,Song Hwayoung,Lee Cheol-Koo 대한남성과학회 2022 The World Journal of Men's Health Vol.40 No.2

Purpose:Purpose: To build an age prediction model, we measured CD4+ and CD8+ cells, and humoral components in canine periph-eral blood. Materials and Methods:Materials and Methods: Large Belgian Malinois (BGM) and German Shepherd Dog (GSD) breeds (n=27), aged from 1 to 12 years, were used for this study. Peripheral bloods were obtained by venepuncture, then plasma and peripheral blood mono-nuclear cells (PBMCs) were separated immediately. Six myokines, including interleukin (IL)-6, IL-8, IL-15, leukemia inhibitory factor (LIF), growth differentiation factor 8 (GDF8), and GDF11 were measured from plasma and CD4+/CD8+ T-lymphocytes ratio were measured from PBMC. These parameters were then tested with age prediction models to find the best fit model. Results:Results: we found that the T-lymphocyte ratio (CD4+/CD8+) was significantly correlated with age (r=0.46, p=0.016). Among the six myokines, only GDF8 showed a significant correlation with age (r=0.52, p=0.005). Interestingly, these two markers showed better correlations in male dogs than females, and BGM breed than GSD. Using these two age biomarkers, we could obtain the best fit in a quadratic linear mixed model (r=0.77, p=3×10-6). Conclusions:Conclusions: Age prediction is a challenging task because of complication with biological age. Our quadratic linear mixed model using CD4+/CD8+ ratio and GDF8 level showed a meaningful age prediction.

위내시경으로 진단된 원발성 위 유전분증 1예

이석호,손희정,이규택,김재준,강인구,백승운,이종균,이준혁,고광철,이풍렬,오성윤,이종철,최규완,오영륜 대한소화기내시경학회 1998 Clinical Endoscopy Vol.18 No.4

Amyloidosis is characterized by deposition of amyloid, which is resistant to proteolysis & phagocytosis, in intercellular spaces & vascular walls. The amyloid deposition provokes dysfunction of an accumulated organ & displays variable clinical symptoms depending upon the involved organ. A diagnosis is rendered through a biopsy of the affected organ, followed by staining using congo red which reveals an apple greenish refractile birefringence via polarizing microscopy. Using an electro-microscopy specific filaments can be found. Amyloidosis is classified into primary amyloidosis, composed of light chain filaments (AL) and secondary amyloidosis, comprised of A protain (AA). The AL type of amyloidosis shows deposition of amyloid in muscularis mucosa & muscularis externa. Priunary amyloidosis on the other hand, is relatively rare. We experienced primary stornach amyloidosis (AL), which was mistaken for stomch cancer.

간암 환자의 술전 검사로서 CTAP와 CTHA 의 유용성

이석호,이규택,김재준,강인구,김승훈,백승운,도영수,이종균,이준혁,고광철,이풍렬,최규완,오성윤,이종철 대한간학회 1997 Clinical and Molecular Hepatology(대한간학회지) Vol.3 No.4

Background / Aims : Combined computed tomography during arterial portography(CTAP) and computed tomography during hepatic arteriography(CTHA) are more sensitive methods compared to conventional spiral CT in detection small hepatocellular carcinoma. However, there are few data available on the influence of combined CTAP and CTHA on decision making for treatment of hepatocellular carcinoma. We aimed to evaluate the usefulness of CTAP and CTHA as a preoperative study of hepatocellular carcinoma for surgical treatment. Methods : Eighty -two patients with histologically proven hepatocellular carcinoma who were considered to be surgical candidates on spiral CT and angigraphy were included. CTAP and CTHA were performed as the routine protocol. The findings of CTAP and CTHA were analyzed and compared to conventional spiral CT and angiography. Results : 1)105 nodules were found on spiral CT plus angiography , whereas, 138 nodules found on combined CTAP and CTHA : 33 additional nodules were found in 23 cases(28%). 2) The treatment plan changed from surgery to nonsurgical treatment in 10(12%) cases : 9 cases(10%) with bilobar involvement, and 1 case(1%) with more than three nodules. 3) The larger the tumor nodules on the spiral CT, the more additional tumor nodules were found on CTAP and CTHA : combined CTAP and CTHA were able to find more nodules in 21% of tumor ≤3cm, 27% of tumor with 3- 10cm, and 71% of tumor > 10 am on spiral CT (p=0.03). Conclusion : Compared to spiral CT plus angiography, CTAP and CTHA may be useful in avoiding unnecessary operation of hepatocellular carcinoma, especially in large tumors.

MOLECULAR EPIDEMIOLOGY OF AVIAN POXVIRUS IN THE ORIENTAL TURTLE DOVE (<i>STREPTOPELIA ORIENTALIS</i>) AND THE BITING MIDGE (<i>CULICOIDES ARAKAWAE</i>) IN THE REPUBLIC OF KOREA

Lee, Hae Rim,Koo, Bon-Sang,Kim, Jong-Taek,Kim, Heung-Chul,Kim, Myung-Soon,Klein, Terry A.,Shin, Man-Seok,Lee, Sanghun,Jeon, Eun-Ok,Min, Kyung-Cheol,Lee, Seung Baek,Bae, Yeonji,Mo, In-Pil Wildlife Disease Association 2017 JOURNAL OF WILDLIFE DISEASES Vol.53 No.4

<P>A total of 600 wild birds were analyzed for the causes of mortality in the Republic of Korea (ROK) from 2011 to 2013. Avian poxvirus (APV) infections were identified as the primary cause of mortality in 39% (29/74) Oriental Turtle Doves (Streptopelia orientalis). At necropsy, all 29 S. orientalis birds, of which, 76% (22/29) were juveniles, had severe diphtheritic lesions in their oral and nasal cavities and on their eyelids, which were the lesions of APV that resulted in mortality. We detected APV infection by chorioallantoic membrane inoculation and molecular study of the partial region of the P4b gene. All isolates belonged to the same APV strain and were identical to strains isolated from several different pigeon species in South Africa. Phylogenetically, the APV strain identified in S. orientalis belonged to subclade A2, which includes isolates from several species of pigeons from different parts of the world, including the United Kingdom, Germany, India, Egypt, Hawaii, Georgia, Hungary, South Africa, Tanzania, and the ROK. This identity indicated that this diphtheritic APV strain may be a potential pathogen of other pigeon species in the ROK and neighboring countries throughout the range of S. orientalis. However, reticuloendotheliosis virus insertion into the APV genome was not detected by PCR in any of the 29 APV infections. An identical strain of APV observed in S. orientalis was also detected in Culicoides arakawae (biting midge), with annual peak populations corresponding to the presence of APV in S. orientalis. Culicoides arakawae may be a primary vector of APV in S. orientalis. Active surveillance of APVs in wild birds and C. arakawae is needed to better understand the epidemiology of APVs, host-vector relationships, and its ecological effects on S. orientalis in the ROK.</P>

내시경적 조직검사로 진단된 위 분선충증 1 예

이석호,이화영,이규택,강인구,최규완,백승운,이종균,이준혁,고광철,이종철,오영륜,현재근,이풍렬,김재준,채종일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.2

Strongyloidiasis is a parasitic disease caused by Strongyloides stercoralis which exists in two forms : the free living and parasitic forms. It exists in warm, moist climate in areas where there is frequent fecal contamination of the soil. After cutaneous invasion by the filariform larvae, petechial hemorrage, pruritus, papular rashes, edema, and urticaria occur. Infection commonly occurs in the proximal intestine of the gastrointestinal (G-I) tract but may extend from the stomach to the anus. Once the worm is established in the small intestine, the physical findings may include epigastric tenderness to palpation. The mucosal biopsy is an inefficient way of making the diagnosis because the worm is found in the biopsy specimen in only 2% of patients. Gastric strongyloidiasis is rare. We experienced a case of gastric strongyloidiasis diagnosed by the endoscopic biopsy and serologic test for parasite specific IgG antibody by micro-ELISA.

기상요소에 따른 부산지역 계절별 교통사고 변화와 예측에 관한 연구

이동인,이문철,유철환,이상구,이철기 한국환경과학회 2000 한국환경과학회지 Vol.9 No.6

The traffic accidents in large cities such as Pusan metropolitan city have been increased every year due to increasing of vehicles numbers as well as the gravitation of the population. In addition to the carelessness of drivers, many meteorological factors have a great influence on the traffic accidents. Especially, the number of traffic accidents is governed by precipitation, visibility, humidity, cloud amounts and temperature, etc. In this study, we have analyzed various data of meteorological factors from 1992 to 1997 and determined the standardized values for contributing to each traffic accident. Using the relationship between meteorological factors(visibility, precipitation, relative humidity and cloud amounts) and the total automobile mishaps, an experimental prediction formula for their traffic accident rates was seasonally obtained at Pusan city in 1997. Therefore, these prediction formulas at each meteorological factor may be used to predict the seasonal traffic accident numbers and contributed to estimate the variation of its value according to the weather condition in Pusan city.

Tumor necrosis factor receptor 2 is required for ischemic preconditioning-mediated neuroprotection in the hippocampus following a subsequent longer transient cerebral ischemia

Lee, Jae-Chul,Park, Chan Woo,Shin, Myoung Cheol,Cho, Jun Hwi,Lee, Hyang-Ah,Kim, Young-Myeong,Park, Joon Ha,Ahn, Ji Hyeon,Cho, Jeong Hwi,Tae, Hyun-Jin,Hwang, In Koo,Lee, Tae-Kyeong,Won, Moo-Ho,Kang, Il Elsevier 2018 Neurochemistry International Vol.118 No.-

<P><B>Abstract</B></P> <P>Tumor Necrosis Factor-α (TNF-α) is a proinflammatory cytokine implicated in neuronal damage in response to cerebral ischemia. Ischemic preconditioning (IPC) provides neuroprotection against a subsequent severer or longer transient ischemia by ischemic tolerance. Here, we focused on the role of TNF-α in IPC-mediated neuroprotection against neuronal death following a subsequent longer transient cerebral ischemia (TCI). Gerbils used in this study were randomly assigned to eight groups; sham group, TCI operated group, IPC plus (+) sham group, IPC + TCI operated group, sham + etanercept (an inhibitor of TNF-a) group, TCI + etanercept group, IPC + sham + etanercept group, and IPC + TCI + etanercept group. IPC was induced by a 2-min sublethal transient ischemia, which was operated 1 day prior to a longer (5-min) TCI. A significant death of neurons was found in the stratum pyramidale (SP) in the CA1 area (CA1) of the hippocampus 5 days after TCI; however, IPC protected SP neurons from TCI. We found that TNF-α immunoreactivity was significantly increased in CA1 pyramidal neurons in the TCI and IPC + TCI groups compared to the sham group. TNF-R1 expression in CA1 pyramidal neurons of the TCI group was also increased 1 and 2 days after TCI; however, in the IPC + TCI group, TNF-R1 expression was significantly lower than that in the TCI group. On the other hand, we did not detect TNF-R2 immunoreactivity in CA1 pyramidal neurons 1 and 2 days after TCI; meanwhile, in the IPC + TCI group, TNF-R2 expression was significantly increased compared to TNF-R2 expression at 1 and 2 days after TCI. In addition, in this group, TNF-R2 was newly expressed in pericytes, which are important cells in the blood brain barrier, from 1 day after TCI. When we treated etanercept to the IPC + TCI group, IPC-induced neuroprotection was significantly weakened. In brief, this study indicates that IPC confers neuroprotection against TCI by TNF-α signaling through TNF-R2 and suggests that the enhancement of TNF-R2 expression by IPC may be a legitimate strategy for a therapeutic intervention of TCI.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ischemic preconditioning (IPC) protects CA1 pyramidal neurons from ischemic damage. </LI> <LI> IPC attenuates TNF-α and TNF-R1 expressions in ischemic CA1 pyramidal neurons. </LI> <LI> IPC increases TNF-R2 expressions in pericytes in CA1 area after ischemic insult. </LI> <LI> IPC-mediated neuroprotective effect is reversed by etanercept (an inhibitor of TNF-a) treatment. </LI> </UL> </P>

GaN-HEMT asymmetric three-way Doherty power amplifier using GPD

Koo, Hyungmo,Kang, Hyunuk,Lee, Wooseok,Lee, Hwiseob,Lee, Kang-Yoon,Hwang, Keum Cheol,Yang, Youngoo Institution of Electrical Engineers 2018 IET microwaves, antennas & propagation Vol.12 No.13