정상과 갑상선 종양조직에서 사람 IGF-I 유전자의 발현

김성운,장현하,박상미,김덕윤,우정택,양인명,김진우,김영설,김광원,고석환,홍성화,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

Many of the growth-promoting properties of growth hormone(GH) are mediated by insulin-like growth factor-I(IGF-I), a highly conserved circulating 70-amino acid peptide. Recent studies have shown that multiple mechanisms influence IGF-I gene expression, including transcription from two promoters, alternative RNA splicing, and variable polyadenylation. In thyroid tissue, thyroid stimulating hormone(TSH) and IGF-I are the most possible candidates for follicular cell proliferation and hypertrophy. Actually IGF-I had autocrine and paracrine effect for tissue growing. We prepared thyroid tumor tissue mRNAs using single step method for detecting IGF-I levels according to different tissues, i.e., thyroid adenoma or papillary thyroid carcinoma. We used Northern blot analysis for IGF-I mRNA and RNase protection assay (RPA) for IGF-I transcription start sites. For Northern blot, we used whole human IGF-I cDNA as a DNA probe and for RPA, we used IGF-I exon 1 containing noncoding promoter 1 as a riboprobe. We got good RNA bands from Northern blot analysis around 1 kb (IGF-IA) and 7.5 kb (IGF-IB) region. To clarify the amount of both IGF-IA and IB mRNAs, we measured autoradiographied signal of IGF-I mRNAs bands using densitometer. In IGF-IA signals, there's no change among liver and thyroid tissues, but in case of IGF-IB mRNA bands, the signal was markedly increased in thyroid carcinoma tissues than that of normal thyroid tissue (85% vs 14%). In the study of RPA, all thyroid tissues used the same transcription start sites as those of liver's. We concluded that that this different regulation of IGF-I mRNA was originated from tissue specificity. That meant some tissue specific transcription factor/s were related to tissue IGF-I expression.

『素問· 逆調論』에 대한 硏究

김화창,김종호,천상묵,서대선,김명수,금경수 한국전통의학연구소 2002 한국전통의학지 Vol.12 No.1

A Study on the Youkjoron(逆調論) of the So Moon(素問) one of the classic book of oriental medicine, was carried out analytically for the right comments. The principal ideas abtained summerized as follows: The unbalance between Eum(陰) and Yang(陽) The unbalance between Su(水) and Wha(火) The Yuk Ga(肉苛) which is caused by the unbalance of the Young Wi(營衛) The Asthma(喘息) which is caused by the unbalance of the Viscera(臟腑)

미혼 흡연여성들의 불안, 우울 및 자아존중감이 흡연양 및 니코틴 의존도에 미치는 영향

김옥수,김애정,김선화,백성희 성인간호학회 2001 성인간호학회지 Vol.13 No.4

Purpose: The purpose of the study was to investigate the effects of anxiety depression and self-esteem on the amount of smoking and nicotine dependency among woman smokers. Method: The convenience sample consisted of 121 unmarried woman smokers. The STAI, the CES-D Scale, the Self-Esteem scale and the FTQ were utilized to measure the level of anxiety, depression, self-esteem, and nicotine dependency. The amount of smoking was calculated based on the number of cigarette used. Results: Results indicated that woman smokers were depressed. Nicotine dependency was relatively low. Subjects had smoked a mean of 9.58 cigarettes per day. The level of depression influenced on smoking and nicotine dependency. Self-esteem influenced on the amount of smoking and anxiety influenced on nicotine dependency. Conclusion: The results of this study revealed that the amount of smoking of unmarried woman smokers was very high. Both the amount of smoking and nicotine dependency were affected by levels of depression. Education programs and campaigns are needed to prevent smoking of young adult women.

2-[(4-Cyanophenyl)amino]-3-chloro-1,4-naphthalenedione (NQ-Y15)의 돌연변이원성

김봉희,정기화,유충규,창동신,이기선,전선덕,소동수,채상호,문창규 德成女子大學校 藥學硏究所 2001 藥學論文誌 Vol.12 No.1

2-[(4-Cyanophenyl)amino]-3-chloro-1, 4-naphthalenedione (NQ-Y15) was asssayed for its genotoxic potential by using Salmonella typhimurium reversion assay and in vitro chromosome aberration test on Chinese hamster lung cells. In the Ames test, NQ-Y15 induced his+revertants of Salmonella typhimurium TA 98 and TA 1537, reaching levels twice the negative control values. But, NQ-Y15 induced only his+revertants of Salmonella typhimurium TA 1537 more than twice the control values under the condition with metabolic activation system. In the cytogenetic test on chinese hamster lung cells. NQ-Y15 showed significant chromosomal aberrations, but the incidence was significantly reduced in the presence of metabolic activation.

국내 분리 방선균의 항균활성 특성

김소연,박동진,권오성,임채영,김판경,이상화,김창진 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

제주도지역, 충청남도 서해안지역, 강원도 및 충청북도 자연 동굴지역, 그리고 충청북도 청원군 등지에서 채집한 205점의 토양시료로부터 분리된 총 1,322주의 방선균을 대상으로 Eschericia coli KCTC 1924, Salmonella typhimurium KCTC 1926, Staphylococcus aureus KCTC 1926, Staphylococcus aureus KCTC 1928, Candida albicans KCTC 1940등 5종의 시험균주에 대하여 항균활성을 조사하였다. 액체배양을 위해서는 G.S.S(glucose-soybean meal-starch) 배지와 fish meal 배지등 2종류의 배지를 사용하였으며 그 결과를 균분리원 지역별, 배지별, 시험균주별, 그리고 방선균 종류별로 비교하였다. It is well known that actinomycetes would be useful for screening of biologically active compounds. Not only to isolate diverse actinomycete strains but to ferment those strains effectively would be important. Seven hundred and forty six strains were isolated from Cheju province, 216 strains were from Chungnam province, 158 strains were from the natural caves at Chungbuk and Kangwon provinces and 202 strains were from Chungwon area at Chungbuk province. All of these 1,322 strains were fermented on a small scale using two different media and tested for their antimicrobial activities against four bacterial strains and one yeast strain. As the result, 12.3% of those isolates were active against Staphylococcus aureus KCTC 1916, 7.6% were Staphylococcus aureus KCTC 1928, 3.9% were Eschericia coli KCTC 1924, 3.0% were Canida albicans KCTC 1940, and 2.2% were Salmonella typhimurium KCTC 1926. About 40% of those isolates showed antimicrobial activities at both two media but the others showed at either one. According to the genus of isolated strains, Streptomyces and Micromonospora showed activities with higher frequencies than others.

치열 공간 부조화와 두개안면골격 형태의 상관성

김요숙,정애진,강경화,김상철 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.4

치열 공간 부조화에 영향을 미치는 골격 및 치성요소를 알아보기 위하여 하악 치열궁내 총생을 보이는 성인 45명과 공극을 보이는 성인 39명의 Ⅰ급 부정 교합 환자와 정상적인 배열을 갖는 성인 40명을 선정하여 측모 두부방사선사진과 모형을 분석하여 다음과 같은 결론을 얻었다. 1.공극군보다 총생군의 ANB각이 컸다. 2.공극군보다 총생군이 하악골 및 상악골의 전하방 경사가 커서 안모의 수직적 양상이 심했다. 3. 총생군보다 공극군이 전두개저와 하악체 길이가 컸다. 4. ANB 각, 전안면 고경과 하악지 길이가 공간 부조화 양의 예측에 기여하며, 다음과 같은 회귀방정식이 산출되었다. space discrepancy = 46.51 - 2.51ANB - 0.58AFH + 0.65RH (R² = 0.40) The purpose of this study was to investigate the relationship between the space discrepancy of the mandibular dentition and craniofacial morphology in adults with good Angle class I occlusion. Dental casts of normal group, crowded group and spaced group were selected on the basis of dental crowding and spacing. Subjects with excessive space to accomodate the lower teeth were classified as spaced group(39). Subjects with a space discrepancy of more than 4mm were classified as crowded group(45). Normal subjects had little or no dental crowding and spacing(40). Various skeletodental measurements in lateral cephalograms were evaluated and compared by ANOVA, Pearson correlation analysis and multiple stepwise regression analysis. The results were as follows : 1. ANB angle was larger in crowded group than in spaced group. 2. Maxilla and mandible in crowded group were inclined more downward and forward than in spaced group, so crowded group was found to have vertical tendency. 3. Anterior cranial base length and mandibular body length were longer in spaced group than in crowded group. 4. According to the multiple stepwise regression analysis with space discrepancy as dependent variable, 40% of variance of space discrepancy could be explained by ANB angle, anterior facial height and ramus height. Multiple regression equation was as follows Space discrepancy=46.51-2.51ANB-0.58AFH+0.65RH

콘크리트의 應力-變形關係에 關한 實驗的 硏究 : 鋼纖維 콘크리트에 關하여 About steel fiber concrete

金和中,權寧煥,李尙宰,全明熏 경북대학교 산업기술연구소 1990 産業技術硏究誌 Vol.18 No.-

The factors adopted in this experiment are volume fraction and the diameter and aspect ratio of fiber. The levels of factors are determined by existing studies. The effects of the diameter and mixing volume fraction and the aspect ratio for maximum strength, strain and tangent modulus are analyxed based on the linear multiple regression and we obtained regression equations as follows ; Maximum stress : σ_max=298.59820+95.78361φ-6.36043V_f+0.62067Ar Maximum strain at maximum stress : ε_max=0.021336+0.15746φ+0.05519V_f+0.00181Ar Tangent modulus : E=208209.80+87132.84φ-17286.69V_f-464.9181Ar where φ : diameter V_f : volume fraction Ar : Aspect Ratio

하수처리를 위한 전기화학 반응의 적용 : 최적처리조건의 결정;Determination of Optimal Treatment Conditions

김성호,김승도,신화섭,김영관,전양근 江原大學校 附設 環境硏究所 1999 環境硏究 Vol.16 No.-

본 연구의 목적은 전기화학방식의하수처리에 대한 적용 가능성을 검토하고, 주요 운전인자인전압, COD 부하량, pH, 전기전도도, 반응시간 등의 최적 처리조건을 결정하는데 있다. 각 웅전인자의 변화에 따른 COD의 처리효율을 비교 · 검토하여 최적조건을 도출하였으며, 대부분의 실험은 시간에 따른 COD의 변화를 측정할 수 있는 회분식 반응기를 사용하였고, COD 부하량의 영향을 평가하기 위해서는 체류시간을 변하시키면서 실험이 가능한 연속식 반응기를 사용하였다. 최적 조건은 전압 25V, COD 부하량은 약 160g COD/㎡ · day, pH는 중성범위, 전기전도도는 1000~1300㎲/㎝, 반응시간은 10분으로 나타났다. 전기응집반응이 탁월하여 불용성 COD 는 10분만에 90%이상이 제거되었으며, 대표적 응집제인 alum 100ppm을 투여한 경우보다 처리효율이 약 20 % 정도 향상되었다. 전기화학반응은 불용성 COD의 농도가 높은 하수처리 등에 적적한 것으로 판단되며 처리 시간이 빠르고, 경제성도 뛰어나 향후 하수처리기술로서 주목을 받게 될 것으로 판단된다. This research was carried out to test the feasibility of electrochemical method in sewage treatment and to determine the optimal conditions of operating parameters including applied voltage. COD loading. pH. Conductivity. And reaction time. They were determined based on the COD removal efficiency either by batch reactor operation or by continuous flow reactor operation. For monitoring the variation of COD with time a batch reactor was used, and continous flow reactor was operated at various detention times to estimate the effect of COD loading. The optimal applied voltage, COD loading, and conductivity was found 25V, approximately 160g COD/m² · day, and 1,000~1,300㎲/㎝, respectively. The appropriate pH value for proper operation was in neutral range. This process achieved over 90% of insoluble COD within 10min of reaction, and this efficiency was about 20% greater than when 100ppm alum solution was used for coagulation. It is expected that this electrocoagulation process may be best suited for treating gewage with high insoluble COD content. Good treatment efficiency and low operating cost could make this process gain an increased attention in the field of sewage treatment in not too distant future.

미립분 함유량에 따른 부순모래 콘크리트의 물성에 관한 실험적 연구

金鍾寅,崔榮和,全成泰 대구대학교 과학기술연구소 1999 科學技術硏究 Vol.5 No.5

This study, in order to comparing characters of fresh concrete and hardened concrete, was conducted on the crushed sand concrete with a water-cement ratios of 40, 50 and 60%, which was incorporated with 3, 5, 7, 10% of powder content. According to above tests, the major results of this study can be summarized as follows ; 1. As the powder content increased and the water-cement ratio became high , the slump loss and the slump flow loss appeared slightly small, the air content change and the unit weight change appeared small. 2. As the powder content increased, the compressive strength and the modulus of elasticity increased slightly due to the effect of void filling. And, the compressive strength is respectively increased about 43∼69, 8∼18, 20∼47kg/㎠ at the water- cement ratio of 40, 50, 60%. 3. As the powder content increased,. the length change by drying shrinkage of crushed sand concrete is increased.

늑막삼출액을 동반한 다발성골수종 1예

김용화,주기산,서영환,조경상,김상도,정춘해 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

Pleural effusion in a patient with multiple myeloma due to plasma cell involvement of the pleura has been reported infrequently. We report a case of multiple myeloma with pleural involvement presenting bone pain and dyspnea in a 69-year-o1d woman. On the physical examination, the patient looked chronically ill with bedridden state. There was a palpable mass on the left posterior lower chest, firm but not tender and decreased breathing sound in the left lower lung. Laboratory finding revealed amemia, elevation of ESR, globulin, and calcium. X-ray of the chest showed pleural effusion in the left lower lung, and multiple pathologic fractures in the both ribs. X-ray of the skull, and pelvis revealed multiple punched-out osteolytic fractures in the both ribs. X-ray of the skull, and pelvis revealed multiple punched-out osteolytic lesion, and compression fracture in the lumber spine. Serum and urine electrophoresis revealed a M-spike. Serum and urine immunoelectrophoresis demonstrated an IgA-kappa monoclonal protein. Thoracentesis revealed serosanguinous fluid but not containing atypical plasma cells. Bone marrow examination showed 16.7% of immature plasma cells. With first cycle of melphalan, prednisone chemotherapy, improvement of pain and disappearance of pleural effusion was observed.