Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

Rat의 복강에 삽입한 Filorouracil-Polyglycolic acid제형의 Fluorouracil용출에 관한 연구

노승무,정경수,오정연,김진향,양준묵,강대영,송규상,최정목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A common form of relapse in adenocarcinoma of the stomach is intraperitioneal dissemination, in fact, among gastric adenocarcinoma patients who have undergone surgery intended to cure, approximately 50% of the patients develope initial recurrence in the peritoneal cavity regardless of the anatomic site of the primary tumor within the stomach. The efficacy of systemic postoperative chemotherapy to prevent peritoneal recurrence of gastrric adenocarcinoma is not satisfactory. There is still a great need for improved therapeutic strategies on the disseminated microscopic disease and small miliary nodules remaining on the peritoneal surface or lymphatics after operation. The authers have made fluorouracil-polyglycolic acid composite disks(Fu-PGA disks) with fluorouracil and biodegradable polymer: polyglycolic acid for more effective intraperitoneal chemotherapy. We inserted the Fu-PGA disk(s) in the peritoneal cavity of rat and pharmacokinetic study was performed to measure fluorouracil concentration in the peritoneal fluid, plasma, liver, kidney and heart tissue at 24 hour, 72 hour and 168 hour after insertion of Fu-PGA disk(s). Myelosuppressive action of this composite also was determined following its administration. The data of this study suggested that Fu-PGA composite will be a new device releasing drugs in a controlled manner and having targetability to peritoneum, and this device will be improving the efficacy of intraperitoneal chemotherapy for gastric adenocarcinoma.

Rat의 복강에 삽입한 Fluorouracil-Polyglycolic acid 제형의 Fluorouracil 용출에 관한 연구

노승무,정경수,오정연,김진향,양준묵,강대영,송규상,최정목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

A common form of relapse in adenocarcinoma of the stomach is intraperitoneal dissemination, in fact, among gastric adenocarcinoma patients who have undergone surgery intended to cure, approximately 50% of the patients develope initial recurrence in the peritoneal cavity regardless of the anatomic site of the primary tumor within the stomach. The efficacy of systemic postoperative chemotherapy to prevent peritoneal recurrence of gastrric adcnocarcinoma is not satisfactory. There is still a great need for improved therapeutic strategies on the disseminated microscopic disease and small miliary nodules remaining on the peritoneal surface or lymphatics after operation. The authers have made fluorouracil-polyglycolic acid composite disks(Fu-PGA disks) with fluorouracil and biodegradable polymer: polyglycolic acid for more effective intraperitoneal chemotherapy. We inserted the Fu-PGA disk(s) in the peritoneal cavity of rat and pharmacokinetic study was performed to measure fluorouracil concentration in the peritoneal fluid, plasma, liver, kidney and heart tissue at 24 hour, 72 hour and 168 hour after insertion of Fu-PGA disk(s). Myelosuppressive action of this composite also was determined following its administration. The data of this study suggested that Fu-PGA composite will be a new device releasing drugs in a controlled manner and having targetability to peritoneum, and this device will be improving the efficacy of intraperitoneal chemotherapy for gastric adenocarcinoma.

金利現實化와 保險料率 : 生命保險을 中心으로

金振穆 청주대학교 1966 論文集 Vol.5 No.1

두부외상후 생존기간에 영향을 주는 요인분석

목직호,김한식,이규춘,이영배 동국대학교 경주대학 1997 東國論集 Vol.16 No.2

1995년 7월부터 1997년 7월까지 최근 2년간 본원 신경외과에 입원한 343례의 두부손상 환자중 사망한 25례를 조기사망군(7일이내)과 후기사망군(7일후)으로 분류하여 분석해본 결과 7.3%의 사망률을 보였으며 남자 사망자는 여자에 비해 3.2배였으며, 연령 별로는 조기사망군은 40대이상에서 많았고 후기 사망군은 60대 이상의 고령에서 다소 많았다. 조기사망군에서는 낮은 GCS, 비정상적인 동공반사 반응, 타장기 손상, 저혈압, 저산소증, 고혈당, 심한 정중전이, 뇌실압박, 두개골 골절, 개두술의 빈도가 높게 조사되었다. 이송시간은 조기사망군에서 1시간 이내에 도착한 경우가 많아 신속한 후송이 이루어졌다. 한편 후기사망군에서는 저이산화탄소혈증, 빈혈, 고나트륨혈증등이 높게 조사되었다. 사인은 조기사망군에서 1차성두개강내 병변 및 저혈량 등이 많았고 후기사망례는 폐렴, 성인성 호흡곤란 증후군, 외상후성 뇌경색증 등이 주요 사망 원인이었다. 따라서 생존기간별로 위험도가 높은 요소를 찾아 그에 대한 적극적인 치료를 하는 것이 두부 손상으로 인한 사망률을 개선하는데 바람직한 것으로 생각된다. We present a study the variable factor in the early(within one week) and late death(after one week) after head injury. The multiple factors were analysed on the clinical, imaging, and laboratory characteristics of 25 patients who were dead among 343 head injured persons during recent 2 years form July, 1995 to July, 1997. The mortality rate was 7.3% and the ratio of male to female was 3.2 to 1. The early death were younger than late death(5th decade vs 7th decade). Mechanism of injury were motor vehicle accident(64%), followed by work-related, slip and fall, and unknown. The early death had a higher percentage of low GCS score, abnormal pupil reflex, other associated injury, hypotension, hypoxemia, hyperglycemia, midline shift, ventricle compression, skull fracture, and craniotomy compared with the late death. On the other hand, the late death had a higher incidence of hypocarbia, anemia, and hypernatremia than the early death. The main causes of death were primary intracranial lesions and hypovolemia in the early death and pneumonia, ARDS, and posttraumatic cerebral infarction in the late death.

대두단백질 가수분해물의 특성

김동수,양승택,문윤희,류병호,김희숙,염동민,김진목 慶星大學校 1998 論文集 Vol.19 No.1

The present study was conducted to elucidate some characteristics of soy protein hydrolysates. The soy protein was obtained from defatted soy power by the treatment of n-hexan. The hydrolysates of soy protein were prepared from hydrolysis by bromelian and pepsin with the two kinds of samples of precooking for 30 min in steam oven and noncooking, and fractionated through Bio-gel P-4 column chromatography. Fractions of hydrolysates separated on the Bio-gel column chromatography were carried out to SDS-PAG electrophoresis, resulting to show some different bends from 66kD to 14.2kD of molecular weight. In addition, the fractions showed different activities of superoxide dismutase. From these results, it was found that the hydrolysates could be available to use in a lot of food industrial fields as new functional food materials because the hydrolysates had different characteristics in electrophoresis and could increase the activity of superoxide dismutase.

두개저 골절 환자의 임상적 고찰

김한식,목진호,이규춘,이영배 동국대학교 경주대학 1997 東國論集 Vol.16 No.2

저자들은 최근 2년간 본원 신경외과에 입원 치료 받았던 두부외상 환자 중 자료 분석이 가능했던 119례의 두 개저부 골절 환자를 대상으로 임상 분석하여 다음과 같은 결론을 얻었다. 두부외상후 두개저 골절의 발생 빈도는 34%로 남녀 발생비는 5 : 1이었고, 활동기 연령인 20∼59세가 70.6%를 차지 하였다. 원인으로는 교통사고가 56.3%로 가장 많고 추락, 산재, 낙상, 구타 순이었으며, 내원당시 환자상태는 경증뇌손상(GCS 13-15)이 64.7%, 중증 뇌손상(3∼8) 21.8%, 중등도 뇌손상(9∼12) 13.5% 순이었고 예후도 GCS가 높을수록 양호하였다. 임상소견은 뇌척수액 비루(43.7%)가 가장 흔하고 비루(31.9%), 안와부 반상출혈(31.1%), 기뇌증(26.1%), 청각소실(18.5%)등이었다. 뇌척수액루의 지속 기간은 87.7%가 7일 이내였고 보존적 치료로 93.9%가 해결되었다. 2∼4주 지속된 경우는 요추천자 배액술, 4주 이상된 경우는 수술을 시행하였다. 뇌신경 손상은 안면신경마비가 31.7%로 가장 높았고 발현 시기는 지연성 마비가 69.2%, 조기마비가 30.8%였다. 뇌막염의 발생 빈도는 5.9%로 모두 뇌척수액루가 1주 이상 지속된 경우였고, 항생제 투여군과 비투여군의 뇌막염 발생율은 각각 6.1%와 4.8%로 항생제가 감염율을 낮추지는 못했다. 119 consecutive cases with basal skull fractures out of 343 head injuried patients who were treated in Pohang Hospital from July 1995 to July 1997 were analysed. The authors reviewed the clinical features, radiologic findings, complications and outcomes. Most of the patients(70.6%) were between 20 and 59 years old. The basal skull fractures were more common in men than women-the ratio being 5 : 1. The cause of BSF were traffic accident(56.3%), fall down, work related, silp down, and assults in decreasing order of frequency. The outcome of treatment wee related to initial GCS(Glasgow coma scale) of patients. The severity of head injury were mild(GCS13-15)-64.7%, Moderate(GCS9-12)-13.5%, and severe(GCS3-8)-21.8%, The most common feature of BSF were rhinorrhea(43.7%), otorrhea(31.3%), raccoon's eye(31.1%), pneumocephalus(26.1%), hearing disturbance(18.5%). Most of CSF leakage(86.7%) were noted within 24hours after injury and most of leakage(93.9%) were ceased by conservative management within 2 weeks, but one case that did not respond to conservative and lumbar drainage required operative repair. The facial nerve(31.7%) was the most frequently involved cranial nerve followed by vestibulocochlear and optic nerve. The onset of facial palsies were immediate in 30.8% and delayed 69.2%. The incidence of meningitis was 5.9% and the prophylactic antibiotics had no beneficial effect in reducing the infection rate.

Self-Leveling재의 최적 결합재비 산정을 위한 기초적 연구

김진만,조성현,조헌영,한기석,서정목 공주대학교 방재연구센터 1998 공주대학교 방재연구센터 논문집 Vol.2 No.-

Self-leveling material is the floor finishing material that is able to make surface level by itself, it can go on a next construction in a short time. Self-leveling materials are composed of various binders and chemical additions, and it is difficult to catch suitable mixing proportion of composion materials. This study is to investigate and analyze consistency and strength properties of according to component ratio of main materials and we endeavor to get suitable mixing propertion of binders by experimental design and statistical analysis. The results of this study are showed that suitable mixing proportions of binders are OPC(Ordinary Portland Cement) 70~80%, AC(Alumina Cement) 5~10%, AG)Anhydrous Gypsum) 5~20%.

후두와 병변 수술의 발생한 경막하 긴장성 기두증

목진호,김한식,이규춘,박용석,이영배 東國大學校醫學硏究所 1999 東國醫學 Vol.6 No.-

긴장성 기두증은 신경외과영역에서 개두수술 후에 발생할 수 있는 드문 합병증으로 특히 만성 뇌경막하 혈종 제거수술이나 좌위에서 후두와 수술을 시행한 경우에 발생할 수 있다. 따라서 개두수술 후에 신경학적 증상의 악화가 있거나 수술 후 예상경과를 취하지 않는 경우에는 긴장성 기두증의 발생 가증성을 항상 고려하여야 한다. 긴장성 기두증의 발생기전은 물병을 거꾸로 세운 것과 같은 기전(Inverted pop-bottle mechanism)으로 뇌척수액이 누수된 만큼 공기방울이 경막하강으로 올라가 빈 공간을 메우는 것이다. 이와 같은 기전으로 두개강내로 들어간 공기가 일정한 압력을 가지게 되어 주변 뇌조직을 압박하여 신경학적 장애를 초래하게 된다. 뇌전산화 단층촬영을 이용하여 쉽게 진단이 가능하며 치료는 두개골 천공을 시행하여 두개강내 공기를 배기시켜 줌으로서 가능하다. 저자들은 복와위에서 후두와 병변 수술 후에 발생한 긴장성 기두증을 뇌전산화 단층촬영을 이용하여 진단하고 전두골을 천공하여 두개강내 공기를 배기시켜 치험하였기에 문헌고찰과 함께 증례를 보고하는 바이다. 중심단어:긴장성 기두증, 후두와 수술, 경막하강, 복와위 Tension pneumocephalus is a rare complication in neurosurgical operation and should be considered whenever a patient fails to recover as expected following surgery. The possible mechanism for the entry of air into the subdural space is analogous to the entry of air into an inverted soda-pop bottle. As the fluid pours out. air bubbles reach the top of the container, Computed tomography permitted rapid diagnosis including localization of the air, thus facilitating prompt treatment. Peaking of frontal lobe. mountain appearance of frontal lobe. and air densities at the cisterns are characteristic findings of' tension pneumocephalus on brain CT scan. This condition is easily diagnosed and treated using twist-drill aspiration. The authors describe a case of subdural tension pneumocephalus complicating the early postoperative course after posterior fossa surgery in the prone position.