Effect of Growth Rate on the Production of L-Proline in the Fed-batch Culture of Corynebacterium acetoacidophilum

안정오,Jun-ki Jung,Jimyung Ryu,Hyungwook Jang 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.4

Corynebacterium acetoacidophilum RYU3161 was cultivated in a L-histidine-limited fed-batch culture. To investigate the effect of cell growth on the L-proline production, 5 L fed-batch culture was performed using an exponential feeding rate to obtain the specific growth rates () of 0.04, 0.06, 0.08, and 0.1 h-1. The results show that the highest production of L-proline was obtained at = 0.04 h-1. The specific L-proline production rate (QP) increased proportionally as a function of the specific growth rate, but decreased after it revealed the maximum value at = 0.08 h-1. Thus, the highest productivity of L-proline was 1.66 g L-1 h-1 at = 0.08 h-1. The results show that the production of L-proline in C. acetoacidophilum RYU3161 has mixed growth-associated characteristics.

Effect of Growth Rate on the Production of L-Proline in the Fed-batch Culture of Corynebacterium acetoacidophilum

Ahn, Jung-Oh,Ryu, Jim-Yung,Jang, Hyung-Wook,Jung, Jun-Ki The Korean Society for Biotechnology and Bioengine 2004 Biotechnology and Bioprocess Engineering Vol.9 No.4

Corynebacterium acetoacidophilum RYU3161 was cultivated in a L-histidine-limited fed-batch culture. To investigate the effect of cell growth on the L-proline production, 5 L fed-batch culture was performed using an exponential feeding rate to obtain the specific growth rates $(\mu)$ of 0.04, 0.06, 0.08, and 0.1 $h_1$. The results show that the highest production of L-proline was obtained at $\mu$ = 0.04 $h_1$. The specific L-proline production rate $(Q_p)$ increased pro-portionally as a function of the specific growth rate, but decreased after it revealed the maxi-mum value at $\mu$ = 0.08 $h_1$. Thus, the highest productivity of L-proline was 1.66 g $L^-^1 h^-^1$ at $\mu$ = 0.08 $h_1$. The results show that the production of L-proline in C. acetoacidophilum RYU3161 has mixed growth-associated characteristics.

기저질환이 없는 사람에서 발생한 Campylobacter fetus에 의한 재발성 심내막염 1예

전재범,정진원,김성혜,송문희,오형철,안성기,최상호,김남중,김양수,우준희,류지소 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

C. fetus 심내막염은 드물게 발생하는 질환으로 주로 면역능이 저하된 환자에서 발병하고 대동맥판을 침범하는 경우가 많으며 적절한 치료가 되지 않을 경우 치사율이 높다. 저자들은 기저질환이 없던 69세 남자에서 C. fetus 심내막염을 경험하였기에 문헌고찰과 함께 보고하는 바이다. 환자는 발열로 내원하여 시행한 혈액배양 검사상 C. fetus가 동정되었고 경식도 초음파 검사상 삼첨판에 증식증이 발견되어 심내막염 진단하에 ceftriaxone에 이어 meropenem으로 총 4주간 항생제를 투여받고 호전되었다가 한달 후 다시 재발하였고 판막부전 소견 진행하여 삼첨판 치환술을 시행받고 전신상태 호전되어 퇴원하였다. Campylobacter fetus is a rare cause of endocarditis. To date, only about 30 cases of C. fetus endocarditis have been reported mainly in the elderly with chronic underlying illnesses. Most cases showed tendency to involve aortic valve. This report describes a case of native tricuspid valve infective endocarditis in a 69-year-old man without any underlying disease. Despite adequate antibiotic therapy for 4-weeks, endocarditis recurred and leading to surgical valve replacement.

두경부 편평세포암에서 미세혈관밀도와 경부림프절전이에 대한 연구

류준선 동국대학교 경주대학 2000 東國論集 Vol.19 No.-

Lymph node metastasis is believed to be the single most important prognostic factor in the head and neck squamous cell cancer. To identify potential biological parameters for predicting cervical lymph node metastasis, we evaluated the relationship between cervical nodal status and several parameters, such as microvessel density, p53, Ki67, and DNA ploidy, and compared with conventional clinical parameters including histologic grade and TNM staging of the tumors. This study group included 26 specimens of the primary site in patients who were diagnosed as squamous cell cancers of the head and neck. Recurred cases after chemotherapy or radiotherapy were excluded from this investigation. Analysis was performed by immunohistochemstry and DNA flowcytometry at the almost same section of the primary sites. To quantify the angiogenesis of the primary site, the immunohistochemical staining with CD-31 monoclonal antibody was performed. Microvessel density was determined by counting of CD-31 positively stained vascular endothelial cells under the magnification of x 200 field by two investigators, at an average of the largest numbers after 3 times of digitizing. Immunohistochemical staining with Ki67 and p53 were also done to evaluate the cellular proriferation of tumors and the overexpression of mutated tumor suppresor gene. DNA flowcytometry was performed to evaluate the ploidy and proliferation index. These results were compared and analyzed with clinical parameters. Any parameters failed to show significant relation to nodal status in the head and neck cancers. However, the microvessel density of the laryngeal cancers showed statistical significancy with cervical nodal metastasis. (p=0.045) Therefore, the microvessel density may have a relationship to lymph nodal metastasis in head and neck squamous cell cancer and might be used as an additional prognostic factor for planning treatment.

코치의 직무 스트레스에 관한 비교연구

류경희,함경수,황준섭 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The main objective of this study is to reveal the relationship between the cause of a coach's job stress. In terms of the subject of this study, coaches, who are now at work in Kyunggi Province 2003, were selected as a population, especially 177 persons of coaches were adopted by means of nonproportional stratified sampling. In this survey, demographical variables are sex, age, education, job character, training item and coaching experience, in addition to them, with regard to dependent variables there are precisely not only role ambiguity, role conflict, excessive work, personal relations, expenses problem and work conditions as the cause of job stress, but also job satisfaction and tendency for turnover as the system efficiency. According to those kinds of method and procedure, the results of this study are as follows. 1) The difference of cause of job stress in sex is showed that male group is statistically more significant than female group. 2) The difference of cause of job stress in ages is present that group in forties is more significant than group of in twenties or thirties. 3) The difference of cause of education is significant only between group of the high school-educated and group of the graduate school-educated in terms of work conditions(p=0.0001). 4) The difference of cause of coaching experience is displayed that group of the 6-year-experienced over is much more significant than group of group of the 5-year-experienced below.

자동차 실내 환경 감성측정기 개발 및 공조제어 모델링

류정호,주병수,김봉균,임준우,이덕동 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

A sensibility measurement system that can measure human sensibility at the automobile cabin environment is developed in this paper. Five kinds of sensors that can measure temperature, humidity, oxygen, CO_(2), and HC gas concentrations are used in this system. Sensibility indexes are defined for each sensor datum, and then those are linearly combined to make a total human sensibility index. A stand-alone system is manufactured using a one-chip microprocessor, and simple controls are showed with LEDs. This system was tested for performance in automobile cabin environment and showed good reliability.

레진 세멘트와 포스트의 길이가 근관 치료된 치아의 포스트 유지력에 미치는 영향

류준욱,권정애 慶北大學校 齒科大學 1993 慶北齒大論文集 Vol.10 No.2

근관치료를 한 치아에 포스트를 해야할 경우 세멘트의 종류나 세멘트되는 포스트 길이가 포스트의 유지에 어떠한 영향을 주는지 알아보기 위하여, 하악 절치 30개를 이용하여 법랑-백악질 경계 선상에서 치아장축에 수직되게 절단한 후 근관치료 및 근관충전을 하였다. 기성품의 parallel Para-post를 샌드브라스팅하여 Ⅰ군에서는 깊이 4mm에 Z.P.C., Ⅱ군에서는 깊이 8mm에 Z.P.C., Ⅲ군에서는 깊이 4mm에 All-Bond, Ⅳ군에서는 깊이 8mm에 All-Bond로 세멘트 하였다. 3일이 경과후 인스트론 만능시험기에 retention jig를 사용하여 포스트가 세멘트된 시편을 거상하고 cross head speed 1mm/min으로 인장력을 가하여 유지력을 측정하고 포스트 공간 형성 후, All-Echant처리 후 파절된 Ⅲ군과 Ⅳ군의 치근내면 및 포스트와 레진의 접합면을 주사전자현미경을 사용해서 관찰하여 다음과 같은 결과를 얻었다. 1. 각 군의 평균 파단 하중은 Ⅳ군에서 23.80±4.17kg, Ⅱ군에서 17.99±5.5kg, Ⅲ군에서 16.68±5.13kg, 그리고 Ⅰ군에서 10.20±4.08kg의 순으로 나타났다. 2. Z.P.C.를 사용한 Ⅰ군과 Ⅱ군 사이에는 Ⅱ군이 높게 나타났으며(P<0.05), All-Bond를 사용한 Ⅲ군과 Ⅳ군 사이에도 Ⅳ군이 높게 나타났다(P<0.05) 3. 포스트의 길이에서 4mm의 Ⅰ군과 Ⅲ군 사이에는 Ⅲ군이 높게 나타났고(P<0.05), 8mm의 Ⅱ군과 Ⅳ군 사이에는 Ⅳ군이 높게 나타났다(P<0.05) 4. 주사전자현미경 관찰에서 All-Bond를 사용한 군에서 치경부 부위에 많은 레진 tag를 관찰할 수 있었으나 치근단 부위로 갈수록 레진 tag를 관찰할 수 없었다. The purpose of this study was to evaluate the effect of various cements & the length of post to retention of post in the endodontically treated teeth. Thirty three mandibular incisors were used. The coronal portion of the teeth were removed at the cemento-enamel junction. Every tooth was endodontically treated. After 2 days, post space preparation was made with Para-post drill(P-42-1) to depth of 4mm & 8mm. The teeth were divided into four groups. Group Ⅰ was composed of 8 mandibular incisors and posts were cemented with zinc phosphate cements to depth of 4mm. Group Ⅱ was composed of 8 mandibular incisors and posts were cemented with zinc phosphate cements to depth of 8mm. Group Ⅲ was composed of 8 mandibular incisors and posts were cemented with All-Bond to depth of 4mm. Group Ⅳ was composed of 8 mandibular incisors and posts were cemented with All-Bond to depth of 8mm. Special retention jig was made. After 3 days,teeth with cemented post were mounted on retention jig and failure load of the specimens were measured by an Instron Universal Testing Machine. And,scanning electron microscopy was examined on the internal surface of root after post space preparation, treatment of All-echant, internal surface of fractured group Ⅲ & group Ⅳ, and interfacial surface of post & resin. The results were as follows. 1. Mean failure loads of each group was 23.80±4.17kg in group Ⅳ,17.99±5.5kg in group Ⅱ,16.68±5.13kg in group Ⅲ,and followed by 10.20±4.08kg in group Ⅰ. 2. There was a statistically significant difference between group Ⅰ & group Ⅱ and between group Ⅲ & group Ⅳ(P<0.05). The score of group Ⅰ was higher than group Ⅱ(P<0.05). And,that of group Ⅳ was higher than group Ⅲ(P<0.05). 3. In post length, group Ⅲ was statistically higher than group Ⅰ and group Ⅳ was statistically higher than group Ⅱ(P<0.05). 4. In examination of scanning electron microscopy, groups Ⅳ used All-Bond was examed many resin tags in cervical portion, but there was no resin tag in apical portion and group Ⅲ was examed many rsin tags. J. Kyungpook Nat. Univ. Sch Dent Vol10, NO.2,

신증후군, 단클론성 감마병증 및 다발성 골용해 병변으로 발현한 소형 림프구성 림프종 1예

유준호,안명주,김호중,이웅수,박용욱,박문향 한양대학교 의과대학 1999 한양의대 학술지 Vol.19 No.2

We reported here a 70-year-old woman with small lymphocytic lymphoma who was initially presented with recurrent nephrotic syndrome and later developed multiple bone lytic lesions and monoclonal gammopathy, mimicking plasma cell dyscrasia and only involving bone marrow without lymphadenopathy or hepatosplenomegaly. She responded to combination chemotherapy, including cyclophosphamide and prednisone with proteinuria disappearing. The unusual features of this case includes : (1) nephrotic syndrome of minimal change lesion as the initial manifestion in lymphocytic lymphoma, (2) only bone marrow involvement of lymphoma without organomegaly or lymphadenopathy, (3) the presence of multiple bone lytic lesions in conjunction with monoclonal gammopathy in a patient with lymphoma rather than multiple myeloma.

Electrosorption and Separation of Co²and Sr²Ions from Decontaminated Liquid Wasres

Jun0Soo Kim,Chong-Hun Jung,Won-Zin Oh,Seung-Kon Ryu 한국탄소학회 2002 Carbon Letters Vol.3 No.1

성인원외폐렴의 원인미생물에대한 전향적 다기관 연구 : 성인원외폐렴의 원인으로 세균의 역할을 중심으로

우준희,강재명,김양수,신완식,류진홍,최정현,김양리,정희진,어수택,박춘식,정문현,정기석,이찬주,류지소 대한감염학회 2001 감염 Vol.33 No.1

Background : Communite-acquired peumonia (CAP) is one of the leading causes of mortality and morbidity. Despite progress in diagnostic techniques and treatments, management of pneumonia remains challenging, because the precise etiology remains uncertain in as many as 49% of cases. The limitaions of identifying etiologic agents make it necessary to use empiric antibiotics in almost all patients, and furthermore emergence of antibiotic-resistant organisms pose difficulties to the selection of an empiric antibiotic regimen. For the optimal choice of empirical antibiotics, we should know the frequency of etiologic agents and antimicrobial resistance rates in the community. Methods : A prospective multicenter study of community-acquired pneumonia in Korea was carried out between May 1997 and April 2000. The microbiologic diagnosis was based on the results of sputum culture, blood culture and pleural culture. Results : Five hundred eighty eight cases of community-acquired peumonia in 562 patients admitted to the hospitals. The mean age was 59.9 with male predominance (58.3%), and 370 (63%) had underlyin gillness. The etiologic agents were identified in 38.3%, and the list of individual agents, in decreasing order, was Streprococcus pneumoniae (21.7%), Klebsiella pneumoniae (14.8%) Pseudomonas aeruginosa (9.8%), Staphylococcus aureus (9.5%), viridans group streptococci (5.7%), Enterobacter cloacae (4.2%), Hemophillus Influenza (3.8%), The rates of admission to the intensive care unit was 10.4%. The motality was 7.1%. Susceptible rates of S. pneumoniae to penicillin was 36.6% and showed multidrug resistant. Forty percents of S. aureus were methicillin-resistant S. aureus. K. penumoniae were susceptible to cephalosporin and quinolone. Conclusion : In Korea, S.pneumoniae is the most important agent causing community-acquired pneumonia. Susceptible rates of S. pneumoniae to penicillin was 36.6% and showed multidrug resistant. Gram negative bacteria such as K. pneumoniae, P. aeruginosa showed high incidence when compared with that of other countries. Most of them had underlying diseases including bronchiectasis and chronic obstructive pulmonary diseases. (Korean J Infect Dis 33:1∼7, 2001)