Effect of Feed Selenium-lysine Supplementation on Milk Compositions and Serum Biochemical Indices in Saanen Dairy Goats

Tae-Il Kim,Dong-Hyun Lim,Tai-Young Hur,Seung-Min Ha,Hyun-Jong Kim,Seong-Min Park,Ji-Hoo Park,Sang-Bum Kim,Ji-Hwan Lee,Hyun-Joo Lim,Jeong-Sung Jung,Ha-Yeon Jeong,Jay Lee,Kwang-Seok Ki,Vijayakumar Mayak 경상대학교 농업생명과학연구원 2019 농업생명과학연구 Vol.53 No.4

An experiment was carried out to assess the effect of feed selenium-lysine (Se-Lys) supplementation on milk compositions and serum biochemical indices in Saanen dairy goats in Korea. A total of twelve 36 months old Saanen lactating dairy goats (47±6.21 kg) fed the similar dry matter intake twice a day at 2% of BW (DMI) (10.9% moisture of concentrate and 19% moisture of roughage), milk yield (2.5 kg/d) and parity (2) were randomly selected and subjected for the present study, divided into two groups with six goats in each group. The goats in the control group received rice hulls (10 g/ day) only, and did not receive Se-Lys; goats in the treatment group were fed 0.06 g of Se-Lys with 10 g of rice hulls every day before feeding roughage for six weeks. The milk sample was collected every week, and its compositions were analyzed. The results of the present study showed that there is no significantly increased milk production in Se-Lys treated group goats when compared with control group goats. But, Se-Lys treatment significantly increased the milk protein content (3.98±0.16%), fat (3.72±0.27%), lactose (4.07±0.14%), total solids (12.51±0.28%) and urea (14.42±1.45 mg/dl) content as compared to the control group goats (p<0.05). The somatic cell counts (207,740±28.81 cells/ml) were significantly lower in the Se-Lys treated group than in the control group (p<0.05). Also, the results of the current study showed that supplementation of Se-Lys were significantly decreased the blood biochemical indices of IL-6 (34.34±6.04 pg/ml), TNT-α (0.56±0.22 ng/ml), MDA (5.07±1.03 ng/ml), GPx-1 (9.07±5.17 ng/ml), sCD4 (2.64±1.02 ng/ml) and sCD8 (5.08±2.08 ng/ml) level when compared with without addition of Se-Lys group dairy goats (p<0.05). On the other hand, the selenoprotein P (1,580.18±127.62 ng/ml) level was significantly higher in Se-Lys supplemented group than in the control group (p<0.05). Based on the study results, it was concluded that feed Se-Lys supplementation may improve milk yield with positively improved protein, fat, lactose, total solids, urea content, and biochemical indices without negative effects on milk production traits.

초·중·고 학생들의 과학 탐구 능력 추이 분석을 위한 종단적 연구

우종옥,김범기,허명,김찬종,양일호,최관순,김태선 한국교원대학교 과학교육연구소 1999 청람과학교육연구논총 Vol.9 No.1

본 연구에서는 우리 나라 초·중·고등학교 학생들의 과학 탐구 능력에 대한 종단적 추이를 분석하였다. 1997년에는 우종옥 등(1998)이 개발한 검사지를 사용하였으며, 1999년에는 이를 일부만 수정하여 사용하였다. 조사 대상은 성별, 지역별 등을 고려하여 표집하였다. 조사된 자료는 각각 횡단적으로 분석하고, 이를 바탕으로 학생들의 과학 탐구능력이 어떻게 변해 가는지 종단적 추이를 분석하였다. 조사 결과 초등학생들의 과학 탐구 능력은 유의미한차이가 없었으나, 중·고등학생들의 과학 탐구 능력은 유의미하게 향상된 것으로 나타났다. 학교 과학 교육의 질을 과학적으로 관리하기 위해서는 교육과정이 추구하는 교육 목표의 달성이 성공적으로 이루어지고 있는지를 국가수준에서 주기적으로 측정하여 부진의 원인을 진단하고, 개선 방안을 계속 연구하여야 할 것이다. The longitudinal trend of elementary and secondary students' science process skills were investigated to monitor the progress of science learning. Science process skill test, developedin an earlier study on developing a national science process skills assessment system, was revised and administered for the study on March, 1999. Subjects were sampled nation wide according to sex and region. The results of this assessments were compared to those administered on February, 1997. The results showed little difference on science process skills among elementary school students. However, secondary students showed marked increase in science process skills. More systematic and long-term research for assessing science process skills should be planned and performed periodically to provide empirical and foundational data for educational policy making.

산삼 배양액 급여가 돈육의 이화학적 특성에 미치는 영향

진상근,김일석,송영민,허선진,박기훈,정기종,김동훈,노정만 진주산업대학교 산업과학기술연구소 2006 산업과학기술연구소보 Vol.- No.13

Berkshire를 이용하여 70일간 산삼 배양액 급여 시 육의 이화학적 특성에 미치는 영향은 다음과 같다. 산삼 배양액의 급여에 의해 육의 수분, 조단백, 조지방, 회분, pH, 가열감량, 콜레스테롤 함량, 육색, 조직감의 응집성과 검성, 포화지방산 및 불포화지방산의 비율은 차이를 보이지 않았다. 보수력, 지방색 L^(*)값, 지방산 중 linoleic acid(18:2), arachidonic acid(20:4), 필수지방산의 함량, 아미노산 중 threonine, glutamic acid, valine, isoleucine, leucine, histidine, lysine 및 총아미노산 함량은 산삼 배양액의 급여에 의해 감소하였다. 전단가, 조직감의 표면경도, 경도, 씹힘성, 지방산 중 stearic acid(18:0), oleic acid(18:1) 함량, 아미노산 중 serine, glycine, cystine, methionine, tyrosine 및 phenylalanine의 함량은 산삼 배양액의 급여에 의해 증가하였다. 본 연구결과를 종합하면 산삼 배양액의 급여는 돈육의 경도를 증가시키는 효과 외에 뚜렷한 이화학적인 차이를 나타내지 않았다. 이러한 이유는 급여된 산삼 배양액 내에 함유된 생리활성 물질의 함량이 낮고, 급여수준이 낮은 결과로 사료되어진다. A total of 60 pigs (Berkshire) were used to investigate the effect of dietary artificial culture medium of wild ginsengs (CMG) on physico-chemical characteristics of pork. About 60 kg pigs were randomly alloted into one of two experimental diet groups (C:commercial diet feed; T:commercial diet + 1 L CMG per day for 70days). Pigs were slaughtered at approximately 110 kg live weight, and chemical composition and physico-chemical characteristics were measured in pork loin. Moisture, crude protein, crude fat and ash were not significantly different by dietary artificial culture medium of wild ginsengs. Water-holding capacity was decreased by dietary artificial culture medium of wild ginsengs, while shear force was increased. However, pH, cooking loss and cholesterol contents were not significantly different between two different treatments. In color, L^(*) of fat was decreased by dietary artificial culture medium of wild ginsengs. Brittleness, hardness and chewiness were increased in dietary artificial culture medium of wild ginsengs group. In fatty acid, stearic acid (18:0) and oleic acid (18:1) were higher in dietary artificial culture medium of wild ginsengs group, whereas linoleic acid (18:2) and arachidonic acid (20:4) were higher in control group. In amino acid, serine, glycine, cystine, methionine, tyrosine and phenylalanine were higher in dietary artificial culture medium of wild ginsengs group, whereas threonine, glutamic acid, valine, isoleucine, leucine, histidine and lysine were higher in control group.

Staphylococcus aureus와 Coagulase-Negative Staphylococcus Species에 대한 Arbekacin의 시험관내 항균력

위성헌,강진한,허동호,이동건,김상일,김양리,최정현,김종현,유진흥,허재균,신완식,강문원 대한감염학회 2001 감염 Vol.33 No.4

Background : Most strains of methicillin-resistant Staphylococcus aureus (MRSA) now exhibit high-level resistance to various antibiotics, such as β -lactam antibiotics, aminoglycosides, macrolides, tetracyclines and quinolones. Recent reports describing the therapeutic failure of vancomycin for MRSA infections have arisen considerable concerns regarding the emergence of MRSA strains, which will require new therapeutic agents. Arbekacin, an aminoglycoside antibiotic, has antibacterial activity against both gram-positive and gram-negative bacteria and is stable in the presence of aminoglycoside inactivating enzymes produced by S. aureus. In this study, we compared the antibacterial activity of arbekacin with those of vancomycin, gentamicin, and amikacin against Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CNS). Methods : For a collection of 549 S. aureus and 251 CNS isolates from three Catholic University Hospitals in Korea, minimum inhibitory concentrations (MICs) of arbekacin, vancomycin, amikacin and gentamicin were determined by agar dilution method using Mueller-Hinton agar according to NCCLS (National Committee for Clinical Laboratory Standards, USA)criteria. Results : Among 549 S. aureus isolates, 278 isolates were MRSA and 271 isolates were methicil sensitive S. aureus (MSSA). MIC50 & MIC90 of arbekacin against 549 S. aureus were 0.5 & 1 ㎍/mL, and MIC50 & MIC90 of vancomycin were 1 & 1 ㎍/mL. MIC of arbekacin against 549 S. aureus isolates ranges from 0.03 to 4 ㎍/mL, and MIC of vancomycin against 549 S. aureus ranges from 0.25 to 2 ㎍/mL. MIC90 of amikacin against 549 S. aureus was 32㎍/mL, and that of gentamicin was 128 ㎍/mL. MICs of amikacin and gentamicin were variable, ranging from 0.125 to 256, and otherwise arbekacin and vancomycin revealed relatively narrow range of MICs. MIC90 of arbekacin against 278 MRSA isolates & 271 MSSA were 1 & 0.5 ㎍/mL, and those of vancomycin against MRSA & MSSA were 1 & 1 ㎍/mL. MIC90 of amikacin against 278 MRSA & 271 MSSA isolates were 32 & 4 ㎍/mL, and that of gentamicin against MRSA & MSSA isolates were 128 & 32 ㎍/mL respectively. Among 251 CNS isolates, 122 isolates were MRCNS and 129 were MSCNS. MICSO & MIC90 of arbekacin against 251 CNS isolates were 0.25 & 2 ㎍/mL, and those of vancomycin were 1 & 2 ㎍/mL. MIC of arbekacin against 251 CNS isolates ranges from 0.015 to 32 ㎍/mL, and that of vancomycin isolates ranges from 0.25 to 2 ㎍/mL, MIC90 of arbekacin against 122 MRCNS & 129 MSCNS isolates were 2&0.3 ㎍/ML, and those of vancomycin were 2&s ㎍/ML. MIC90 of amikacin against 251 CNS isolates was 32 ㎍/ML, and that of gentamicin was 128 ㎍/ML for CNS. MIC90 of amikacin against 122 MRCNS & 129 MSCNS isolates were 128 & 8㎍/mL, and those of gentamicin ere 256 & 32 ㎍/mL. Conclusion : Considering above results, arbekacin can be useful agent against most strains of MRSA and MRCMS, which exhibit high-level resistance to amikacin and gentamicin. (Korea J Infect Dis 33:254~260, 2001)

Multi-media and multi-pathway aggregate exposure assessment for polycyclic aromatic hydrocarbons to the inhabitant in Tae-an county after Hebei-spirit oil spill

Kim Chan-Kook(김찬국),Woo-Chul Jeong(정우철),Jong-il Hur(허종일),Pan-Soo Park(박판수),Tae-Woo Lee(이태우),Hwi-Soo Seol(설휘수),Jung-hyeon Park(박정현),Jong-Heop Lee(이종협),Jong-Ho Kim(김종호),Byeong-Kyu Kwak(곽병규),Jong-Hyeon Le 한국환경독성학회 2010 한국독성학회 심포지움 및 학술발표회 Vol.2010 No.11

전주근교 농장의 자돈에서 면역형광항체법을 이용한 돼지콜레라 혈청 중화항체가 조사

허창열,김영진,노수일,김진구,송희종,김종면 한국수의공중보건학회 1991 예방수의학회지 Vol.15 No.1

Nitrogen Use and Yield of Silage Corn as Affected by Hairy Vetch (Vicia villosa Roth) Soil-incorporated at Different Time in Spring

Jong-Ho Seo,Ho-Jin Lee,Il-Bong Hur,Si-Ju Kim,Chung-Guk Kim,Hyeon-Suk Jo,Jung-Sam Lee 韓國作物學會 2000 Korean journal of crop science Vol.45 No.4

Winter green manure crops including legume increase grain yield of subsequent crop and substitute N fertilizer requirement with organic-No Hairy vetch grows vigorously and can provide N-rich green manure for corn with its soil incorporation after wintering. But, grain yield of corn as succeeding crop would be reduced if its planting time is delayed until late spring. This experiment was carried out to find the proper incorporation time of hairy vetch green manure and planting time of subsequent corn in cropping system with winter hairy vetch(green manure)-summer corn. Hairy vetch was incorporated into soil at a ten-day interval between April 10 and May 10 and corn was planted at 5 days after each hairy vetch incorporation. Soil nitrate concentration on April 10 and 20 in hairy vetch plot was slightly lower than that at winter fallow. Above-ground dry matter and organic-N of hairy vetch increased linearly with delayed hairy vetch incorporation time from April 10 to May 10. Average dry matter and organic-N produced by hairy vetch were 5.7 ton/ha and 248 kgN/ha, respectively. Corn growth and yield decreased as delayed corn planting time after May in spite of increasing dry matter and N-yield of hairy vetch. Nitrogen concentration of corn grain, stalk and whole plant at harvest were the highest in May 5 planting, but total N-uptake of May 5 planting were not different from that of April 25 planting because of lower grain yield. It was concluded that the proper incorporation time of hairy vetch and corn planting time were April 20 and April 25, respectively, because grain yield was the highest and corn could use hairy vetch-N effectively to produce dry matter.

Use of Hairy Vetch Green Manure as Nitrogen Fertilizer for Corn Production

Jong-ho Seo,Ho-jin Lee,Il-bong Hur,Si-ju Kim,Chung-kuk Kim,Hyeon-suk Jo 韓國作物學會 2000 Korean journal of crop science Vol.45 No.5

Hairy vetch (Vicia villosa Roth) winter annual is very effective on reducing chemical nitrogen fertilizer for subsequent com by fixed organic green manure nitrogen fixed during hairy vetch growth. In this experiment, hairy vetch produced above-ground dry matter of 5 ton/ha, nitrogen yield 200 kgN/ha, at com planting on the average during 1997 and 1998. Changes in com yield and nitrogen uptake for two years were investigated after application of nitrogen fertilizer 0, 60, 120, 180, 240 kgN/ha on plot of winter fallow and hairy vetch green manure, respectively. Nitrogen status such as ear-leaf N%, SPAD value at silk and dough stage, and com yield decreased in proportion to reduction of nitrogen fertilizer at winter fallow, but nitrogen status and yield of com were not different among nitrogen fertilizer rate at hairy vetch green manure. Com yield (total dry matter) at 0 kgN/ha plot of hairy vetch was 22, 20 ton/ha in 1997, 1998, respectively and com could produce more dry matter 9, 13 ton/ha by hairy vetch green manure compared with winter fallow under the condition of no nitrogen fertilizer in 1997, 1998, respectively. Com yield (total dry matter) at 60kgN/ha of hairy vetch green manure was higher than that of high N fertilizer rate such as 180, 240 kgN/ha of winter fallow. Nitrogen uptake of com at plot of hairy vetch-no nitrogen fertilizer slightly decreased than at plot of hairy vetch - nitrogen fertilizer, but com absorbed more nitrogen of 141, 159 kgN/ha by hairy vetch green manure compared with winter fallow under no nitrogen fertilizer condition in 1997, 1998, respectively. Nitrogen fertilizer reduction for com by hairy vetch green manure was 149, 161kgN/ha in 1997, 1998, respectively. Still more, com could absorb more soil nitrogen by nitrogen fertilizer 60kgN/ha of hairy vetch green manure than by high nitrogen fertilizer such as 180, 240 kgN/ha at winter fallow. It is concluded that nitrogen fertilizer for corn could be reduced by winter cultivation and soil incorporation of hairy vetch at com planting.

기관지 결핵과 동반된 기관기관지골 형성증 1예

허인경,박정배,성낙일,이영실,이영현,박성동,구관민,이종임,김성자 동국대학교 의학연구소 2006 東國醫學 Vol.13 No.1

기관기관지골 형성증(Tracheobronchopathia Osteoplastica, TPO)은 기관과 기관지내로 돌출된 다발성 연골성 혹은 골성 점막하 소결절들을 특징으로 하는 드문 양성질환이다. 기관기관지골 형성증의 원인은 아직 잘 알려져 있지 않다. 저자들은 기관지 결핵과 동반된 기관기관지골 형성증으로 확진된 예를 경험하였기에 이에 문헌고찰과 함께 보고하는 바이다. 환자는 67세 여자로 10일간의 마른 기침을 주소로 본원을 방문하였다. 발열이나 객담은 없었다. 흉부 단순 촬영 소견상 우중엽에 무기폐 소견을 보였으며 기관지 내시경 소견상 기관 전벽과 측벽에 다발성의 조약돌 모양의 소결절이 관찰되었고 생검을 시행한 결과 점막하에 성숙된 골조직이 관찰되었다. 기관지 세척물에서 나간 도말 검사상 항산성 염색 양성으로 확인되어 기관지결핵으로 진단하고 항결핵제 복용을 시작하였다. 치료후 기관지 결핵의 경과는 임상적, 방사선학적으로 호전을 보였다. 상기 환자에서와 같이 기관지 결핵과 기관기관지골 형성증이 동반된 예를 드물게 볼 수 있으나 아직 그 인과 관계는 명확하지 않다. Tracheobronchopathia Osteoplastica (TPO) is a rare, benign disease that is characterized by numerous osseous and cartilaginous submucosal nodules projecting into the lumen of the trachea and bronchi. The etiology of TPO remains unclear. We experienced a case of TPO associated with endobronchial tuberculosis in a 67 year-old female. She had been complained of dry cough for 10 days without sputum and fever. She showed radiologically atelectasis in the right middle lung field. Fibrotic bronchoscopy revealed numerous cobble-stone appearance nodules on the anterior and lateral wall of trachea. Pathologic examination of this lesion showed matured bone in the submucosa of trachea. As bronchial aspiration study indicated positive AFB stain, she was diagnosed as endobronchial tuberculosis. Antituberculosis medication has been started and resulted in clinical and radiologic improvement. Although it is rare, TPO may be associated with endobronchial tuberculosis as seen in this case. The relation may of cause and effect is, however, still unclear.

Poster Session 2 : Protein Structure and Function Signal Transduction ; The Direct Interaction of Phospholipase C-g1 with Phospholipase D2 Is Important for EGF Signaling

( Il Ho Jang ),( Suk Mook Lee ),( Jong Bae Park ),( Jong Hyun Kim ),( Chang Sup Lee ),( Eun Mi Hur ),( Kyong Tai Kim ),( Hitoshi Yagisawa ),( Pann Ghill Suh ),( Sung Ho Ryu ) 한국생화학분자생물학회 (구 한국생화학회) 2002 추계학술대회집 Vol.2002 No.-