커뮤니티공간으로서의 어린이공원 조성에 관한 연구

김연금,김성주,박환지,유다희,최성용,홍승모,Kim, Yun-Geum,Kim, Seong-Joo,Park, Hwan-Ji,Yu, Da-Hee,Choi, Sung-Young,Hong, Seung-Mo 한국조경학회 2008 韓國造景學會誌 Vol.35 No.6

This study deals with the participatory design process of renovating a children's park. The process should proceed with two major elements. Firstly, the children's park should be designed from the perspective of children. Secondly, it should be designed in consideration of the context of the community. This study is divided into two parts. First is the review of theory concerning the meaning and roles of the children's park as a community space, the importance of children's participation in the design and the participatory method of the children. The second is the case study. The site for the case study is Ssial Park in Nowon-Gu, Seoul. The entire process of the case study is with community participation, from identifying the park's problems to the actual construction. At every step, through the participatory programs, the community participates in the decision making. In addition, mutual understanding among participants is pursued through diverse means of communication. The following results of the case study were found. Firstly, diverse participatory programs and the active trial to enable the community to communicate contribute to the sense of ownership and responsibility concerning the park Secondly, the community can negotiate the differences in opinions without needing the help of experts. Of course, there are limits in the case study, such as the fact that the community organization, which is the core of communication and maintenance concerning the park, is not organized through the process of a case study. Another is that more diverse methods, which inform all community members of the participatory process, should have been used.

균류 분화과정의 유전적 조절기작

박범찬,이환희,박윤희,박희문 충남대학교 생물공학연구소 2003 생물공학연구지 Vol.9 No.1

Fungi are well suited for studying mechanism controlling development and cell differentiation in multicellular eukaryotes. The asexual and sexual reproductive cycles are tightly coordinated during the life cycle of Aspergillus nidulans. The asexual (mitotic) reproductive cycle involves the formation of a number of differentiated cell types. Successful formation of multicellular reproductive structures and spores (conidia) requires the establishment of spatiotemporal gradients in essential transcriptional regulators, such as brlA, abaA and wetA. Altered gene expression is coordinated with critical changes in the movement of nuclei and the regulation of the cell division cycle. Therefore, it has been proposed to define a central regulatory pathway. Several early regulatory genes involved in signal transduction pathway controlling asexual sporulation are also required for activating the central pathway. Sexual (meiotic) reproduction is more complex, requiring the differentiation of several specialized tissue types. Although technical difficulties have limited efforts to study the genetics of sexual fruiting-body formation directly, some recent progress has been made. With the recent availability of mutants blocked in sexual development, understanding the genetic interactions between genes that function primarily in determining sexual sporulation will be achieved in the near future.

프리미돈의 한국인에서의 생체이용율시험

권광일,윤민혁,윤희열,박희찬,권준택,심희옥,김동출 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

A reversed phase HPLC method was developed and validated for the determination of primidone in human plasma. Propylthiouracil was used as an internal standard. Calibration curves were linear in the concentration range of 10-120 ng/㎖. The coefficient of variation of the intra- and inter-day precision were below 15%. The coefficient of variation of the accuracy were below 15% in the concentration range investigated. A bioavailability study was performed using the validated HPLC method. Twenty four healthy human male volunteers were orally administered 20 mg of primidone. The pharmacokinetic parameters were calculated using WinNonlin. The mean values of AUC_(LAST) was 85.0±17.7 ㎍ hr/㎖, C_(max) was 4.22±1.04 ㎍/㎖, T_(max) was 1.85±1.13 hr, t_(1/2) was 17.7±2.4 hr. The pharmacokinetic parameters and the HPLC method can be used for the desigm of bioequivalence study of primidone.

Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.

Chung, Ji-Yun,Park, Hee Ra,Lee, Su-Jin,Lee, Sun-Hye,Kim, Jin Sik,Jung, Youn-Sang,Hwang, Sang Hyun,Ha, Nam-Chul,Seol, Won-Gi,Lee, Jaewon,Park, Bum-Joon United States and Canadian Academy of Pathology [e 2013 Laboratory investigation Vol.93 No.6

<P>Parkinson's disease (PD) is the second leading neurodegenerative disease, and is known to be induced by environmental factors or genetic mutations. Among the verified genetic mutations of PD, Parkin, isolated from the PARK2 locus, shows an autosomal recessive inheritance pattern and is known to be an E3 ligase. However, the physiological target of Parkin and the molecular mechanism of Parkin-deficiency-induced PD have not been clearly demonstrated until now. It has recently been proposed that inflammation, suggesting as a causal factor for PD, is enhanced by Parkin deficiency. Thus, we examined the relationship between inflammation-related factors and Parkin. Here, we provide the evidence that Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6 (TNF-α receptor-associated factor 2/6). Overexpression of Parkin can reduce the half-lives of TRAF2 and TRAF6, whereas si-Parkin can extend them. However, mutant Parkins did not alter the expression of TRAF2/6. Thus, loss of Parkin enhances sensitivity to TNF-α- or IL-1β-induced JNK activation and NF-κB activation. Indeed, si-Parkin-induced apoptosis is suppressed by the knockdown of TRAF6 or TRAF2. We also observed elevated expression levels of TRAF6 and a reduction of IκB in an 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model. Moreover, elevated expression levels or aggregation of TRAF6 were detected in approximately half of the human PD tissues (7/15 cases) and 2 cases, respectively. In addition, TRAF6 and Parkin expression levels show a reverse relationship in human PD tissues. Our results strongly suggest that the reduction of Parkin or overexpression of TRAF2/6 by chronic inflammation would be the reason for occurrence of PD.</P>

특수 언어발달 장애 환아의 임상 및음향음성학적 특성연구

김연희,박세훈,신용일,김찬양,김현기,김정수 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.1

數種의 腐熟劑 添加가 볏짚의 堆肥化에 미치는 影響

尹奉基,金炳浩,金喜權,朴仁珍 목포대학교 자연자원개발연구소 2000 자연자원연구 Vol.3 No.-

This study was conducted to find out effect of microorganisms added for decomposition of organic matter when manufacturing compost. The microorganisms used were EM, LACTO, SEMIP, BAIMUD circulated in market and 068065 developed in Institute of Agricultural Sciences. Effect of microorganisms inoulation on rice straw was summarized as follows: The number of bacteria, actinomycetes and fungi was 28.7∼76.7(×106), 0∼72.0(×106) and 0∼198.7(×105)cfu/g respectively. The number of bacteria, actinomycetes and fungi between microorganisms was no different. The maximum temperature during composting periods was reached 69∼76℃- at 3 50 5 days after heaping and kept over 65℃ for 7 to 10 days. The number of bacteria, actinomycetes and fungi in compost manufactured with rice straw was 42.8∼206.0(×106), 11.3∼54.7(×106), and 9.8∼42.5(×105)cfu/g respectively. The tendency was similar to control. Degree of decomposition of plot added microorganisms except 068065 and BAIMUD was similar to control at 50 days after heaping. The contents of inorganic elements were 1.26∼1.35% T-N, 1.14∼1.38% P2O5, 0.72∼0.97% K2O, 1.01∼1.74 CaO and 0.63∼1.44% MgO. Contents of T-N, P2O5 , CaO, MgO in compost were increased, but content of K2O was decreased, It was no different between treatments.

용액 중합에 의한 전도성 Poly(amide)/Polypyrrole 복합체의 제조와 그 전기적 성질

박연흠,안찬호,한명희 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.1

Poly(amide)/Polypyrrole composite films were prepared by solution ploymerization method. The films obtained by this method exhibited high conductivities. After polymerization of pyrrole for 1 hr, composite films showed the conductivity as high as 10^-3∼10^-2 S/cm and it increased with increasing the concentration of oxidizing agent. The stability of conductivity to ambient air exposure was excellent. As the result of the thermal characterization of composite films by TGA, we found that the composite film was thermally stable.

남부 중간지 자운영재배답에서 벼재배시 논 유형에 따른 질소시비량 구명

윤봉기,김병호,김희권,이인,최형국,박인진 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.1

중간지의 보통답과 사질답에서 녹비작물인 자운영을 재배한 논에 벼를 재배할 때 답 유형에 따른 질소비료 시용량을 구명하고자 전남 구례의 보통답과 사질답에서 관행, 자운영+질소 70%, 자운영+질소 50%, 자운영+질소 30%, 자운영+무질소의 5처리로 2000년부터 2년동안 시험한 결과는 다음과 같다. 1. 자운영의 개화성기 생육은 초장 44.1∼44.2㎝, 생초중 20.1∼22.5Mg ㏊^-1이였으며 무기성분 함량은 T-N 2.46∼2.68%, P_2O_5 0.56∼0.60%, K_2O 2.05∼2.18%였다. 사질답에 비하여 보통답에서 자운영의 생초중이 많았다. 2. 토양중 NH_4-N함량의 경시적 변화는 이앙 후 7일에 21.5∼48.3㎎ ㎏^-1으로 가장 높았으며 이앙후 70일에 7.0∼9.7㎎ ㎏^-1으로 점차 감소하였는데 처리에 따라서는 자운영+질소 70%, 자운영+질소 50%시용구에서 높았다. 3. 벼 수확기의 간장 76∼85㎝, 수장 17.9∼19.2㎝로 사질답이 보통답에 비하여 간장, 수장이 컸다. 4. 시험후 토양의 화학성은 pH 5.4∼5.8, OM 21.5∼24.4g ㎏^-1, CEC 9.2∼10.9cmol ㎏^-1, Ava. P_2O_5 141∼360㎎ ㎏^-1으로 자운영처리구에서 OM, CEC함량이 증가하였다. 5. 벼의 수량은 관행(보통답 5.03Mg ㏊^-1, 사질답 5.18Mg ㏊^-1)에 비하여 보통답의 자운영+질소 70%, 50%시용구에서 각각 5, 4%증수되었고, 사질답에서는 자운영+질소 70%, 50%시용구에서 각각 7, 4%증수되었다. 6. 남부 중간지에서 자운영을 재배하여 생초 20Mg ㏊^-1를 녹비로 투입하면 벼를 재배시 질소 시용 적정량은 보통답, 사질답 모두 관행 시비량의 50%이였다. This study was conducted to find out fertilizer recommendation rates for rice at a normal and sandy paddy field after planting milk vetch(MV) in Guryae which lies to southern medium altitude region from 100m to 250m above sea level from 2000 to 2001. The amount of N fertilizer were 110(normal paddy) and 130(sandy paddy) ㎏ ㏊^-1 at control and 0, 30, 50, 70% of the control. At flowering stage of milk vetch, the content of T-N, P_2O_5, K_2O in plant were 2.46∼2.68%, 0.56∼0.60%, 2.05∼2.18%, respectively. Plant height were 44.1∼44.2㎝. Fresh weight were 20.1∼22.5Mg ㏊^-1. Fresh weight of milk vetch cultivated in normal paddy field was more than that in sandy paddy field. The content of NH_4-N in soil was 21.5∼48.3㎎ ㎏^-1 at 7 days after planting and 7.0∼9.7㎎ ㎏^-1 at 70 days after planting. Content of NH_4-N in soil was increased at the treatments of milk vetch + N 70, 50% of control. Culm length was 76∼85㎝, panicle length was 17.9∼19.1㎝ at harvesting stage. Culm length and panicle length at sandy paddy field were longer than those of normal paddy field. Chemical properties of soil after experiment were pH 5.4∼5.8, OM 15.6∼21.5g ㎏^-1, CEC 9.2∼10.9cmol ㎏^-1, Ava. P_2O_5 141∼360㎎㎏^-1, respectively. The content of OM, CEC in soil applied milk vetch at normal paddy field was higher than those of the control, and the content of CEC in soil applied milk vetch at sandy paddy field was higher than that of the control. When milk vetch was cut at flowing stage, rice yield of milk vetch + N 70, 50% of control plot were increased 5, 4% more than that of control(5.03Mg ㏊^-1) at normal paddy field. Rice yield vetch+N 70, 50 of control were increased 7, 4% more than that of control(5.18Mg ㏊^-1) at sandy paddy field. Nitrogen application rate of normal and sandy paddy field was 50% of conventional fertilization rate when 20Mg ㏊^-1 milk vetch cultivated paddy field was inputed for rice culture in the southern medium altitude.

Apolipoprotein E 다형성에 따른 사업장 근로자의 혈중 지질농도, 영양소 섭취 및 건강관련 생활습관

박유경,조상운,강지연,백윤미,성숙희,최태인 대한지역사회영양학회 2008 대한지역사회영양학회지 Vol.13 No.5

The purpose of this study was to investigate the association among nutrient intakes and health-related lifestyles with cardiovascular disease risk assessed by blood lipid profile according to Apolipoprotein E genotypes. Middle-aged industrial male workers who had completed their annual medical examination were recruited and data of 675 subjects who finished the nutrient survey were used in the analysis. Anthropometric parameters, dietary assessment (FFQ), health-related lifestyles and blood profiles were used for statistical analyses. Apo E genotype groups were classified into the following three genotypes: Apo E2 group (including E2/E2, E2/E3, E2/E4), Apo E3 group (including E3/E3), Apo E4 group (including E3/E4, E4/E4). The frequency of Apo E2, E3, and E4 allele were 13.3%, 75.0% and 11.7% respectively. There were no significant differences in the anthropometric parameters depending on different Apo E genotypes. Also, no significant differences in the nutrient intakes were found according to the genotype groups. The nutrient intakes of all subjects were similar to or higher than the level of KDRIs (Dietary Reference Intakes For Koreans) except for intakes of calcium (67.44% of KDRIs), vitamin A (73.83% of KDRIs) and vitamin B2 (78.02% of KDRIs). Also, there were no significant differences of health-related lifestyles according to Apo E genotype groups. As for the lipid profiles, Apo E4 group had significantly higher total and LDL-cholesterol concentrations than the Apo E2 group (p < 0.05). We confirmed that plasma total and LDL-cholesterol concentrations were greatly influenced by Apo E genotypes. However, nutrient intakes and health-related lifestyles were not associated with Apo E genotypes.

로자탄 대 포시노프릴의 고혈압 환자들에서 항고혈압 효과, 안전성 및 내약성의 비교

박대균,이명묵,채인호,이무용,이홍자,김효수,김철호,손대원,오병희,박영배,최윤식 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.1