무증상의 현미경적 혈뇨를 동반한 성인 환자에서 신조직 검 사의 유용성

김형종,최훈영,김동기,김현진,장제현,김현욱,최규헌,이호영,한대석,강신욱 대한신장학회 2003 대한신장학회잡지 Vol.22 No.6

국가예방접종전산등록체계 개발을 위한 민간의료 기관 하부구조 및 환경 조사연구

이무식,김은영,이건세,이석구,홍지영,김건엽,김대경,신의철,이연경,기몽서,나백주,김영택 한국모자보건학회 2003 한국모자보건학회지 Vol.7 No.2

형광검출 역상 액체크로마토그래피에 의한 Histamine의 정량

유희춘,김형룡,김상현,김대기,이영미,김형민,안년형,신태용 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Histamine을 정확하고 신속하게 정량하기 위해 9.Fiuorenylmethyl chloroformate를 형광유도체화제로 하여 역상 HPLC법으로 정량하였다. 히스타민을 형광유도체화할 때 반응액의 pH, 반응시간, 형광유도체화제의 농도 등 최적 반응 조건을 검토하였다. 이 방법으로 히스타민을 분석한 결과 0.1~0.5ug/ml의 농도범위에서 상관계수가 0.922인 양호한 직선성을 나타내었으며 검출한계는 0.01??/ml였다. A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatiation such as pH, reaction time and he concentration of FMOC were investigated. Linearity of calibration curve was obtained between 0.1ug/ml/and 0.5ug/ml(r=0.922) and the limit of detection was 0.01ug/ml.

비만증 환자에서 한약제에 의한 체중 감소효과

Oh, Seung-Joon,Jeong, In-Kyung,Kim, Young-Seol,Choi, Young-Kil,Paeng, Jeong-Ryung,Bae, Jung-Hwan,Shin, Hyun-Dae 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Seung-Joon Oh, In-Kyung Jeong, Young-Seol Kim, Young-Kil Choi, Jeong-Ryung Paeng¹, Jung-Hwan Bae and Hyun-Dae Shin²Department of internal Medicine, College Medicine, Endocrine Research Institute¹. Department of Rehabilitation, College of Oriental Medicine², Seoul, korea. Bady Fat Reduction Effects of Red Ginseng Compound Preparation on the Patients with Obesity. Proceedings of International Symposium on East-West Medicine, Seoul. 244-254, 1999.-Obesity can be defined as a metabolic disease due to an increased state of fat tissues caused by an imbalance of calorie intake and use. Recently, in Korea by improvement and westernization of food intake, along with decrease in exercise activities, the prevalence of obesity has increased greatly. Our objectives were to study stability and effects of decrease in body fat by administering red ginseng compound preparation (known to have body fat decreasing effects in laboratory animals) to obesity patients on low calorie diets. Changes in weight and body fat were measured while carrying out calorie-restricted diets on patients for 4 weeks, then administering red ginseng compound preparation for another 4 weeks. The patients were 20 people whose BMI were 25kg/㎡ or over and whose percent body fat was also 30% or over when tested by bioelectrical conductivity. 1. Changes in weight were from 70.04kg(base line) to 67.43kg(after taking red ginseng compound preparation). 2. In similar sense, BMI decreased from 27.12kg/㎡(base line) to 26.56kg/㎡(after dieting), and further to 26.01kg/㎡ (after taking red ginseng compound preparation). The BMI seemed to decrease significantly compared to the baseline after the use of red ginseng compound. 3. Waist hip ratio was changed from 0.8858(base line) to 0.8728, but it was statistically insignificant. 4. The percent body fat was 35,16%(base line), 33.87%(after dieting), and 31.68%(after taking red ginseng compound preparation). 5. Complete blood cell count and blood chemisrty remained unaffected by the administration. 6. In concern to endocrinologic studies, T3 decreased from 118.7 to 98.2ng/dL, and T4 increased from 8.8 to 9.2㎕/ dL. Epinephrine showed a tendency to decreased from 0.27 to 0.25 ng/mL, and norepinephrine increased from 0.39 to 0.44ng/mL. 7. Leptin was not changed. 8. Some patients complained adverse effects; constipation(5 patients, may be due to diet therapy), fatigue (2 patients), pruritus(2 patients), flushing(s patients), dizziness(3 patients) and epigastric discomfort(2 patients). However their symptoms were mild, so medication did not stopped. In conclusion, loss of weight without significant side effects was observed during low calorie diet and red ginseng compound preparation administration. This is thought to be in relation to sympathetic nerve system rather than adrenal gland. Also, further long0term studies should be required, since the observed results were based on short-term changes in weight.

아몰퍼스 자성박대의 자기센서 응용에 관한 연구

金大柱,林在根,金玄郁,愼鏞鎭 明知大學校 産業技術硏究所 2002 産業技術硏究所論文集 Vol.21 No.-

In this thesis, we have described about application of amorphous magnetic ribbon of FeCoSiB compound to magnetic sensing device. As the samples of our experiment, we used a near zero-magnetostrictive amorphous magnetic ribbon of (Fe0.06Co0.94)79Si2.1B18.9 composition with its thickness of 26[㎛], its length of 5[㎝] and its width of 2[㎜], which was fabricated by rapidly quenching method. In order to improve the magnetic properties of the samples, we annealed them under magnetic field where the range of the crystallization temperature Tx and the Curie temperature Tc were 300~400[℃]. Then, by measuring them with X-ray diffraction(XRD) and vibrating sample megnetometer(VSM) analysis method. we found Tx≒451[℃] and Tc≒441[℃]. From the hysteresis curve of the finally annealed sample ribbon, we found the Large Bakhausen Jump(LBJ) effect at the reversal magnetization field Hw≒0.5[Oe]. This LBJ effect was also observed at the variation of the magnetic domain in the process of magnetization. Thus, it is proved that the amorphous magnetic ribbon of FeCoSiB compound can be use as a magnetic sensing device.

승용차 완충장치의 고주파 소음전이 구조해석에 관한 연구

김대원,최영휴,신중호 國立 昌原大學校 産業技術硏究所 1995 産技硏論文集 Vol.9 No.-

Study on new and high technology as electronic control system is used in shock absorber is being carried out but good quality of shock absorber can not be developed without further study on the improvement of noise magnitude, friction resistance reduction, temperature characteristics, damping force scattler reduction which is basic performance in shock absorbers. This study was carried out to clarify transmitted structure, using experimental and theoretical method for high frequency transmitted noise which is one type of structural born noises in the shock absorbers and to analyze the relationships between the high frequency transmitted noise and the characteristics of each component in the passenger car shock absorbers.

Al_(0.3)Ga_(0.7)As/GaAs 다중 양자우물구조에서 Photolumunescence의 온도 의존성

김동렬,배인호,김대년,박성배,신영남 大邱大學校附設 基礎科學硏究所 1998 基礎科學硏究 Vol.15 No.1

Temperature dependent photoluminescence(PL) has been applied to characterize the subband transition in Al_(0.3)Ga_(0.7)As/GaAs multiple quantum wells grown by molecular beam epitaxy. The PL signal at 295 K showed main peak located at 1.4795 eV with a shoulder around 1.4973 eV. They are associated with the E1 H1 and E1 L1. From temperature dependent PL intensity, activation energy is determined to be 22 meV. From temperature dependent PL linewidth, γ_(i) and γ_(c) are determined to be 5.26±0.2 meV, 136±52 meV, respectively. When the lattice temperature was decreased, the peak energy increased, following the temperature dependence of the bulk GaAs band gap, down to around 30 K, and then deviated to the lower energy side by about 3.5 meV at 12 K.

燒却爐 洗淨集塵機의 排出水 處理

신대윤,홍완해,김형곤 조선대학교 환경공해연구소 1999 環境公害硏究 Vol.15 No.2

This study has some useful information about washout water from the small scale incinerator, installed in Chosun university. The results are as follows; 1. The concentration of SS has 65.2㎎/ℓ after being screened by micro strainer. 2. Alum(10% as Al_(2)O_(3) : 3㎖/ℓA-601P 4㎖/ℓ was added in pH 7.5 for coagulating screened washout water. The removal rate after carrying chemical coagulation turned out to be 85.8% in SS, 45.4% in CODMn, 64.5% in BOD with concentration 9.1㎎/ℓ ,93.5㎎/ℓ and 134.8㎎/ℓ respectively. Some heavy metals were also analysed and their removal efficiency was higher than expected as below : Pb, Zn, and Fe was determined as 0.76㎎/ℓ , l.06㎎/ℓ, 2.63㎎/ℓ with removal rate of 93.7%, 86.6% and 80.6% in each item, respectively. 3. The optimum HRT for sample which is mixed with coagulated washout water and screened sewage wastewater on HBC-briquet ash complicative media was 13.4hr. Mixed sample with different rate like 1 : 1(ratio of chemically coagulated water to sewage wastewater on a half and half bases) obtained from biomembrane reactor with 13.4hr retention time, was determined value as 35㎎/ℓ (38.6% removal rate), 28.3㎎/ℓ (81.3% removal rate) and 51.7㎎/ℓ (75.7% removal rate) with respect with SS, BOD and CODMn, respectively.

교맥 에탄올 추출물의 멜라닌생성 억제효과

김대성,노성택,이장천,임규상,신미란,우원홍,문연자 한국전통의학연구소 2006 한국전통의학지 Vol.15 No.1

The aim of this study was to investigate the effect of ethanol extract of Fagopyrum escuentum(FE) on the melanogenesis. To determine whether ethanol extract of FE suppress melanin synthesis in cellular level, B16F10 melanoma cells were cultured in the presence of different concentrations of FE ethanol extract. In the present study, the author examined the effects of FE ethanol extract on cell proliferation, melanin contents, tyrosinase activity. Cell proliferation was slightly increased by treatment with ethanol extract of FE (25-200 ㎍/ml). The ethanol extract of FE effectively suppressed melanin contents at a dose of 100 ㎍/ml. It was observed that the color of cell pellets was totally whitened compared with the control. The ethanol extract of FE inhibited tyrosinase activity, regulate melanin biosynthesis as the key enzyme in melanogenesis. These results suggest that the ethanol extract of FE exerts its depigmenting effects through the suppression of tyrosinase activity. And it may be a potent depigmetation agent in hyperpigmentation condition.

대뇌 기저핵 신경세포 일차배양에서 망간에 의해 유도된 Appoptosis : 형태학적인 소견

신동훈,김상표,정용욱,배재훈,송대규,백원기 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목적 : 본 실험은 대뇌기저핵의 신경세포를 배양하여 망간(MnCl₂)을 투여한 후 망간독성에 의한 신경세포의 apoptosis를 형태학적인 소견으로 관찰하였다. 방법 : 배양된 신경세포에 0.01에서 10μM Mnc12를 48시간동안 처리한 후 TUNEL (TdT-mediated dUTP Nick End Labelling)법 및 투과전자현미경학적으로 관찰하였다. 결과 : TUNEL방법을 이용하여 관찰한 결과 TUNEL반응에 갈색으로 양성반응을 나타내는apoptotic 세포의 수가 대조군에 비해 MnCl₂를 투여한 군에서 유의하게 높게 나타났으며(P〈0.05), 투과전자현미경학적 소견상 대조군의 신경세포들은 핵인(nucleolus)이 두드러지게 특징적으로 보이면서 핵막과 세포질내 소기관들이 잘 보존되어 있으며, 세포질내망(ER)과 사립체(mitochondria)를 특히 많이 가지고 있었다. Mnc12를 48시간 동안 처리한 군에서 이질염색질(heterochromatin)이 핵막으로 이동하면서 응집되어 있었으며, 핵내 불규칙한 형태의 염색질이 나타나 분절이 진행되는 소견을 보였고, Apoptosis의 가장 특징적인 초기 소견인 막으로 둘러싸인 반달모양의 핵내염색질의 분절편(fragment-ed chromatin)과 주위의 상대적으로 정상적인 소기관으로 구성된 apoptotic body를 관찰할 수 있었다. 결론 : 신경세포에서 망간에 의해 apoptosis가 유도됨을 형태학적인 방법으로 확인할 수 있었으며 망간에 의한 세포사망양상에 apoptosis가 하나의 기전이 될 수 있을 것이다. Objectives : Manganese is cytotoxic to the central nervous system including basal gan-glia. Its toxic mechanism is related to oxidative stress, mediated by toxic free radicals but is specultives. In the present study , we have investigated to manifest apoptosis in man-ganese-induced cytotoxicity in primary neuronal cell culture of rat basal ganglia. Methods : To detect apoptotic neuronal cells were stained by the terminal deoxynu-cleotide (TdT) -mediated dUTP nick end-labelling (TUNEL) method and apoptotic changesin nuclei of neurons were observed by electron microscopy. Results : We showed that TUNEL immunostain showed brownish signal in the nuclei of apoptotic cells and the proportions of apoptotic cells in Manganese treatment groups were more higher than controls. On transmission electron microscopy, there were chromatine condensation with margination toward nuclear membrane and condensation of cyto-plasm in the treated with luM MnCl²for 48 hours in a basal ganglia neurons. Apoptotic bodies were found and consisted of semilunar-like condensed nuclei with relatively intact cytoplasmic organelles. Conclusions : Apoptosis appears to be one mechanism in the manganese-induced net-ronal cell death. Manganese intoxication is a convenient model for apoptosis study.