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      • KCI등재

        Protein transduction of an antioxidant enzyme: subcellularlocalization of superoxide dismutase fusion protein in cells

        김대원,장상호,강태천,원무호,조성우,박진서,이길수,음원식,최수영,권오신,이선화,So Young Kim,Yeum Pyo Lee,Min Jung Lee,정민섭 한국생화학분자생물학회 2008 BMB Reports Vol.41 No.2

        In protein therapy, it is important for exogenous protein to be delivered into the target subcellular localization. To transduce a therapeutic protein into its specific subcellular localization, we synthesized nuclear localization signal (NLS) and membrane translocation sequence signal (MTS) peptides and produced a genetic in-frame SOD fusion protein. The purified SOD fusion proteins were efficiently transduced into mammalian cells with enzymatic activities. Immunofluorescence and Western blot analysis revealed that the SOD fusion proteins successfully transduced into the nucleus and the cytosol in the cells. The viability of cells treated with paraquat was markedly increased by the transduced fusion proteins. Thus, our results suggest that these peptides should be useful for targeting the specific localization of therapeutic proteins in various human diseases.

      • KCI등재후보

        EST Knowledge Integrated Systems (EKIS): An Integrated Database of EST Information for Research Application

        김대원,정태성,최영상,권혁렬,김동욱,최한석,최상행,박홍석 한국유전체학회 2009 Genomics & informatics Vol.7 No.1

        The EST Knowledge Integrated System, EKIS (http://ekis. kribb.re.kr), was established as a part of Korea's Ministry of Education, Science and Technology initiative for genome sequencing and application research of the biological model organisms (GEAR) project. The goals of the EKIS are to collect EST information from GEAR projects and make an integrated database to provide transcriptomic and metabolomic information for biological scientists. The EKIS constitutes five independent categories and several retrieval systems in each category for incorporating massive EST data from high-throughput sequencing of 65 different species. Through the EKIS database, scientists can freely access information including BLAST functional annotation as well as Genechip and pathway information for KEGG. By integrating complex data into a framework of existing EST knowledge information, the EKIS provides new insights into specialized metabolic pathway information for an applied industrial material.

      • KCI등재후보

        DNA Barcoding of Fish, Insects, and Shellfish in Korea

        김대원,백운기,임정희,유원기,박현철,유혜숙,강동원,진선덕,민홍기 한국유전체학회 2012 Genomics & informatics Vol.10 No.3

        DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a DNA bio-barcode. We collected DNA barcodes, based on COI sequences from 156 species (529 sequences) of fish, insects, and shellfish. We present results on phylogenetic relationships to assess biodiversity the in the Korean peninsula. Average GC% contents of the 68 fish species (46.9%), the 59shellfish species (38.0%), and the 29 insect species (33.2%) are reported. Using the Kimura 2 parameter in all possible pairwise comparisons, the average interspecific distances were compared with the average intraspecific distances in fish (3.22 vs. 0.41), insects (2.06 vs. 0.25), and shellfish (3.58 vs. 0.14). Our results confirm that distance-based DNA barcoding provides sufficient information to identify and delineate fish, insect, and shellfish species by means of all possible pairwise comparisons. These results also confirm that the development of an effective molecular barcode identification system is possible. All DNA barcode sequences collected from our study will be useful for the interpretation of species-level identification and community-level patterns in fish, insects, and shellfish in Korea, although at the species level, the rate of correct identification in a diversified environment might be low.

      • KCI등재

        바렐도금용 폐Steel Ball로부터 산화주석 및 금속주석 회수에 관한 연구

        김대원,장성태 한국세라믹학회 2012 한국세라믹학회지 Vol.49 No.6

        A study of the recovery of tin and nickel from steel ball scraps for barrel plating was carried out through a physical treatment, a leaching treatment, hydrogen reduction and an electrolysis experiment. The recovery of the iron component was over 95% by the physical treatment. We obtained tin oxide in the form of metastannic acid (SnO2·xH2O) with impurities of less than 5% from the leaching treatment. We also recovered the high-purity metallurgical tin at a rate that exceeded 99.9% by the electrolysis of crude tin obtained from the hydrogen reduction of metastannic acid.

      • KCI등재

        Bubget Watch and Finance Democracy- Focus on the analysis of citizens' attitude about the budget watch in budgetary process -

        김대원 한국정부학회 2004 한국행정논집 Vol.16 No.1

        본 연구는 재정민주주의의 구현을 위해서는 외부감시자로서의 국민들의 역할과 관심이 높아야 한다는 전제하에 구조방정식모형을 이용하여 예산몰입과 재정민주주의에 영향을 미치는 구조요인을 분석하는 데 목적을 두었다. 실증 연구를 위해 이론적 논의에 근거하여 추출된 가설을 설정하고, 실증자료를 이용하여 분석하였다. 분석 및 검증을 위해 요인분석, 구조방정식모형(structural equation modeling) 등의 통계기법을 이용하였다. 분석결과, 진정한 재정민주주의를 구현하기 위해서는 기존의 예산감시 기관만으로는 한계가 있기 때문에 국민들의 적극적인 참여를 전제로 하는 예산감시 기구의 중요성이 확인되었다.

      • Order Imbalance, Stock Return, and Financial Restatement

        김대원 계명대학교 산업경영연구소 2015 經營經濟 Vol.48 No.1

        Purpose - In this paper, we conduct a out-of-sample test of Chordia and Subrahmanyam (2004) using the sample of 623 NYSE companies that made restatement on their previous financial results for the period of nine years (Jan, 1997 to Sept, 2005). Design/Methodology - Using transaction data of the sample, we estimate two measures of order imbalance. To test the effects of lagged imbalances on return, we use time-series regression. Employing an event-study methodolgy, we also analyze the movement of trading activity, the imbalance measures, and return in the event period of restatement announcement. Findings - The autocorrelation in order imbalance reduces substantially in our study period compared to their research period. In general, our results corroborate the predictions of CS's theory: a positive relation between the current return and the lagged imbalances and the reversal of the effect of the lagged imbalance in presence of the contemporaneous imbalance. But our results indicate that due to the weakened autocorrelation the effect of the lagged imbalances on the return diminish. Still the contemporaneous imbalance exerts significant influence on the contemporaneous return. Implications - In the framework of an event study, we find excessive selling pressure with before the event day, which is considered informed trading. Examining the relation between changes in order imbalances and changes in return, we find that the effect of the lagged imbalances become negligible. Only the change in the contemporaneous imbalance is significantly and strongly associated with the change in the contemporaneous return.

      • KCI등재

        Estimation of Hydrodynamic Coefficients from Sea Trials Using a System Identification Method

        김대원,Knud Benedict,Mathias Paschen 해양환경안전학회 2017 海洋環境安全學會誌 Vol.23 No.3

        This paper validates a system identification method using mathematical optimization using sea trial measurement data as a benchmark. A fast time simulation tool, SIMOPT, and a Rheinmetall Defence mathematical model have been adopted to conduct initial hydrodynamic coefficient estimation and simulate ship modelling. Calibration for the environmental effect of sea trial measurement and sensitivity analysis have been carried out to enable a simple and efficient optimization process. The optimization process consists of three steps, and each step controls different coefficients according to the corresponding manoeuvre. Optimization result of Step 1, an optimization for coefficient on x-axis, was similar compared to values applying an empirical regression formulae by Clarke and Norrbin, which is used for SIMOPT. Results of Steps 2 and 3, which are for linear coefficients and nonlinear coefficients, respectively, was differ from the calculation results of the method by Clarke and Norrbin. A comparison for ship trajectory of simulation results from the benchmark and optimization results indicated that the suggested stepwise optimization method enables a coefficient tuning in a mathematical way.

      • KCI등재

        Analysis of - inefficiency in Local Government Nonparametric Approach

        김대원 한국정부학회 2004 한국행정논집 Vol.16 No.4

        지방정부의 효율성을 높이기 위해서는 지방정부의 효율성을 정확하게 측정할 수 있어야 한다는 전제가 필요하다. 본 연구는 지방정부의 효율성을 측정하기 위한 유용한 방안으로 평가되고 있는 DEA를 우리나라 지방정부의 효율성 평가에 적용하였다. 경험적 분석결과에 의하면 이 분석방법의 효용성은 충분히 큰 것으로 나타났다. 비록 30개의 분석 단위를 사용하였다는 한계는 있지만 유사한 규모의 다른 지방정부의 효율성 평가에도 의미있게 적용될 수 있을 것으로 판단된다.

      • KCI등재

        규칙적인 진동 하에서 노즐 부착된 제트화염의 연소특성

        김대원,이기만 한국화재소방학회 2009 한국화재소방학회논문지 Vol.23 No.1

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        A general combustion characteristics of forcing nonpremixed jet in laminar flow rates have been conducted experimentally to investigate the effect of forcing amplitude with the resonant frequency of fuel tube. There are two patterns of the flame lift-off feature according to the velocity increasing; one has the decreasing values of forcing amplitude on the lift-off occurrence when a fuel exit velocity is increasing, while the other has the increasing values. These mean that there are the different mechanisms in the lift-off stability of forced jet diffusion flame. Especially, the characteristics of attached jet flame regime are concentrically observed with flame lengths, shapes, flow response and velocity profiles at the nozzle exit as the central figure. The notable observations are that the flame enlogation, in-burning flame and the occurrence of a vortical motion turnabout have happened according to the increase of forcing amplitude. It is understood by the velocity measurements and visualization methods that these phenomena have been relevance to an entrainment of surrounding oxygen into the fuel nozzle as the negative part of the fluctuating velocity has begun at the inner part of the fuel nozzle. 층류영역의 분출유량에서 큰 가진강도 효과를 얻기 위해 연료관 관 공명주파수로 가진된 비예혼합 분류 화염의 일반적인 가진 연소특성을 실험적으로 조사하였다. 화염 안정화 특성에서는 두 가지 형태의 부상 특성이 존재하는 사실을 알았는데, 화염이 부상되는 가진강도 크기에서 한 쪽은 감소, 다른 영역에서는 증 가하는 것으로 나타나 각각 서로 다른 부상기구가 존재함을 확인할 수 있었다. 특히 부상되지 않고 노즐 에 부착된 분출유량 영역에서의 가진 연소특성을 가진강도에 따른 화염 길이와 형상, 유동장 응답 특성 그리고 노즐 출구에서의 유속 분포를 중심으로 집중 조사하였다. 특이한 현상으로는 가진 강도 증가에 따 라 화염의 신장과 in-burning 현상 그리고 화염 내 거동 와동들의 말림방향이 서로 역전되는 현상 등이 발견되었다. 노즐 출구의 유속분포와 가시화 기법을 통해 이러한 현상들이 노즐관 관벽 안쪽서부터 음의 속도가 발생하기 시작함에 따라 주변 산화제인 공기가 노즐관 안으로 유입되는 현상과 관련되는 것으로 파악되었다.

      • KCI등재
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