도토리 추출물의 3T3-L1 세포 분화억제 효과

김지연,이진,이창원,김애정,Kim, Ji-Yeon,Lee, Jin,Lee, Chang-Won,Kim, Ae-Jung 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

본 연구에서는 만성질환의 주요 위험인자인 비만을 예방하기 위한 식품소재로서 3T3-L1세포를 이용하여 탈피한 도토리의 항비만 효과를 알아보고자 하였다. 3T3-L1 세포에서 생존율(MTT assay)을 측정한 결과, AE와 AW 시료 모두 $500{\mu}g/mL$ 농도에서는 다소 생존율의 감소를 보여, $300{\mu}g/mL$를 최종 농도로 정하였다. 3T3-L1 세포의 지질축적 억제 효과를 측정한 결과, 농도 $100{\mu}g/mL$로 처리하였을 때 두 시료 모두 지질축적량의 증가를 보였으나, $200{\mu}g/mL$ 처리농도에서 AE 시료는 82%로, AW 시료는 74%로 감소되다가 $300 {\mu}g/mL$ 농도에서는 두 시료 모두 약 53% 수준까지 지질축적이 억제되었다. 3T3-L1 세포에서 중성지방 억제 효과를 확인한 결과, AE 시료의 경우 $200{\mu}g/mL$ 농도에서 11%의 감소율, $300{\mu}g/mL$ 농도에서 42% 수준의 감소율을 보였다. AW 시료도 $200{\mu}g/mL$ 농도에서 5%의 감소율과 $300{\mu}g/mL$에서 41%의 감소율을 보였다. 3T3-L1 세포의 ROS 생성량을 측정한 결과, 시료 농도 $200{\mu}g/mL$에서 AE는 42%, AW는 33%로 $300{\mu}g/mL$에서는 AE는 58%, AW는 52%로 ROS 생성량의 억제를 보였다. 3T3-L1 세포에서 mRNA 발현에 미치는 영향을 대조군과 비교하였을 때 두 시료(AE와 AW) 모두 $300{\mu}g/mL$ 농도에서 $PPAR-{\gamma}$은 54%와 38%, aP2는 40%와 18% 수준의 발현을 억제시키는 것으로 나타났다. 이상의 결과로 볼 때 탈피한 도토리는 3T3-L1 세포의 분화를 억제함으로써 새로운 항비만 소재로의 가능성이 있는 것으로 판단된다. This study aimed to investigate the suppressive effect of acorn extracts, by evaluating 70% ethanol extract (AE) and hot water extract (AW) using 3T3-L1 preadipocytes. We applied various levels (0, 100, 200, 300 and $500 {\mu}g/mL$) of AE and AW to 3T3-L1 preadipocytes. The cell viability of the 3T3-L1 preadipocytes was not affected by up $300 {\mu}g/mL$ of extracts, but was suppressed by level $500{\mu}g/mL$ of both AE and AW by 20% and 9% respectively. The accumulation of lipid droplets in differentiated 3T3-L1 preadipocytes was dose-dependently suppressed by AE and AW. Especially, at high concentrations ($300{\mu}g/mL$), AE (42%) was more effective than AW (41%). Reactive oxygen species (ROS) was also dose-dependently suppressed by treatment with AE (58%) and AW (52%). With regard to the mRNA related to differentiated 3T3-L1 preadipocytes, $PPAR-{\gamma}$ and aP2 were suppressed by treatment with AE (54 and 40%) and AW (38 and 18%). From our results, acorn extract (AE) has more suppressive effects than AW in differentiated 3T3-L1 preadipocytes. We therefore concluded that acorn has suppressive effects against obesity in differentiated 3T3-L1 cells due to antioxidation.

Acute exacerbation of interstitial lung disease; subgroup analysis for causes, risk factors for mortality rate, and steroid responsiveness

( Seung Hyun Yong ),( Ah Young Leem ),( Song Yee Kim ),( Kyung Soo Chung ),( Ji Ye Jung ),( Young Ae Kang ),( Young Sam Kim ),( Joon Chang ),( Moo Suk Park ),( Hye Jin Jang ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-

Background: Interstitial Lung Disease (ILD) has known as a poor prognosis disease, particularly idiopathic pulmonary fibrosis (IPF). Corticosteroid is widely used for treatment of acute exacerbation (AE)-ILD. This study aims to clarify efficacy of corticosteroid use, and compare the mortality rate depending on the subgroups of ILD. Materials and Methods: We collected the data from the patients who have diagnosed ILD and visited emergency room (ER) with the respiratory symptoms from January 1, 2016 to December 31, 2018. We classified AE-ILD patients into 3 groups depending on methylprednisolone dose per kilogram. Also, we compared the mortality of IPF patients to that of non-IPF patients. Results: A total case of AE-ILD visited ER from 2016 to 2018 was 209 cases, including IPF cases (n=122) and non-IPF cases (n=87). We divided total patients (n=209) into three groups depending on steroid use, and these include group 1 of 0-0.49 mg/kg (n=78), group 2 of 0.5-0.99 mg/kg (n=58), and above 1.0 mg/kg (n=41), each. We found poor prognosis from the cases of IPF compared to non-IPF cases (p=0.029, OR 2.463). Furthermore, there was significant differences of mortality rate according to steroid dose (p=0.036, OR 1.712), CRP elevation (p=0.016, OR 1.004), and intubated case (p=0.000, OR 8.152). Also, we categorized IPF (n=122) to non-IPF cases (n=87) and analyzed the mortality rate. Most of the patients were death from ILD itself (n=23, 63.9%), Pneumonia(n=8, 22.2%), and Lung cancer (n=5, 13.9%). Bacterial infection (n=26) was dominant cause of AE, followed by fungal infection (n=15), and viral infection (n=10). Conclusions: This retrospective analysis of corticosteroid treatment shows more fatal outcome when we use high dose steroid in AE-ILD cases, and there was significant difference in mortality rate depending on the subgroup of ILD and medical background, such as intubation status, higher CRP level, and IPF cases.

과학적 근거 중심 평가를 도입한 마늘의 콜레스테롤 조절 기능성 평가

김인혜;조강진;김진영;황경아;황유진;채유진;엄애선 한양대학교 2010 韓國 生活 科學 硏究 Vol.30 No.1

Garlic (Allium sativum) is used a flavouring agent and a traditional medicine in human history. Numerous studies support the fact that garlic intake may have effect on cholesetrol-lowering. No claims currently have been made about garlic intake and cholesterol-lowering effect with respect to health claim in Korea. The aim of this study was to evaluate the scientific evidence on garlic intake and cholesterol-lowering effect by the scientific evidence-based rating system of Korean Food and Drug Administration for health claim. We carried out a comprehensive search of the Pubmed, EMBASE, Cochrane Library and manual searches of relevant journals for studies between 1955-2009. The search terms were garlic and Allium sativum in combination with cholesterol. Eighteen clinical stidies were included and reviewed to evaluate the strength of evidence that supports a relation between garlic intake and cholesterol-lowering effect. Our results suggests that level of scientific evidence on garlic intake and cholesterol-lowering effect is "other function (II) probable".

간호 학생의 임상실습 효율을 위한 기초 조사 연구

이애경,김정애,주미경,정안순,배경진,김정수,이정애,장은정 경복대학 2000 京福論叢 Vol.4 No.-

임상실습 교육은 간호이론을 실무에 적용하는 기회를 제공하는 과정으로서 임상실습이 간호교육의 중요한비중을 차지하고 있다는 것은 주지의 사실이다. 이러한 중요성에도 불구하고, 임상실습 교육에 즉각적인 해결이 어려운 많은 문제점이 있음도 이미 알고 있는 사실이다 이에 임상실습 교육의 기초 자료로 활용되고 있는 실습목록표에 대한 간호 학생들의 실습목표의 명확한 이해와 더불어 이론과 실습의 연계성을 강화시키고자 하였다. 성인간호, 모성간호, 이동간호 3개 영역 모두에서 공통적으로 기본임상간호 항목이나 기본간호술과 관련된 항목에서 직접수행의 빈도가 높았고 숙련된 기술적인 처치나 전문성을 필요로 하는 항목에서는 관찰과 수행경험이 부족한 것으로 나타났다. Clinical performance is not only the process of providing the opportunity for applying nursing theory into practice but also it is a well-known fact that clinical performance is an important part in nursing education. lnspite of this importance, it is also true that many problem with no immediate solution exist in clinical performance of nursing students. thus, through measuring and analyzing the degree of clinical experience of the nursing students, on the nursing checklist that is being used as the basic guide in clinical education, we tried to clearly understand the objectives of clinical performance and to emphasize the connection between theory and clinical performance. The results of the study showed that the frequency of directly performing tasks was high in all areas of adult health nursing, maternity nursing, and child nursing for the items of basics clinical nursing and items related with basic nursing techniques, and that the experiences of observation and execution were lacking in the items needing skilled treatment or speciality.

해조류를 이용한 항생재 대체 사료 첨가제 개발

박진용,김진욱,이희현,이송애,김진욱,이재화 신라대학교 자연과학연구소 2005 自然科學論文集 Vol.14 No.-

우리나라의 항생제 남용 문제는 세계적으로 심각 하다고 한다. 이러한 문제를 해결하기 위해서 정부는 다양한 정책을 도입하고 있으며 특히 축산물의 항생제 남용 문제를 해결하기위해 사료 관리법을 강화하여 항생제 사용을 억제하고 있어 항생제를 대체 할 물질이 필요하다. 이런 문제를 해결하기 위해서 우리나라의 다양한 해조류를 이용한 항생제 개발을 통해서 항생제 남용 문제를 해결하고 소비자들에게 믿을 수 있는 축산물을 제공 할 것으로 보인다. Feed official law was strengthened recently. The antibiotic use is suppressed with feed official law reinforcement. The antibiotic transfer material is necessary. To use the seaweeds and transfer the antibiotic. It is a possibility of a high income. This is new bio industry. This the goods production which is competitive power is possible. It is a possibility of eating the livestock where also the consumers are good.

The level of urinary aflatoxin M1 in Korean adults

Yong-Dae Kim1, Hyojin Kwon, Sun-In Moon, Sang-Yong Eom, Jung-Duk Park, Byung-Sun Choi, Seok-Joon Sohn, Young-Seoub Hong, Ho Kim, Ho-Jang Kwon, Ji-Ae Lim, Hae-Jung Yoon, Gwang-Jin Kim, Heon Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Competitive ELISA methods were used to measure the level of aflatoxin M1 (AFM1) from urine in 1008 Korean adults. Subjects were selected by random sampling in all areas of Korea, except Cheju-do. The recovery rate of AFM1 using this method was 105% (73-124%). The geometric mean of urinary AFM1 in all subjects was 3.43 pg/mL (3.67 ng/g creatinine). The level of AFM1 in males was statistically higher, compared with female subjects. However, the levels of AFM1 did not differ according to age. Subjects in Chungbuk-do showed the highest urinary AFM1 concentration, whereas subjects in Kyeongnam-do showed the lowest concentration. Assuming an excretion rate of 5%, this AFM1 excretion corresponds to approximately 0.1 microgram/day in Korean adults.

국내산 과일 및 채소 추출물을 이용한 체중 조절 관련 기능성 식품소재 탐색

김인혜;김종배;조강진;황유진;황경아;고정숙;엄애선 한양대학교 2011 韓國 生活 科學 硏究 Vol.31 No.1

Obesity can be defined as a disorder of lipid metabolism and the control of this process is regulated by enzymes involved in lipid metabolic pathway. Obesity is also characterized increases in the number and size of adipocyte differentiated from fibroblastic pre-adipocytes in adipose tissue. The aim of this study was to screen ethanol extracts of 21 fruits and vegetables cultivated in Republic of Korea for identifying natural sources of lipase inhibitor and to investigate the effects of several extracts resulting in suppression of triglyceride (TG) accumulation in 3T3-L1 cells. Among the tested extracts, Diospyros kaki L. showed the highest contents of total sugar and the content of total phenolic compounds was detected 17 species while there was not detected in Ixeris dentate root and Allium fistulosum C. etc. We were screened 26 extracts of fruits and vegetables for pancreatic lipase inhibitory activity compared with orlistat. The strongest lipase inhibitory activities were shown by extract of Allium sativum L. stem, Allium sativum L. bulb, Apteryx australis, Diospyros kaki L. Malus Pumila, Perilla frutescens and Prunus mume regardless of concentrations treated. Eight extracts including Brassica juncea var. integrilolia had no effect on lipase inhibition. During the differentiation of 3T3-L1 preadipocyte to mature adipocyte, the cells were treated with carrier or 7 extracts(1, 5 and 10 ㎍/ml), which was shown to highest lipase inhibitory effects. We were quantified lipid contents secreted mature adipocytes and stained with Oil Red O. All cells treated with the Apteryx australis and Prunus mume significantly decreased TG contents than the control. These findings suggested that the screened fruit and vegetable could be a promising source as the effective and safe lipase inhibitors for isolating bioactive ingredients and developing functional foods.

매일신보광고를 통해 본 일제시대 한국복식의 변천

金鎭玖,金愛蓮 服飾文化學會 1999 服飾文化硏究 Vol.7 No.2

The purpose of this study is to classify frequency of advertisement and types of advertisement by items and to analyze a primary factors were reflected in the costumes by a policy of rule under the Japanese rule. Data was MaIl Shin Bo's advertisement connected with costumes from 1910 to 1945 years. The results are as follows: 1. The order of the advertisement's frequency was footwear, cosmetics, soap, headgear, western style clothes, precious metals and so on. 2. The type of the advertisement was a format that transmits informs in all items. Cosmetics, hairdye and shampoo applied positive appeals. 3. In the military government, the advertisement's frequency connected with costumes was 37.5% and a shoe store was the first order. A shoes and headgear were high level, because of these were essential imports and were allowded as a proper articles for a western style clothes by a civilized policy. In the political periods of civilization, the advertisement's frequency connected with costumes was 54%. This result indicates industrial development of this period. Soap was the first order during 1924~1933 and cosmetics was the first order during 1934~1940. High level of the advertisement's frequency in these imports were reflected by a cultural policy as a link of an appeasement measure. In the political periods of a racial liquidation, the advertisement's frequency connected with costumes was 8.5% and the advertisement's order by items was cosmetics, a shoe store. The reason was that reflected the phases of the times that was serious by a shortage of goods and an reinforcement of wartime's attitudes throughout war.

브랜드‘더마앤모어’를 위한 스페셜 프로모션 부스 디자인 연구 - ‘프레쉬 캐주얼 스타일링’을 활용하여 -

김수안,박수빈,송혜진,이종화,이진민,장미정,이성애 숙명여자대학교 디자인연구소 2018 숙명디자인학 연구 Vol.25 No.-

본 연구는 (주)애경과 S여대와 산학연계하여 진행한 브랜딩 스타일링 마케팅 기획 프로세스를 체계적으로 정리하는데 목적이 있다. 이에 본 연구는 2017년 9월~12월까지 환경디자인학과 ‘브랜딩·스타일링·마케팅’ 교과목에서 진행한 내용을 대상으로 하며 국내 샴푸브랜드 ‘더마앤모어’를 분석하여 디자인 개념을 결합하고 부스 디자인을 제시하는 과정들을 밝힌다. 이를 통해 본 연구는 향후 생활용품 브랜드의 디자인 개발에 있어서 창의적인 부스디자인의 방향성을 제시한다. 본 연구의 방법은 공간 브랜딩에 대한 이론적 고찰을 통해 브랜딩 스타일링 마케팅 프로세스의 스타일 분석, 디자인 개발, 공간 디스플레이 연출로 구성하여 그 과정을 밝힌다. 끝으로 본 연구는 대학과 산업체간의 연계를 통한 교과목 개발에 활용되는데 의의가 있다.

오감(五感)적 요소를 접목한 브랜드 스페이스 연출 계획 -마임 화장품 프레쉬가든을 중심으로-

김소현,임하영,장효실,최혜원,이진민,장미정,이성애 숙명여자대학교 산업디자인연구소 2017 숙명디자인학 연구 Vol.24 No.-

인간이 갖고 있는 감각 기관은 시각, 청각, 후각, 촉각, 미각의 총 다섯 가지로, 이를 모두 활용 할 때 이상적 공간 상호작용이 가능하다. 본 연구는 화장품의 공간 연출 계획 시 오감적 요소를 접목하여 브랜드 이미지를 구축하는 일련의 프로세스를 밝히는데 목적이 있다. 이에 본 연구는 2017년 3월~6월 15주간 숙명여대 환경디자인학과 <융합연출디자인> 수업에서 진행된 수업의 결과물로서 작업물을 정리하였다. 그 결과 본 연구는 제품이나 서비스를 직접 경험할 수 있도록 다양한 엔터테인먼트적 요소를 결합하여 기능적 만족감과 더불어 체험적 만족감 제공하는 오감적 디스플레이를 제안하였다. 본 연구는 오감을 활 용한 뷰티 쇼룸으로 감각을 공간에 어떠한 방식으로 적용할 것인지에 대한 정립을 했다는 데에 의의가 있다. 아울러 다양한 매체와 공간의 개념이 일반화 되어 가는 오늘날 기업 환경 속에서 오감적 체험을 기반으로 한 공간연출은 가상의 공간속에서도 유연하게 적용 가능한 디스플레이 요소로 확인되었다. 또한 본 연구는 향후 이와 관련된 쇼룸 공간 개발에 있어서 기초 자료로 활용될 것으로 기대된다.