액체크로마토그래피-질량분석기를 이용한 쌀 중 스테리그마토시스틴과 아플라톡신 시험법 개발

이봄내 ( Bom Nae Lee ),권진욱 ( Jin-wook Kwon ),정은아 ( Eun A Chong ),천소영 ( So Young Chun ),강길진 ( Gil Jin Kang ) 한국환경농학회 2019 한국환경농학회 학술대회집 Vol.2019 No.-

Sterigmatocystin is a mycotoxin produced by fungi of the genus Aspergillus (mainly A. nidulans and A versicolor). Sterigmatocystin shares its biosynthetic pathway with aflatoixns. The aim of this study is to optimize the liquid chromatography-tandem mass spectrometry method for determination of sterigmatocystin and aflatoxins (B₁,B₂,G₁,G₂)inrice, simultaneously. Targetsampleswereextractedwith0.1 % formic acid in 50 % acetonitrile then purified with Isolute cartridge (solid phase extraction). Separations by HPLC were mainly conducted with a Cadenza C18 analytical column using 0.1% formic acid and 5 mM ammonium formate under gradient condition with ultrapure water and methanol. The mycotoxins were detected by electrospray ionization in positive or negative ion with multiple reaction monitoring (MRM) and mass spectrometric conditions were optimized in order to increase selectivity and sensitivity. The developed method was validated in terms of linearity, trueness, precision, limits of detection and quantification. Matrix-matched calibration was used for quantification, with regression coefficients (R2) ≥ 0.98.

대장암으로 오인된 장결핵 3례

문봄내,박찬호,한상훤,김철홍,김경욱,최선희,윤중근,설상영 인제대학교 백병원 2003 仁濟醫學 Vol.24 No.1

A lesion which is visualized on colonoscopic examination is rather confusing in that one will find a ulcerative tumor mass occupying the lumen of the bowel which is most suggestive of carcinoma. Especially, tuberculosis of the distal colon occurring in the absence of pulmonary involvement is often mistaken for neoplasm or Crohn's disease. We experienced three cases of colon tuberculosis mimicking a ulcerative tumor mass occupying the lumen of the colon.

상행 결장의 궤양성 종괴를 형성한 복막 결핵 1예

박찬호,문봄내,한상훤,김철홍,김경욱,최선희,윤중근,설상영,정수진 인제대학교 백병원 2003 仁濟醫學 Vol.24 No.1

Tuberculous peritonitis is difficult to diagnose especially in cases without pulmonary tuberculosis, because it is mainly transmitted via hematogenous spreading of tuberculous bacilli from lung. Sometimes this disease is infected directedly by intestinal tuberculosis, female tubuloovarian tuberculosis. We have experienced a case of tuberculous peritonitis presenting as tumorous ulcerative mass penetrating into ascending colon without Intestinal, female genital tuberculosis, which was diagnosed by explolaparotomy and pathologic confirmation from biopsy specimen.

Methamphetamine 중독증상을 보인 환자에서 발생한 횡문근 융해증 및 급성 신부전 1예

권태경,문봄내,최동진,윤기현,김태균,김경욱,김철홍,김민호,이성주,김영훈 인제대학교 백병원 2001 仁濟醫學 Vol.22 No.2

Drugs are important causes of rhabdomyolysis. In Korea, however, there is no report of rhabdomyolysis developed by methamphetamine intoxication. We experienced a case of rhabdomyolysis developed in a methamphetamine poisoning patient. The patient was a 25-year-old man presenting with altered mental status. Results of further investigations showed evidence of acute renal failure and rhabdomyolysis. He was treated with general supportive care including hydration, urine alkalization and hemodialysis, but this patient died eventually. This case shows the importance of the suspiction of illicit drugs including methamphetamine as a cause of rhabdomyolysis with an unclear etiology.

알콜성 간염을 동반한 만성 음주자에 병발된 횡문근융해증 및 급성신부전증 3예

김희승,문봄내,김태균,윤기현,최동진,한상훤,박찬호,김민호,이성주,김영훈 인제대학교 백병원 2001 仁濟醫學 Vol.22 No.2

Acute alcoholic myopathy may present as a fulminant syndrome of muscular pain, swelling and tenderness in extremities associated with rhabdomyolysis and acute renal failure. Acute renal failure caused by rhabdomyolysis and myoglobinuria is one of the serious complications of alcohol and may be lethal occasionally. We report 3 cases of acute renal failure caused by rhabdomyolysis and myoglobinuria associated with alcoholic intoxication, treated in Pusan Medical Center between August 1999 and July 2000.

LC-MS/MS를 이용한 식품 중 맥각 알칼로이드 시험법 개발

천소영,정은아,이봄내,권진욱,박혜영,김신희,강길진 한국식품위생안전성학회 2019 한국식품위생안전성학회지 Vol.34 No.2

Ergot alkaloids are mycotoxin produced by fungi of the Claviceps genus, mainly by Claviceps pur- purea in EU. Recently obtained informations indicates necessity for control the ergot in imported grains. Recent occurrence data of ergot alkaloids from EU countries indicate the necessities of management and control these toxins from the imported grains like rye, wheat, oat etc. The aim of this study is to optimize the liquid chromatography-tandem mass spectrometry method for determination of ergot alkaloids (ergometrine, ergosine, ergotamine, ergocornine, ergocryptine, ergocristine and their epimers (-inines) from grain and grain-based food. The test method was optimized by extracting the sample with ace- tonitrile containing 2 mM ammonium carbonate, purification with Mycosep cartridge, and instrumental analysis by LC-MS/ MS using Syncronis C18 column. The standard calibration curves showed linearity with correlation coefficents; R2 >0.99. Mean recoveries ranged from 72.0 to 111.3% at three different fortified levels (20, 50, and 100 µg/kg). The correlation coef- ficient expressed as precision was within the range of 1.9-12.9%. The limit or quantifications (LOQ) ranged from 0.012 to 0.058 µg/kg. The developed analytical method met the criteria of AOAC Int. and CAC validation parameters like accuracy and sensitivity. As a result, it was confirmed that the test method developed in this study is suitable for the simultaneous analysis of six species of ergot alkaloid from grains and grain products.

장출혈을 동반한 Henoch-Scho¨lein Purpura 1예

김태균,윤기현,최동진,문봄내,한상훤,박찬호,최선희,윤중근,설상영 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

Henoch-Schonlein purpura(HSP) is a systemic, generalized vasculitis of unknown etiology, involving skin, joint. GI tract, and kidney. Although it can occur from age 6 months to adulthood, 50% of cases are children under 5 years of age and 75% are under 10 years. GI symptoms appear in more than half of affected children. However, in the adult age range, GI symptoms occur in approximately half of the cases of HSP and melena was observed in about 30% of patients. We report a case of a patient with HSP who presented with lower GI bleeding.

뇌졸중 환자의 자가간호 수행정도와 가족의 교육적 요구에 관한 연구 : Care and Their Family Members' Needs for Education

이은화,김진숙,박은주,박혜진,최봄내 이화여자대학교 간호과학연구소 2002 Health & Nursing Vol.14 No.2

About 25% of the stroke patients die within a month after attack, and even the patients who survive will have motor, sensory, cognitive dysfunction or speech defect, and therefore, they should continue to be taken care of. Under such circumstances, this study was aimed at reviewing how the stroke patients requiring a long-term nursing were caring themselves and what were their family members’ needs for education and thereby, explore the ways for the nurse to intervene in their care. To this end, a questionnaire survey was conducted by sampling the stroke patients hospitalized at‘E’University hospital in Seoul for 3 or 4 days their 75 family members. The data were collected for 57 days form July 14 to September 8, 2001. The stroke patients’self-care measured b using and ADL scale developed b Kang Hyeon-sook(1984), while their patients’needs were measured by using a scale developed by Han Jeong-seok and Seo Mi-hye(1992) and complemented by Kim Mi-hee (1997) and adapted by the researcher for this study. The collected data were analyzed using SPSS/PC+ 10.0 program for real numbers, percentages, means, standard deviations, ANOVA and Pearson’s coefficients. The results of this study can by summarized as follows; 1) The stroke patients' self-care scored 2.52(±1.02) out of 4 perfect score. 38.1% of the patients were 'completely dependent’on their family members for treatment, while 64.2% of them felt it difficult to nurse themselves. 2) Patients’family members’needs for education scored 4.11(±.78) out of 5 perfect score. The factor which scored highest was 'information related with treatment’(4.39±.76), followed by ‘nursing requirement due to immovability’(4.05±.91), 'nursing related with ordinary life’(3.99±.85) and‘social, psychological and financial problems’3.92±.04) in their order. 3) When analyzed in terms of patients’demographic variables and characteristics of the disease, patients’self-care was significantly higher when there were no grown-up children[F(1, 73)=4.115, p=0.46], but there was found some significant difference depending on the number of troubles[F(5,73)=2.562, p=0.35]. But the patients'self-care was significantly lower when they suffered from a paralysis[F(1, 73)=4.072, p=.047] or when they suffered form a cerebral troubles[F(1, 73)=19.410, p=.000]. When analyzed in terms of patients’demographic variables and disease types, their family members’needs for education were highest when the patients suffered from both Middle Cerebral artery(MCA) and Brain Stem(B.S.) symptom, followed by when they suffered form MCA and when they suffered from B.S. trouble in their order.[F(2, 73)4.092, p=.021]. On the other hand, their needs for education were also high when the patients suffered from a paralysis[F(1, 73)=5.983, p=.017] and a speech defect.[F(1. 73)=4.572. p=.036]. 4) When analyzed in terms of family members’demographic variables, it was found that their educational needs was positively correlated with their educational background[F(3, 73)=3.731, p=.015] and religion, in particular Buddhism.[(4, 73=4.208, p=.004). 5) The stroke patients’self-care was negatively but not significantly correlated with their family members’needs for education.(r=-.113, p=.334). As discussed above, it was confirmed through this study that stroke patients and their family members were much concerned about the prognosis of the disease and self-care, while the family members' needs for education were very high beginning form the time when the patients were hospitalized. Therefore, it is necessary to develop an stroke patients' educational program in consideration of their family members' needs for education and priorities, while exploring an effective way to educate their family members about self-care at the initial stage of hospitalization. All in all, it is also deemed necessary to review the current nursing curriculum about nursing intervention and education of family members for stroke patients and thereby, perform and effective nursing intervention for them.

자궁내 피임 장치와 동반된 복부 및 골반 방선균증 1예

최동진,윤기현,김태균,문봄내,박찬호,한상훤,이민재,윤중근,이세용,설상영 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

Actinomycosis is an uncommon chronic, suppurative granulomatous disease, caused by an anaerobic bacterium. Actinomyces israeli, which is a component of human oral and gastrointestinal flora. The cervicofacial region is the commonest site of disease, and the abdominopelvic region is the second commonest site. In most cases the diagnosis is made postoperatively because of its unusual clinical presentation. Although the incidence of actinomycosis has decrease, the abdominal-pelvic form has been increasing over the past several years secondary to increased prolonged use of the intrauterine device. We report here the case of a patient with the most severe rare form of abdominal wall and pelvic involvement, characterized by dense adhesions between large and small bowel and large bowel and uterus, adnexa, and bladder associated with intrauterine contraceptive device.

폐광산 인근 지역에서 생산되는 농산물의 중금속 오염도 평가

안재민,장순영,황향란,박대한,이봄내,김샛별,이광희 한국환경농학회 2020 한국환경농학회지 Vol.39 No.1

BACKGROUND: This study was to carry out risk assessment of contamination of cadmium (Cd), lead (Pb), and inorganic arsenic (I-As) in agricultural products of 25 crops from the abandoned mine areas. The 36 typical investigation sites located in Gyeongbuk provincial area were selected by considering the heavy metal levels, that had been known that the amount of the heavy metals exceeded the contamination level based on the previous survey. METHODS AND RESULTS: Cadmium, lead, and total arsenic (T-As) concentrations were determined using microwave device and ICP-MS. Inorganic arsenic was determined by HPLC-ICP-MS. The limits of quantification for heavy metals were 0.59 μg/kg for Cd, 0.42 μg/kg for Pb, 0.55 μg/kg for T-As, and sum of As (Ⅲ) (1.74 μg/kg) and As (Ⅴ) (2.25 μg/kg) for I-As, respectively. The contents of Cd, Pb, and I-As (only rice) were N.D.-0.958 mg/kg, N.D.-0.227 mg/kg, and 0.082 mg/kg, respectively, in the agricultural products. For risk assessment, dietary exposures of heavy metals through usual intake were 5.20×10-4-7.15×100 μg/day for Cd, 7.00×10-5-7.75×10-1 μg/day for Pb, and 1.17×101 μg/day for I-As, taking 0.01-14.37%, 0.01-2.05%, and 15.16% as risk indices, respectively. CONCLUSION: It requires to consider the critical levels of heavy metals in agricultural products due to unexpectedly high levels in a few places, while concentrations of heavy metals in the samples were relatively low in most areas. .