F-93 : Free Paper Presentation ; The Role of WNT Signaling in Chronic Murine Asthma Model

곽현정,이성자,박동원,문지용,김태형,손장원,윤호주,신동호,박성수,김상헌 대한결핵 및 호흡기학회 2013 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.116 No.-

Background: Airway remodeling is a main pathogenesis of impaired lung function of asthmatic patients and it is presented as subepithlial fibrosis and hypertrophy of smooth muscle. WNT signaling pathway might be suggested as a critical determinant of airway remodeling in asthmatic patients but there were few reports about direct association between asthma and WNT signal. Thus, we made a murine chronic asthma model and aimed to find out relationship between WNT signaling pathway and airway remodeling in pathogenesis of asthma. Methods: BALB/c mice, after intraperitoneal ovalbumin (OVA) sensitization on days 0 and 7, received intranasal OVA periodically days 14-94. Samples of murine lung tissue for histology and quantitative real-time PCR of WNT family, TNF-β, SMA, PDGFR, Tenscin C and SM22α, and β-catenin were obtained. Using SiRNA for β-catenin, we blocked the canonical pathway and review the tissue and samples of chronic murine asthma model. Results: The OVA-treated mice developed an extensive eosinophil and mononuclear cell inflammatory response, subendothelial fibrosis and hypertrophy of airway smooth muscle in the chronic murine asthma group. The real-time PCR and immunohistochemistry revealed that β-catenin, WNT5a, WNT7a, TNF-β, SMA, PDGFR, Tenscin C and SM22α were up-regulated in chronic asthma group and lower uptake level of them were shown in SiRNA-treated groups. Conclusion: These findings suggest that WNT/β-catenin signaling may be a contributor of airway remodeling in chronic asthma via both pathways of fibrosis and hypertrophy of smooth muscle.Background: Airway remodeling is a main pathogenesis of impaired lung function of asthmatic patients and it is presented as subepithlial fibrosis and hypertrophy of smooth muscle. WNT signaling pathway might be suggested as a critical determinant of airway remodeling in asthmatic patients but there were few reports about direct association between asthma and WNT signal. Thus, we made a murine chronic asthma model and aimed to find out relationship between WNT signaling pathway and airway remodeling in pathogenesis of asthma. Methods: BALB/c mice, after intraperitoneal ovalbumin (OVA) sensitization on days 0 and 7, received intranasal OVA periodically days 14-94. Samples of murine lung tissue for histology and quantitative real-time PCR of WNT family, TNF-β, SMA, PDGFR, Tenscin C and SM22α, and β-catenin were obtained. Using SiRNA for β-catenin, we blocked the canonical pathway and review the tissue and samples of chronic murine asthma model. Results: The OVA-treated mice developed an extensive eosinophil and mononuclear cell inflammatory response, subendothelial fibrosis and hypertrophy of airway smooth muscle in the chronic murine asthma group. The real-time PCR and immunohistochemistry revealed that β-catenin, WNT5a, WNT7a, TNF-β, SMA, PDGFR, Tenscin C and SM22α were up-regulated in chronic asthma group and lower uptake level of them were shown in SiRNA-treated groups. Conclusion: These findings suggest that WNT/β-catenin signaling may be a contributor of airway remodeling in chronic asthma via both pathways of fibrosis and hypertrophy of smooth muscle.

W^(sh)/W^(sh) 마우스 고환에서 Protein Kinase C(PKC)와 c-kit의 발현 양상

곽현정,최병민,서기석,임정식,정헌택 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3

Protein kinase C (PKC) is a multigene family of at least nine serine/threonine kinases that are central to many signal transduction pathways. And the expression of PKC gene is strictly con-trolled by developmental stage. Mutation of the W (c kit) gene, which encodes a transmembrane tyrosine kinase receptor, affect the development and differentiation stem cells. Most homozygous W mutant mice are sterile, due to a lack of germ cells arising during embryonic development, but one of notable exception is W */Wh mice, which are fully fertile in both sexes. In order to elucidate the biological functions of PKC S and c-kit in the testes, we have examined the expression of PKC b mRNA and c-kit mRNA in While mouse testes by means of Northern blotting. North-em blot results was demonstrated that only PKC 8, 4, 8 mRNA can be detected in normal mouse testes. PKC, 8 mRNA was weakly expressed in 7 and 8-week-old mice and highly ex-pressed by 12 weeks. The PKC 8 mRNA and the c-kit mRNA was detectable in the testes of Whl Wh mouse. These results indicated that the function of the PKC a gene is necessary for the development and the W (c-kit) gene is important for the transmission of signal in testes.

The Extended Rapid Response System: 1-Year Experience in a University Hospital

곽현정,윤인아,김상헌,손장원,신동호,윤호주,김근호,Tchun Young Lee,박성수,임영효 대한의학회 2014 Journal of Korean medical science Vol.29 No.3

The rapid response system (RRS) is an innovative system designed for in-hospital, at-riskpatients but underutilization of the RRS generally results in unexpected cardiopulmonaryarrests. We implemented an extended RRS (E-RRS) that was triggered by actively screeningat-risk patients prior to calls from primary medical attendants. These patients wereidentified from laboratory data, emergency consults, and step-down units. A fourmemberrapid response team was assembled that included an ICU staff, and the teamvisited the patients more than twice per day for evaluation, triage, and treatment of thepatients with evidence of acute physiological decline. The goal was to provide thistreatment before the team received a call from the patient’s primary physician. We soughtto describe the effectiveness of the E-RRS at preventing sudden and unexpected arrestsand in-hospital mortality. Over the 1-yr intervention period, 2,722 patients were screenedby the E-RRS program from 28,661 admissions. There were a total of 1,996 E-RRSactivations of simple consultations for invasive procedures. After E-RRS implementation,the mean hospital code rate decreased by 31.1% and the mean in-hospital mortality ratewas reduced by 15.3%. In conclusion, the implementation of E-RRS is associated with areduction in the in-hospital code and mortality rates.

Williams 증후군 환자의 마취경험

곽현정,김지영,이경천,김종찬,이강훈,곽영란 대한마취과학회 2005 Korean Journal of Anesthesiology Vol.48 No.3

부와의 사별로 인한 PTSD의 중년 여성의 사례

곽현정 한국모래놀이치료학회 2014 모래놀이상담연구 Vol.10 No.1

본 사례는 부와의 사별로 인한 PTSD의 중년 여성 사례로 총 46회기의 모래놀이치료를 실 시하였다. 본 사례연구의 목적은 내담자가 모래놀이치료를 통해 PTSD증후군과 부정적인 부성 콤플렉스와 모성콤플렉스를 변환시켜나가는지를 보고자 하는 것이다. 내담자는 부와의 사별 후 늘 우울한 감정과 심한 불안감이 있었다. 부성적인 결핍을 책과 경쟁에서 채우려 하였다. 또한 내담자의 자아상은 10세의 어린 소녀로 남편과의 관계, 육아의 어려움을 호소하였다. 모 래놀이치료가 진행되면서 내담자의 어린 자아가 성숙되는 과정을 볼 수 있었으며 어린 자아 가 성숙해 질수록 내담자의 얼굴이 밝아지고, 남편, 딸과의 관계도 자연스러워졌다. 또한 부와 의 사별로 인한 PTSD증후군과 부정적인 부성콤플렉스, 모성콤플렉스가 변화되어가는 것을 볼 수 있었다. 본 사례 연구를 통해 모래놀이치료가 부와의 사별로 PTSD증후군과 부정적인 부성 콤플렉스와 모성콤플렉스의 문제를 가진 여성에게 효과적인 치료방법임을 재확인 할 수 있었 다.

식품제조공장 내 공중부유미생물 오염도와 오염진균동정

곽현정,이헌준,이상호,나혜진 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.4

To evaluate the indoor air quality of food manufacturing plants, the presence of viable bacteria and fungi was assessed in the indoor air of the facilities at which 9 food items were manufactured. Air samples were collected from the general zone, low clean zone and clean zone of each factory with an air sampler, in combination with plate counts agar using for bacteria, and dichloran-glycerol agar for fungi. The samples were incubated at 25℃for 4 to 7 days. After culture, the colony forming units (CFU) on each plate were counted and corrected with a positive hole conversion table. The average concentration of bacteria was 2.2 × 10³ CFU/㎥ in the general zone, 1.2 ×10³ CFU/㎥ in the low clean zone and 7.3 × 10² CFU/㎥ in the clean zone. The average concentration of fungal microbes was 2.5 × 10³ CFU/㎥ in the general zone, 2.6 × 10³ CFU/㎥ in the low clean zone, and 2.0 × 10² CFU/㎥in the clean zone. No meaningful differences were detected between the general zone and the low clean zone, but the clean zone had significantly lower concentrations than the other zones. Additionally, the identification of the fungi was performed according to morphological method using a giant culture and slide culture. The fungi were identified as belonging to 18 genera, and the genera Cladosporium(33%), Penicillium(29%) and Aspergillus(26%), predominated. Aspergillus isolates were identified to species level, and A. ochraceus, a mycotoxigenic species, was identified. As part of the effort to control the quality of the indoor air of food manufacturing plants, our results show that continued studies are clearly warranted.

내시경하 부비동 수술을 받는 Churg-Strauss 증후군 환자의 전정맥마취 경험 -증례 보고-

곽현정,이동철,최성호,이경천 대한마취통증의학회 2010 Anesthesia and pain medicine Vol.5 No.1

Churg-Strauss syndrome is also known as allergic granulomatosis,and it is a rare systemic vasculitis that may affect multiple organ systems, and particularly the lungs. A 42-year-old woman with Churg-Strauss syndrome underwent endoscopic sinus surgery under total intravenous anesthesia with propofol and remifentanil. Anesthesia was performed uneventfully, but there were several issues of concern that evolved regarding the perioperative management of a patient with this syndrome.

Atrial flutter associated with high pressure pneumoperitoneum during laparoscopic gastrectomy -A case report-

곽현정,이경천,김홍순,김승환,이동철 대한마취통증의학회 2010 Anesthesia and pain medicine Vol.5 No.1

Pneumoperitoneum associated with carbon dioxide (CO2) insufflation may induce alterations in electrocardiographic variables, which may produce severe atrial and ventricular arrhythmias. We now present a case in which atrial flutter developed after CO2 insufflation in a patient undergoing laparoscopic subtotal gastrectomy. Although the ECG change was reversed after desufflation, one should keep in mind the increased propensity to atrial and ventricular arrhythmias during laparoscopy with high intra-abdominal pressure.

COPD Assessment Test (CAT) and Declines of Lung Function in COPD Patients

곽현정,김사일,김상헌,김태형,손장원,윤호주,신동호,박성수 대한결핵 및 호흡기학회 2011 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.112 No.-

유양돌기절제술 중 예기치 않게 발생한 폐혈전색전증

곽현정,박희연,김홍순,이경천,최성호 대한중환자의학회 2009 Acute and Critical Care Vol.24 No.3

We report on a 45-year-old patient who sustained an intra-operative pulmonary thromboembolism during elective mastoidectomy under general anesthesia. At the end of surgery, the patient developed hemodynamic compromise and exhibited T wave inversion on electrocardiogram. Echocardiography showed an echogenic mass in the right pulmonary artery and pulmonary hypertension. Pulmonary thromboembolism is rare in the field of otolaryngology and head and neck surgery; however, it may develop, resulting in a fatal outcome. It is thus important to establish the diagnosis early and prevent such serious complications.