한국인 인슐린 비의존형 당뇨병 및 이차성 당뇨병 환자에서 글루코키나제 유전자 변이

남재현(Jae Hyun Nam),이현철(Hyun Chul Lee),김연의(Youn Euy Kim),권석호(Suk Ho Kwon),윤용석(Yong Suk Yoon),박석원(Suk Won Park),원영준(Young Jun Won),차봉수(Bong Su Cha),송영득(Young Duk Song),이은직(Eun Jig Lee),임승길(Sung Kil Lim) 대한내과학회 1998 대한내과학회지 Vol.54 No.6

N/A Objectives: Mutations in the glucokinase (GCK) gene are considered a possible cause of maturity-onset diabetes of the young. The purpose of this study was to evaluate the contribution of this gene to the development of non insulin dependent diabetes mellitus (NIDDM), gestational diabetes mellitus (GDM) and post-renal transplantation diabetes mellitus (PTDM). Method: Identification of GCK mutation was attempted on 39 NIDDM patients, 2 GDM patients and 58 selected renal allograft recipients with PTDM and 45 normal controls. The exons in the GCK gene were examined by polymerase chain reaction (PCR), followed by analysis of single-stranded DNA conformational polymorphism (SSCP). The abnormal bands were also confirmed by DNA sequencing analysis. The exons of affected family members were also investigated for mutations of the GCK gene. Results: Two of the 58 PTDM patients (3.4%) were found to have CKK mutations. One had the mutation on exon 5 and the other on intron 7. One control subject had the mutation on intmn 9. The mutation of exon 5 was identified as a substitution of CCT (proline) for CTT (leucine) at codon 164, which has not ever reported before. The family members of the PFDM patient with mutation of exon 5 were analyzed by PCR followed by SSCP, and two of them revealed the same mutation. The abnormal band on the SSCP analysis of exon 7 was identified as the insertion of base C/T at the 39th nucleotide in intron 7. Two family members of this patients also had same band on SSCP. The one mutation of 45 normal controls was CT located at the 8th nucleotide in intron 9, which was a common polymorphism. Conclusion: We found GCK mutations in subjects with PTDM and we speculate that these mutations may be one of the contributing cause of PTDM.

주사전자현미경을 이용한 무당벌레에 기생하는 무당벌레기생파리(Degeeria separata)의 외부형태적 특징

윤영남,김남성,김기덕,박수진,서미자,채순용 충남대학교 농업과학연구소 2000 농업과학연구 Vol.27 No.1

External morphological characteristics of male and female of Degeeria separata, which was endoparasited in adult of the Asian ladybird, were studied by using the scanning electron microscopy(SEM). The antenna of Degeeria separata was consisted of three segment and arista. Anstae were about 750㎛ length and 30㎛ in diameter. The surface of antennae was covered with many sensilla. They were long or short, slender or thick, and straight or curved. Each three morphological types of sensilla were observed on the second and third segment of antennae. The mouthpart was sponging type. It was consisted with clypeus, labellum, labium, labrum, and maxillary palp. The labellum is 400㎛ width and double folded vertically. The leg consist of tarsus, tarsus claw, pulvilli, and empodium.

주사전자현미경을 이용한 토마토에 발생하는 아메리카잎굴파리(Liriomyza trifolii)의 외부형태적 특징

윤영남,김기덕,박수진,김남성,서미자,채순용 충남대학교 농업과학연구소 2000 농업과학연구 Vol.27 No.1

SEM을 이용하여 아메리카잎굴파리(Liriomyza trifolii)의 암 ·수컷 성충의 외부형태적 특징을 살펴보았다. 더듬이에 있어 감각모의 3가지 형태는 더듬이가는마디(funicle)에서 관찰되었으며, 모상감각기(trlchold sensillum)는 말단 부위가 뽀족한 특징적인 형태를 하고 있고 감각기들 중에서 가장 많이 분포하고 있다. 원추상 감각기(basiconic sensillum)는 길이가 13㎛, 지름이 1.4㎛에서 1.2㎛인 원형곡선형이다. 홈형 감각기(grooved sensillum)는 간상(peg) 끝에서 나온 손 모양을 하고 있으며, 이 모든 감각기들은 후각과 연관되어져 있는것으로 추정된다. 구기는 홉즙형으로 작고 펼쳐진 윗입술(labrum)과 합쳐져서 하나의 연결된 주둥이(rostrum)을 형성하고 있다. 가느다란 한 쌍의 작은턱수염(maxillary palp)은 긴 관 모양으로 각각의 세로로 이어져 있다. 발마디는 5마디로 이루어져 있으며 그 한가운데에는 패드모양의 흡착반(pulvillus)과 가시모양의 발톱사이돌기(empodium)가 있다. 아메리카잎굴파리는 흡착반과 발톱사이돌기에서 미끄러운 표면에 부착할 수 있는 물질을 분비한다. 수컷의 생식기는 매우 복잡하며 삽입구(aedeagus)는 생식기관으로서 가장 일반적인모양이다. 반면에 암컷의 생식기는 상대적으로 간단한데 이 구조는 외부로 돌출된 관모양이었다. 또한 이러한 결과를 이미 발표된 다른 여러 종의파리와 비교하였다. The external morphology of male and female of Liriomyza trifolii (Burgess) was studied using the scanning electron microscopy (SEM). Three types of sensilla were identified on the funicle of antennae. The trichoid sensilla were most conspicious and distributed with a characteristic sharp structure at the apical end. Basiconic sensilla were showed distal curvature about 13㎛ long and 1.2㎛ to 1.4㎛ in diameter. Grooved sensilla were showed a finger-like projections at the peg apex. The function of these sensilla likely to be olfactory. The mouthparts were adapted for sucking food, and formed a elongate rostrum which was incorporated with labrum. The labrum was a small and flap-like shape. The slender and paired maxillary palp seemed to combine with each other lengthwise to long tube. The tarsus typically consisted of 5 segments, which might be variously modified, principally by thickening or flattening. The segment healed a pair of claws. Beneath the claws, there was a pair of pad-like pulvillia and a bristle-like median empodium. L. trifolii secreted on the pulvillus and empodium an adhesive substance that enable them to cling to smooth surfaces. Male terminalia was complicated. The name 'aedeagus' seemed the best available general term for the copulatory organ, otherwise, female terminalia was usually relatively simple. This structure was reduced and formed a telescopic, eversible, and tubular ovipositor.

마스낵 제조를 위한 당절임 공정의 최적화

한주영,김남우,황성희,윤광섭,신승렬 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

대부분의 마는 건조분말제품으로 가공되고 있는데, 마를 다양한 가공품으로 개발하기 위한 방법의 하나로 삼투건조라는 전처리를 행하여 건조에 의한 품질손상을 억제하고 단맛으로 기호성을 높인 건조 마 제품을 얻을 수 있는 당절임 공정의 최적화를 시도하여 최적조건을 얻고자 하였다. 침지시간을 3-7시간, 온도는 20-60℃, 당농도는 30-70%로 하는 중심합성계획법으로 실험을 설계하여 최적화하였다. 이때의 종속변수로는 침지 후 수분함량, 당도, 색도, 그리고 동결건조후의 수분함량과 재수화율로 하여 분석한 결과 동결건조 후 수분함량에 대해서는 유의성이 없었다. 세 가지의 공정변수 중 온도의 영향이 가장 적어 온도를 중심으로 고정한 후 침지시간과 당농도의 최적조건을 찾은 결과, 수분함량을 66-70%, 당도를 25-30%, L 75이상, a-2.1-2.4, b를 5이상 그리고 재수화율을 200-250을 제한 조건으로 하는 조건으로 하는 영역은 5.2-5.9시간, 56-61%로 나타났다. This study was conducted to the optimize sugaring process of yam for development of new snack product and enhancement acceptability. Three variables by five level central composite design and response surface methodology were used to determine optimum conditions for sugaring time, temperature and concentration. Optimization of the process was conducted using the combination of the moisture content, solid content, color and rehydration ratio. The regression polynomial model was suitable (P>0.05) model by Lack-of-Fit analysis with highly significant. To optimize the process, based on surface response and contour plots, superimposing the individual contour plots for the response variables. The optimum conditions for this process were 5.5 hours and 58% at 40℃ under the optimum of restricted variables as moisture content was 66 to 70, solid content was 25 to 30%, L value was above 75, a value was -2.1 to -2.4, b value was above 5 and rehydration ratio was 200 to 250.

곤충

장영덕,윤영남,장철,이만영 忠南大學校 環境問題硏究所 1992 環境硏究 Vol.10 No.-

1992년 9월 4일부터 5일 까지에 전라북도 진안군에서 조사된 곤충류는 총 9목 58과 176종이었다. 조사된 종류중 노린재목이 41종으로 가장 많았으며 딱정벌레류는 빈약한 편이었다. 또한 남부지방의 타지역에 비해 특기할 만한 종이나 현상은 없는 편이었다. 종다양도는 site A1에서 34.167로, 우점도는 site Y3에서 0.779로, 종풍부도는 site S1에서 4.327로 가장 높게 나타났으며 지역간 유사도는 인정되지 않았다.

바륨 페라이트를 함유한 알지네이트 자성 복합 입자의 제조

이덕연,오영일,김동현,김광만,김경남,이용근 大韓齒科器材學會 2004 대한치과재료학회지 Vol.31 No.1

Magnetic polymer composite materials with high coercivity have potential applications in medical diagnostic technologies, magnetic drug delivery, and hyperthermic cancer treatment. The purpose of this study was to prepare the magnetic composite particles by encapsulating barium ferrite powders with alginate and to investigate their physical and biological properties. 0.4 g of sodium alginate was dissolved in 20 ml of distilled water and different amount (0.4~4 g) of barium ferrite powder was dispersed in alginate solution using an ultrasonication method. The resulting barium ferrite-alginate slurry was added dropwise to a CaCl2 solution to form magnetic Ca-alginate composite beads. They were separated by filtration and washed several times with water and ethanol. The final product was then completely dried at 40℃ under vacuum to obtain brownish black powders (0.1~1 mm in size) with a high magnetic response when submitted to a small magnet. Average size of magnetic composite particles was dependent on the amount of barium ferrite and the viscosity of slurry . The morphological and dimensional analyses of magnetic composite particles were performed by optical microscopy and scanning electron microscopy. Magnetic polymer composite particle was found to maintain the high coercivity. The saturation magnetization value for magnetic Ca-alginate composites increased with the increase of the barium ferrite/alginate ratio. The amount of self-heating induced by hysteresis under an alternating magnetic field was measured as a function of barium ferrite/alginate ratio in distilled water. According to the results, the encapsulated magnetic composite is expected to be useful for hyperthermia and chemotherapy remarkably.

산딸나무 열매로부터 저밀도 지질 단백질(LDL) 산화 역제물질의 분리

이대영,송명종,김민정,정낙훈,정태숙,이윤형,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

산딸나무 열매를 100% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복하여 1종의 lignan 화합물을 분리, 정제하였다. 화합물의 화학구조는 NMR, Ms 및 IR 등의 스펙트럼 데이터를 해석하여, (+)-pinoresinol (1) 로 동정하였다. 이 화합물은 산딸나무에서 처음 분리되었다. LDL-항산화활성 억제활성이 20㎍/mL 처리농도에서 85.1±1.3%로 나타났다. (+)-Pinoresinol was isolated from the EtOAc fraction of methanol extracts of the fruits of Cornus kousa Burg. The chemical structures of (+)-pinoresinol was determined by spectroscopy method, as well as 1D- and 2D-NMR (^(1)H-^(1)H COSY, HSQC and HMBC) spectroscopy. This compound was the first to be isolated from C. kousa Burg. Also, (+)-pinoresinol inhibited LDL-oxidation with the inhibitory activity of 85.1±1.3% at a concentration of 20 ㎍/mL.

황금(Scutellaria baicalensis) flavonoid의 대량 분리

곽호영,김동현,이윤형,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

황금의 뿌리를 물 또는 80% MeOH 로 추출하고 각각 농축하였다. MeOH 추출물은 물, EtOAc 및 n-BuOH로 분배 추출하였다. 얻어진 분획물에 대하여 silica gel column chromatography 하거나, 침전 및 여과과정을 반복하여 4종의 flavonoids 즉, baicalin, baicalein, wogonin 및 wogonoside를 높은 수율로 분리하였다. 분리한 화합물의 화학구조는 NMR을 포함한 물리화학적 data를 해석하여 동정하였다. The root of Scutellaria baicalensis was extracted with water or 80% aqueous MeOH and concentrated, respectively. The MeOH extract was partitioned between H_(2)O with organic solvents such as EtOAc and n-BuOH. The silica gel column chromatography or the precipitation method led to the isolation of four pure flavonoids, baicalin, baicalein, wogonin and wogonosides, with very high yield. The identification of the obtained flavonoids was carried out from the result of physico-chemical data including NMR.

韓國産 진디벌 未記錄 14種에 대하여

張英德,尹泳南 忠南大學校 環境問題硏究所 1983 環境硏究 Vol.1 No.2

Specimens of Aphidiidae were collected in the area of Daejeon and Mt. Gyeryong in Korea, during the period from April to September, 1983. Described 14 species of Aphidius areolatus Ashmead, Aphidius cingulatus Ruthe, Aphidius coloratus Baker, Aphidius pisivorus Smith, Binodoxys nearctaphidis Mackauer, Bioxys japonicus Stary & Schlinger, Boreogalba gladifer Mackauer, Ephedrus californicus Baker, Lipolexis scutellaris Mackauer, Lysiphlebus salicaphis(Fitch), Monoctonus similis Stary & Schlinger, Trioxys (Binodoxys) indicus Subba Rao & Sharma, Trioxys (Trioxys) asiaticus Telenga and Trioxys hokkaidensis Takada were for the first time in Korea.

함치성 낭의 임상적 및 방사선적 특성

박성연,남동우,김영진,김현정,남순현 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

이 연구의 목적은 함치성 낭의 임상적 및 방사선적 특성을 세 그룹으로 나누어 살펴보고자 하였다. Ⅰ, Ⅱ, Ⅲ군으로 나누었으며 첫 번째 Ⅰ군은 함치성 낭에 이환된 부위가 유치 하방의 계승 영구치인 군이며, Ⅱ군는 이환부위가 영구 대구치 부위인 군이며, Ⅲ군은 이환부위가 상악 전치부의 과잉치인 경우로 나누었다. 저자는 Ⅰ군의 49증례와 Ⅱ군의 36증례, Ⅲ군의 15증례들에 대하여 이들의 병록자와 파노라마 방사선 사진에 기초하여 임상적 및 방사선적 특성은 비교, 관찰하였다. 연구 결과는 다음과 같았다. 1. 유치 하방의 영구 계승치를 포함하는 Ⅰ군의 49증례로 가장 많았고, Ⅱ군과 Ⅲ군이 각각 36증례, 15증례였다. 2. 함치성 낭은 전체적으로 10대에 가장 호발하였으며, 계승치군인 Ⅰ군에서는 10대 이전과 초반에 87.8%, 대구치 군과 과잉치 군인 Ⅱ군과 Ⅲ군에서는 이보다 늦은 시기에 호발하였다. 3. 남자가 여자보다 2,5배 높은 발생 빈도를 보였다. 4. 환자의 내원 동기는 종창이 50%로 가장 많았고, 정기 검진(32%), 동통(9%) 순이었다. 5. 낭의 유형은 Ⅰ군에서 lateral type이 71.4%로 많았고, Ⅱ군과 Ⅲ군에서는 central type이 각각 94.4%, 100%로 많았다. 6. 병소의 크기는 Ⅰ군에서는 2치관 크기가, Ⅱ군에서는 1치관 크기가, Ⅲ군에서는 4치관 크기 이상이 많았다. 7. 이환된 치아의 대부분은 변위를 보였고, 변위된 치아의 일부에서 치근 발육이 지연되거나 치근 만곡 경향을 보였다. 8. 낭의 성장에 의한 치조골 반응은 Ⅰ근에서는 협측골 팽창(67.3%)이 많았고, Ⅱ군에서는 골팽창이 일어나지 않은 경우(66.7%)가 많았으며, Ⅲ군에서는 구개측 골팽창(60.0%)이 많았다. 9. 낭에 포함되는 치아로는 하악 제 3대구치가 31%로 가장 많았고 하악 제 2소구치(30%), 상악 전치부 과잉치(15%), 상악 견치(8%), 하악 제 1소구치(5%) 순이었다. 10. Ⅰ군에선 추정되는 낭의 발생 원인으로는 치수절단술을 받은 유치가 59.2%로 가장 많았고 이외에 심한 우식 및 치료받지 않은 외상 병력 등 기타 가능한 원인들이 있었다. 11. 함치성 낭의 치료법으로는 Ⅰ군의 경우 61.2%에서 조대술이 시행되었고, Ⅱ군과 Ⅲ군의 경우 61.1%, 80.0%에서 적출술이 시행되었다. The purpose of this study was t o discriminate clinically and radiographically among the three groups of dentigerous cysts studied. First, Group I , involved area of dentigerous cyst was successive permanent tooth area beneath deciduous tooth. Second. Group Ⅱ, involved permanent molar area, and the last. Group Ⅲ, involved maxillary anterior supernumerary tooth area. The author observed and compared the clinico-radiographic features of 49 cases of Group I , 36 cases of Group Ⅱ. and 15 cases of Group Ⅲ of dentigerous cyst and this observation and comparison had been done by based on the charts and panoramic films. The obtained results were as follows: 1. The cases of Group 1 mere 29 cases and, those of Group Ⅱ were 36 and those of Group Ⅲ were 15. 2. The incidence of dentigerous cyst is high in first decade. In Group I, before first decade and early first decade was 87.8%. in Group Ⅱ and Group Ⅲ, was discovered more lately. 3. The frequency of dentigerous cyst is 2.5 times higher in male than in female. 4. The sequence of chief complaint was swelling(50%), routine examination(32%). and pain(9%). 5. When considering the type of the cyst. lateral type is many most in Group I (71.4%) and central type is many most in Group Ⅱ(94.4%) and Group Ⅲ(100%) 6. The most size of dentigerous cyst was 2 crown size in Group I, 1 crown size in Group Ⅱ, above of 4 crown size in Group Ⅲ. 7. Almost involved teeth showed displacement and some tooth of displaced teeth showed delayed root development and dilaceration of root. 8. The most many response of alveolar bone was buccal bone expansion in Group I (67.3%). no bone expansion in Group Ⅱ(66.7%) and palatal bone expansion in Group Ⅲ (60.0%). 9. The percentage of involved teeth were as follows : The mandibular third molar was 31% and many most. The mandibular second premolar was 30%. Mesiodens of maxillary anterior area was 15%. The maxillary canine was 8%. The mandibular first premolar was 5%. 10. In the Group I, causes suggesting of dentigeous cyst are pulpotomized deciduous tooth(59.2%). severe dental caries of deciduous tooth, untreated traumatic history on the deciduous tooth etc. 11. The treatment method of dentigerous was marsupialization in 61.2% of cases of Group I and that was enucleation in 61.1% of cases of Group Ⅱ and in 80.0% of cases of Group Ⅲ.