Genetic Diversity of Korean Rice Breeding Parents as Measured by DNA Fingerprinting with Simple Sequence Repeat (SSR) Markers

Song,Moon-Tae,Lee,Jeom-Ho,Lee,Sang-Bok,Cho,Youn-Sang,Ku,Ja-hwan,Seo,Kyoung-In,Choi,Seong-ho,Hwang,Heung-Goo 한국자원식물학회 2003 Plant Resources Vol.6 No.1

Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Present study was conducted to evaluate the genetic diversity within a diverse collection of rice accessions used for Korean breeding programs. Two hundred eighty-seven rice cultivars, composed of temperate japonica, tropical japonica, indica, and Tongil-type of Korean crossing parents were evaluated by means of 15 simple sequence repeat (SSR) markers. A total of 99 alleles were detected, and the number of alleles per marker ranged from 4 to 11, with an average of 6.6 per locus. Polymorphism information content (PIC) for each of the SSR markers ranged from 0.2924 to 0.8102 with an average of 0.5785. These results, with the result that use of only 15 SSR markers made all rice cultivars examined could be uniquely distinguished, imply the efficiency of SSR markers for analysis of genetic diversity in rice. Cluster analysis was performed on similar coefficient matrics calculated from SSR markers to generate a dendogram in which two major groups corresponding to japonica (Group I) and indica and Tongil type rice (group II) with additional subclasses within both major groups. The narrowness of the Korean breeding germplasm was revealed by the fact that most of the Korean-bred and Japan-bred temperate japonica cultivars were concentrated into only 2 of the sub-group I-1 (143 cultivars) and I-2 (58 cultivars) among six sub-groups in major group of japonica. This is because of the japonica accessions used in this study was a very closely related ones because of frequent sharing of the crossing parents with similar genetic background with synergy effect of the inherited genetic difference between indica and japonica. A rice breeding strategy with the use of molecular markers was discussed for overcoming of genetic vulnerability owing to this genetic narrowness.

마늘 및 2,3-Dimercaptosuccinic acid의 投與가 姙娠白鼠의 카드뮴中毒에 미치는 影響에 關한 硏究

宋泰卜,裵恩相,廉容泰 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.1

Recently the garlic has been proved to have some protective effect on the heavy metal poisoning of cadmium and mercury in several studies. From this fact it may be assumed that the garlic would have a protective effect to the toxicity of heavy metals in pregnant rat also. Therefore this study was designed to confirm the effect of garlic on the toxicity of cadmium in the pregnant rat and its fetus. The result of it was compared with that of 2,3-dimercaptosuccinic acid (DMSA). The experimental groups were devided into 3 groups; cadmium-alone treated group, cadmium-garlic combined treated group, cadmium-DMSA combined treated group. Each group was composed of 3 sub-groups according to the dosage of cadmium injection. The total of 4㎎/kg, 8㎎/kg and 16㎎/kg of cadmium was injected subcutaneously at the back of pregnant rat in each subgroup for 4 days, from the 14th to the 17th day of gestational period. Garlic and DMSA were administered per os from the day before cadmium injection to the 21st gestational day. The results of this study were as follows: 1. The inhibition of fetal growth due to cadmium administration into pregnant rat was prohibited in cadmium-garlic or cadmium-DMSA combined treated group except for the 16㎎/kg cadmium injected subgroups. 2. The accumulation of cadmium in tissues of liver, kidney and blood of pregnant rat, placenta and fetus was increased according to the increase amount of cadmium injection in all 3 experimental groups. Garlic and DMSA decreased the cadmium levels of maternal liver, kidney and blood, but there was no significant difference of cadmium level between garlic and DMSA treated group. 3. Garlic decreased the accumulation of cadmium in tissues of placenta and fetus, but it was not significant statistically comparing with that of DMSA or no-treatment group. Correlations of cadmium levels among maternal blood, placenta and fetus were positively high. 4. Necrosis of proximal convoluted tubules with pyknosis of epithelial cells was observed extensively in fetal kidney of cadmium-alone treated rat. However, the necrosis of the tubules was found only in small numbers of the tubules in cadmium-garlic combined treated one, but there was no remarkable change in cadmium-DMSA combined treated rat comparing with cadmium-alone treated one. As a conclusion this study revealed that the garlic would reduce the accumulation of cadmium in maternal tissues and protect against the toxic effect of cadmium at fetus in context of the growth inhibition and histopathological damage of kidney, but would not attribute the interfering the transportation of cadmium through placental barrier.

자궁내 발육지연이 있는 출생체중 2,500 g 이상인 환아의 주산기 예후에 관한 연구

송태복,조명숙,하진아,윤대석,김철홍,김윤하,최영륜 全南大醫大 2004 전남의대학술지 Vol.40 No.1

캐릭터 지다인을 위한 다변량분석의 적용방안

정광태,송복희 한국기술교육대학교 2006 論文集 Vol.13 No.1

Character is one of the most important high value-added industries in 21 century. Various analyses on character are required to raise customer's satisfaction on character products. In this study, we proposed application method of factor analysis, cluster analysis, multi-dimensional scaling and application cases. From the factor analysis, aesthetic factors on character could be decided and image maps with aesthetic factors could be obtained. From the cluster analysis, characters could be grouped by its aesthetic characteristics. From the multi-dimensional scaling, a direction of character design could be decided. Finally, it is concluded that multi-variate analysis should be effectively applied to character design.

MathCAD를 사용한 X-선 회절상의 계산

조태경,송인명,권영준,이희복 공주대학교 사범대학 과학교육연구소 1994 과학교육연구 Vol.25 No.1

물질의 결정구조를 연구하는 데에는 일반적으로 X-선 회절상의 분석방법을 가장 많이 사용한다. 따라서 대학교육과정에서 고체물리학, 결정학, 재료과학 등 여러 분야에서 X-선 분광법에 대하여 공부한다. 그러나 실제 강의에서 X-선 회절의 이론을 X-선 회절상의 분석 방법과 직접 대응시켜 설명하기란 쉽지 않다. 본 논문은 MathCAD란는 software을 써서 X-선 회절 이론식으로 부터 간단하게 원하는 결정의 X-선 회절상을 계산하는 방법을 제시하고, X-선 결정구조 분석에 관한 학습과 연구에 활용할 수 있는 자료가 되도록 하였다. The most available technique for the study of the crystal structure is the analysis of X-ray diffraction patterns. For this reason lectures on X-ray diffractions have been given at universities in various subjects such as solid state physics, crystallography, material science, etc. However, it is not easy to teach the theory of X-ray diffraction in conjunction with the analysis X-ray diffraction patterns. In this study, we have used MathCAD software to calculate angles, intensities, and patterns of the X-ray diffraction of crystal using formula given in the theory without any complication. The program has shown in the study will be useful for research on crystallography as well as for teaching the subject.

부인과 감염질환 환자에서 Sparfloxacin의 임상적 효과에 대한 연구

변지수,송태복,강형근,김석모 최신의학사 1997 最新醫學 Vol.40 No.7

고용량 및 저용량 ticlopidine의 혈소판 응집억제 효과 비교: 예비연구

김제,이훈복,송희정,이태용,김재문 대한신경과학회 1999 대한신경과학회지 Vol.17 No.3

고온 초전도체 Y₁Ba₂Cu₃O_7-δ(CdO)_x의 핵사중극 상호작용 연구

전인,김복수,노태호,김영훈,서용문,송승기,박영민 명지대학교 자연과학연구소 2000 자연과학논문집 Vol.19 No.-

CdO가 첨가된 고온 초전도체 Y1Ba2CU3O7-δ(CdO)x(x=0.00,0.09,0.22,0.34,0.42,0.49)를 고상 반응법으로 제작하여 상온에서 Cu 핵사중극 공명 실험을 하였다. 구리-산소 평면에 있는 63Cu(2)의 핵사중극 공명 진동수는 31.13∼31.39 MHz이고, 65Cu(2)의 공명 진동수는 28.82∼29.06 MHz이었다. 이러한 공명 진동수는 χ가 증가할수록 높은 진동수 방향으로 이동하였다. 63Cu(2)과 65Cu(2)의 NQR 공명 진동수의 비는 모든 시료가 1.08로 63Cu과 65Cu의 핵 사중극 모멘트의 비와 같았다. 구리-산소 사슬에 있는 63Cu(1)의 공명 진동수는 22.15~22.36 MHz에서 관측되었는데, χ에 따른 공명 진동수의 이동 경향은 63Cu(2)과 65Cu(2)의 경우와 비슷하였다. 선폭은 63Cu(2)과 65Cu(2)그리고 63Cu(1)의 경우 모두 약 200∼300 kHz고, 전반적으로 χ가 증가할 수록 감소하였다. 스핀-에코 방법으로 구한 스핀-스핀 이완 시간 T2는 χ가 증가함에 따라 약간씩 증가하였다. We have studied Cu-NQR in CdO-mixed high-Tc superconductor Y1Ba2Cu3O7-δ(CdO)x(x=0.00, 0.09, 0.22, 0.34, 0.42, 0.49) at room temperature prepared by solid state reaction method. In the Cu-O plane, 63Cu(2)-NQR frequencies were measured to be in the range of 31.13-31.39 MHz and those of 65Cu(2) were in the range of 28.82-29.06 MHz. The position of spectrum-peaks shifted to the higher frequency with increasing χ. The ratio of 63Cu(2) and 65Cu(2)-NQR frequencies have the value of 1.08 which is equal to the ratio of 63Cu(2) and 65Cu(2) nuclear quadrupole moment for all samples. The resonance frequencies of 63Cu(1) in the Cu-O chain have been observed in the range of 22.15-22.36 MHz and the tendency of resonance frequency behaviour with χ were nearly the same for both 63Cu(2) and 65Cu(2). The line-widths of 63Cu(2), 65Cu(2) and 63Cu(1) were observed to the value of 200∼300 kHz and they decreased with increasing χ. The spin-spin relaxation time T2 obtained by spin-echo method increased slightly with increasing χ.

고콜레스테롤혈증 토끼에서의 지질강하효과와 독립적인 Naringin의 항동맥경화 효과

최성준,김효수,정태숙,복성해,박영배 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.11

신생아 제대혈 Leptin의 농도 : 성별, 출생 체중과의 관계 Concentrations Relationship to Gender and Birth Weight

최영륜,조창이,송태복 全南大醫大 2000 전남의대학술지 Vol.36 No.3