백서의 메칠수은 중독에 대한 마늘의 방어효과에 관한 실험적 연구

배은상,황정일,차철환 대한보건협회 1985 대한보건연구 Vol.11 No.2

일부 變異原性 농약의 經胎盤 효과에 관한 연구

裵恩相,金永煥,柳寅晟 대한보건협회 1993 대한보건연구 Vol.19 No.2

변이원성이 확인된 일부 농약 즉 dimethoate, dichlorvos, fenitrothion, trichlorfon, carbofuran, captafol 및 folpet, machete, pentachlorphenol을 선정하여 이들 농약이 태자에 미치는 세포유전 도성학적 영향을 규명하고서 이들 농약의 각각 LD50의 50%, 25%, 12.5%에 상당양을 mouse 임신 16일째에 복간내에 1회 투여하였다. Cole 및 Schmid의 방법에 준하여 태자를 적출하고 태자의 간세포(liver crythroblast)에서 소핵 출현 및SCE 빈도를 관찰하였다. 소핵의 출현율은(MNPCE/1000PCE) dicholrvos, fenitrothion, trichlorfon과pcp는 각각 LD50의 50% 투여군에서 각각 소핵출현율이 대조군에 비하여 3-4.8배의 증가를 보였으며dimethoate 및 carbofuran투여군에서는 변화가 없었다. 그러나 captan, captafol, folpet 및 machete 투여군의 경우는 각각 LD50의 50%와 25%군에서 무두 소핵출현율이 디조군의 2.2-4.6배(50%군), 2.0-3.3배(25%군) 증가를 나타내었다. 한편 SCE는 대조군에 비하여 1.5-2.4배 증가를 나타내었고 특히 captafo1 투여군의 경우 현저한 증가를 나타내었다. This reserch was carried out to investigate the transplacental genotoxic effects of some mutagenic pesticides in mice. As for pesticides, we used pesticides such as organic phosphate insecticides (Dimethoate, Dichlorvos, Fenitrothion and Trichlorfon), carbamate insecticide (Carbofuran), phthalimide fungicids (Caplan, Captafol, Folpet) and herbicides (Machete, Pentachlorophenol (pcp)). These pesticides was injected intra peritoneum of mice on the 16th day of gestation with 12.5%, 25% and 50% of LD50 respectively. Two fetuses per litter were extracted after twenty hours of injection, and micronucleated polychromatic erythrocyte(MNPCE) were observed in hepatic tissues, Six hours after injection, fetuses were extracted, and frequency of sister chromatid exchange(SCE) were measured after incubation of hepatic cells for 26 hours. The results of the experiment were as follows: 1. As for the Induction rate of MNPCE in fetal liver, 50% of LD50 treatment groups of Dichlorvos, Fenitrothion and Trichlorfon showed 3.0 to 4.8 times as high a rate as control(saline) group(p<0.05), Among 25% of LD50 treatment guorps, Fenitrothion only showed the higher rate than control guoup(p<0.05). Induction rates of MNPCE in the Dimethoate guoup and Carbofuran group increased slightly, but it was not statistically significant. In the Captan, Captafol and Folpet treatment guoups, all the groups which were treated by 50% of LD50 treatment, showed 2.0 to 4.6 times as high a rate as control(corn oil) group (p<0.01), and all 25% of LD50 treatment groups showed 2.0 to 3.3 times higher rate(p<0.05). Machete 50% and 25% of LD50 treatment groups showed 2.4 to 2.6 times as high a rate of MNPCE as control(corn oil) group (p<0.05), PCP 50% of LD50 treatment group also showed increased induction rate of MNPCE. 2. As for the average SCE frequency of fetal liver erythroblast, all the 25% and 12.5% of LD50 treatment groups showed 1.5 to 2.4 times as high as control(saline) groups except Dimethoate. Especially Captafol treatment guoup showed increased SCE frequency apparently(p<0.05).

불소주입 수돗물에 의한 알루미늄 용기의 용출특성

배은상 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.1

Aluminium is known to have neurotoxic effects. Aluminium compound can also be found in water and can be released from utensils during cooking. We have found that the leaching of aluminium from utensils is enhanced in the presence of trace quantities of fluoride ion. In an experiment conducted to estimate the rate of leaching, we have found that the presence of fluoride in water adjusted with pH 3∼pH 9 and boiled in an aluminium vessel, increased over 12 ppm of aluminium with in 10-40 min. Aluminium concentration was increased to increasing the surface-to-volume ratio of aluminium utensils and heating time. Leaching properties of aluminium ware increased to decreasing pH concentration.

環境變異原性硏究의 國際的 動向

裵恩相 高麗大學校 倂設 保健大學 保健科學硏究所 1981 保健科學論集 Vol.12 No.1

변이원성시험은 경제적으로 단기간에 많은 화학물질에 대한 유해성의 예측이 가능하므로 세계 각국의 각 분야에서 연구, 활약하고 있고, 보다 안전한 화학물질의 선별을 위하여 이미 십여 년 전부터 신규화학물질의 규제법으로 변이원성시험이 채택되어 있는데 반하여 우리나라는 이제 시작 단계에 있으므로 조속한 시일 내에 전문분야의 연구자들이 연구 활약하여 유해성물질의 선별 규제 및 안전성이 높은 대체물질을 개발하여 국민건강에 유의하여야 할 것이다.

Mouse 胎仔肝細胞에서 Benzene의 胎盤轉移에 의한 小核試驗

裵恩相,金永煥,金順德 高麗大學校 倂設 保健大學 保健科學硏究所 1987 保健科學論集 Vol.15 No.1

The potential genotoxic effect of benzene was studied by means of the micronucleus test in fetal mice liver at dosages ranging from 0.5㎖, 1.0㎖ and 2.0㎖/㎏. For the transplacental test pregnant mice(ICR) was used at 16 days of gestation and groups of mice were given one or two daily oral doses, and sacrificed 20hrs after once treatment, 6 hrs after twice treatment each dose of benzene. The results showed a significant increase in frequency of micronuclei was detectable in fetal mice liver at each dose of benzene. (Table 1)

SO₂개스가 植物成長에 미치는 實驗的硏究

裵恩相 高麗大學校 倂設 保健大學 保健科學硏究所 1970 保健科學論集 Vol.1 No.1

In order to find the So₂ gas concentration and time length needed to cause the leaf damage upon-Phleum pratense L., the author carriod out an experiment with an air-tight box, controlling the So₂ gas concentration for certain period inside it The seedlings tested were respectively three and eight days old, and two to three and five to six centimeters high. The result was as follows; 1. The seedlings under the So₂ gas concentration of 0.25 to 6.25 ppm, showed leaf withering and color change from the original green to lead-gray after 30 minutes to 8-hour exposure and to the white color after 24 to 48-hours exposure 2. The cell destruction and complete discoloration around the somoke-spots were found as shown in photo Ⅲ and Ⅳ.

一部 地下空間 粉塵의 突然變異原性 調査硏究

裵恩相,尹東蓮,金承南,金永煥 高麗大學校 倂設 保健大學 保健科學硏究所 1989 保健科學論集 Vol.16 No.1

This paper is to examine the mutagenic activity of the airborne particles at the sites of shopping center, parking lots, subway, tunnel in underground construction and atomsphe from May to December, 1988. For this examination, Ames method using Salmanella typhimurium was adopted. The results are as follows : 1. At the sampling sites of shopping center, parking lots, subway, tunnel and atmospher, the average concentrations of total suspended particles were 559.0u g /㎥, 4,475.0㎥/㎥ and 143.0ug /㎥, 374.5㎍/㎥, 442.0㎍/㎥, 4,475㎍/㎥ and 143.0㎍/㎥ respectively. 2. As for benzene and methanol extracts the concentrations were shopping center 21.6㎍/㎥ parking lots 53.4㎍/㎥, subway 46㎍/㎥ tunnel 372.0㎍/㎥ and atmospher is 165㎍/㎥ 3. The average revertant colonies of the benzene-ethanol extract of 250㎍ per plate on TA98 were shopping center 205/plate, parking lots 269/plate, subway 288/plate tunnel 407/plate, and atomsphere 549/plate. 4. The average revertant colonies of the airborne particles in 1 ㎥ air volume on TA98 were shopping center 20.4/㎥, parking lots 58.3/㎥, subway 55.0/㎥, tunnel 763.2/㎥, and atmospher 54.5/㎥. 5. Mutagenic activity of air at the sampling site of tunnel was higher than those of other sampling sites.

石炭 및 石油類 중 鉛 含量 調査

裵恩相,金永煥 高麗大學校 倂設 保健大學 保健科學硏究所 1980 保健科學論集 Vol.11 No.1

The combustion of fossil fuels may introduce some toxic elements into the atmosphere and more lead has been transferred to the air by use of TEL as an antiknock additive in gasoline. These substances have contaminated life support system such as food, air, water and soil large enough concentrations to produce acute or chronic illness. Our concern has focused on oils(bunker C, distillate fuel oil and gasoline) and coals in 17 mines which are located in Gangweon-do, Chungcheong-nam-do, Gyengsang-bug-do, Jeonla-nam-do, From oils and coals concentration of Pb were measured with atomic absorption spectrophotometer and results were analysed as follow. 1. Mean concentration for Pb in coals was 12.85㎍/g (7.5∼22.9). 2. Mean cocentration for Pb in crude oil was 1.8㎍/㎖, in bunker C oil 0.6㎍/㎖, in distillate fuel oil 0.5㎍/㎖, gasoline 0.47㎍/㎖. 3. Emitted amount of Pb from bunker C oil and gasoline in the year of 1990 in Seoul was estimated to be 22.04×10^(5)g.

우리나라 메밀의 一般成分

裵恩相,申東旿 高麗大學校 倂設 保健大學 保健科學硏究所 1983 保健科學論集 Vol.13 No.1

近來에 이르러 國民들의 食生活向上과 더불어 많은 사람들이 食生活 科學化를 위하여 식물성 단백질과 지방질의 식품에 관한 嗜好度가 높아지고 있다. 따라서 優秀食品으로 利用되고 있는 메밀의 主要成分 함량을 규명하고자 지역을 달리하여 蒐集된 시료를 분석하여 그 변이 정도를 밝힘으로써 食生活의 向上發展을 圖謀하고자 本硏究를 실시하였는데 그 結果를 要約하면 다음과 같다. 1. 一般成分의 分析結果는 蒐集地域에 따라 多少 차이가 있어서, 탄수화물의 함량은 67.4∼73.2%(그 중 당질은 63.5~70.5%), 단백질의 함량은 11.1∼13.9%, 그리고 지방 함량은 1.8∼3.2% 범주이었는데 平均的으로 보면, 경기지방 蒐集시료가 강원도지방 蒐集시료에 비하여 탄수화물 및 지방 함량은 높고, 단백질 함량은 낮았으며 종래의 분석결과와는 큰 차이가 없었다. 평균적으로 볼 때 지방 함량은 수수 및 옥수수보다 다소 떨어지나 단백질 함량은 기장, 옥수수, 조, 수수 등보다 다소 높았다. 2. 무기물함량을 보면 蒐集지역에 따라 Ca은 21~27㎎, P는 295∼340㎎, Fe는 2.0∼5.1㎎범주이었으며, 평균적으로 보면 P 함량은 강원도지역 蒐集시료에서 다소 높았고, Ca 함량은 수수, 기장, 옥수수보다 조나 밀이 현저히 낮았다. 3. Vitamin 함량은 蒐集지역에 따라 Vitamin B₁은 0.14~0.28㎎, Vitamin B₂는 0.09~0.14㎎, Niacin은 1.2∼1.9㎎범주이었으며, 평균적으로 보면 경기도 蒐集시료가 강원도 蒐集시료에 비하여 Vitamin B₁ 및 Vitamin B₂와 Niacin 함량이 다소 높았고, 또 이들 함량은 기장보다는 높으나 그 밖의 수수, 옥수수, 조, 밀보다는 떨어지는 경향이었다. Upon these days, concluded with improvement of eating habit and manner, people began to have deep concern about foods of vegetable, protein and fat to make sure scientific attitude to diet. Accordingly, in order to examine the content of important factor for buckwheat which has been used for excellent food and to analyze samples which have been collected in differential areas, I was an attempt to present limits of variation to assist improvement and development of eating habit and manner, and lastly this study has been turned into enforcement. Also, the result is summarized as follows. 1. The result of analysis for general ingredient gives little bit difference in ways of collecting areas. Thus, the result is shown as follows. First, in comparing with average values, carbohydrate contains 67.4∼73.2% (among them, glucose 63.5∼70.5%) with 11.1∼13.9 of protein content, fat content 1.8∼3.2 in the limits, and also in view of average, among the miscellaneous cereals of Kyunggi-do area comparably carbohydrate and fat content appears to be higher than that of Kangwon-do area, showing lower protein content. Further there was any big difference with result of analysis appeared in last days. To be compared with average, fat content is little bit lower than African millet, corn, howev.er, protein content appears to be little bit higher than Chinese millet, corn, foxtail millet, African millet, etc. 2. According to the analysis tests of mineral samples, in the variety of colleting areas. Calcium concludes 21∼27㎎ and phosphorus contains 295∼340㎎ and Iron, contains of 2.0∼5.1㎎ in the limits. To be compared with average phosphorus content is rather appears higher in miscellaneous cereals in Kangwon-do area, and to the apposit, calcium content, appears to be distinctively rather lower in foxtail millet or wheat compared with African millet, Chinese millet, corn. 3. The Vitamin contents appeared with in the limits of variety of collecting areas and it has in the limits of B 10. 14∼0.28㎎. Vitamin B2 with 0.09∼0. 14㎎, Niacin with 1.2∼l.9㎎. And to be compared with average, the miscellaneous cereals in Kyunggi-do appeared to be higher in Vitamin Bl Vitamin B2 and Niacin contents rather than miscellaneous cereals in Kangwon-do, and as well such contents were tents to lower rather than other African millet, corn, foxtail millet, wheat, even thought they are higher than Chinese millet.

Umu-test에 의한 di(2-Ethyl Hexyl)Phthalate와 di(n-Butyl)Phthalate의 變異原性 연구

배은상,김영환 高麗大學校 倂設 保健大學 保健科學硏究所 1997 保健科學論集 Vol.23 No.1

The SOS/umu-test in Salmonella typhimurium strain TA 1535/pSK 1002, a rapid system for detecting genotoxic agents, by monitoring SOS respons, was evaluated with di(2-ethyl hexyl) phthalate and di(n-butyl)phthalate. Umu gene expression could be defined on a basis of increased β-galactosidase activity by 2-fold over the background level. Results from the study show, that β-galactosidase activity appearance in DEHP and DBF was not higher with or without metabolic activation than the background level.