Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

소설의 재매개와 포스트휴먼의 형성-소설 ≪천룡팔부(天龍八部)≫에서 게임 ≪천룡팔부(天龍八部)3D≫까지-

최재용 ( Choi Jae-yong ) 한국중국어문학회 2017 中國文學 Vol.90 No.-

본고는 소설 《天龍八部》가 디지털 게임 《天龍八部3D》로 재매개(remediate)되는 과정을 추적하고, 그 과정 중에서 나타나는 미디어 판본의 차이를 밝힌다. 나아가 그 과정 속에서 새로운 주체, 즉 포스트휴먼이 등장하고 있다고 주장한다. This study starts with Jin Yong`s famous novel, < Demi-Gods and Semi-Devils >, or < Tianlongbabu >. But what I mainly discussed is not the novel itself, but a mobile game < Tianlongbabu3D >, which is based on the novel. I tried to find out what happened during the process of translating the novel into the game, and to explain what it means. One of my two main concept is `remediation`. When a new media emerges, people tend to think that this new media is going to completely replace the old one. But the invention of camera did not extinguish painters, and kindle did not replace paperback. In fact, picture and painting, e-book and actual book created a complex interaction, affecting each other. We call this interaction `remediation`. When a novel < Tianlongbabu > was transformed into a mobile game < Tianlongbabu3D >, this `remediation` happened. The game looks like it`s telling the same story as the novel did, but different media has different characteristic. For instance, in the game you can visit the same place as many times as you want, and beat enemies you couldn`t dream of beating in the novel. Next main concept is `posthuman`. By posthuman, I mean the change of the very concept of human being. To humanism subject, reason is very important. They have very clear `boundary`, which consists their `self`. But posthuman transcends above this boundary. They embrace `alien` things like metal arm or electric body parts, they do not exclude things that were considered to be outside of the boundary, like cyborg, robot or A.I.. I think what we see in the game < Tianlongbabu3D > is the emergence of posthuman. The playable character that the player controls is posthuman, and the player itself is also posthuman. Above the boundary of human/smartphone, the posthuman ascends. In short, through this study I found out that the remediation of novel by game produces new meanings and activities, and that the very concept of human self is shaking. Posthuman is already among us.

우심방을 침범한 거대 비호지킨씨 림프종 1예

강상범,진승원,이은경,박용현,최용호,김용주,박준철,김재형,홍순조,최규보,권종범,원용순,박건,이은희 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

농부가 아닌 환자에서 발생한 긴털가루진드기(Tyrophagus Putrescentiae)에 의한 기관지천식 1례

최대로,김형수,고창옥,김희선,윤호성,박용범,김상훈,이재영 대한천식 및 알레르기학회 2004 천식 및 알레르기 Vol.24 No.1

Storage mites are believed one of the most important allergens in farmers and rural regions. Tyrophagus putrescenitiae, belonging to storage mites, is found not only within storehouses but also in the house dust, and the third most common mites following Dematophagoides farinae and Dermatophagoides pteronyssnus in house dust in Korea. The sensitization rate to Trophagus putrescentiae was reported as high as that of house dust mite in patients visiting the allergy clinic in large cities in Korea. We experienced a 69-year-old man who had suffered from dyspnea, wheezing, and coughing for 10 years. He retired from the teaching profession, and had lived in a large city. Deajeon. The skin prick test showed a positive reaction to Tyrophagus putrescentiae and negative reactions to house dust mites. The bronchial challenge test with Tyrophagus putrescentiae showed late asthmatic reaction. We confirmed a Tyrophagus putrescentiae induced asthma in non occupational setting. (J Asthma Allergy Clin Immunol 24 : 141-5, 2004)

Flubendazole의 편충에 대한 구충효과

최원영,남호우,유채을,한인수,한경용 최신의학사 1988 最新醫學 Vol.31 No.7

MACHINING CENTER를 利用한 鏡面加工에서 表面粗度에 관한 硏究

최재용,전언찬 東亞大學校 海洋資源硏究所 1993 硏究論文集 Vol.6 No.1

This study has been performed to experiment of plastic metallic mold KP-4M on the variation of spindle revolution, feedrate, polishing times, grain size, and the pressure adding to tool.The results obtained from this study are as follows : 1. Surface roughness(Ra) grows worse, as the velocity is lower in the area of low revolution, and the velocity highter in the area of high revolution. 2. Above 1500rpm at the spindle revolution, Ra grows worse if polishing times (N) is less than 2 because of the preceding process, and the surface turn brillant if polishing times more than 4 because of loading. 3. As the higher pressure which adding to a tool is better when we expect more manufacturing quantity, and we need low pressure when surface roughness take precedence of quantity. 4. On the basic of the experimental findings, it is possible to be automatic of mirror like finishing with the machining center.

Pseudomonas aeruginosa 의 고온 충격 반응

최영길,최재연,채영규 漢陽大學校 環境科學硏究所 1991 環境科學論文集 Vol.12 No.-

최적의 성장 온도가 37℃인 Pseudomonas aeruginosa를 고온 충격(heat shock)을 주기 위하여 45℃와 48℃에서 각각 배양하였고, 이때 생성된 단백질들의 조성을 sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)를 이용하여 37℃에서 합성된 단백질들의 조성과 비교하였다. 실험을 수행한 45℃와 48℃에서 자란 균주가 37℃에서 자란 균주보다 적어도 3종류의 단백질(60kD, 68kD, 그리고 70kD)들을 더 많이 생성하였고 이들 단백질들은 고온에 의하여 생성된 잘못된 기능을 가진 단백질들을 세포내에서 제거하는 역할을 하고 있다. 같은 조건에 전사단계를 억제한 rifampicin 을 처리한 결과, 이들 단백질의 생성은 증가하지 않았다. 이상의 결과로 부터 45℃와 48℃에서 합성이 증가된 60kD, 68kD, 그리고 70kD의 단백질은 P. aeruginosa에서 고온 충격에 의해 전사단계에서 합성이 조절되는 고온 충격 단백질들임을 확인하였다. P. aeruginosa grows optimally at 37℃. When this strain has transferred to 45℃ or 48℃, the growth of the cell has been affected by the high temperature. Especially, some proteins(60kD, 68kD, and 70kD) were observed that those proteins were overexpressed to eliminate the malfunctional proteins, which were produced by high temperature in the cell. When the cell were grown at 45℃ or 48℃ with rifampicin, which is the transcription inhibitor, those effects were not observed. From these results, proteins produced from the culture conditions of 45℃ or 48℃ are clarified to be heat shock proteins which are regulated by the transcriptional level.

골프용품의 관여도수준과 상표충성도에 관한 연구

최종진,전용재 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The golf product industry needs to focus on the participation level to the product, which is a medium for the consumers to satisfy their diverse set of demands. This research purports to examine the effect of participation level on the level of brand loyalty at the time of purchasing golf products. The research subjects were selected among the customers of golf practice sites located in Seoul and Gyeonggi Province. The sampling method used for the research subjectsis the Convenient sampling method among probability sampling methods. The research subjects were asked to fill in the survey questionnaire in person in the form of self-administered method. Among the collected 220 survey questionnaires, 15 which were deemed unreliable in their content were excluded. As such, 205 questionnaires were used for the final data analysis. SPSS/PC statistical program 10.0 Version for Windows was used for data processing, and the following statistical methods were used. Firstly, Descriptive analysis was conducted to analyze the response distribution of research subjects' demographical variables and each question categories. Secondly, Cronbach' test was used for the reliability test of the survey questionnaire. Thirdly, independent T-test, One-way Anova Analysis, correlation analysis, and Multiple regression analysis are to be used for the testing of hypothesis, and Duncan test was used for the post-test. Statistically significant level was set at p<.05 level. The results of research conducted using the above mentioned methods are as follows. Firstly, the level of participation at the time of golf product purchase according to the golf product consumers' demographical variables, manifested difference in the product performance elements not corresponding to expectation when it comes to average monthly income, product performance elements when it comes to age, and elements of participation level at the time of golf product purchase in terms of educational level. However, there was no difference in the level of participation in terms of gender and occupation. Secondly, as for the level of brand loyalty at the time of golf product purchase according to the golf product consumers' demographical variables, there were differences in the importance of product and in brand perception elements. Also, there was difference in the elements of brand perception in it came to the educational level. However, no difference was shown in the level of brand loyalty for gender?ccupation, and monthly income level. Thirdly, the effect of participation level on the level of brand loyalty at the time of golf product purchase manifested significant influence of participation level and product performance elements on the elements of golf product loyalty level. Also, participation level and elements not corresponding to expectation seem to affect brand perception.

팥의 RAPD 분석 조건 최적화 연구

이충열,박현철,박진철,김성만,김용철,최재희,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

The object of this study was to optimize PCR condition for RAPD analysis in adzuki bean. The best template DNA concentration was 20ng(0.25unit taq polymerase and 2.5mM MgCl2), 40ng(1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2 and lunit taq polymerase and 7.0mM MgCl2), and 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, and 1unit taq polymerase and 4.5mM MgCl2) The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), 4.5mM(20ng template DNA and 0.25unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM(40ng template DNA and 1unit taq polymerase). Amount of taq polymerase was 0.25unit(20ng template DNA and 2.5mM MgCl2), 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, the best condition for PCR optimization was 60ng template DNA, 4.5mM MgCl2, and 1unit taq polymerase. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃; 90℃, 40℃, 72℃; and 92℃, 36℃, 72℃.

Consideration of Diluents Selection and Input Amounts of the Hunter Process for Tantalum Production

Jae‑Jin Sim,Sang‑Hoon Choi,Yong‑Kwan Lee,Sung Gue Heo,Taek‑Soo Kim,Seok‑Jun Seo,Kyoung‑Tae Park 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.7

Tantalum (Ta) is a heavy refractory metal with an atomic weight of 180.95 g/mol, a density of 16.6 g/cm3, and a high meltingpoint of 3017 °C. With its refractory characteristics, it shows excellent chemical and physical stability as well as corrosionand heat resistance at elevated temperatures. The demand for Ta metal and related compounds is increasing with the expansionof the electronics and chemical industries. The Hunter process was proven to be effective in producing Ta powder in1953. Hunter proposed a method in which potassium heptafluorotantalate (K2TaF7) was reduced by sodium (Na). Thus far,this process has been the primary commercial method to produce Ta powder. In this study, quantitative differences wereanalyzed for diluent selection. Additionally, consideration was given to changes in the caloric value depending on the inputamount of diluents. Finally, the optimum material input and the properties of the prepared Ta were analyzed. Stoichiometricratios of K2TaF7(1 mol), NaCl (6.2–6.7 mol), Na (5–7 mol) were weighed, to perform a metallothermic reduction reaction. After the reaction, the tantalum powder was recovered and the flush process was carried out. After that, it was dried in avacuum atmosphere. Physical properties such as oxygen concentration, PSA, ICP-OES, and XRD of powder were conductedto evaluate the characteristics of Ta powder that was finally manufactured.