호중구감소증 환자에서의 기관-기관지 아스페르길루스증 1예

권재철,송명준,김기조,오유경,김성용,박선희,최수미,이동건,이석,신완식,김춘추 대한감염학회 2004 감염과 화학요법 Vol.36 No.1

저자들은 급성 림프구성 백혈병 환자에서 항암치료 후 호중구감소증이 지속되는 상태에서 침습성 아스페르길루스증의 드문 형태인 치명적인 아스페르길루스 기관기관지염을 경험하고 문헌고찰과 함께 증례를 보고하는 바이다. Tracheobronchial aspergillosis is an uncommon clinical form of invasive aspergillosis, particularly found in patients with AIDS and in lung transplant recipients than in other immunocompromised patients. The rapidity of the disease progression can result in fatal airway obstruction in a short period of time that the patient may need emergency tracheostomy without knowing the cause of the obstruction. We describe a case of fatal tracheobronchial aspergillosis which developed in a 43-year-old female patient with acute lymphoblastic leukemia. Dyspnea and stridor developed on the 20th day after 2nd consolidation chemotherapy, which was during the prolonged neutropenic period. Airway narrowing was observed on the computed tomograph scan of neck and emergency tracheostomy was performed. Infiltration with aspergillus hyphae was found in the tracheal ring and bronchial mucosa. Despite the use of amphotericin B, the patient died of bleeding and airway obstruction.

국내 혈액질환 환자에서 침습성 아스페르길루스증의 구제치료로서 Voriconazole의 효과와 안전성

권재철,김시현,최수미,최재기,이동건,박선희,최정현,유진홍,신완식 대한감염학회 2010 감염과 화학요법 Vol.42 No.1

Background: Invasive aspergillosis (IA) is associated with significant morbidity and mortality in patients with hematologic malignancies. We investigated the efficacy and safety of voriconazole (VCZ) when used as salvage therapy for IA in Korean adults with hematologic malignancies who had not responded to prior antifungal therapy. Materials and Methods: We retrospectively reviewed data, collected from January 2007 to October 2008, from patients with proven or probable cases of IA. All were probable IA cases, except for one proven case. All cases were refractory or intolerant to antifungal therapy prior to administration of VCZ. Efficacy and safety were assessed in patients treated with VCZ for more than 3 days and for more than one dose, respectively. A favorable response [complete (CR) or partial (PR)] was defined by significant improvement of all clinical symptoms, signs, and radiologic abnormalities. Results: Fifty patients who met the inclusion criteria were enrolled. There were 27 male and 23 female patients with mean age of 44.4 years (range, 15-65 years). Underlying diseases were acute leukemia (35 cases), chronic myelogenous leukemia (4 cases), myelodysplastic syndrome (3 cases), lymphoma (3 cases) and other hematologic diseases (5 cases). Twenty-two patients had received chemotherapy and 13 patients had undergone hematopoietic stem cell transplantation. The lung was the main infection site (94%) followed by the sinus (6%). Amphotericin B deoxycholate alone was the most frequent previous antifungal therapy. The mean duration of antifungal therapy prior to VCZ therapy was 13.9±8.8 days (2-44 days). The median duration of VCZ therapy was 19 days (interquartile range, 49 days). Sixteen patients (32.0%) showed favorable responses (CR:PR=8:8) at the end of VCZ therapy. The numbers of patients with stable disease, progression and death were, 6 (12%), 6 (12%) and 22 (44%) respectively. Most of those with unfavorable responses had relapsed underlying malignancies or refractory graft versus host diseases. Twelve patients developed drug-related adverse events but only one patient stopped VCZ treatment prematurely. Conclusions: VCZ demonstrated an acceptable level of toxicity in patients with hematologic malignancies but further studies are required to prove its efficacy as salvage therapy.

Urechis unicinctus 난세포의 인공수정에 의한 구조적 변화

권혁제,김완종,신길상 순천향대학교 1994 논문집 Vol.17 No.3

The artificial insemination in Urechis unicinctus oocyte revealed some fine structural changes under electron microscope. The degradation of surface coat, retraction of microvilli, fusion of cortical granules with oocyte membrane are the events of fine structural changes after the fertilization. At fertilization, the cortical granules of oocyte are transported from the inner cortex toward oocyte membrane. After the fusion, the contents of cortical granules are discharged into the perivitelline space, the extracelluar space which in future develops into fertilization membrane. There are some indications that the fertilization occur on the microvillous tip in U. uicinctus. Under light microscope, one of the evidences on the fertilization was the formation of fertilization cone, which seemed to sperm entry site, on membrane of round mature oocyte.

고사리(Pteridium aquilinum)뿌리 전분의 이화학적 특성에 관한 연구 : 제2보 : 전분의 물리적 특성 Ⅱ. Physical Properties

曺哉銑,金成坤,李啓瑚,權泰完 경희대학교 부설 식량자원개발연구소 1981 硏究論文集 Vol.2 No.-

고사리뿌리 전분의 이화학적인 특성연구의 일환으로 실시된 물리적 특성시험 결과는 다음과 같다. 전분입자의 미구조의 결합력을 보기 위한 흡수도, 팽운력, 용해도 시험결과 감자전분이나 타피오카전분보다 강한 결합을 하고 있으며 곡류전분보다는 약하며 팽윤력이 single-stage pattern을 이루고 있었다. 가열에 의한 전분입자의 복굴절성 상실, α-아밀라아제에 의한 소화성 및 X-선 회절둥으로 전분의 호화과정을 살펴본 결과 55-60℃에서 호화가 시작되고 60~70℃에서는 95%이상 호화되었다. 또한 3~6%전분용액의 가열에 따른 점도변화를 amyl-ograph를 사용하여 실험한 결과 pasting temperature 62~68℃, 최고점도 80~840BU, 50℃로 냉각후의 점도는 110~555BU였다. 따라서 다른 전분에 비해서 점도가 낮으나 setback이 현저히 느렸다. 수분함량 50%의 전분겔의 경도는 팥 전분보다는 약하고 감자나 밀전분에 비해서는 현저히 높았다. 전분겔의 저장중 노화속도는 밀 전분보다는 느리고 파지오카 전분보다는 빨랐다. 고사리뿌리 전분은 팥 전분겔특성에 큰 변화를 주지않지만 노화를 억제하였고 밀 전분겔에 첨가시에는 겔의 경도가 저하되어 부드러워지는 경향을 보였다. The gelatinization phenomena of bracken root starch were examined by means of the loss of birefringence, degree of digestibility by amylase and X-ray diffraction. These results indicated that gelatinization temperature of the starch was 55∼60℃ and over 95% of starch were gelatinized at the temperature between 60 and 70℃. The swelling power of the bracken root starch was much less steeper than that of potato or tapioca starch. Amylograph data on the various starch concentrations showed the pasting temperature of 62∼68℃, peak height of 80∼840 Brabender unit (BU) and peak after cooling to 50℃ of 110∼555 BU. According to the information obtained from amylograph data, the bracken root starch showed low set back. The rate of retrogradation of the starch as tested by Texturometer was slower and faster than potato and tapioca starches, respectively.

뇌출혈이 합병된 감염성 심내막염 1례

박동건,이재욱,권삼,이동철,강승완 동국대학교 경주대학 1997 東國論集 Vol.16 No.2

감염성심내막염에 의한 신경계 합병증은 색전성 뇌경색, 색전성 뇌출혈, 두개내 패혈성 동맥류, 뇌농양, 뇌막염등 다양한데 항생제 요법의 발달과 판막치환술에도 불구하고 그 빈도는 감소되지 않고 있다. 이 중 두개내 패혈성 동맥류의 파열에 의한 뇌출혈은 사망률이 80%로 치명적일 수 있어 조기 발견 및 적절한 치료가 필요하다. 저자들은 두통, 열감, 근육통 등의 증상으로 내원하여 감염성 심내막염으로 진단받고, 항생제 치료로 임상증세의 호전중에 심한 두통과 전신적 발작이 있은 후 갑자기 발생한 두개내 뇌출혈을 심장초음파 및 뇌 컴퓨터 단층촬영을 통하여 진단, 경험하였기에 문헌고찰과 함께 보고하는 바이다. The complications of infective endocarditis may involve any organ system ie, cardia, neurologic, vascular, dermatologic, and kidney. Extracardiac complications are usually caused by either embolization of vegetations or deposition of immune complexes. Neurologic complications remain a significant problem in bacterial endocarditis and have been reported to occur in up to 20% to 40% of cases of infective endocarditis. The various types of central nervous system disease seen in patients with infective endocarditis are cerebral embolism (with infarction or with intracerebral hemorrhage), intracranial mycotic aneurysm, seizures, brain abscess, meningitis, mental change, psychiatric disorder. Mycotic aneurysms are uncommon but not rare and are an important cause of local or systemic sepsis and acute hemorrhage following aneurysmal rupture. Cerebral mycotic aneurysms have been noted in 2∼10% of cases of bacterial endocarditis and account for 2.5∼6.2% of all intracerebral aneurysms. We have experienced a case of neurologic complication due to infective endocarditis in a 23 year-old female patient, who admitted to our hospital because of fever, chill, headache, myalgia. This case was diagnosed by echocardiography, brain computed tomography. The patient died of sudden intracerebral hemorrhage in the 53th hospital day despite intensive medical treatment. We report one case of intracerebral hemorrhage complicated with infective endocarditis.

전립선암과 전립선비대증 환자에서 혈청 유리 및 총전립선특이항원 측정의 의의

서혁준,박형민,권태균,정성광,김법완,이재태 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Purpose: This study was peformed to investigate whether the free to total PSA ratio(F/T PSA ratio) offers a better discriminant for the detection of patient with prostatic cancer(CaP) and those with benign prostatic hyperplasia(BPH) than serum total PSA alone. Materials and Methods: From September 1996 to February 1999, free and total PSA levels were measured on frozen sera samples of 154 men with BPH and 46 with CaP using immunoradiometric assay. Results: In all subjects, the mean total PSA was significantly higher in CaP group(72.57±66.58ng/ml) than BPH group(3.12±4.10ng/ml). The mean PSAD was significantly higher in CaP group(1.64± 1.86) than BPH group(0.08±0.01). The mean F/T PSA ratio was significantly lower in CaP group(0.19± 0.07) than BPH group(0.30±0.13). Among 41 subjects whose total PSA were between 4-20ng/ml, the mean PSAD was significantly higher in CaP group(0.37±7.16) than BPH group(0.17± 0.07), but the mean F/T PSA ratio was not significantly different between CaP(0.19± 0.10) and BPH group(0.25±7.13). By comparing the sensitivity and specificity of total PSA alone, PSAD and F/T PSA ratio on receiver operating characterislic (ROC) Cutie in all subiects and subiects with total PSA between 4-20ng/ml, F/T PSA ratio had no superiority than total PSA alone and PSAD for discrimination between CaP and BPH. Conclusions: We suggest that the F/T PSA ratio is not superior to total PSA alone and FSAD in the detection of prostatic cancer, and further evaluation of the usefulness of F/T PSA ratio should be required.

전해산성수를 이용한 새로운 내시경 소독 시스템(Cleantop WM-1??)의 소독효율

이준행,이풍렬,송재훈,이남용,임윤정,장재권,김영호,김재준,백승운,이종철,최규완 대한감염학회 2001 감염 Vol.33 No.2

Background : Electrolyzed acid water (EAW) is a new liquid chemical germicide containing a mixture of oxidizing species and prepared by mixing a small amount of salt with tap water in an electrolyzer. We attempted an in-use evaluation of the high-level disinfection capability of Cleantop WM-1□, a new endoscope reprocessing system using EAW. Methods : Forty-seven flexible endoscopes were randomly collected just after upper endoscopic examinations and were disinfected using Cleantop WM-1□. Parts of the endoscopes without contact to EAW were disinfected with 75% ethyl alcohol. Immediately after the disinfection procedure, samples were taken from the biopsy channel (S-1), tip of the insertion tube (S-2), the umbilical cord (S-3), and the angulation knob (S-4). Results : In EAW-disinfected parts of the endoscopes, the culture-positive rates were 4.3% (2/47) in S-1 samples and 12.8% (6/47) in S-2 samples. In ethyl alcohol-disinfected area, the culture-positive rates were 2.1% (1/47) in S-3 samples and 25.5% (12/47) in S-4 samples. The colony counts of culture-positive samples ranged from 1 to 144. Pseudomonas aerugi-nasa was recovered from the angulation knob of an endoscope, but other contaminating organisms were mostly : normal flora or opportunistic pathogens. Conclusion : Although part of the endoscopes disinfected with 75% ethyl alcohol were contaminated with more organisms than EAW-disinfected areas, Cleantop WM-1□ showed a relatively good disinfection efficacy in reprocessing patient-used endoscopes. (Korean J Infect Dis 33:133∼138, 2001)

3,4,5-Trihydroxycinnamic Acid Inhibits Lipopolysaccharide-Induced Inflammatory Response through the Activation of Nrf2 Pathway in BV2 Microglial Cells

( Jae Won Lee ),( Yong Jun Choi ),( Jun Ho Park ),( Jae Young Sim ),( Yong Soo Kwon ),( Hee Jae Lee ),( Sung Soo Kim ),( Wan Joo Chun ) 한국응용약물학회 2013 Biomolecules & Therapeutics(구 응용약물학회지) Vol.21 No.1

3,4,5-Trihydroxycinnamic acid (THC) is a derivative of hydroxycinnamic acids, which have been reported to possess a variety of biological properties such as anti-inflammatory, anti-tumor, and neuroprotective activities. However, biological activity of THC has not been extensively examined. Recently, we reported that THC possesses anti-inflammatory activity in LPS-stimulated BV2 microglial cells. However, its precise mechanism by which THC exerts anti-inflammatory action has not been clearly identified. Therefore, the present study was carried out to understand the anti-inflammatory mechanism of THC in BV2 microglial cells. THC effectively suppressed the LPS-induced induction of pro-inflammatory mediators such as NO, TNF-a, and IL-1b. THC also suppressed expression of MCP-1, which plays a key role in the migration of activated microglia. To understand the underlying mechanism by which THC exerts these anti-inflammatory properties, involvement of Nrf2, which is a cytoprotective transcription factor, was examined. THC resulted in increased phosphorylation of Nrf2 with consequent expression of HO-1 in a concentration-dependent manner. THC-induced phosphorylation of Nrf2 was blocked with SB203580, a p38 MAPK inhibitor, indicating that p38 MAPK is the responsible kinase for the phosphorylation of Nrf2. Taken together, the present study for the first time demonstrates that THC exerts anti-inflammatory properties through the activation of Nrf2 in BV2 microglial cells, suggesting that THC might be a valuable therapeutic adjuvant for the treatment of inflammation-related disorders in the CNS.

S-401 DCA inhibits growth of a human anaplastic thyroid cancer via the p53 independent pathway.

( Jae Wan Kwon ),( Eon Ju Jeon ),( Eui Dal Jung ),( Ho-sang Son ) 대한내과학회 2016 대한내과학회 추계학술발표논문집 Vol.2016 No.1

Background: Targeting energy metabolism such as aerobic glycolysis can be a potential strategy for cancer treatment. Dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase (PDK), shifts the glucose metabolism from high-rate aerobic glycolysis to oxidative glucose metabolism, which regulates the proliferation of thyroid cancer cells. Methods: 8505C, an anaplastic cancer cell line, were grown in RPMI-1640 media containing 10% fetal bovine serum (FBS) with 1% penicillin and streptomycin in 37 °C with 5% CO2. MTT assay and Flow-cytometry were performed to check the cell viability and cell cycle status. Western blot analysis was performed to examine the expressions of genes involved in metabolic control, cell viability and apoptosis. Lactate measurement was performed by spectrophotometrically. Results: DCA enhanced production of reactive oxygen species (ROS), ROS involvement was confirmed by ROS scavenger N-acetyl-Cysteine (NAC) pretreatment, which customarily inhibited the effect of DCA alone. Cell viability assay demonstrated that DCA had selective cytotoxicity against human ATC cell line but not in normal cells as well as increased sodium/iodine symporter (NIS) expression. Flow cytometry and western blot showed that the apoptosis was followed by cell cycle arrest in G1 phase. Conclusions: DCA promoted induction of G1 cell cycle arrest and ROS production/intrinsic pathway of apoptosis in 8505-C. Furthermore DCA induced the expression of NIS. Taken together, DCA could be a useful candidate drug for treatment of anaplastic thyroid cancer.

Low concentration, multi taste detectable taste sensor using the high transconductance of a cascoded gated lateral bipolar junction transistor

Kwon, Jin-Beom,Jeong, Hyun-Min,Kwon, Hyurk-Choon,Lee, Sang-Won,Lee, Jae-Sung,Kim, Sae-Wan,Kim, Ok-Sik,Kwon, Dae-Hyuk,Kang, Shin-Won Elsevier 2017 Sensors and actuators. B Chemical Vol.248 No.-

<P><B>Abstract</B></P> <P>People are interested in eating good foods as a way of increasing their quality of life. Research conducted on the quantitative detection and characterization of the taste components of foods has received significant attention in recent years. However, taste sensors with higher sensitivities are needed because the expectations of the public are constantly increasing. In this study, a cascoded gated lateral bipolar junction transistor (C-GLBJT) was developed and used in taste sensor applications for detecting five taste substances. The results showed that the sensitivity of the C-GLBJT taste sensor was 0–2.55μA/decade, and it can detect very low concentrations (down to 1fM) of five taste solutions. The developed integrated C-GLBJT sensor could be able to provide more precise information about foods to consumers and patients who are interested in their well-being. Also, it may be of great help to advertisers and businessmen by providing them with reproducible quantitative information about their products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The cascaded gated lateral bipolar junction transistor (C-CLBJT) that has the high transconductance was developed and used in the taste sensor. </LI> <LI> The proposed taste sensor can detect five kinds of taste solutions which are very low concentration up to 1fM. </LI> <LI> Also, the proposed taste sensor has the sensitivities of 0–2.55μA/decade according to the types of lipid sensing membranes. </LI> <LI> The measuring capability of the proposed sensor is very large, and it can detect concentrations that are lower than the 1μM limit of human’s limit. </LI> <LI> Therefore, it can satisfy human expectations and may be of great help to enterprises through the quantification of the five kinds of tastes. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>