Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

노인의 넘어짐을 예측하는 Berg 균형 척도의 민감도와 특이도

이충휘,이현주 연세대학교 보건과학연구소 2002 보건과학논집 Vol.12 No.-

농촌형 급식교와 비급식교 중학생의 체조성 및 식행동 특성

이옥이,장현숙 한국 가정과 교육 학회 1999 한국가정과교육학회지 Vol.11 No.2

The purpose of this study is to investigate the body composition and the eating behavior of rural middle school provided with and without the national school lunch Program(NSLP) The subjects consisted of 34O rural middle school students provided with(n=177) and without(n= 163) NSLP. Data were collected by using a questionnaire. The results of this study were summarized as follows: 1. Anthropometric measurements were taken for body weight, height, triceps skinfold thickness and percentage of body fat. There was no significant difference in the height. body weight between girl students provided with (155.8cm. 47.2kg) and without (156.Ocm,49.7kg) NSLP. However, the height. body weight were higher in the boy students provided with NSLP(160.8cm, 50.4kg) than without (158.4cm, 48.4kg) NSLP. Percentage of body fat(%) were significantly higher in girl students provided without NSLP(27.5%) than in those with NSLP(25.0%). 2, Food habit scores of 68% of the subjects, regardless of with and without NSLP, were poor. However, appetite of 98% of the subjects, regardless of with and without NSLP, were very good. But rate of skipping meals and snack intakes of students provided without NSLP were significantly higher than in those with NSLP. In the case of instant food intakes. the students of eating three-four times per week were significantly higher in students provided without NSLP(41.1%) than in those with NSLP(20.9%)

한국판 Hamilton 우울증 평가 척도의 신뢰도, 타당도 연구

이중서,배승오,안용민,박두병,노경선,신현균,우행원,이홍식,한상익,김용식 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.4

Objectives : The reliability and validity of the Korean version of Hamilton Depression Rating Scale (K-HDRS) were examined in Korean patients depressive symptoms. Methods : 33 inpatients and 70 outpatients diagnosed as major depressive disorder or depressive episode of bipolar I disorder according to the DSM-IV criteria were assessed with K-HDRS, Clinical Global Impression score (CGI), Beck Depression Inventory (BDI) and Montgomery-Aberg Depression Rating Scale (MADRS). Results : Internal consistency (Cronhach's alpha coefficeint=0.76) and interrater reliability (r=0.94, p<0.001) were Statistically significant. Principal axis factoring analysis revealed 4 factors that accounted for 50.4% of the total variance. The Correlations of K-HDRS with CGI, BDI and MADRS were 0.84, 0.54, 0.58 respectively. Conclusion : These results showed that the K-HDRS could be a reliable and valid tool for the assessment of depressive Korean patients. The K-HDRS will be a useful tool for assessing depressive symptoms in Korea.

괴사후성과 비괴사후성 간경변증에서 원인별 주석교질 (^99mTc-tin colloid) 간스캔의 차이

이헌영,허승식,이강욱,정현용,김삼용,신영태,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

The differences of the liver scintigraphic features between postnecrotic and nonpostnecrotic liver cirrhosis were studied for 69 patients. Degrees of mottling and splenomegaly were no different between two groups but shrinked form was more frequent finding in postnecrotic type(23:10), and normal shape & enlarged left lobe with shrinked right lobe were relatively dominant in non-postnecrotic group(15:6, 10:2). Grade of extrahepatic uptake of ^99mTc-tin colloid was significantly higher in Child-Pugh B & C group than A group(p<0.05), but there was no difference between postnecrotic and non-postnecrotic groups. These will be important results on the dicision for the presence of cirrhosis according to the variable causes.

Inducing apoptpsis by the inhibition of c-myb in oral squamous carcinoma cell line, KB cell

Lee,Jung-Chang 대한구강생물학회 2007 International Journal of Oral Biology Vol.32 No.4

Oral squamous cell carcinoma (OSCC) is the most commonmalignancy and is a major cause of worldwide cancer mortality.The proto-oncogene c-myb plays an important role in regulationof cell growth and differentiation, and it is expressed at highlevels in hematopoietic cells and many other types of cancers.However, the function of c-myb is not well known in OSCC.The present study aimed to reveal the function of c-myb and totest the alternation of cell growth and signaling by c-myb inOSCC. In this study, c-myb and dominant-negatibe myb(DN-myb) were expressed in an adenovirus-mediated gene deliverysystem to KB cells. The over-expressed c-myb broughtincreased cellular proliferation compared with control cells.However, DN-myb infected KB cells showed significantreduction of cell growth and enhanced induction of apoptosis toactivate PARP and caspase 9. c-myb induced increase of IGF-I,-II and IGF-IR expressions while DN-myb down-regulatedthese expression. Activation of ERK and Akt/PKB pathwaywas shown only in c-myb transduced cells. These findingssuggest that the role of c-myb in cell growth of oral cancer cellsis partially mediated through the modulation of IGFs, ERK andAkt/PKB. From this results, DN-myb is strongly recommendedas a curable gene for the treatment of c-myb dependentmalignancies such as OSCC.

금자양중(가네코 카즈시게) 기증 박형 호의 복원 : -경질토기를 중심으로-

황현성,이해순,이용희 국립중앙박물관 2001 박물관보존과학 Vol.3 No.-

이번 가네코 카즈시게 선생이 기증한 여러 유물 중 金子124 수호는 약 60여개 정도의 파편을 접합하고, 자체 하중을 견딜 수 있는 접착제를 적절하게 사용하여 결손부분을 복원하였다. 또한 보존처리를 하는 동안에도 항상 가역성을 염두에 두면서 처리하였고, 색맞춤도 미관상 어색함이 없는 범위 내에서 처리하였다. 그 결과, 기형이 둥근 점토질의 박형(珀形) 항아리로 새롭게 탄생하였다. This article examines the conservation process of a donated Gourd-style jar (Object No. Geumja No. 124), which was broken into several fragments (more than 60) in the past. After assessing the condition of the object, each of the fragments were re-attached, and missing areas were gap-filled and re-touched. The authors discuss considerations, which were taken into account during the decision making process. These include the integrity of the object and the section of treatment materials, such as the reversibility of adhesives and filling materials. After its conservation, the authors believe that the object is in a better condition and ready for display in the future.

하악골에 발생된 Low grade 골육종:증례보고

조현이,정종철,김호성,최재욱,성대경,서지훈,김성범,이계혁 大韓顎顔面成形再建外科學會 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.1

Osteosarcoma is a primary malignant tumor arising most often in the long bone, but rare in jaw bone. Especially osteogenic sarcoma of the mandible is a rare tumor, comprising less than 0.5% of all head and neck tumors. Osteosarcoma occurs cheifly in young persons, and presenting very survival rates. Histopathologically this tumor can be classified three types, osteoblastic, fibroblastic, chondroblastic and classified from low grade to high grade by anaplasia or mitosis of the tumor cells. Sometimes, the exact diagnosis of osteosarcoma is difficult especially in low grade osteosarcoma, even if clinical or radiographical findings suggest to osteosarcoma. So suspcious to malignant bone tumor in clinical or radiological findings, biopsy should be taked from deep portion and multiple area, and sharing the patient history and radiographs with pathologist will assist in the development of the diagnosis. We report a case of low grade osteosarcoma on the mandible initially difficulty in accurate diagnosis. The patient visited our hospital for routine dental treatment but radiographic findings displayed ill-defined radiolucency with osteoid formation on the mandible. Final diagnosis was difficulty in initial biopsy but subsequent biopsy taked from deep portion presented infiltrative growth and mitosis of the tumor cell in some area with small osteoid bone formation, so we could reach final diagnosis as low grade sarcoma. We treated this patient with bloc resection of the mandible and immediate reconstruction with iliac corticocancellous block.

제브라피쉬에서 장신경계에 의한 장운동 조절

이명호,문현이,권영숙,맹학영,김철희 충남대학교 생물공학연구소 2011 생물공학연구지 Vol.17 No.-

Gerbera(Gerbera hybrida Hort)의 형태적 특징과 유전적 유연관계 분석

김현애,임현희,양원진,이재헌,이병영,이용문,권오창 동아대학교 농업생명과학연구소 2001 農業生命資援硏究 Vol.10 No.1

This study was conducted to analyze the morphological characteristics and genetic relationships among 24 species of Gerbera. Most of flower colors were pink. The numerical order of flower color was pink, orange, red, double-colored, and milk-white. Majority of flower types were single or semidouble flowers. A few species were double flowers. Flower diameters were from 7cm to 12cm, showed significant differences compared to other characteristics. Flower stalks were ranged from 55cm to 65cm. Only one species was the shortest as 55cm. The others were similar size as about 65cm. Main annual production yields were between 190 and 400 blossoms. Fifty seven reproducible polymorphic bands from eighty primers were used for analyses of genetic similarity. The genetic similarity of 24 collected Gerberas was largely classified into five groups. The average similarity coefficient was 0.72 ranged from 0.50 to 0.90. The highest similarity coefficient was shown between 'Sardana' with red/white flower color and double flower type, and 'Tamara' with orange flower color and double flower type as 0.90.