서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1997)

김재홍,문득곤,김정수,김용준,임동진,박상훈,김희성,이민수,송기훈,김갑형,김형석,성소영,이인섭,김석우,황지환,조창근,김경문,부태성 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

Background : In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. Objective & Methods : For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1997. 99 strains of N. gonorrhoeae were isolated, among which 45(45.5%) were PPNG. Conclusion : The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 45.5% in 1997.

Stented Graft의 임상경험

권태원,김호성,김도균,성규보,조용필,김건언 대한혈관외과학회 2000 Vascular Specialist International Vol.16 No.2

Purpose: Endovascular repair is a alternative treatment with favorable short-term result, but long term results are not available compared to standard operation. The authors have reviewed experiences of Asan Medical Center on stented graft, performed from Aug. 1998 to Feb. 2000, and the results as to age, operative indication, clinical condition, anatomic location, complication, follow-up are reported. Method: Seven patients who has received stented graft between Aug. 1998 and Feb. 2000 are reviewed retrospectively. Result: Of the seven patients, 6 were male and 1 was female. The mean age was 46.4 years (17~71). Indication for stented graft were two pseudoaneurysms, two iatrogenic arterio-venous fistulas, one thoracic aorta aneurysm, one abdominal aortic aneurysm, and one iliac artery occlusion with blue toe syndrome. Three different types of stented graft devices were utilized: Dacron-covered nitinol stented graft (made by the Asan Medical Center medical device team), Vanguard II (by Boston Scientific Corp.), and PTFE with nitinol stented graft (by Tae-woong). There was no perioperative mortality, and the technical success was achieved in five cases (71%). The patency at 14-month were 1009o (5/5). Two immediate complications are hemorrhage due to iliac & femoral artery avulsion requiring emergency bypass grafting, and deployment failure of stented graft requiring removal of retained graft device. Conclusion: Stented graft may be utilized even in complicated vascular situation but will need further development of device and more experience to prevent possible catastrophic complication. Indication should be decided by team approach and vascular surgeon should be prevent or participate during the procedure.

신피질 세포에서 organic hydroperoxide에 의해 유발된 세포사망

김근태 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

Background : Previous studies have shown that the hydrogen peroxide induces the cell injury through a lipid peroxidation-independent mechanism in renal epithelial cells. This study was therefore undertaken to examine the roles of lipid peroxidation in t-butylhydroperoxide (tBHP)-induced cell death to renal epithelial cells. Methods : Opossum kidney (OK) cell cultures, a renal tubular epithelial cell line, were used as the renal epithelial cell model. tBHP caused cell death in a dose-dependent manner over the concentration range of 0.05-2 mM. Results : The cell death was effectively prevented by the hydroxyl radical scavenger (dimethylthiourea), iron chelators (deferoxamine and phenanthroline), and antioxidants (DPPD and BHA), but not by the hydrogen peroxide scavenger catalase. tBHP caused a marked increase in lipid peroxidation, which was completely inhibited by DPPD and deferoxamine. tBHP-induced ATP depletion was prevented by deferoxamine and DPPD. Similar protective effects of antioxidants were observed in cell death induced by cumene hydroperoxide, another organic hydroperoxide. However, inorganic hydroperoxide (H2O2)-induced cell death was not altered by antioxidants. tBHP-induced apoptosis was prevented by DPPD and deferoxamine. Conclusions : These results indicate that organic hydroperoxide induces cell death through a lipid peroxidation-dependent mechanism, unlike inorganic hydroperoxide, in renal epithelial cells. 배경:신장 세뇨관 상피세포에서 inorganic hydroperoxide 인 H2O2가 lipid peroxidation-independent mechanism을 통해 세포 상해를 유도한다는 이전 연구 결과가 있었고, 본 연구에서는 신장 세뇨관 상피세포에 organic hydroperoxide인 t-butylhydroperoxide (tBHP)에 의해 유도된 세포사망에 있어 lipid peroxidation이 어떤 역할을 하는지 조사하였다. 방법 : 신피질 세포로는 Opossum Kidney (OK) cell이 이용되었다. tBHP는 0.05-2mM의 범위에서 농도 의존적으로 세포사망을 유도했다. 이러한 세포사망이 hydroxyl기 제거제인 dimethylthiourea, iron chelator인 deferoxamine과 phenanthroline, 항산화제인 DPPD와 BHA, 그리고 H2O2 제거제인 catalase에 의해 어떠한 차이를 나타내는 지를 관찰하였다. 결과 : 세포사망은 dimethylthiourea, deferoxamine과 phenanthroline, DPPD와 BHA에 의해 효과적으로 방지되었으나, catalase에 의해서는 방지되지 않았다. tBHP는 lipid peroxidation을 확연하게 증가시켰으며 이러한 효과는 DPPD와 deferoxamine에 의해 완전히 억제되었다. 또 tBHP에 의해 유도된 ATP 소모도 deferoxamine 과 DPPD에 의해 방지되었다. 항산화제의 이와 같은 방지효과가 다른 organic hydroperoxide인 cumene hydroperoxide에 의해 유도된 세포사망에서도 나타났으나, H2O2에 의해 유도된 세포사망은 영향을 받지 않았다. tBHP가 apoptosis를 유도하였으며, 이는 DPPD와 deferoxamine에 의해 방지되었다. 결론 : 이상의 결과는 신장 세뇨관 상피세포에서 organic hydroperoxide가 inorganic hydroperoxide와 달리 lipid peroxidation-dependent mechanism을 통해 세포사망을 유발함을 의미한다.

사람 말초 혈액 임파구의 interleukin-2 수용체(Tac 항원)발현 및 증식능의 조절에 대한 연구

김태근,조양자,정태준,김정목 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1

Proliferation of T lymphocytes has been determined by a threshold of signals generated by the interaction between interleukin-2 (IL-2) and its spedific membrane receptor (IL-2R). Regulation of IL-2R expression on the surface of lymphocytes is important in the generation of cytototocix T cells and lymphokine-activated killer (LAK) cells carrying anti-tumor effector functions in the body. To obtain the ideas in designing in vivo immunotherapy using biological response modifiers, this study examined the effects of the interferon(IFN)s, steroid, or colchicine on the IL-2R expression and proliferation of human peripheral blood lymphocytes(PBL). As incubation time was lengthened up to 72 hours, the number of Tac-positive cells and prolongation potential of human PBL upon stimulation wit IL-2 or mitogens were increased. IFN-α resulted in a significant inhibition of IL-2R expression induced by IL-2 but IFN-Υ had no effect. Colchicine or dexaamethasone also inhibited IL-2R expression and cell proliferation induced by IL-2 or mitogens. These findings suggest that IL-2R expression and proliferation of human PBL are inhibited by IFN-α, colchicine, or dexamethasone.

Streptococcus mutans의 치태형성에 대한 Leuconostoc lactis 51의 영향

김태근,양규호,오정석 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1

Dental caries is a bacterial disease of the dental hard tisssus, characterized by a localized, progressive, molecular disintegration of tooth structure. The action of Leuconostoc lactis 51 about plaque formation and replication by Streptococcus mutans was studied as follows. 1. Lower amount of plaque was produced at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone on the wires in the beaker. 2. Fewer cells of S. mutans were replicated at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone. 3. In M17Y broth, viable cells of S. mutans and L. lactis 51 increased for 12 hours, and decreased for 24 hours. In M17YS broth, viable cells of S. mutans showed time-dependent decrease at mixed culture of S. mutans and L. lactis 51. 4. The culture supernatant of L. lactis 51 didn't inhibit the replication of S. mutans and the formation of artificial plaque. 5. Sucrose and frutose were extracted from the culture supernatant of L. lactis 51 in M17YS broth. These results suggest that L. lactis 51 isolated from the oral cavity inhibits the replication of S. mutans and the formation of artificial plaque.

뇌자기공명영상촬영술상 병변을 관찰할 수 없는 허혈성 뇌경색

김진석,김태완,임정근 啓明大學校 醫科大學 1998 계명의대학술지 Vol.17 No.4

1. 임상적으로 뇌졸중이 의심되어 증상 발생후 24시간에서 2개월까지 Brain MRI을 실시한 722명의 환자들 중 결과가 가음성인 환자는 모두 22명 (3.1%)이었다. 2. Brain MRI상 병변은 관찰되지 않으나 임상 양상 및 신경학적 이상 소견으로 추정되는 병변의 위치를 구분해 보면 뇌간부가 13명 (59%)으로 가장 많았고, 피질하부 8명 (36%), 피질 1명 (4.5%)의 순 이었다. 3. 대뇌혈관 (large cerebral artery)폐쇄 보다는 분지 동맥 (branch artery)이나 소 관통동맥 (small perforating artery)의 폐쇄에 의한 것으로 추정되는 허혈성 뇌경색의 병변의 빈도가 높았다. 4. 병력, 뇌졸증 위험 인자, 임상 양상, 심전도 검사, 심초음파 검사 및 뇌혈관조영술등에서 혈전성 뇌경색은 12명 (55%), 색전성 뇌경색은 3명 (13%), 원인이 확실하지 않은 경우는 7명(32%)으로 혈전성 뇌경색의 빈도가 뚜렷하게 높았다. 5. T1강조 영상, T2,강조 영상 및 FLAIR sequence를 같이 실시함으로써 허혈성 뇌경색의 조기 발견은 과거의 연구 보고에 비하여 상당히 높아졌으나 계속적으로 Brain MRI상 가음성인 허혈성 뇌경색이 있음으로, 허혈성 뇌경색의 조기 진단에는 새로운 기법인 확산강조 자기공명영상, 관류강조 자기공명영상, 자기공명 분광경 등의 다른 특수한 Brain MRI이 필요할 것으로 생각되며, 항상 신경학적인 검사를 통한 임상적인 진단이 선행되어야 한다. Background : Brain magnetic resonance imaging (MRI) is more sensitive than computed tomography in the diagnosis of acute ischemic stroke, but may not visualize all acute ischemic stroke. For deeper understanding of MRI negative strokes, we reviewed case histories, abnormal neurological finding, and localized ischemic lesions presumedly which were not present in brain MRI scans. Methods : Patients with a clinically definite first ischemic stroke and absence of corresponding lesion in brain MRI scans were studied retrospectively with hospital records dating from January 1994 to November 1996 and prospectively from December 1996 to August 1997. Patient with transient ischemic stroke, postictal paralysis, central nervous system infection, demyelinating disease, hemiplegic migraine, functional or hysterical cause, multiple cerebral infarction, scans within 24 hours and equivocal findings were excluded. Brain MRI was scanned with T2WI, T1WI and FLAIR sequence. Results: We studied 722 patients with clinically diagnosed definite acute ischemic stroke during the study period and identified 22 patients (3.1%) with the absence of corresponding lesion in brain MRI scan. The ischemic strokes without brain lesions on MRI scan were clinically localized to the brain stem (n=13, 59%), subcortex (n=8, 36%) and in the cortex (n=1, 4.5%). Perforating arterial and thrombotic ischemic lesions seemed to be more common than large arterial & embolic lesions. Risk factors was not different between MRI positive and MRI negative ischemic stroke. Summary: Acute ischemic stroke with absence of brain lesion in MRI scans is more common in brain stem and subcortex than in the cortex. There is some potential limitation of standard MRI scan for diagnosis of acute ischemic stroke, therefore further scans with new imaging studies such as perfusion/diffusion weighted magnetic resonance imaging are required in selected cases.

精密測定 計器用 Stainless Steel의 諸性質에 미치는 熱處理의 影響

金善泰,安幸根,韓泰鍾 Natural Science Research Institute JEONJU UNIVERSI 1987 전주대학교 자연과학연구소 학술논문집 Vol.2 No.2

The changes of microstructure and hardness with the various heat treatments on the properties of Fe-13.36% Cr-0.37%C stainless steel for precision measuring gauge have been investigated. Type 420 stainless steel has the self-hardenability, because the nose of isothermal transformation diagram located greatly to the right. The highest hradness in the martensite martrix with undissolved carbide is obtained by oil and air cooling treatment after austenizing at 1000-1050℃, for 1/6-1/3hr. In case of low temperature tempering, internal stress is reduced without dissolution of martensite matrix, and hardness reduced slightly. In case of high temperature tempering, hardness is reduced greatly, because the martensite matrix is dissolved into a ferrite and carbide.

슬라이딩 평면을 이용한 I-PD 제어계의 구성

김성근,박건우,고태언 동의대학교 산업기술개발연구소 2001 産業技術硏究誌 Vol.15 No.-

In this paper, I-PD controller by nearal network is designed using variable structure controller. The I-PD control system is stable in the sense that the error is inside the constraint set, the variable structure controller is idle. If the error hits the boundary of constraint, the variable structure controller begins operation to force the error back to the constraint set. Applying this control technique to the position control system using a DC servo motor as a driver, the control performance of controller is verified by the results of computer simulation.

초기 소설사 이해의 시각

金槿泰 崇實語文學會 1997 崇實語文 Vol.13 No.-

Streptococcus mutans의 치태형성에 대한 Leuconostoc lactis 51의 영향

김태근,양규호,오종석 대한소아치과학회 2000 大韓小兒齒科學會誌 Vol.27 No.4

치아우식증은 치아구조의 국소적, 침윤적, 분자적인 붕괴로 특징지워지는 치아 경조직에 대한 세균성질환이다. 이런 치아우식증의 주 원인균인 S. mutans의 치태형성과 증식에 대한 아동의 구강에서 분리된 L. lactis 51의 작용을 연구하여 다음과 같은 결과를 얻었다. 1.비커 와이어 검사에서 S. mutans와 L. lactis 51의 혼합배양시 S. mutans 단독배양에 비해 치태의 무게가 감소하였다. 2.S. mutans는 S. mutans와 L. lactis 51의 혼합 배양에 비교하여 S. mutans 단독배양시에 생균수가 감소하였다. 3.S. mutans와 L. lactis 51은 M17Y broth에서 단독 및 혼합배양시 배양 12시간 때까지 증가하다가 24시간 때에 감소하였으나, M17YS broth에서는 S. mutans와 L. lactis 51의 혼합배양시 S. mutans의 생균수가 시간이 지남에 따라 감소하였다. 4.L. lactis 51의 배양 상청액은 S. mutans의 치태형성과 증식에 대해 억제 작용을 하지 못하였다. 5.M17YS broth에서의 L. lactis 51 배양 상청액 성분의 thin layer chromatography에서 자당과 과당이 계속 검출되었다. 이상의 결과를 종합하면 구강에서 분리된 L. lactis 51는 S. mutans의 인공치태 형성과 증식을 억제시키는 것으로 사료된다. Dental caries is a bacterial disease of the dental hard tisssus, characterized by a localized, progressive, molecular disintegration of tooth structure. The action of Leuconostoc lactis 51 about plaque formation and replication by Streptococcus mutans was studied as follow. 1.Lower amount of plaque was produced at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone on the wires in the beaker. 2.Fewer cells of S. mutans were replicated at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone. 3.In M17Y broth, viable cells of S. mutans and L. lactis 51 increased for 12 hours, and decreased for 24 hours. In M17YS broth, viable cells of S. mutans showed time-dependent decrease at mixed culture of S. mutans and L. lactis 51. 4.The culture supernatant of L. lactis 51 didn't inhibit the replication of S. mutans and the formation of artificial plaque. 5. Sucrose and fructose were extracted from the culture supernatant of L. lactis 51 in M17YS broth. Thest results suggest that L. lactis 51 isolated from the oral cavity inhibits the replication of S. mutans and the formation of artificial plaque.