선택적 신동맥 색전술을 이용한 신절석술 후의 신출혈 치료 1례

이중화,이보배,윤능수,임승수,박청희 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

A patient with uncontrollable postoperative renal hemorrhage was successfully treated by selective injection of gelfoam into the anterior inferior segmental branch of right renal artery. Follow up arteriography was done 16 days later. It revealed persistent occlusion of the bleeding vessels. This method is a simple effective procedure in cases of renal hemorrhage to diverse etiology without responsive management.

몰핀이 신경아세포종 SH-SY5Y 세포에서 Peroxynitrite에 의한 세포고사를 막는 것은 아편양 수용체나 Phosphatidylinositol-3 kinase (PI-3 kinase) 경로의 활성화에 의한 것이 아니다

정영표,이동렬,손용,김태요,윤재승,송윤강,김명선,박래길 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.2

Background: The effect of opioids on nitric oxide (NO)- and peroxynitrite-induced neuronal cell death is largely unknown. In the present study, we examined the effect of morphine on NO- and peroxynitrite-induced cell death using a human neuroblastoma SH-SY5Y cell line, which abundantly expresses μ, δ, k-opioid receptors. Methods: The cultured cells were pretreated with morphine and exposed to 3-morpholinosydnonimine (SIN-1) that simultaneously generates NO and superoxide, thus possibly forming peroxynitrite. The cell damage was assessed by using MTT assay ana crystal violet staining. Exposure of the cells to SIN-1 for 24 hours induced apoptotic cell death, as evaluated by the occurrence of morphological nuclear changes characteristics of apoptosis using 4', 6-diamidino-2-phenylindole (DAPI) and measurement of pro-apoptotic protease, caspase-3, activity. Results: Pretreatment of SH-SY5Y with morphine, significantly inhibited the apoptotic cell death in a dose-dependent manner. Morphine also inhibited SIN-1-induced proapoptotic protease, caspase-3, activity in a dose-dependent manner. However, naloxone (20 μM) hardly antagonized the effect of morphine in SIN-1-induced cell death. The selective ligands for opioid receptor subtypes, [D-Ala^2, N-Me-Phe^4, Gly-ol^5]enkephalin (DAMGO, μ-opioid receptor agonist), [D-Pen^2.5]enkephalin (DPDPE, δ-opioid receptor agonist) and U-69593 (k-opioid receptor agonist) at the concentration of 10 μM did not prevent the cell death induced by SIN-1. PI3-kinase inhibitors, Wortmannin and LY294002, did not inhibit the action of morphine on apoptotic cell death. The neuroblastoma cells treated with morphine significantly elevated glutathione levels (GSH). Conclusions: The present study showed that morphine protected human neuroblastoma cell line, SH-SY45Y, from the peroxynitrite-induced apoptotic cell death through elevated GSH levels. However, it is suggested that the elevation of GSH by morphine is not via the activation of opioid receptors and/or PI3-kinase pathway but via other unknown mechanism.

머리염색이 인체에 미치는 영향

윤형식,황성호,이현륭,김수호,박연석,권낙현,정호진,김동훈,노현주,홍성호,박병찬,이관,정해관 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.1

일상생활에서 모발염색은 흔히 접할 수 있는 미용의 한 종류로 특히 젊은층을 중심으로 폭발적으로 유행하고 있다. 염색을 위항 사용하는 약제는 표백제와 발색제 등 각종 화학약품이 사용되고 있으나 이로 인한 건강장해에 대한 연구는 그리 많지 않다. 저자들은 염색이 인체의 모발건강에 미치는 영향을 파악하기 위하여 염색과 관련된 주관적 증상과 모발의 변화에 대한 실험적 연구를 시행하였다. 동국대학교 경주 캠퍼스 재학생 80명을 대상으로 설문조사를 시행하여 염색 유 ·무 및 염색 후에 경험한 증상에 대해 설문 조사하였고, 의과대학 재학생 46명을 대상으로 피부 반응 테스트를 실시하였다. 또한 염색 전후의 모발 탄성도를 측정하였고 모발의 상태를 파악하기 위해 전자 현미경검사를 실시하였다. 설문조사 결과 염색 전에 비하여 염색 후 안구혼탁, 안구건조, 시력저하, 발진 및 접촉성 피부염, 모발손상, 모근손상 등의 증상을 더 많이 경험한다고 호소하였다(p<0.05). 모발손상과 모근손상은 헤어드라이어 사용 빈도에 따라 증가하는 것으로 조사되었다(p<0.05). 피부반응검사에서 가려움증이 가장 많은 증상이었으며 이는 여성보다는 남성에서 높은 것으로 조사되었다. 염색 전후의 모발장력은 염색 전 134.5±10.37g, 염색 128.0±30.69g, 염색 이틀 후 112.5±19.69g으로 나타났다. 염색 전후의 모발의 전자현미경 케라틴 층이 현저히 감소하고 모발이 가늘어지는 차이를 보였다. 염색은 모발손상, 모발 케라틴 손상 및 모근 손상, 발진 및 접촉성 피부반응, 안구혼탁, 안구건조, 시력 저하를 유발한다. 따라서 염색약으로 인한 손상에 대한 주의와 예방이 필요하다고 생각한다. 예방대책으로 염색 전 피부테스트를 통한 적합성 여부를 판단하는 것이 필요하며 가급적 염색을 피하는 것이 좋을 것이다. 염색약에 발암물질이 포함되어있다는 보고도 있어 염색 제조사의 철저한 실험과 염색 물질의 선별이 염색으로 인한 부작용을 최소화하는데 중요한 역할을 할 것이다. Hair coloring has became one of the most popular cosmetic activities to younger generations during last decade. However, there are few studies on the health effect of widespread use of chemical dyes. This study was conducted to study the effects of hair coloring dye on hair and other systems. We conducted a questionnaire survey of 80 persons in Kyongju campus, Dongguk University. We have done open patch skin test on 46 medical students. We also conducted scanning electron microscopy to examine the hair strength and structure before and after hair coloring process. Injury of hair and hair bulb, contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity were the main symptoms complained after hair coloring (p<0.05). Injury of hair and hair bulb were increased by frequency of hair-dryer use(p<0.05). In open patch test, pruritus was complanined by more than half of the subjects. Mean strength of hairs before and after hair coloring was as follows; 134.5 (SD 10.37)g before hair coloring, 128.0 (SD 30.69)g immediately after hair coloring, and 112.5 (SD 19.69)g after two days. The scanning electron microscopic findings of hair surface before and after hair coloring showed decreased keratin layer and thinning of the hair. Hair coloring induces injury to hair, its keratin layer, and hair bulb as well as contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity. Therefore, we think that precaution is needed in use of hair coloring dye. To prevent complications induced by hair coloring dye, it is necessary, especially to those with allergy or skin disorders, to perform skin test before action and avoid hair coloring whenever possible. Longterm health effects of hairdye should be studied and manufacturing companies should try to minimize complications induced by hair coloring dye.

양생온도변화에 따른 콘크리트의 강도 예측

박제선,김태경,이주형,윤청호 江原大學校 産業技術硏究所 1997 産業技術硏究 Vol.17 No.-

The maturity concept was adopted to predict the strength of concrete, which was subjected to several temperature levels and variable curing conditions. Penetration test and compressive test were conducted to measure the initial and final setting time and the compressible strength of concrete specimen, respectively. Also, the temperature and time were measured at some time intervals for calculating the maturity. The initial and final setting were delayed as the w/c ration increased and curing temperature decreased. The relationships at the relative strength and the equivalent age were proposed at different w/c ratio for the several temperature curing conditions, and these were applied for the variable curing conditions.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

Comparison of Everolimus- and Sirolimus-Eluting Stents in Patients With Long Coronary Artery Lesions

Park, D.W.,Kim, Y.H.,Song, H.G.,Ahn, J.M.,Kim, W.J.,Lee, J.Y.,Kang, S.J.,Lee, S.W.,Lee, C.W.,Park, S.W.,Yun, S.C.,Seung, K.B.,Yang, T.H.,Lee, S.G.,Lee, J.H.,Seong, I.W.,Cheong, S.S.,Lee, B.K.,Lee, N.H Elsevier 2011 JACC. Cardiovascular interventions Vol.4 No.10

Objectives: This study compared everolimus-eluting stents (EES) and sirolimus-eluting stents (SES) for long coronary lesions. Background: Outcomes remain relatively unfavorable for stent-based coronary intervention of lesions with long diseased segments. Methods: This randomized, multicenter, prospective trial compared the use of long EES with SES in 450 patients with long (≥25 mm) native coronary lesions. The primary endpoint of the trial was in-segment late luminal loss at 9-month angiographic follow-up. Results: The EES and SES groups had similar baseline characteristics. Lesion length was 34.0 +/- 15.4 mm in the EES group and 34.3 +/- 13.5 mm in the SES group (p = 0.85). Nine-month angiographic follow-up was performed in 80% of the EES group and 81% of the SES group (p = 0.69). In-segment late loss as the primary study endpoint was significantly larger in the EES group than in the SES group (0.17 +/- 0.41 mm vs. 0.09 +/- 0.30 mm, p for noninferiority = 0.96, p for superiority = 0.04). The in-segment binary restenosis rate was also higher in the EES group than in the SES group (7.3% vs. 2.7%, p = 0.046). However, in-stent late loss (0.22 +/- 0.43 mm vs. 0.18 +/- 0.28 mm, p = 0.29) and in-stent binary restenosis rate (3.9% vs. 2.7%, p = 0.53) were similar among the 2 groups. The incidence of any clinical outcomes (death, myocardial infarction, stent thrombosis, target lesion revascularization, and composite outcomes) was not statistically different between the 2 groups. Conclusions: For patients with long native coronary artery disease, EES implantation was associated with greater angiographic in-segment late loss and higher rates of in-segment restenosis compared with SES implantation. However, clinical outcomes were both excellent and not statistically different. (Percutaneous Treatment of LONG Native Coronary Lesions With Drug-Eluting Stent-III [LONG-DES-III]; NCT01078038)

Isolation and Identification of Indigenous Quorum Quenching Bacteria, <i>Pseudomonas</i> sp. 1A1, for Biofouling Control in MBR

Cheong, Won-Suk,Lee, Chi-Ho,Moon, Yun-Hee,Oh, Hyun-Suk,Kim, Sang-Ryoung,Lee, Sang H,Lee, Chung-Hak,Lee, Jung-Kee American Chemical Society 2013 INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH - Vol.52 No.31

<P>Recently, interspecies quorum quenching by bacterial cells has been reported as a novel approach to the biofouling control in a membrane bioreactor (MBR) for wastewater treatment. In this study, a novel quorum quenching (QQ) bacterium, <I>Pseudomonas</I> sp. 1A1, was isolated from lab-scale MBR and was encapsulated in a microbial vessel made of a hollow fiber microporous membrane for the biofouling control in MBR. <I>Pseudomonas</I> sp. 1A1 was most likely to produce AHL-acylase and degraded to a wide range of <I>N</I>-acyl homoserine lactones (AHLs), although there was a large difference in the degradation rate of each AHL. It has an extracellular QQ activity, i.e., it produces QQ enzymes, and excretes them out of the cell. The microbial vessel encapsulating <I>Pseudomonas</I> sp. 1A1 was applied to a lab-scale MBR and proved substantial inhibition of the membrane biofouling.</P>

Introduction to methodology for the development of an integrative medical service model

Cheong Moon Joo,Lee Myeung Su,Joo Min Cheol,Lee Sang-Yeol,Jung-Han Lee,Jong-Min Yun,Kang, Yeonseok,Lee Myeong Soo,Kang Hyung Won 한국한의학연구원 2022 Integrative Medicine Research Vol.11 No.2

Background: The purpose of this article was to present a valid and reliable theoretical framework and method for the development of an integrated medical service model for the four major diseases. Methods: Methodologies for each process were presented based on three theoretical frameworks. The three theories were the double diamond process model, the Donabedian model, and the International Classification of Functioning, Disability, and Health model. Based on this, in the process of problem finding, a literature review, service user questionnaire, and interview methods were applied. For problem definition and solution, expert focus group interview and expert Delphi methods were applied. The subjects of the study were mainly selected from experts for each disease and users of medical services. For literature review, systematic literature review and subject range literature review were conducted, and for analysis, a meta-analysis was carried out. In the case of focus group interview and Delphi, the validity and reliability of domain derivation and sub-items were analyzed by applying the qualitative analysis method. Results: To develop an integrative medical service model, a theoretical framework and a systematic methodology were needed, and case studies were performed to prove its validity and reliability. This showed that the development of a medical service model needs to follow a scientific procedure. Conclusions: By introducing methodological approaches and procedures for the development of an integrated medical service model for the four major diseases, the researchers provided basic information on model development.

Association of chronic viral hepatitis B with insulin resistance.

Lee, Jeong Gyu,Lee, Sangyeoup,Kim, Yun Jin,Cho, Byung Mann,Park, Joo Sung,Kim, Hyung Hoi,Cheong, Jaehun,Jeong, Dong Wook,Lee, Yu Hyun,Cho, Young Hye,Bae, Mi Jin,Choi, Eun Jung WJG Press 2012 WORLD JOURNAL OF GASTROENTEROLOGY Vol.18 No.42

<P>To investigate the relationship between chronic viral hepatitis B (CVHB) and insulin resistance (IR) in Korean adults.</P>

Relationship between the Regulation of Blood Pressure and in vivo Noradrenergic Neural Activities in the Locus Coeruleus of Young Spontaneously Hypertensive Rats

Lee, Eun Kyung,Ko, Kwang Ho,Jin, Chang Bae,Lee, Jin Hwa,Cheong, Jae Hoon,Kim, Yun Tai,Lee, Chung Jae 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.4

The purpose of the present study was to address whether the in vivo noradrenergic neural activities in the locus coeruleus are related to the development of hypertension. Two groups of the animals were prepared, 1) young SHR and 2) age-matched normotensive control, WKY. At the age of 6 weeks, blood pressure and the releases of NE and DOPEG from the locus coeruleus in young SHR and WKY were measured by in vivo microdialysis at two different conditions; 1) normal and 2) elevated state of blood pressure by systemically injected phenylephrine. Basal releases of NE and DOPEG from the locus coeruleus were 0.415 ±0.089 pg/20 min and 1.311 ±0.293 pg/20 min in SHR and 0.204±0.078 pg/20 min and 1.492 ±0.365 pg/20 min in WKY. The basal release of NE of SHR was significantly greater than that of WKY. Phenylephrine treatment caused elevation of blood pressure in both SHR and WKY in dose-dependent manner. Following phenylephrine injection, the releases of NE and DOPEG from the locus coeruleus of SHR were significantly decreased, whereas there was no significant changes of NE in WKY. The results from the present study suggests that the noradrenergic nervous system in the locus coeruleus may contribute as one of the triggering factors for the expression of hypertension in young SHR.