Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Son, Ji Yeon,Kang, Yoon Jong,Kim, Kyeong Seok,Kim, Tae Hyung,Lim, Sung Kwang,Lim, Hyun Jung,Jeong, Tae Cheon,Choi, Dal Woong,Chung, Kyu Hyuck,Lee, Byung Mu,Kim, Hyung Sik Korean Society of ToxicologyKorea Environmental Mu 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Ji Yeon Son,Yoon Jong Kang,Kyeong Seok Kim,Tae Hyung Kim,Sung Kwang Lim,Hyun Jung Lim,Tae Cheon Jeong,Dal Woong Choi,Kyu Hyuck Chung,Byung Mu Lee,Hyung Sik Kim 한국독성학회 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

Original Article : Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

( Ji Yeon Son ),( Yoon Jong Kang ),( Kyeong Seok Kim ),( Tae Hyung Kim ),( Sung Kwang Lim ),( Hyun Jung Lim ),( Tae Cheon Jeong ),( Dal Woong Choi ),( Kyu Hyuck Chung ),( Byung Mu Lee ),( Hyung Sik Ki 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

우리나라 상차림의 영양적 평가

김영순,이정희,오순덕,김수현,이정민,남택수,박태식,천유정 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.1

This study was carried out to investigate the current nutritional problems owing to the increased consumption of westernized food in Korea and also was conducted to compare the nutritional characteristics of Korean traditional meal with those of the Western diet in similar serving size. The results obtained from nutritional evaluation are as follows: 1. The percentage of calories represented by carbohydrate: lipid: protein was 65:15:20 respectively for Korean traditional meal (Table setting of 3 Chop) therefore these ratios meet the dietary recommended allowances for Koreans. 2. Nutrient values of Korean traditional 3-Chop were shown to be better balanced than those of Western diet when the nutrients of the two types of diet were estimated by similar serving sizes and calories. 3. Compared to the Korean traditional meal, most fast foods were nutritionally imbalanced especially in that most of them showed higher amounts of fats. These results show that Korean traditional meals supply ideal nutrient intakes in contrasts to the westernized diet, therefore much work remains in developing a variety of menus and standard recipes for Korean traditional meals according to the changes in diet trends as well as emphasing the importance of Korean traditional meals as part of an ideal, well-banlanced diet.

노인성질환 환자의 돌봄을 위한 전반적인 상태 평가표 및 돌봄을 이한 육체적 노동 요구 평가표의 타당도 및 신뢰도

김태유,김상윤,임병훈,권오영,최낙천 대한노인병학회 2002 대한노인병학회지 Vol.6 No.4

상악 4절치의 후방견인시 나타나는 현상에 관한 유한요소법적 분석

천옥진,김태우,서정훈 대한치과교정학회 1995 대한치과교정학회지 Vol.25 No.5

상악치열에서 제 1 소구치를 발거하고, 이 위치로 견치를 후방이동시킨 상태에서 retraction archwire를 사용하여 상악 4절치를 한 묶음으로 후방견인시켰을 때 초기에 나타나는 모든 힘 체계와 개개치아의 이동양상을 살펴보고 바람직하지 않은 치아이동을 감소시키기 위한 방안을 모색하고자 본 연구를 시행하였다. 먼저 유한요소법을 이용하여 상악 치아와 치근막의 해부학적 형태 및 생체적 특성을 컴퓨터로 재현시키고 모형화된 브라켓을 부착시킨 후, beam 요소를 사용하여 5종의 retraction loop를 하나씩 부여한 다섯 가지의 탄선을 모형화하고, 브라켓과 탄선간에는 gap 요소를 사용하여 모형화함으로써 전체 유한요소 모형을 완성하였다. loop의 형태, activation의 양, gable bend와 torque의 조건에 따른 차이를 검토하기 위해, 각 조건을 변화시켜 loop를 activation시켰을 때 탄선이 발휘하는 힘과 모멘트, 치아이동의 변위를 3차원적으로 정량적 및 가시적으로 평가하고 비교, 분석한 결과 다음과 같은 결론을 얻었다. 1. tear-drop loop archwire 사용시 각 치아의 근원심이동 및 설측이동이 발치공간 폐쇄에 도움이 되었으나, 바람직하지 못한 상하이동과 각 방향으로의 회전이동이 부가적으로 나타났다. 2. 다섯 가지의 retraction archwire 중 T-loop archwire에서 공간폐쇄에 도움이 되는 치아이동이 가장 작게 나타났으며, 각 방향으로의 회전이동 역시 가장 작게 나타났다. 3. tear-drop loop archwire에서 activation 양을 증가시킴에 따라 공간폐쇄에 도움이 되는 치아이동이 점차 증가했으나, 바람직하지 못한 상하이동과 각 방향으로의 회전이동이 함께 증가했다. 4. tear-drop loop archwire에서 loop의 전후방에 gable bend 부여시 전치에서 근원심방향의 치체이동에 도움이 되었으나, 모든 치아에서 상하이동과 각 방향으로의 회전이동이 함께 증가했다. 5. tear-drop loop archwire의 절치부에 치근 설측 torgue 부여시 이들 치아의 순설경사를 개선시키는 효과를 얻었으나, 그 반작용으로 견치 치관의 설측회전이 크게 증가했으며, 전치의 상하이동과 모든 치아에서 각 방향으로의 회전이동이 증가했다. This study was designed to investigate force systems and tooth movements produced by retraction archwire during retraction of four maxillary incisors after the maxillary canine retraction into the maxillary first premolar extraction space using the computer-aided three-dimensional finite element method. A three-dimensional finite element model, consisting of 2248 elements and 3194 nodes, was constructed. The model consisted of maxillary teeth and surrounding periodontal membranes, .022˝x.028˝-slot brackets, and 5 type of retraction archwires(.019˝x.025˝stainless steel archwire) modeled using the beam elements. The contact between the wire and the bracket slot was modeled using the gap elements because of the non-linear elastic behaviors of the contact between them. The forces and moments, and displacements produced by retraction archwire were measured at various conditions to investigate the difference according to types of loops, magnitudes of activation force, gable angle, and anterior lingual root torque. The results were expressed quantitative and visual in the three-dimensional method. The following conclusions can be drawn form this study : 1. When the tear-drop loop archwire was activated, the mesio-distal and lingual translational movements of the teeth helped to close the extraction space, but unwanted movements of the teeth including intrusions and extrusions, and rotational movements in each direction occurred. 2. Activation of T-loop archwire compared with those of other types of retraction archwires produced the least translational movements of the teeth helped to space closure and also least unwanted movements of the teeth. 3. Increasing amount of activation in the tear-drop archwire led not only to increase of translational movements of the teeth helped to space closure, but also increase if unwanted movements of the teeth. 4. Addition of gable bend in the tear-drop archwire helped anterior teeth to translational movements in the mesio-distal direction, but increased unwanted movements of the teeth. 5. Addition of anterior lingual torque in the tear-drop archwire helped central and lateral incisor to improve their facio-lingual inclination, but increased unwanted movements of the teeth.

養豚 排泄物의 好氣的및 嫌氣的 消化 處理에 關한 硏究 : A study on the Aerobic and Anaerobic digestion of the Swine Manure

김남천,김승곤,이태수 인천대학교 1992 基礎科學論文集 Vol.3 No.1

More than 95% reduction in BOD of swine manure treated with aerobic digestor can be obtained at F/M ratio of 0.06-0.143㎏·BOD/㎏ MLSS·day with 6.8 days hydraulic retention time. And in case of anaerobic digestor controlled at 35℃, more than 82% BOD removal can be obtained at VS loadings of less than 1.07㎏·VS/m³·day with more than 10 days sludge retention time.

만성적 소음폭로가 근로자의 혈압에 미치는 영향

김복연,김천태,이중정,박홍진,김창윤,강복수 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.1

The question of an association between occupational noise exposure and blood pressure has important public health implications. The harmful effects of hypertension are well known and noise is considered the most pervasive problem of all occupational exposures in Taegu City, Korea. A cross-sectional study on the effect of long-term noise exposure on blood pressure was done for 276 noise exposed workers(203 male workers, 73 female workers). Long-term noise exposure was measured by cumulative noise exposure level and noise-induced hearing loss. Hearing loss = 500Hz+2×1,000Hz+2×2,000Hz+4,000Hz -------------------------------------- 6 Questionnaire was administered to the workers, which includes age, sex, occupational history on the noise exposure, habits on alcohol drinking and smoking, practice of regular exercises, family history of hypertension. Type A behavior, question on stress, height, weight and blood pressure were measured. Serum triglyceride and low density lipoprotein(LDL) were also measured. Audiometry was done in the closed booth at 1,000, 2,000, 3,000, 4,000, 6,000, 8,000, 1,000 and 500Hz in order. Multiple logistic regression analysis revealed that cumulative noise exposure level(p〈0.05), serum triglyceride(p〈0.01), age(p〈0.01) and family history of hypertension(p〈0.05) predict hypertension (systolic blood pressure : 160㎜Hg or above, or diastolic blood pressure : 95㎜Hg or above) in male workers. In female workers, serum triglyceride(p〈0.01), LDL(p〈0.01) and family history of hypertension(p〈0.05) were predictors of hypertension (systolic blood pressure : 160㎜Hg or above, or diastolic blood pressure : 95㎜Hg or above). As the number of female workers with hypertension was too small, the multiple logistic regression was done according to hypertension criteria of systolic blood pressure greater than or equal to 140㎜Hg, or diastolic blood pressure greater than or equal to 90㎜Hg. Based on this criteria, cumulative noise exposure level(p=0.055) and age(p=0.057) predict hypertension. It is suggested that long-term noise exposure which was calculated by cumulative noise exposure level was a significant predictor of hypertension in noise exposed workers.

최대운동부하후 회복기중의 심박수·산소섭취량 변화와 유산소성 운동능력과의 관계 분석

김기진,박현,전태원 師範大學 體育硏究所 1988 서울大學校 體育硏究所論集 Vol.9 No.1

For the evaluation of cardiovascular function, aerobic capacity with VO₂max and AT(anaerobic threshold)have been used as a critical parameter. Also we expect that parameter during recovery can be used as a good one. The purpose of this study was to identify the relationship between maximal or submaximal aerobic capacity and the recovery rate of them. We studied with twenty seven subjects: 7 judoists, 10 long distance runners and 10 non-athletes. They were undergone a continuous incremented treadmill test from rest to all-out. Respiratory gas of each subjects was analyzed by using an automated respiratory gas analyzer, Ergo-Oxyscreen System (Erich-Jaeger Co.). As the data of heart rate, oxygen uptake at rest and during recovery made the log linear model with time, the gradient of recovery rate was calculated by the equation of simple regression with the time as independent variable. That equation is as follows; y=A+B.e?? ? y:HR or VO₂ ? A:expected value at rest ? B:maximum increment ? c:recovery rate The results of this study was; 1. There was a significant difference as the order of long distance runners, judoists, non-athletes' HR at rest, but there was no difference in the maximal HR. 2. Long distance runners had higher level of VO₂max(84.83ml/kg) and AT(80.03%) than the other two groups. 3. In the long distance runners group, the phase of VO₂recovery showed significant correlation with the other factors. 4. In judoists group, the phase of VO₂max recovery had significant correlation -0.79(p<0.05) with the maximal HR. 5. In all subjects, significant correlation was showed between AT, HR at rest and recovery of VO₂. In brief, maximal or submaximal aerobic capacity(VO₂max, AT etc.) had no correlation with the phase of HR recovery, but significant correlation with the phase of VO₂recovery in athletes. The results indicated that the recovery rate of oxygen uptake could be a critical index for evaluating the aerobic capacity.

총수담관결찰이 백서 신세뇨관의 S-100 면역반응에 미치는 영향

金天浩,李永浩,朴景蘭,申台均,金源式 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

For the study on the changes of S-100 reactivity at the renal tubular epithelium of rats(SpragueDawley, b.w. 250± 20gm. male), the common bile duct ligation was performed under ether anesthesia. At 1st, 3rd, 5th and 7th day after operation, the S-100 immunoreactivity were compared with control rats. The results were as followings; 1. In the control rat, S-100 positive reactions were observed at the collectiong tubule, Henle's loop, distal tubule(partly) and renal papilla. 2. In common bile duct ligated rats, S-100 positive reactions were observed at the proximal convoluted tubule in the rats 1 day after operation, and at the collecting tubule and proximal convoluted tubule in the 3 days after operation.