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심도식,이봉학,홍창우,김남윤,윤경구,김동호 강원대학교 석재복합신소재제품연구센터 1998 석재연 논문집 Vol.3 No.-
본 연구는 철강석의 정제후 부수적으로 발생하는 고로슬래그 미분말을 건설분야에 이용하여 고강도콘크리트를 제조하고 이에 대한 여러 가지의 역학적 특성을 알아보고자 하는 것이다. 실험의 주요인자로서 물/시멘트비, 굵은골재의 최대치수 및 시멘트의 종류로서 슬래그시멘트와 보통시멘트를 비교하였으며 제조된 콘크리트의 경화전 특성 실험으로는 슬럼프, 공기량, 응결시간을 측정하고 경화후 압축강도, 할렬인장강도, 휨강도 및 동결-융해 저항특성 실험을 수행하였다. 실험결과 슬래그시멘트와 19mm골재를 사용하고 물/시멘트비가 낮은 경우 높은 강도 발현은 물론 500cycle 이후에서도 상대동탄성계수가 90%이상을 나타내어 동결-융해에 대한 저항성이 매우 우수함을 나타내었다. 또한 슬럼프 경시변화에서도 보통시멘트를 사용한 콘크리트에 비해 슬럼프 손실률이 상당히 적어 유동성을 확보한 고강도 콘크리트의 생산이 가능함으로써 건설분야에 활용될 수 있을 것이다. The purpose of this study is to make high strength concrete using blast furnace slag and is to know the mechanical properties about it. Major variables of experiment are the water/cement ratio, coarse aggregate maximum size, portland and slag as cement type. After measuring of slump, air content and setting time about fresh concrete, compressive, tensile and flexible strength test and freezing-thawing resistance test also were carried out about hardend concrete. The results of test showed a good resistance to become higher than 90% of a relative modulus of elasticity after 506cycles and more strength in case of lower water/cement ratio and coarse aggregate of 19mm with slag cement. Also, concrete using blast furnace slag will make practical application of construction field to be high strength concrete, a small slump loss.
B16 melanoma 세포에서 nitric oxide가 멜라닌 생성에 미치는 영향
심상수,조남영,윤미연,이지윤,김창종 중앙대학교 약학연구소 2002 약학 논총 Vol.16 No.-
To investigate the effects of nitric oxide and cGMP on melanin production, we measured the melanin production in B16 melanoma cells stimulated by alpha-melanocyte stimulating hormone (MSH). NO donor, such as, sodium nitroprusside (SNP), SIN-1, SNAP and L-arginine increased melanin production in basal state. SNAP potentiated melanin production by MSH, but other NO donors did not. In the study the potentiating effect of sodium nitroprusside on cAMP-dependent melanin production, sodium nitroprusside did not increase melanin production induced by MSH, forskolin and 8-Br-cAMP. Non-selective cAMP/cGMP phosphodiesterase inhibitor (IBMX) and selective cGMP phosphodiesterase inhibitor (zaprinast) significantly increased MSH-induced melanin production, whereas soluble guanylyl cyclase inhibitor (ODQ) inhibited MSH-induced melanin production. These results suggest the possibility that MSH-stimulated melanin production may be mediated via not only cAMP-dependent pathway but also cGMP-dependent pathway in B16 melanoma cells.
RAW 264.7 세포에서 silica에 의한 유리 라디칼 생성에 미치는 protein kinase 억제제의 영향
심상수,조남영,윤미연,이지윤,김창종 중앙대학교 약학연구소 2002 약학 논총 Vol.16 No.-
To investigate involvement of protein kinase in reactive oxygen species (ROS) and peroxynitrite (PON) generation, we examined effect of protein kinase inhibitors on silica-induced ROS and PON generation in RAW 264.7 cells. Silica dose-dependently increased ROS and PON generation. Protein kinase C inhibitor, bisindolmaleimide and RO32-0432, slightly inhibited silica-induced ROS generation and significantly inhibited PON generation. Tyrosine kinase inhibitor, erbstatin and DHC, significantly inhibited silica-induced ROS generation but not PON generation. Calmodulin antagonist (W-7), calmodulin-dependent protein kinase Ⅱ inhibitor (KN-62) and mitogen-activated protein kinase inhibitor (PD98059) significantly inhibited silica-induced PON generation but not ROS generation. These results suggest that protein kinase C appears to be involved in both ROS and PON generation, tyrosine kinase in ROS generation, and calmodulin antagonist and mitogen-activated protein kinase in PON generation induced by silica in RAW 264.7 cells.
이심성,윤창주,김시중 聖心女子大學校 1981 論文集 Vol.12 No.2
수용액에서 Ag₂S 이온선택성전극의 특성은 잘 알려져 있다. 그러나 이 전극의 비수용매 또는 혼합용매계에서의 행동은 거의 알려진바 없다. 이 논문은 물-유기용매의 혼합용매계에서의 Ag₂S전극의 성질을 규명하려는 몇 가지 실험결과를 보이고 있다. 또한 Ag+이온의 선택성 용매화의 메카니즘에 관한 연구를 Ag₂S 전극을 이용하여 수행할 수 있음도 보이려고 한다. 할로겐화 이온 및 그들의 혼합물을 Ag₂S 전극을 사용하여 50%-MeOH용매계에서 분석하였다. 그 결과 은에 대한 전극 반응은 순수한 물에서의 것과 매우 유사하였다. The properties of Ag₂S ion-selective electrode in aqueous solvents well known. Very little is known, however, about the performance of this electrode in the systems of non-aqueous or mixed solvents. This paper presents the results of some experiments which is carried out to determine the properties of the silver sulfide electrode in water-organic mixed solvents. And it also shows the possibilities of studies on the selective solvation mechanism of silver ion by using the electrode. The halide ions and halide ion mixtures have been analyzed by potentionmetric titration using the electrode in 50% methanol system. As the result, the electrode response to silver ion in pure water and mixed solvent system is very similar.
이재영,윤성호,서유진,최정희,서창희,남동호,김윤근,민경업,박해심 대한천식 및 알레르기학회 2004 천식 및 알레르기 Vol.24 No.1
Background : The common whelk (Buccinum undatum) is one of the largest and most common snails in the North Atlantic. In Korea and Japan, common whelk is a popular eatable shellfish. Although shellfish has been known as the one of the most common causes of food allergy, there has been no published report on allergenecity and clinical significance of this sea snail. In this study, we determined the sensitization rate to common whelk and its relationship with other food allergens in allergy patients. Method : We carried out the skin prick test (SPT) with commonly consumed food stuffs in Korea including common whelk in 1700 patients over 1 year. The specific IgE to common whelk were detected by enzyme-linked immunosorbent assay (ELISA). ELISA inhibition test using sensitized sera was conducted. Results : SPT to common whelk was positive (≥2+) in 83 (4.9%) patients studied. Twenty-four (38.7%) of 62 SPT-positive patients had high serum specific IgE to common whelk. ELISA inhibition test showed significant inhibitions by abalone as well as by common whelk and, minimal inhibition were noted by shrimp. Significant correlation was also noted in specific IgE levels between common whelk and abalone (r=0.58, F<.05). Conclusion : IgE-sensitization rate to common whelk was 4.9% in allergy patients. Further stuides are needed to evaluate the clinical significance of sensitized patients to common whelk, and to confirm a cross reactivity with abalone. (J Asthma Allergy Immunol 24 : 77-84, 2004)
마크로-고리 화합물을 함유한 액체막의 특성에 관한 연구 : 칼륨 이온 운반에 미치는 음이온 효과
김시중,이심성,이선혜,윤창주 聖心女子大學校 1982 論文集 Vol.13 No.2
디벤조-18-크라운-6가 함유된 액체막을 통한 피크린산칼륨과 요오드화칼륨의 운반속도를 각 칼륨염의 농도 변화에 따라 측정하였다. 음이온의 종류에 따라 운반 메카니즘이 크게 다르게 나타나고 있다. 피크린산칼륨은 이온 확산에 가깝고, 요오드화칼륨은 이온 쌍 확산으로 운반되는 것 같다. The transport mechani는 of potassium picrate and potassium iodide across the liquid membrane containing dibenzo-18-crown-6 were studied by measuring the transport rate of the potassium salts. Potassium picrate seems to be transposted by the ionic diffusion but potassium iodide by the ion-pair.
박선영,조필교,이양심,윤인경,임양순,최창숙,장명희 한국 가정과 교육 학회 1998 한국가정과교육학회지 Vol.10 No.2
The purpose of the study is to develop a Performance Assessment Method & Tools for Home Economics at middle school. Through the content analyses of middle school Home Economics curriculum and 8 textbooks of lst, 2nd, and 3rd grade respectively, teching elements for each content area were drawn and each specific learning objectives were idntified accordingly. In addition, assessment element for student's performance in each teaching element were specified for analysis. Analytic framework for the development of Performance Assessment Method and Tools were proposed by each teaching element. Beased on the analytic framework proposed, a model method and tools were developed by 10 different test measures of description written test, statement written test, oral test, pro and con discussion, performance test, experiment, interview, observation, self-test report, and portfolio. The model proposed in the study has been modified and improved through the feasibility studies of experts' examination and field research application. The model assessment method and tools for the study has been compleeted finally by proposing 45 performance assessment tools for the content areas of food, clothing, housing, resource management and consumerism, human development and family relationship.