Apoptosis and necroptosis‑inducing effects of arctigenin on nasal septum carcinoma RPMI‑2650 cells in 2D and 3D culture

Yoon‑Jin Lee,Kwan‑Sik Park,Byoung Joon Baek,Kyong‑Ae Lee,Sang‑Han Lee 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Backgrounds Arctigenin derived from the seeds of Arctium lappa Linnaeus is known as an anticancer drug candidate by targeting various pathways involved in anticancer therapy. Methods Using 2D monolayer and 3D spheroid culture systems in nasal septum carcinoma RPMI-2650 cells, the effects of arctigenin and dexamethasone on cell viability, ROS levels, ATP level, mitochondrial function, apoptosis and necroptosis were examined. Results The combination treatment of both compounds induced strong cytotoxicity, accompanied by increases of sub-G0/ G1 peak, annexin V-PE-positive cells, and ROS levels, loss of mitochondrial membrane potential, and decrease of cellular ATP content. These changes were observed as simultaneous induction of DNA damage, apoptosis, and necroptosis. A series of changes by arctigenin and dexamethasone were efficiently restored by decreasing ROS levels or supplementing ATP. Treatment of 3D spheroids with arctigenin and dexamethasone decreased cell viability in the spheroids, but it was slightly resistant than cells under 2D conditions. In addition, this phenomenon was accompanied by an increase in mediators for both apoptosis and necroptosis. Conclusion Results of this study suggest that the apoptosis and necroptosis-inducing effects of arctigenin are associated with ATP depletion due to oxidative mitochondrial dysfunction.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

국내 민간투자(SOC)사업의 효율화 방안에 대한 연구

이호경,김상철,윤준선,백준홍 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

The purpose of this study is to examine the theoretical concept and practical example of Social Overhead Capital(SOC) project based on development of nation the world. Also, the critical access is to find the way we can develop domestic SOC project underway in private and nationwide. Furthermore, anther access is to propose clearly how to utilize BOT, BTO in financial, technical, CM method focusing on more systematic and effective result.

피나무(Tilia amurensis), 뽕잎피나무(T. taquetii), 일본피나무(T. japonica)의 형질분석을 통한 분류학적 한계

尹準九,張珍成,金輝,張桂羨,李興洙 서울大學校農業生命科學大學附屬樹木園 2003 서울大學校 樹木園 硏究報告 Vol.- No.23

본 연구는 한국산 피나무속의 3종(T.amurensis, T. insularis, T. taquetii)과 일본피나무(T.japonica)를 대상으로 6개 정량 형질(잎의 길이와 폭, 털의 밀도, 화경의 길이, 열매의 길이와 폭)을 비교 검토하였다. T.amurensis(피나무)와 T. insularis(섬피나무) T. taquetii(뽕잎피나무)는 기존에 잎의 크기와 털의 정도가 주요 형질로 알려져 있지만 본 연구결과 모두 뚜렷한 구분이 없는 연속변이를 보였다. 또한, 일본피나무와 국내 자생 피나무와 비교한 결과 기존에 알려진 화경의 길이에서 차이를 보였는데 제한된 개체를 근간으로 조사한 결과 일본피나무가 다소 긴 것을 확인하였다. 국내 자생 피나무, 섬피나무, 뽕잎피나무의 경우 독립된 종이기보다는 모두 피나무의 지역적 변이체임을 확인함과 동시에 동일종으로 처리하는 것이 타당하다고 생각한다. 그러나, 일본피나무의 경우는 좀 더 많은 개체수를 확보하여 화경의 길이에 대해 보다 자세한 조사가 이루어져야 할 것으로 생각된다. The hair density and type of leaf and bract, size of leaf and absence/presence of staminodes are important to recognize many taxa of Tilia. The systematic relationships among T. amurensis, T. insularis, T. taquetii in Korea and T. japonica in Japan were confused and were not clarified yet. Flower and fruit specimens of 121 individuals were sampled to investigate patterns of intraspecific variation and to evaluate the species delimitation among Tilia amurensis, T. taquetii, T. insularis, and T. japonica using six characters. The morphological differentiation among T. amurensis, T. insularis, and T. taquetii was not sufficient to warrant recognition of either specific or varietal status and should be treated as conspecific under T. amurensis. Based on results of limited number of specimens, the morphological discontinuity between T. amurensis and T. japonica was found only in peduncle length. Further study on this reproductive character are required to conduct any taxonomic treatment on these taxa.

Olanzapine 복용 이후 당뇨병 케톤산증이 발생한 정신분열병 환자

이중선,김진용,안준호,김창윤 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.1

The use of olanzapine has been associated with clinically significant hyperglycemia (exacerbation of existing diabetes, new-onset type 2 diabetes and diabetic ketoacidosis). We report a case of diabetic ketoacidosis (DKA) in 30-year-o1d male patient with schizophrenia who was on olanzapine. When he was admitted to the hospital because of DKA, he had been taking olanzapine for 43 months. 14 months before the admission, his fasting and 2 hour postprandial serum glucose levels were normal. He had no personal and family history of diabetes mellitus. He had no risk factor for diabetes mellitus except for obesity (body mass index : 39.2 kgW). He also hadno precipitating factors for DKA such as infection or myocardial infarction. Periodic monitoring for changes in serum glucose levels in patients taking olanzapine may be needed.

7075 Al 합금의 T9처리후 미세조직 및 인장강도 분석

이준희,송요승,김효열,이윤복 한국항공대학교 항공우주산업기술연구소 2002 航空宇宙産業技術硏究所 硏究誌 Vol.12 No.-

본 실험에서는 기존 텐트용 pole 로 사용되어 온 7075 AI의 T6처리 제품보다 더 우수한 인장강도 및 표면 특성을 갖는 제품 개발을 목적으로 T6처리에 냉간가공을 가미한 T9처리를 하여 그 특성을 알아보았다. 각 시편은 직경이 8.5㎜, 8.7㎜, 8.9㎜ 9.1㎜이고, 두께 t는 0.7 ㎜∼0.72㎜ 인 것을 사용하여 표면 조도, 경도 측정, 인장강도측정 및 조직관찰을 행하였다. 조도 측정 결과 T9시편이 T6시편에 비해 약 0.02㎛정도 작게 나왔으며 이것은 냉간가공에 의해 조직이 치밀해졌기 때문이라 여겨진다. 경도 측정과 최대인장강도 측정 결과 T9시편에 T6보다 큰 경도값과 최대인장강도 값을 가졌으며 이것은 냉간가공에 의한 grain의 심한 변형으로 인한 영향이라 여겨진다. 조직관찰 결과 T9시편이 T6시편보다 더 많은 변형이 일어난 것을 알 수 있었다. grain의 크기 차이가 많지 않았는데 이것은 열간가공과는 달리 냉간가공에서는 재결점 없이 단순히 grain의 변형에 따른 찌그러짐이나 분열에 의한 것이므로 grain의 크기에는 크게 영향을 주지 않았다고 사료된다. The properties of the samples after T9 treatment on 7075 aluminum which has been used as tent pole were studied in this paper. The T9 treatment is the process to be added a cold work after T6 treatment for improving the tensile strength and the properties of a surface roughness. The tests of surface roughness, hardness, tensile strength and micro structure on T9 treatment samples were conducted. The diameters of samples are 8.5㎜, 8.7㎜, 8.9㎜ and 9.1㎜. The thicknesses of samples are 0.7㎜ and 0.72 ㎜. The average surface roughness of samples after T9 treatment was less 0.02㎛ than that of T6 treatment The average values of hardness and tensile strength were increased after T9 treatment because of grain deformation.

원자력발전소 증기발생기의 저출력 수위조절의 자동화

이윤준 濟州大學校工科大學附屬産業技術硏究所 1991 尖端技術硏究所論文集 Vol.2 No.-

The steam generator water level is difficult to control at low power due to its reversed responses to the feedwater flow, which are well known as the shrink and swell phenomena. With regard to this problem a new control scheme has been studied by which the level transients could be kept within permissible ranges at low power. The relations between the various input conditions to steam generator and the level transients have been examined to be expressed in the form of process transfer functions. Analog filters have been incorporated to the process with proper control constants. This control scheme allows the prediction of level variation together with the corresponding feedwater rate and results in milder transients with good stabilities.

中醫治驗例를 中心으로 살펴본 癌性 疼痛의 外治法

李潤熙,申珉圭,卞俊晳 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1

Carcinous pain is severe and continuous so difficult to alleviate. Western drugs give rise to reation such as anoresia, constipation, voniting, general weakness and dyspnea, And they cause tolerence. External treatment is a way to put a patch or rub a liquid on the skin. The patch and liquid are made from herb medicine. In china, they have a good effect of alleviating carcinous pain to make use of external treatment. Usually they use the medicine with effect of reprecussion(消腫)removing obstruction(散結), activating blood flow and removing blood stasis (活血化瘀)such like Borneloum(水片). Bufonis Venenum (蟾?), Aconiti Ciliane Tubber(草鳥), Arisaematis Rhizoma(南星), Asari herba cum Radice (細辛)etc. We can get the alleviating effect more quickly and prevent reaction through extermal treatment.

골형성부전증 10예의 임상적 특징

이형숙,김현주,조재현,이승원,김현아,최준혁,송영준,김대중,이관우,정윤석 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.5

연구배경 및 방법: 골형성부전증은 비교적 희귀한 유전병으로 교원질 대사 장애로 인한 골의 취약성과 다발성 골절 및 척추측만증 등을 특징으로 한다. 유전방식과 표현형의 발현 정도에 따라 다양한 임상 양상을 보이며, 임상적 중증도에 따라 4가지 형태로 분류된다. 지금까지 국내 보고는 분만과정이나 태아 진찰시 골격이상으로 발견된 증례보고가 주였다. 저자들은 비교적 경미한 임상 양상을 보이는 예를 포함한 다수의 골형성부전증 환자들의 전반적인 임상적 특징에 대해 보고하는 바이다. 결과: 2001년 6월부터 2003년 2월까지 골형성부전증으로 진단받은 6 가계, 10예를 대상으로 하였다. 평균 나이는 27.3(5∼56)세였고 소아가 2예였다. 모두 상염색체 우성으로 유전되었으며, 제 I형이 4예, 제 III형이 4예, 그리고 제 IV형이 2예였다. 전 예에서 다수의 골절 경험이 있었고, 골밀도 저하와 골피질 두께 감소 소견이 관찰되었다. 전신의 평균 골밀도는 0.690(0.421∼1.039) g/cm²였다. 골형성지표로 측정된 sAlk는 소아의 경우만 증가되어 있었고, 골흡수지표로 측정된 uDPD의 평균치는 12.9(4.4∼36.3) nM/mM Cr으로 증가된 소견을 보였다. mobility score는 대부분 3,4단계에 속해 있었다. 중증형일수록 진단 시의 mobility score가 낮은 경향을 보였다. 결론: 한국인 골형성부전증 환자의 임상적 특징을 살펴본 결과 기존의 보고된 II형 외에도 I, III, IV형이 다양하게 존재함을 알 수 있었으며, 모든 예에서 상염색체 우성으로 유전됨을 확인할 수 있었다. 또한 모든 예에서 증가된 골흡수로 인한 골밀도 저하와 골절을 확인할 수 있었다. Osteogenesis Imperfecta (OI) is a relatively rare hereditary disease, which is characterized by multiple bone fractures and spine scoliosis, due to the fragility of bone, and is often associated with blue sclerae, deafness and dentinogenesis imperfecta. Four types of OI can be distinguished, according to the clinical findings. Although mutations affecting type I collagen are responsible for the disease in most patients, the mechanism by which the genetic defects cause abnormal bone development remains to be fully understood. Here, the clinical characteristics of 10 OI patient cases are reported, with a review of the literature. All the cases, including 4 type I, 4 type III and 2 type IV, inherited OI as an autosomal dominant trait. All the subjects had multiple old fractures and decreased bone densities. In this study, the biochemical marker of bone formation, serum alkaline phosphatase, was found to be increased only in the pediatric OI patients, while the biochemical marker of bone resorption, urinary deoxypyridinoline, was increased in all cases. The mobility score was found to correlate with the severity of the type on diagnosis (J Kor Soc Endocrinol 18:496∼503, 2003).