생체분해성 망막압정을 이용한 망막고정에 대한 실험적 연구

김용백,민병무,김창식,박근성,김승영,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Biodegradable retinal fixation devices obtain mechnical fixation of the retina with desirable chorioretinal scarring and with the potential for local, sustained release of antimetabolites and steroids to inhibit proliferative vitreoretinopathy. We manufactured a biodegradable retinal tack with barb that was designed in order to prevent intrusion from implantation of retinal tacks. This study was carried to evaluate the efficacy for retinal fixation and the capability for sustained release of drugs with a newly designed biodegradable retinal tack Biodegradable retinal tacks were made of polymers of glycolic acids and were designed with barbs in a shape to prevent the disinsertion. Biodegradale retinal tacks are divided into 3 parts, a conical portion that is inserted into the sclera, a cylinder portion that remains in the vitreous, and a neck portion between the pin and the cylinder. The tapered conical end was manufactured to allow easy insertion through the retina and choroid into the sclera. A cylinder portion was manufactured with a tapered angle that fixes firmly into the orifice of 19 gauge spinal needle. A neck portion, 0.4 mm in diameter, was designed to prevent disinsertion from following implantation of retinal tack. The applicator was a 19 gauge spinal needle and its orifice was prepared to 15°angle to accept the tapered cylinder portion of the retinal tack. The retinal tacks, secured in the needles, were passed through the formed vitreous and inserted into the retina, choroid, and sclera and were released by pushing the internal needle, usually within 2-3mm of the medullary ray of the posterior rabbit retina A retinal tack was placed in each of 8 pigmented rabbit eyes. Slit-lamp biomicroscopy, indirect ophthalmoscopy and fundus photography were performed periodically from 1 day to 8 weeks after surgery. Eight eyes were enucleated and studied by light microscopy at 8 weeks. Biomicroscopic evaluation of the animals revealed edemas adjacent to the retinal surfaces immediately after insertion of the biodegradable retinal tacks in all the animals. These edemas disappeared after 1 week. The first noticeable change in the size of retinal tacks was shown after 2weeks. The size of the retinal tacks gradually got smaller, decreasing to about one-half at 4 weeks and about one-third at 8 weeks. All retinal tacks remained in inserted places without any movement for an 8 week period. On light microscopy, epiretinal proliferations were seen to extend into the vitreous cavity. Cellular capsules that lined the inner aspect of the scleral defect caused by tack insertion were found. However the adjacent retina had a normal cytologic appearance and architecture in all specimens. We manufactured a biodegradable retinal tack that is designed to prevent intrusion from implantation of retinal tacks. All biodegradable retinal tacks reduce in size with time, but no retinal tacks extruded from the inserted place. The newly designed biodegradable retinal tack can be used for retinal fixation and may be used as a vehicle for the introduction of pharmacologic agents to prevent the cellular events that promote proliferative vitreoretinopathy.

피스톤 가공용 CNC 선반 HOT-1000 개발에 관한 연구

김경석,김종관,김상복,김성식,정현철,이도윤 한국공작기계학회 1996 한국생산제조학회지 Vol.5 No.2

The purpose of this study is the development of a lathe which can cut any pistons of oval shape with high speed, accuracy and precision by CNC (computer Numerical Control) method. Y axis which is the mechanism of position determination with high speed response has been aded to the CNC lathe and ovality and profile has been cut under the synchronous control of C-Y-Z axes. In the case of ovality of Φ0.5mm, the productivity can be improved up to 1500rpm by high speed cutting than modeling method.

한국인 알코올 의존 환자에서 알코올 대사 효소 유전자형 빈도의 남녀 차이

김성곤,김철민,이덕기,황인복,이현숙,김성연,전은숙,송영상,박제민,최병무,김명정 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.2

Objectives : There are a number of preceding epidemiological studies reporting gender differences in the genetic etiology of alcohol dependence. The author investigated gender difference in the frequencies of ADH2 and ALDH2 genoypes between the patients with alcohol dependence and normal control. Methods : The subjects were 141 alcohol dependent patients (104 males, 37 females) and 138 normal control (79 males, 59 females). The frequencies of 1/1 and 1/2+2/2 (2+ afterward) genotypes for ADH2 and ALDH2 were investigated in male and female between alcohol dependence and normal control group. DNA was extracted from WBC in peripheral venous blood and PCR-RFLP method was used out for genotyping. Results : First, the frequency of ADH2 1/1 genotype was significantly higher in alcohol dependent patients than normal control in both genders. Second, while there was no gender difference in the frequency of ADH2 1/1 genotype in normal controls, in the patient group however, the frequency was significantly higher in females than males. Third, in male subjects with alcohol dependence, the frequency of ALDH2 1/1 genotype was significantly higher than in male normal control subjects. On the other hand, in female subjects with alcohol dependence, the frequency of ALDH2 2+ genotype was significantly higher than in female normal control subjects. Conclusion : These results suggest that while the risk of alcohol dependence is predominantly affected by ALDH2 1/1 geno-type in male, the female ADH2 1/1 genotype is mainly associated with the risk of alcohol dependence. This means that there are gender differences in the genetic etiology of alcohol dependence.

경연 대회를 통한 초ㆍ중고생의 창의력 신장 효과에 관한 연구

이희복,육근철,류해일,김현섭,김희수,박달원,유병환,김선효,김여상,서광수,변두원,서명석,배성효,박종석,심규철,이성희 공주대학교 과학교육연구소 2001 과학교육연구 Vol.32 No.1

미래 사회를 대비하기 위한 개인의 개성을 존중과 창의적인 능력을 지닌 인간을 양성을 목적으로 충청남도 지역의 초 ㆍ 중고등학생들을 대상으로 한 창의력 경연대회가 공주대학교 과학교육연구소에서 개최되었다. 충청남도 초 ㆍ중 ㆍ 고등학생들을 대상으로 실시한 창의력 경연대회는 창조적 아이디어를 내어 스스로 문제를 해결해 나갈 수 있는 교육 프로그램을 제공하는 좋은 기회였음이 확인되었으나, 일부의 팀에서만 참신하고 재미있는 아이디어들이 제안되었다. 여학생의 참가자 수가 상당히 증가하였으며, 대체적으로 입상권의 학생들의 상당 부분이 여학생이라는 것이 특이할만하다. 학년이 올라감에 따라서 창의적인 아이디어를 내는 양이나 질에 있어서 뒤떨어지는 것으로 분석되었으며, 토론 학습의 적응력이 미흡하고 발표력 및 청취력에 있어서도 떨어지는 것으로 나타났다. 따라서 중 ㆍ고등학교의 학교교육에서도 학생들에게 프로젝트형 탐구학습 프로그램의 적용은 물론 토론식 수습을 통한 학생들의 사고의 전환의 기회를 제공해야 할 것으로 사료된다. The creativity competition was held to foster the creativity of elementary and secondary school students in the Institute of Science Education, Kongju national University. It was found that the creativity competition effected on the improvement of inventing creative ideas and problem solving activities. But, only some competitors participated in semifinal and final contests, presented original and interesting ideas. More female students participated in competition than last year. More female participants were awarded a prize than males. Totally, participants were defective in discussion and communication, and presentation and listening ideas. Increasing grades correlated inversely with creativity and originality. There is need of inquiry project teaming programs and presenting opportunities of conversion of thinking by discussing instructions in school

간호실무에서 신봉이론(Espoused theories)에 대한 탐색적 연구

서문자,김혜숙,이은희,박영숙,조경숙,강현숙,임난영,김주현,이소우,조복희,이명하,지성애,하양숙,손영희,권성복,김희진,추진아 성인간호학회 2001 성인간호학회지 Vol.13 No.1

As a nursing practice involves nurses' actions in a specific context of health care, this study has focused on exploring the espoused theories in nursing practice within the action science perspectives, Espoused theories are the belief, principles, and rationale expressed by the practitioner as guiding her/his actions in a situation of practice. The data were analysed qualitatively and 25 elements of espoused theories of nursing action were identified and clustered into 6 categories. The 25 elements of espoused theories are as follows: The clinical nurse worked in wholistic and individual nursing, focussed on the patient's needed, comfort and supportive nursing (5 theories of nursing goal) ; excellent skills, knowledge based, assessment and data collection, explaining, educating or a scientific basis(6 theories of nursing intervention): advocacy, value oriented, treatment, account- ability and commitment(4 theories of nursing ethics) ; human respect. partnership, trust(3 theories of patient-nurse relationship) : knowledgable, accumulated clinical experiences and personally lived experiences. positive perspectives(4 theories of nurse), role of intervention. rewarding peer relationship(3 theories of situations) The above mentioned espoused theories are similar to that of nursing textbooks which students learned through basic nursing education and almost the same as the Acts ofa Nurse in Korean. However, we are doubtful whether nurses actually do as they think. Therefore. it is recommended to review the theories-in-use in order to and any discrepancies between the espoused theories and the reality of nursing actions

만성 심부전 환자에서의 Denopamine 투여효과

최석구,김현대,정태기,김대현,김용복,유원상 인제대학교 1994 仁濟醫學 Vol.15 No.2

만성 심부전 환자에게 새로운 경구용 비 digitalis 강심제인 denopamine 1T(5mg)을 1일 3회 3개월간 투여하여 그 유효성 및 안전성을 검토했다. Background : Patients with congestive heart failure have a high mortality rate and are also hospitalized frequently. We studied the effects of a new oral inotrophic agent, denopamine in patients with chronic heart failure. Methods : 16 patients receiving conventional treatments for heart failure were treat ed with denopamine 5mg tad for at shortest 3 months. Their history were taken and the physical examination, general laboratory test and the echocardiographic examination were done before and after treatment. Results: There were 3 deaths and 1 withdrawal because of worsening heart failure. 5 patients (42%) among 12 patients showed the improvement in their subjective symptoms. The fractional shortening and ejection fraction of the left ventricle were increased by 3.2% and 5.6% respectively in their absolute values. Conclusion : The addition of denopamine to conventional therapy considerably improved the subjective symptoms and the left ventricular function in the limited number of patients with chronic heart failure.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

자궁경부암에서 H-ras의 재배열과 v-mos의 증폭

김재형,함덕상,고복현,김대광 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

Uterine cervical cancer is the most common cancer of woman in Korea. To understand which oncogenes are involved in squamous cell carcinomas of the uterine cervix, author examined nine human tissues of squamous cell carcinomas of the uterine cervix using Southern blotting techniques for five known oncogenes (H-ras, v-mos, erbB2, c-myc, K-ras). One of the nine tumors demonstrated a rearrangement of the H-ras oncogene. And amplification of v-mos was found in three tumors. The other oncogenes(erbB2, c-myc and K-ras) were the same pattern with the control. It is presumed that rearrangement of H-ras and amplifications of v-mos may be concerned to oncogenesis of squamous cell carcinomas of uterine cervix.

Estradiol-17β를 처리한 흰쥐 자궁에 있어서 Clomiphene이 세포내 수용체, DNA 및 cAMP 농도에 미치는 영향

김곤섭,고복현 東國大學校 경주캠퍼스 2007 慶州大學論文集 Vol.8 No.-

미국의 식물유래약용물질(Plant-Made Pharmaceuticals, PMPs)의 안전성 규제

김태산,원소윤,이근표,류태훈,진용문,이길복,김현준 한국국제농업개발학회 2005 韓國國際農業開發學會誌 Vol.17 No.1

1. 식물유래 약용물질 (PMP)은 살아있는 식물에서 생산된 치료용 단백질로, 발효조에서 세포계통을 이용하는 것보다 적은 비용으로 높은 순도의 단백질을 생산할 수 있어 이에 대한 연구개발이 확대될 전망이다. 2. 식물유래 약용물질의 재배시험을 감독하는 주관부서는 미농무부(USDA)의 동식물 검역청(APHIS)이다. 3. PMP의 생산에는 알팔파. 옥수수, 벼 잇꽃과 담배 등이 이용되고 있다. 4. 현재 상업화된 PMP는 없으나 ProdiGene, Inc사 등 몇몇 생명공학 회사에서 이들의 연구개발이 진행되고 있다. 5. 2002년에 PMP 재배시험은 130에이커 규모로 34개 시험장에서 20건이 허가 되었으며 주별로는 애리조나, 네브라스카 등 14주에서 시험이 이루어 졌다. 6. 미국 USDA의 PMP 재배 지침에 의하면 PMP의 재배생산에는 안전성확보를 위한 별도의 전용장비를 필요로 하며 파종에서 생산까지 엄격하게 관리된다. 7. 미국 USDA의 APHIS는 2002년 PMP 옥수수를 생산하는 ProdiGene, Inc사에 대하여 2건의 포장시험허가 위반사항을 적발하여 벌금 조치를 한바있다. Plant-made pharmaceuticals(PMPs) are therapeutic agents(pharmaceutical proteins) produced in genetically engineered plants. To yield these valuable products plants offer several advantages that include large-scale production capacity at a reduced cost, equivalent purity/activity to other manufacturing systems, and freedom from contamination with animal pathogens, prions, or disease-causing germs. But there are risks of contamination of the food supply and unintended effects on ecosystems. In the U.S. PMPs are regulated under stringent requirement of the U.S. Department of Agriculture(USDA) and the Food and Drug Administration(FDA). In this article, contamination incident with PMP corn engineered by ProdiGene, Inc will be briefly introduced and regulations of PMPs will be discussed.