2004년 7월 봇돌바다의 영양염과 Chlorophyll-a의 단기 변동

최용규,조은섭,권기영,이용화,이영식 한국환경과학회 2005 한국환경과학회지 Vol.14 No.4

In order to study the temporal variations of nutrients and chlorophyll-a in the Bottol Bada, three field observations were carried out on 20, 23 and 26 July, 2004. The low N:P values exhibit nitrogen deficiency during the periods of observation. This result is not representative of typical summer environment in the southern coast of Korea. The possible mechanisms are as follows: 1) The freshwater inflow was not sufficient for the supply of nitrogen because the total precipitation was 11.9 mm in July, 2004. This amount is no more than 5% in normal precipitation in July. 2) There was an inflow of oceanic water under the subsurface into the Bottol Bada. Even though the oceanic water comprises more nutrients, it produces the stratification between the surface and the subsurface water and seems to prevent the supply of nutrinets to the surface layer. 3) The high chlorophyll-a concentration of 1.2 ㎍/L was shown near the narrow channel between Gae-do and Geumo-do. This seems to be resulted from the inflow of water from Gamak Bay.

양로원 노인에서 인지기능 평가에 대한 연구

최성환,정은기,김유광 大韓神經精神醫學會 1993 신경정신의학 Vol.32 No.6

The aged population is growing rapidly in Korea. In 1989, the people aged 65 and older marked 4.7% of the population and is expected to exceed 5% in 1993. It has been considered that as the elderly grow older, their cognitive function decline. As the proportion they take in the society is becoming larger, the use of more stable and reliable examination tools become more important to deal with and prevent their cognitive dysfunction. This study looked into the characteristics of cognitive functions of the elderly living in nursing homes. Mini-mental status examination and Brief cognitive rating scale were administered to 530 eldery(mean age 76±7.5) residing in 8 nursing homes located in Kyun-Gi province, vicinity of Seoul, in 1989 and in 1991. Cognitive function correlated with educational level, but not with age. The correlation of MMSE with BCRS was high. According to the results, it was not evident that aging deteriorates cognitive function. The deterioration of cognitive function was related to educational level. MMSE and BCRS were considered appropriate instruments in brief assessment of cognitive function and its prognosis in the elderly. Lengthened follow up interval and the cognitive evaluation of more various groups are desired in the future studies.

기하광학영역에서의 능동소나 표적신호합성

신기철,박재은,김재수,최상문,김우식 한국해양대학교 해양과학기술연구소 2001 硏究論文集 Vol.10 No.1

능동소나 시스템에서 표적신호의 근거리 정보가 중요해짐에 따라, 표적 음향산란 신호의 고유한특성을 분석하기 위해 실험적인 방법과 시뮬레이션 기법이 사용된다. 본 논문에서는 표적신호 합성을 위해 축소표적 반향실험을 수행하였고, 자료의 분석 결과 축소표적의 음향산란은 거울면 반사의 영향이 큰 것으로 분석되어, 표적신호 합성에서 기하광학이론을 응용하였다. 복잡한 형태의 표적을 여러 표본형상으로 분리하고, 각 표본형상의 기여도를 신호의 위상과 강도를 줌으로써 계산하였으며. 표적신호 합성모델의 검증을 위해 실제 축소표적 실험 결과와 비교하였다. 혁심용어: 기하광학이론. 음향산란, 거울면반사. 축소표적, 능동소나 투고분야: 수중음향 분야 (5.2) Since the near field iformation of target signal is important in the development and verification of active sonar system experimental method and simulation technique are widely used in order to analyze the detail characteristics of target scattered echoes. Therefore, in this paper, the scale target experiment is performed to develope and improve the target signal simulation model. Since the experimental results show that the specular reflection is the major component among scattering mechanisms, the target signal simulation model based on the Geometric Optics Theory (GOT) is developed. Complex target is separated into simple shapes, known as canonical shape. The contribution from individual canonical shapes are summed with proper phase and amplitude to produse the target strength of the whole complex body. Simulated target signal is compared with the experimental results fed discussed.

Ebb and Flood System을 이용한 팔레높시스 양액 재배에서 배양액 농도에 따른 묘 생육 및 양분흡수 패턴

한은주,최영일,백기엽 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

안구의 비색소성 모양체 상피에서 발생한 유두상 선암종 -1예 보고-

최현주,최영진,이연수,이은정,강석진,김병기,심상인 대한병리학회 1998 Journal of Pathology and Translational Medicine Vol.32 No.12

열안정형 카테콜-O-메틸전이효소 유전자의 클로닝

홍경만,최용복,정갑용,지은정,장현신,박현,백문기 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Catechol-O-methyltransferase (COMT; EC 2.1.1.6) is the enzyme which catalyzes the transfer of methyl group to the catecholamine neurotransmitters from its methyl donor S-adenosyl-L-methionine. Partially purified COMT from rat liver has been used to measure the concentration of catecholamines in the blood through solvent extraction and thin layer chromatography after converting ^3H-methyl derivatives of catecholamines. To improve this inconvenient and fluctuating method, an attempt was made to use COMT gene for the measurement of catecholamines. Specific primers, COMT5P (5'-TGC TCA GAG GTG CTT TGA AG-3') and COMT3P (5'-GGA GCC GCA GAA GGT CA G-3'), were used to amplify COMT gene from human placenta cDNA library. The amplified COMT gene through 35 cycles of polymerase chain reaction was cloned into T-vector and the nucleotide sequences are determined by automatic sequencer. Human COMT has two common variants, a thermostable high activity form which has valine at amino acid 158 and a thermolabile low activity form which Has methionine at the same position. The cloned COMT gene in this study has both variants. Clone 4 and 5 have valine at amino acid 158 (guanine at nucleotide 472) and clone 1-3 have methionine at this site (adenine at nucleotide 472). In addition to this, there are other DNA polymorphisms in COMT gene at nucleotide 101, 102 (amino acid 34, a structural mutation cysteine/serine) and nucleotide 186 (a silent mutation). All of the clones have cysteine at amino acid 34 (guanine and adenine at nucleotides 101 and 102) and cytosine at nucleotide 186 except clone 3 which has thymine at this nucleotide. Clone 4 and 5 are thermostable high activity variants, suggesting being more useful for the measurement of catecholamines after expressing the gene.

n-CdS_(0.46)Se_(0.54)/p-Cu_92-x)S_(0.46)Se_(0.54) 이종접합 태양전지의 제작과 그 특성에 관한 연구

유상하,최승평,이상열,홍광준,서상석,김혜숙,전승룡,윤은희,문종대,신영진,정태수,신현길,김택성,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

승화방법에 의해 CdS_0.46Se_0.54 단결정을 성장하여 결정구조를 조사하고, Van der Pauw 방법으로 Hall effect를 측정하여 carrier density의 온도 의존성과 mobility의 온도 의존성을 조사하였다. 성장된 CdS_0.46Se_0.54 단결정을 치환반응하여 n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 이종접합 태양전지를 제작하였다. Spectral response, 전류-전압특성 및 전력변환 효율을 조사하여 그 결과로부터 개방전압은 0.48V, 단락 전류 밀도는 21mA/㎠, fill factor와 전력변환효율은 각각 0.75와 9.5%를 얻었다. CdS_0.46Se_0.54 single crystal was grown by a sublimation method. The crystal structure and the temperature dependence of carrier density and mobility of CdS_0.46Se_0.54 single crystal were studied. Heterojunction solar cells on n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 were fabricated by the substitution reaction. The spectral response, the J-U characteristics and the conversion efficiency of the n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells were studied. The open-cricuit voltage, short-circuit density, fill factor and conversion efficiency of n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells under 80mW/㎠ illumination were found to be 0.48V, 21mA/㎠, 0.75 and 9.5%, respectively.

B형 간염 동반 원발성 간암 환자에서의 CYP2D6와 NAT2 다형현상에 관한 연구

신동일,이광수,최연남,황기은,조율희 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.2

The CYP2D6 and N-acetyltransferase (NAT2) are polymorphic enzymes which are expressed in the hepatocyte in a genotype-determined manner. CYP2D6 and NAT2 are known to be involved in the inactivation and activation of various mutagens and carcinogens, respectively. The activities of the two enzyme systems are associated with the genetic susceptibility of many human cancers. This study was performed to determine the genotype frequencies of the two enzyme systems in primary hepatocellular carcinoma patients and healthy controls. One hundred healthy controls and 55 liver cancer patients were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The results are as followings:1. In the healthy controls, CYP2D6 wild type allele frequency was 0.985 and CYP2D6^*4 frequency was 0.015, and no CYP2D6 poor-metabolizer was detected. No significant differences were found in hepatocellular carcinoma patient group. 2. Frequencies of F, S1, S2 and S3 alleles of NAT2 system were 0.725, 0.01, 0.14 and 0.125 in controls, respectively. The genotype frequencies were found to be 0.91 of rapid acetylator and 0.09 of slow acetylator. No significant differences were found in hepatocellular carcinoma patient group. 3. These results suggest that the distribution of CYP2D6 and NAT2 polymorphisms is very unique in Korean populations characterized by extremely low frequency of CYP2D6 poor-metabolizer and NAT2 slow acetylator. CYP2D6 and NAT2 polymorphisms seemed not to play an important role in the hepatic carcinogenesis in Korean population.

만성 호중구성 백혈병 1예

박기령,조성민,우가은,이기현,손혜영,임정윤,최진혁,이순남,정화순 梨花女子大學校 醫科大學 醫科學硏究所 1997 EMJ (Ewha medical journal) Vol.20 No.2

Chronic neutrophilic leukemia(CNL) is a very rare myeloproliferative disease, characterized by sustained mature neutrophilic leukocytosis with granulocytic bone marrow infiltration, high NAP(neutrophilic alkaline phosphatase) score and absence of philadelphia chromosome, It is frequently accompanied by hepatosplenomegaly, elevated serum vitamin B_12 and uric acid level. For the diagnosis of CNL, the leukemoid reaction, especially secondary to neoplasia, infection and autoimmune diseases, should be excluded. Since Tuohy's first description in 1920, more than 50 cases fullfilling the above criteria have been reported worldwide, and 4 cases in Korea. Several authors have demonstrated the defect of intracellualr killing in the mature neutrophil and this finding correlates well with the very high incidence of fatal infection, The hemorrhagic diasthesis in CNL is caused by functional abnormality of the platelet. This disease has tendency to transform to blastic crisis and acute leukemia as in other myeloproliferative disease, but characteristically shows frequent coexistence with multiple myeloma. Until now , the therapeutic trials in CNL have been disappointing. Hydroxyrea and busulfan can control hyperleukocytosis. On the basis of functional defect in neutrophil, alpha-2b-interferon has been tried and several reports have demonstrated the clinical and functional effect of interferon on CNL. CNL is very rate hematologic disease and there are few report about general aspect of disorder. We report here a typical CNL case presenting with splenomegaly and leukocytosis with a review of the literature.

정신분열병과 N-Methyl-D-Aspartate 수용체 유전자와 연관된 VNTR 표지자인 D9S158 다형성의 연합

김종원,채영규,정은기,김길숙,박강규,이영호,최인근,양병환,한진희 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

정신분열병과 NMDA 수용체와 연관된 D9S158 유전적 표지자와의 연관성을 알아보기 위해 75명의 정신분열병 환자군과 87명의 정상대조군을 대상으로 중합효소연쇄반응을 이용하여 연합연구를 시행하여 다음과 같은 결론을 얻었다. D9S158 유전좌위에는 모두 8개의 대립유전자가 있었고, 각 대립유전자는 cytosine-adenine염기가 반복되는 217염기쌍에서 233염기쌍의 형태였다. 먼저 정신분열병 환자와 정상대조군의 대립유전자의 빈도를 비교해 본 결과 통계적으로 유의한 차이를 보이지 않았다(p〉.05). 환자군을 좀 더 동질적으로 만들기 위해 정신분열병의 가족력 유무, 음성 및 양성증상, 연성신경학적 징후, DSM-IV 임상아형에 따라 환자군을 나누어 대립유전자의 빈도를 비교해 본 결과, 특정 임상양상과 관련이 있는 대립유전자를 발견하지 못하였다. 결론적으로 본 연구에서는 D9S158 유전좌위와 정신분열병의 연관성을 입증하지 못하였으나 NMDA 수용체 유전자주위의 다른 표지자나 좀 더 유전적으로 동질한 환자군을 대상으로 한 추후연구가 필요할 것으로 생각된다. An association study with Korean schizophrenic patients(N=75) and normal controls(N=87) was performed to find the relationship between D9S158 polymorphism and schizophrenia using polymerase chain reaction. Eight different alleles of a dinucleotide polymorphism on D9S158 locus were observed in both group. When we compared the frequencies of alleles between schizophrenics and normal controls, there was no significant difference between two groups. To increase homogeneity of schizophrenic group, we divided schizophrenic group by clinical phenotypes such as family history, negative and positive symptoms(PANSS), soft neurologic signs(NES-K) and DSM-IV diagnostic subtypes. Then we compared the frequencies of alleles among subgroups of clinical phenotypes, and there were no significant differences between subgroups(p〉.05). Although our findings fail to provide an evidence of association between schizophrenia and D9S158 locus, further investigation of other loci that are linked to NMDA receptor gene may be needed in genetically homogeneous subgroups of schizophrenia.